scholarly journals Sulforaphane Alters β-Naphthoflavone-Induced Changes in Activity and Expression of Drug-Metabolizing Enzymes in Rat Hepatocytes

Molecules ◽  
2017 ◽  
Vol 22 (11) ◽  
pp. 1983 ◽  
Author(s):  
Kateřina Lněničková ◽  
Andrea Dymáková ◽  
Barbora Szotáková ◽  
Iva Boušová
Keyword(s):  
Rat Hepatocytes ◽  
Drug Metabolizing Enzymes ◽  
Metabolizing Enzymes ◽  
Induced Changes

Cryopreserved rat, dog and monkey hepatocytes: measurement of drug metabolizing enzymes in suspensions and cultures

2004 ◽  
Vol 23 (6) ◽  
pp. 307-316 ◽  
Author(s):  
Nicola J Hewitt ◽  
Dietmar Utesch
Keyword(s):  
De Novo ◽  
Rat Hepatocytes ◽  
Cell Suspensions ◽  
Drug Metabolizing Enzymes ◽  
Phase 2 ◽  
Metabolizing Enzymes ◽  
Cells In Culture ◽  
Enzyme Functions ◽  
New Compounds

Metabolism in fresh and cryopreserved (CP) rat, dog and monkey hepatocyte suspensions and cultures was measured using midazolam (CYP3A), tolbutamide (CYP2C), dextromethorphan (CYP2D) and p-nitrophenol (glucuronosyl S-transferases (UGT), sulphotransferases (ST)). CYP3A, CYP2C9, CYP2D6, UGT and ST enzyme functions in fresh and CP rat, dog and monkey hepatocyte suspensions were retained - CP rat hepatocytes lost some CYP2C activity but this was restored by adding NADPH or by placing the cells in culture, suggesting that the enzyme was still functional. Phase 2 activities were equivalent in fresh and CP hepatocyte suspensions. In some cases, incubation conditions increased the rate of metabolism, possibly reflecting de novo cofactor synthesis. However, this effect was substrate and species dependent and was not always the same in fresh and CP cells. CYP3A, CYP2C, CYP2D, UGT and ST activities at 24 hours of culture of rat and monkey hepatocytes were not compromised by cryopreservation. CYP3A, CYP2D but not CYP2C were lower in 24-hour cultures of CP dog hepatocytes than in fresh cells. Despite being lower than fresh cells, UGT activity in dog CP hepatocytes did not decrease from 0 to 24 hours of culture. Speciesspecific metabolism of p-nitrophenol could be demonstrated in both CP cell suspensions and cultures. In conclusion, these data suggest that the enzyme characteristics of fresh and CP hepatocytes from each species and under specific incubation conditions should be considered when carrying out metabolism studies of new compounds.

Long-term preservation and induction of drug metabolizing enzymes in co-cultured rat hepatocytes

1994 ◽  
Vol 22 (2) ◽  
pp. 124S-124S ◽  
Author(s):  
MAY AKRAWI ◽  
VERA ROGIERS ◽  
ANTOINNE VERCRUYSSE ◽  
IAN R. PHILLIPS ◽  
ELIZABETH A. SHEPHARD
Keyword(s):  
Rat Hepatocytes ◽  
Drug Metabolizing Enzymes ◽  
Metabolizing Enzymes ◽  
Cultured Rat Hepatocytes ◽  
Long Term Preservation

Hexachlorocyclohexane (HCH) induced changes in microsomal drug metabolizing enzymes and liver lipids in rats fed different levels of protein

1992 ◽  
Vol 35 (3-4) ◽  
pp. 185-196
Author(s):  
R. N. Khanna ◽  
Rashmi Gupta ◽  
A. Siddiqui ◽  
G. S. D. Gupta ◽  
S. P. Srivastava ◽  
...  
Keyword(s):  
Drug Metabolizing Enzymes ◽  
Liver Lipids ◽  
Metabolizing Enzymes ◽  
Induced Changes ◽  
Different Levels
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