scholarly journals Substrate Binding Switches the Conformation at the Lynchpin Site in the Substrate-Binding Domain of Human Hsp70 to Enable Allosteric Interdomain Communication

Molecules ◽  
2018 ◽  
Vol 23 (3) ◽  
pp. 528 ◽  
Author(s):  
Kohei Umehara ◽  
Miho Hoshikawa ◽  
Naoya Tochio ◽  
Shin-ichi Tate
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
Substrate Binding Domain ◽  
Human Hsp70 ◽  
Interdomain Communication

Distinct binding sites for the ATPase and substrate-binding domain of human Hsp70 on the cell surface of antigen presenting cells

2008 ◽  
Vol 45 (15) ◽  
pp. 3974-3983 ◽  
Author(s):  
Sandra Zitzler ◽  
Alice Hellwig ◽  
Franz-Ulrich Hartl ◽  
Felix Wieland ◽  
Petra Diestelkötter-Bachert
Keyword(s):  
Cell Surface ◽  
Binding Sites ◽  
Substrate Binding ◽  
Antigen Presenting Cells ◽  
Binding Domain ◽  
Antigen Presenting ◽  
Substrate Binding Domain ◽  
Human Hsp70

scholarly journals Structure of the substrate-binding domain of Plasmodium falciparum heat-shock protein 70-x

2020 ◽  
Vol 76 (10) ◽  
pp. 495-500
Author(s):  
Julia Schmidt ◽  
Ioannis Vakonakis
Keyword(s):  
Plasmodium Falciparum ◽  
Heat Shock ◽  
Malaria Parasite ◽  
Substrate Binding ◽  
Binding Domain ◽  
Crystallographic Structure ◽  
Hydrophobic Peptide ◽  
Febrile Episodes ◽  
Substrate Binding Domain ◽  
Human Hsp70

The malaria parasite Plasmodium falciparum extensively modifies erythrocytes that it invades by exporting a large complement of proteins to the host cell. Among these exported components is a single heat-shock 70 kDa class protein, PfHsp70-x, that supports the virulence and growth rate of the parasite during febrile episodes. The ATP-binding domain of PfHsp70-x has previously been resolved and showed the presence of potentially druggable epitopes that differ from those on human Hsp70 chaperones. Here, the crystallographic structure of the substrate-binding domain (SBD) of PfHsp70-x is presented in complex with a hydrophobic peptide. The PfHsp70-x SBD is shown to be highly similar to the counterpart from a human erythrocytic Hsp70 chaperone. The binding of substrate at the interface between β-sandwich and α-helical subdomains of this chaperone segment is also conserved between the malaria parasite and humans. It is hypothesized that the parasite may partly exploit human chaperones for intra-erythrocytic trafficking and maintenance of its exported proteome.

scholarly journals Entropic Inhibition: How the Activity of a AAA+ Machine Is Modulated by Its Substrate-Binding Domain

2021 ◽  
Author(s):  
Marija Iljina ◽  
Hisham Mazal ◽  
Pierre Goloubinoff ◽  
Inbal Riven ◽  
Gilad Haran
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
Substrate Binding Domain

scholarly journals Flexible Linkers Leash the Substrate Binding Domain of SspB to a Peptide Module that Stabilizes Delivery Complexes with the AAA+ ClpXP Protease

2003 ◽  
Vol 12 (2) ◽  
pp. 355-363 ◽  
Author(s):  
David A Wah ◽  
Igor Levchenko ◽  
Gabrielle E Rieckhof ◽  
Daniel N Bolon ◽  
Tania A Baker ◽  
...  
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
Substrate Binding Domain

scholarly journals An atomistic view of Hsp70 allosteric crosstalk: from the nucleotide to the substrate binding domain and back

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Federica Chiappori ◽  
Ivan Merelli ◽  
Luciano Milanesi ◽  
Giorgio Colombo ◽  
Giulia Morra
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
Substrate Binding Domain

Rotary and Unidirectional Metal Shadowing of VAT: Localization of the Substrate-Binding Domain

2000 ◽  
Vol 132 (2) ◽  
pp. 162-168 ◽  
Author(s):  
Beate Rockel ◽  
Reinhard Guckenberger ◽  
Heinz Gross ◽  
Peter Tittmann ◽  
Wolfgang Baumeister
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
Substrate Binding Domain

Studies on the effect of the J-domain on the substrate binding domain (SBD) of Hsp70 using a chimeric human J-SBD polypeptide

2019 ◽  
Vol 124 ◽  
pp. 111-120 ◽  
Author(s):  
Ana O. Tiroli-Cepeda ◽  
Thiago V. Seraphim ◽  
Glaucia M.S. Pinheiro ◽  
Denio E.P. Souto ◽  
Lauro T. Kubota ◽  
...  
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
J Domain ◽  
Substrate Binding Domain

Engineering the substrate-binding domain of an esterase enhances its hydrolytic activity toward fatty acid esters

2014 ◽  
Vol 49 (12) ◽  
pp. 2101-2106 ◽  
Author(s):  
Young-A Lee ◽  
Eun-Yeong Jeon ◽  
Sun-Mee Lee ◽  
Uwe T. Bornscheuer ◽  
Jin-Byung Park
Keyword(s):  
Fatty Acid ◽  
Substrate Binding ◽  
Hydrolytic Activity ◽  
Binding Domain ◽  
Fatty Acid Esters ◽  
Substrate Binding Domain ◽  
Acid Esters

Identification of Hsp70 modulators through modeling of the substrate binding domain

2009 ◽  
Vol 19 (14) ◽  
pp. 3828-3831 ◽  
Author(s):  
Conor M. Haney ◽  
Corinne Schneider ◽  
Barbara Beck ◽  
Jeffrey L. Brodsky ◽  
Alexander Dömling
Keyword(s):  
Substrate Binding ◽  
Binding Domain ◽  
Substrate Binding Domain
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