scholarly journals Single Laboratory Validation of a Quantitative Core Shell-Based LC Separation for the Evaluation of Silymarin Variability and Associated Antioxidant Activity of Pakistani Ecotypes of Milk Thistle (Silybum Marianum L.)

Molecules ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 904 ◽  
Author(s):  
Samantha Drouet ◽  
Bilal Abbasi ◽  
Annie Falguières ◽  
Waqar Ahmad ◽  
Sumaira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Milk Thistle ◽  
Core Shell ◽  
Silybum Marianum ◽  
Single Laboratory

scholarly journals A New Class of Synthetic Flavonolignan-Like Dimers: Still Few Molecules, but with Attractive Properties

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 108 ◽  
Author(s):  
Valeria Romanucci ◽  
Giovanni Di Fabio ◽  
Armando Zarrelli
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Chemistry ◽  
Biological Response ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
New Class ◽  
Therapeutic Value ◽  
Recent Developments

In recent years, there has been increasing interest in dimeric molecules due to reports of their promising therapeutic value in the treatment of numerous diseases (such as cancer, HIV, Alzheimer’s and, malaria). Many reports in the literature have highlighted the ability of these molecules to interact not only with specific biologic receptors but also to induce a biological response that more than doubles the results of the corresponding monomeric counterpart. In this regard, flavonolignan dimers or simply bi-flavonolignans are an emerging class of dimeric compounds that unlike bi-flavonoids, which are very widespread in nature, consist of synthetic dimers of some flavonolignans isolated from the milk thistle Silybum marianum [L. Gaertn. (Asteraceae)]. This mini-review will discuss recent developments in the synthesis, characterization and antioxidant activity of new families of flavonolignan dimers, in light of emerging medicinal chemistry strategies.

scholarly journals Comparative study of the antioxidant properties of silymarin and milk thistle extracts

2021 ◽  
Vol 19 (1) ◽  
pp. 1-6
Author(s):  
V. Ivanov ◽  
M. Pavlova
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Milk Thistle ◽  
Flavonoid Content ◽  
Silybum Marianum ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Purpose: Milk thistle (Silybum marianum (L.) Gaertn) has been used for centuries as a natural remedy for liver and gall bladder / biliary tract diseases. Silymarin is the purified and standardized extract from milk thistle seeds. The purpose of the present study is to present a comparative analysis of the total phenolic substances content and the antioxidant effect of silymarin and three different types of Silybum marianum extracts (i.e. ethanol extract, water extract and lyophilized extract). Methods: Total phenolic and flavonoid content of extracts was analysed and the antioxidant activity was assessed by determining their free radical scavenging ability by DPPH method. Results: Total phenolic content is the highest in silymarin– 134.12±3.22 mg GAE/g, followed by lyophilized water extract – 51.74±2.10 mg GAE/g. Silymarin has also been found to have the highest antioxidant activity among all extract samples (338.03 VCEAC µmol /g), followed by S.marianum lyophilised extract (123.05 VCEAC µmol/g). Conclusions: A strong correlation between total phenolic and flavonoid content in the extracts and their antioxidant activity has been found.

scholarly journals Single Laboratory Validation of UHPLC-MS/MS Assays for Six Milk Thistle Flavonolignans in Human Serum

2020 ◽  
Author(s):  
Ruth N Muchiri ◽  
Richard B van Breemen
Keyword(s):  
Quantitative Analysis ◽  
Human Serum ◽  
Quantitative Method ◽  
Extraction Efficiency ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
Coefficients Of Variation ◽  
High Concentrations ◽  
Method Accuracy ◽  
Single Laboratory

Abstract Background Extracts of milk thistle, Silybum marianum (L.) Gaertn., are used as dietary supplements for their hepatoprotective, anti-inflammatory, and anti-tumor activities. Objective An assay based on UHPLC-MS/MS was developed and validated for the quantitative analysis of six major milk thistle flavonolignans extracted from human serum. Methods Ethyl acetate containing 0.1% formic acid was used to extract flavonolignans from human serum. A 10-min UHPLC-MS/MS method using selected reaction ion monitoring was developed for measuring extracts for silybin A, silybin B, isosilybin A, isosilybin B, silychristin, and silydianin. Results The quantitative method was validated with respect to selectivity, specificity, accuracy, linearity, precision, LOD, and LLOQ. Extraction efficiency for the quality control standards at LLOQ, low, medium, and high concentrations ranged between 81% and 109%, and the calibration curves were linear (R2 > 0.997) for all flavonolignans. The method precision was determined using coefficients of variation, which were <15%. The method accuracy was assessed using percent relative error which was <15%. Conclusions The UHPLC-MS/MS assay is fast, precise, sensitive, selective, accurate, and useful for the analysis of milk thistle flavonolignans in human serum. Highlights The UHPLC-MS/MS assay is suitable for rapid quantitative analysis of milk thistle flavonolignans in human serum.

