scholarly journals Screening and Identification for Immunological Active Components from Andrographis Herba Using Macrophage Biospecific Extraction Coupled with UPLC/Q-TOF-MS

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1047 ◽  
Author(s):  
Yaqi Wang ◽  
Jiaojiao Jiao ◽  
Yuanzhen Yang ◽  
Ming Yang ◽  
Qin Zheng
Keyword(s):  
Active Components ◽  
Screening And Identification ◽  
Tof Ms

scholarly journals An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

2021 ◽  
Vol 12 ◽  
Author(s):  
Rong Dong ◽  
Qingping Tian ◽  
Yongping Shi ◽  
Shanjun Chen ◽  
Yougang Zhang ◽  
...  
Keyword(s):  
Liver Injury ◽  
Evaluation System ◽  
Multiple Infection ◽  
Active Components ◽  
Grey Correlation ◽  
Liver Protection ◽  
Quality Markers ◽  
Anti Inflammatory ◽  
Highly Correlated ◽  
Tof Ms

Background: Gardenia Fructus (GF), a traditional Chinese medicine of Gardenia Ellis in Rubiaceae family, has the potential to clear heat and purge fire and has been widely used to treat multiple infection-related diseases. However, the quality markers (Q-Markers) of GF have not been revealed comprehensively.Methods: In this experiment, the transgenic zebrafish lines, Tg (l-fabp:EGFP) and Tg (lyz:EGFP), were used to evaluate two main kinds of traditional efficacies of GF, hepatoprotective and anti-inflammatory effects. All the GF samples from different production areas were tested their anti-liver injury and anti-inflammantory activities. High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method (HPLC-Q-TOF/MS) was employed for herbal metabonomic analysis of GF samples. Gray correlation analysis (GCA) was utilized to screen out the components closely associated with the activities. Finally, the zebrafish model was applied to verify the bioactivity of the crucial components to determine the Q-Markers of GF.Results: The zebrafish models were established by inducing with hydrogen peroxide or copper sulfate and applied to quickly evaluate the hepatoprotective effect and inflammation of GF samples. 27 potentially active components for liver protection and 21 potentially active components with anti-inflammatory properties were identified by herbal metabolomic analysis based on HPLC-Q-TOF/MS. The GCA result showed that five of the 27 components were highly correlated with liver protection, 15 of the 21 components were highly correlated with anti-inflammatory activity. Among them, geniposide and crocin-1 were confirmed their bioactivities on zebrafish experiment to be responsible for the protective effects of GF against liver injury, and genipin-1-β-D-gentiobioside, quinic acid, gardenoside, d-glucuronic acid, l-malic acid, mannitol, rutin, and chlorogenic acid were confirmed to be responsible for the anti-inflammatory effects. Finally, according to the screening principles of Q-Markers, genipin-1-β-D-gentiobioside, geniposide, and gardenoside were preliminarily identified to be the Q-Markers of GF.Conclusion: This study established an effective research strategy of “Omics Discrimination-Grey Correlation-Biological Verification,” which enabled the rapid identification of key pharmacological components of GF. These markers have provided a scientific basis for constructing a modern quality evaluation system for GF.

A pre-classification strategy based on UPLC-Triple-TOF/MS for metabolic screening and identification of Radix glehniae in rats

2016 ◽  
Vol 408 (26) ◽  
pp. 7423-7436 ◽  
Author(s):  
Shuang Wang ◽  
Pengcheng Qi ◽  
Na Zhou ◽  
Minmin Zhao ◽  
Weijing Ding ◽  
...  
Keyword(s):  
Screening And Identification ◽  
Metabolic Screening ◽  
Classification Strategy ◽  
Tof Ms

Screening and identification of potential active components in Ophiopogonis Radix against atherosclerosis by biospecific cell extraction

2019 ◽  
Vol 1133 ◽  
pp. 121817 ◽  
Author(s):  
YuShan Tian ◽  
PuYang Gong ◽  
Yan Wu ◽  
Shanquan Chang ◽  
Juntao Xu ◽  
...  
Keyword(s):  
Active Components ◽  
Cell Extraction ◽  
Screening And Identification

scholarly journals Studies on the Mechanism of Glutamate Metabolism in NTG-Induced Migraine Rats Treated with DCXF

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Ni Ni ◽  
Qingqing Wang ◽  
Xiao Lin ◽  
Yanlong Hong ◽  
Yi Feng ◽  
...  
Keyword(s):  
Metabolic Profile ◽  
Amino Acid Metabolism ◽  
Time Trend ◽  
Acid Metabolism ◽  
Glutamate Metabolism ◽  
Active Components ◽  
Serum Samples ◽  
Time Points ◽  
Gs Protein ◽  
Tof Ms

