scholarly journals Structural Basis for the Substrate Inhibition of Proline Utilization A by Proline

Molecules ◽  
2017 ◽  
Vol 23 (1) ◽  
pp. 32 ◽  
Author(s):  
David Korasick ◽  
Travis Pemberton ◽  
Benjamin Arentson ◽  
Donald Becker ◽  
John Tanner
Keyword(s):  
Substrate Inhibition ◽  
Structural Basis ◽  
Proline Utilization ◽  
Proline Utilization A

scholarly journals Kinetic and Structural Characterization of Tunnel-Perturbing Mutants in Bradyrhizobium japonicum Proline Utilization A

Biochemistry ◽  
2014 ◽  
Vol 53 (31) ◽  
pp. 5150-5161 ◽  
Author(s):  
Benjamin W. Arentson ◽  
Min Luo ◽  
Travis A. Pemberton ◽  
John J. Tanner ◽  
Donald F. Becker
Keyword(s):  
Structural Characterization ◽  
Bradyrhizobium Japonicum ◽  
Proline Utilization ◽  
Proline Utilization A

scholarly journals Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function

2016 ◽  
Vol 291 (46) ◽  
pp. 24065-24075 ◽  
Author(s):  
Min Luo ◽  
Thameesha T. Gamage ◽  
Benjamin W. Arentson ◽  
Katherine N. Schlasner ◽  
Donald F. Becker ◽  
...  
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Unknown Function ◽  
Aldehyde Dehydrogenase Superfamily ◽  
Proline Utilization ◽  
Domain Of Unknown Function ◽  
Proline Utilization A

scholarly journals Three crystal forms of the bifunctional enzyme proline utilization A (PutA) fromBradyrhizobium japonicum

2008 ◽  
Vol 64 (10) ◽  
pp. 949-953 ◽  
Author(s):  
Jonathan P. Schuermann ◽  
Tommi A. White ◽  
Dhiraj Srivastava ◽  
Dale B. Karr ◽  
John J. Tanner
Keyword(s):  
Bifunctional Enzyme ◽  
Crystal Forms ◽  
Proline Utilization ◽  
Proline Utilization A

scholarly journals Structure, function, and mechanism of proline utilization A (PutA)

2017 ◽  
Vol 632 ◽  
pp. 142-157 ◽  
Author(s):  
Li-Kai Liu ◽  
Donald F. Becker ◽  
John J. Tanner
Keyword(s):  
Structure Function ◽  
Proline Utilization ◽  
Proline Utilization A

scholarly journals Discovery of the Membrane Binding Domain in Trifunctional Proline Utilization A

Biochemistry ◽  
2017 ◽  
Vol 56 (47) ◽  
pp. 6292-6303 ◽  
Author(s):  
Shelbi L. Christgen ◽  
Weidong Zhu ◽  
Nikhilesh Sanyal ◽  
Bushra Bibi ◽  
John J. Tanner ◽  
...  
Keyword(s):  
Membrane Binding ◽  
Binding Domain ◽  
Proline Utilization ◽  
Proline Utilization A

scholarly journals Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA

2016 ◽  
Vol 36 (6) ◽  
Author(s):  
Benjamin W. Arentson ◽  
Erin L. Hayes ◽  
Weidong Zhu ◽  
Harkewal Singh ◽  
John J. Tanner ◽  
...  
Keyword(s):  
Dna Binding ◽  
Modular Design ◽  
Quaternary Structure ◽  
Lipid Binding ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Oligomeric State ◽  
Proline Utilization ◽  
Functional Switching ◽  
Proline Utilization A

Proline utilization A (PutA) is a bifunctional flavoenzyme with proline dehydrogenase (PRODH) and Δ1-pyrroline-5-carboxylate (P5C) dehydrogenase (P5CDH) domains that catalyses the two-step oxidation of proline to glutamate. Trifunctional PutAs also have an N-terminal ribbon–helix–helix (RHH) DNA-binding domain and moonlight as autogenous transcriptional repressors of the put regulon. A unique property of trifunctional PutA is the ability to switch functions from DNA-bound repressor to membrane-associated enzyme in response to cellular nutritional needs and proline availability. In the present study, we attempt to construct a trifunctional PutA by fusing the RHH domain of Escherichia coli PutA (EcRHH) to the bifunctional Rhodobacter capsulatus PutA (RcPutA) in order to explore the modular design of functional switching in trifunctional PutAs. The EcRHH–RcPutA chimaera retains the catalytic properties of RcPutA while acquiring the oligomeric state, quaternary structure and DNA-binding properties of EcPutA. Furthermore, the EcRHH–RcPutA chimaera exhibits proline-induced lipid association, which is a fundamental characteristic of functional switching. Unexpectedly, RcPutA lipid binding is also activated by proline, which shows for the first time that bifunctional PutAs exhibit a limited form of functional switching. Altogether, these results suggest that the C-terminal domain (CTD), which is conserved by trifunctional PutAs and certain bifunctional PutAs, is essential for functional switching in trifunctional PutAs.

scholarly journals Structure and characterization of a class 3B proline utilization A: Ligand-induced dimerization and importance of the C-terminal domain for catalysis

2017 ◽  
Vol 292 (23) ◽  
pp. 9652-9665 ◽  
Author(s):  
David A. Korasick ◽  
Thameesha T. Gamage ◽  
Shelbi Christgen ◽  
Kyle M. Stiers ◽  
Lesa J. Beamer ◽  
...  
Keyword(s):  
Terminal Domain ◽  
Proline Utilization ◽  
Proline Utilization A
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