The pathogen of Milk Thistle (Silybum marianum (L.) Gaertn.) in the Czech Republic

2015 ◽  
Vol 5 (1) ◽  
pp. 538-544
Author(s):  
Ivana Safrankova ◽  
Kolackova P ◽  
Rutivckova G
Keyword(s):  
Czech Republic ◽  
Environmental Factors ◽  
Active Compound ◽  
Fungal Species ◽  
Medicinal Herb ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
The Czech Republic

Milk thistle is grown in the Czech Republic as a medicinal herb; silymarin is isolated from its achenes and used for the production of liver and gallbladder medicine. The quality and content of the active compound is influenced not only by environmental factors, but also by pests and pathogens. The occurrence of pests of milk thistle variety Silyb was observed in two localities during the years 2011 2013. In the year 2011 the mycoflora of seeds of four milk thistle varieties was determined. Representatives of 15 species were isolated from the seeds, most of them saprophytic. 21 fungal species were isolated and identified from milk thistle plants during the vegetation; Septoria silybi among the most important ones. Possibilities of protection of milt thistle against pathogens are discussed.

scholarly journals TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF SILYBUM MARIANUM CULTURES GROWN ON DIFFERENT GROWTH REGULATORS

2017 ◽  
Vol 9 (4) ◽  
pp. 44 ◽  
Author(s):  
Hattem Mekky ◽  
Samah El Sohafy ◽  
Rasha Abu El-khair ◽  
Amr El Hawiet
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Growth Regulators ◽  
Phenolic Content ◽  
Wild Plant ◽  
Total Phenolic ◽  
Silybum Marianum ◽  
Polyphenolic Content ◽  
Trolox Equivalent ◽  
Total Polyphenolic Content

Objective: The present study was designed to study the effect of supplementing Silybum marianum cultures with different combinations and concentrations of growth regulators on the total phenolic content and the antioxidant activity of the produced cultures.Methods: The total polyphenolic content of 23 extracts of calli was calculated as µg of gallic acid/mg of dry weight extract (DWE) using Folin–Ciocalteu reagent. The antioxidant activity was calculated as trolox equivalent antioxidant capacity using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay for extracts containing more than 10 µg gallic acid/mg DWE.Results: Most of the combinations showed an increase in the total polyphenolic concentration compared to the wild plant. However, cultures grown on media supplemented with combinations of benzyl aminopurine (BAP) and gebberellic acid (Gb), adenine (Ad) and kinetin (Kin), BAP and indole acetic acid (IAA) possessed the highest total polyphenolic contents 20.5, 13.09 and 12.15 µg gallic acid/mg DWE, respectively. However, only (BAP+Gb), (BAP+1-Naphthaleneacetic acid (NAA), IAA, kin and (BAP+Ad) showed a significant increase in the antioxidant activity calculated as trolox equivalent with 2.65, 1.56, 1.56, 1.35 and 1.22 folds increase over the wild plant, respectively.Conclusion: The results obtained clearly indicated that changing the growth regulator system of Silybum marianum cultures serves as an easy and reliable method for increasing the total phenolic content and the antioxidant activity of cultures.

scholarly journals Protective Effects of Milk Thistle (Silybum marianum) against Aflatoxin B1 in Broiler Chicks

2011 ◽  
Vol 24 (7) ◽  
pp. 1011-1018 ◽  
Author(s):  
N. Chand ◽  
Din Muhammad ◽  
F. R. Durrani ◽  
M. Subhan Qureshi ◽  
Sahibzada S Ullah
Keyword(s):  
Aflatoxin B1 ◽  
Milk Thistle ◽  
Broiler Chicks ◽  
Protective Effects ◽  
Silybum Marianum

scholarly journals GERMINATION AND SEEDLING GROWTH OF SILYBUM MARIANUM AS A MEDICINAL PLANT UNDER SALINITY STRESS

2019 ◽  
pp. 25-38
Author(s):  
M. A. Dorri ◽  
B. Kamkar ◽  
M. Aghdasi ◽  
A. R. Safahani
Keyword(s):  
Medicinal Plant ◽  
Seedling Growth ◽  
Block Design ◽  
Germination Percentage ◽  
Milk Thistle ◽  
Tolerance Index ◽  
Root Weight ◽  
Silybum Marianum ◽  
Nacl Concentration ◽  
Salinity Levels

Milk thistle (Silybum marianum) is used as a medicinal plant in the treatment of liver diseases. In order to investigate germination and seedling growth in S. marianum subjected to NaCl, a three replicated experiment was carried out in a randomized complete block design in the laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Treatments included seven salinity levels (50, 75, 100, 125, 150, 175 and 200 mM) and a control (distilled water). Germination percentage (GP), mean germination time (MGT), seed-ling vigour index (SVI), shoot, root and seedling weight were measured. The effect of salinity levels was significant on GP, SVI, MGT and seedling weight and length (p ≤ 0.01). However, the NaCl concentration effect was not significant on shoot : root length ratio and or shoot : root weight ratios. Results showed that germination decreased when salinity increased, while MGT increased. MGT was 1.75 times higher than in the control at the highest salt concentration. MGT difference was not significant between 75 mM NaCl and con-trol, while it dramatically increased by increasing the NaCl concentration from 150 to 200 mM NaCl. Reduc-tion slope and salt tolerance index (STI) were estimated for germination (0.54 and 231.9) and seedling stage (0.24 and 237.4). According to the results, milk thistle could be considered as a valuable medicinal plant in fairly salinized areas.

scholarly journals Influence of irrigation and row spacing on the growth, seed yield and active substance of milk thistle (Silybum marianum)

2001 ◽  
Vol 7 (3-4) ◽  
Author(s):  
G. Karimzedah ◽  
R. Omidbaigi ◽  
Bakhshai D.
Keyword(s):  
Medicinal Plant ◽  
Seed Yield ◽  
Active Substance ◽  
Milk Thistle ◽  
Row Spacing ◽  
Silybum Marianum ◽  
Ripe Fruit ◽  
Suitable Amount ◽  
Ancient Times ◽  
Active Substances

Milk thistle (Silybum marianum (L.) Gaertn) has been used in medicine since ancient times, but it has been cultivated as a medicinal plant only in recent decades. The ripe fruit of milk thistle contains flavonoids, which are used to prepare anti-hepatotoxic drugs. The main purpose of this study was to substantiate the effects of irrigation and row spacing on growth, seed yield and the content of active substances (silybin and silymarin) in milk thistle. The results showed that the suitable amount of irrigation was 20 mm and the appropriate row spacing was 25 cm.  

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