Objective. To explore the mechanism of the antimigraine effect by active components extracted from the Dachuanxiong prescription (DCXF), nitroglycerin- (NTG-) induced migraine rats were used to detect the change of glutamate metabolism and the overall metabolic profile at different time points in the serum and Trigeminocervical complex(TCC) samples. Method. The biological samples that were obtained at 30 minutes, 60 minutes, and 90 minutes after model establishment or drug administration were tested by GC-TOF-MS. Then, real-time PCR and western blot were applied to detect changes in the expression of some substances involved in glutamate metabolism. Result. DCXF could improve the metabolic profile of serum and TCC in migraine rats and showed the time trend of treatment, mainly involved by amino acid metabolism (glutamate, aspartic acid, and alanine metabolism). In addition, DCXF could increase the expressions of GS at 60 min and 90 min and EAAT1 at 90 min. The results of GS protein were similar to that of mRNA. Conclusion. The antimigraine effect of DCXF could be achieved by improving the metabolic profile and increasing the expressions of GS and EAAT1 to promote the glutamate cycle of TCC and serum samples in NTG-induced migraine rats to a certain extent.

scholarly journals Screening and identification of the active components from Puerariae Radix by HUVEC/CMC-LC-MS2

2019 ◽  
Vol 1132 ◽  
pp. 121825
Author(s):  
Yan-Ni Zhang ◽  
Si-Jin Zhu ◽  
Na Li ◽  
Yan-Nan Jing ◽  
Xuan-Feng Yue
Keyword(s):  
Active Components ◽  
Puerariae Radix ◽  
Screening And Identification

scholarly journals Screening and Identification of Multi-Components in Re Du Ning Injections Using LC/TOF-MS Coupled with UV-Irradiation

2014 ◽  
Vol 53 (5) ◽  
pp. 778-786 ◽  
Author(s):  
Lingzhi Li ◽  
Zhenzhong Wang ◽  
Ying Peng ◽  
Xiaohuan Fu ◽  
Yongxiang Wang ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Screening And Identification ◽  
Tof Ms

scholarly journals Identification of “Multiple Components-Multiple Targets-Multiple Pathways” Associated with Naoxintong Capsule in the Treatment of Heart Diseases Using UPLC/Q-TOF-MS and Network Pharmacology

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Xianghui Ma ◽  
Bin Lv ◽  
Pan Li ◽  
Xiaoqing Jiang ◽  
Qian Zhou ◽  
...  
Keyword(s):  
Drug Absorption ◽  
Heart Diseases ◽  
Network Pharmacology ◽  
Chemical Compositions ◽  
Active Components ◽  
Multiple Targets ◽  
Multiple Components ◽  
Main Components ◽  
Tof Ms ◽  
Universal Protein

Naoxintong capsule (NXT) is a commercial medicinal product approved by the China Food and Drug Administration which is used in the treatment of stroke and coronary heart disease. However, the research on the composition and mechanism of NXT is still lacking. Our research aimed to identify the absorbable components, potential targets, and associated pathways of NXT with network pharmacology method. We explored the chemical compositions of NXT based on UPLC/Q-TOF-MS. Then, we used the five principles of drug absorption to identify absorbable ingredients. The databases of PharmMapper, Universal Protein, and the Molecule Annotation System were used to predict the main targets and related pathways. By the five principles of drug absorption as a judgment rule, we identified 63 compositions that could be absorbed in the blood in all 81 chemical compositions. Based on the constructed networks by the significant regulated 123 targets and 77 pathways, the main components that mediated the efficacy of NXT were organic acids, saponins, and tanshinones. Radix Astragali was the critical herbal medicine in NXT, which contained more active components than other herbs and regulated more targets and pathways. Our results showed that NXT had a therapeutic effect on heart diseases through the pattern “multiple components-multiple targets-multiple pathways.”

scholarly journals Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Xin Shao ◽  
Jie Zhao ◽  
Xu Wang ◽  
Yi Tao
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Chemical Constituents ◽  
Rapid Screening ◽  
Active Components ◽  
Limit Of Quantification ◽  
Unknown Compound ◽  
Salvianolic Acid ◽  
Tof Ms

Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy. However, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound. Among these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280, and 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.

On-Line Screening and Identification of Radical Scavenging Compounds Extracted from Flos Lonicerae by LC-DAD–TOF-MS

2008 ◽  
Vol 68 (5-6) ◽  
pp. 327-332 ◽  
Author(s):  
Yan Chen ◽  
Shaoguang Li ◽  
Xinhua Lin ◽  
Hongbin Luo ◽  
Guangwen Li ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Flos Lonicerae ◽  
Screening And Identification ◽  
On Line ◽  
Radical Scavenging Compounds ◽  
Tof Ms
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