scholarly journals Isaria fumosorosea KCh J2 Entomopathogenic Strain as an Effective Biocatalyst for Steroid Compound Transformations

Molecules ◽  
2017 ◽  
Vol 22 (9) ◽  
pp. 1511 ◽  
Author(s):  
◽  
◽  
◽  
Keyword(s):  
Steroid Compound ◽  
Isaria Fumosorosea

Effects of wetting on the persistence of quiescent conidia of Isaria fumosorosea

2010 ◽  
Vol 55 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Najah Bouamama ◽  
Claire Vidal ◽  
Jacques Fargues
Keyword(s):  
Isaria Fumosorosea

scholarly journals Degradation of the Acyl Side Chain of the Steroid Compound Cholate in Pseudomonas sp. Strain Chol1 Proceeds via an Aldehyde Intermediate

2012 ◽  
Vol 195 (3) ◽  
pp. 585-595 ◽  
Author(s):  
J. Holert ◽  
Z. Kulic ◽  
O. Yucel ◽  
V. Suvekbala ◽  
M. J.- F. Suter ◽  
...  
Keyword(s):  
Side Chain ◽  
Pseudomonas Sp ◽  
Steroid Compound ◽  
Acyl Side Chain

scholarly journals Pathogenicity of Isaria fumosorosea to European red mite in the laboratory

Biotemas ◽  
2017 ◽  
Vol 30 (1) ◽  
pp. 73
Author(s):  
Cláudia Andréia Gräff ◽  
Liana Johann ◽  
Cláucia Fernanda Volken de Souza ◽  
Noeli Juarez Ferla
Keyword(s):  
Rio Grande ◽  
Panonychus Ulmi ◽  
Rio Grande Do Sul ◽  
Isaria Fumosorosea ◽  
European Red Mite

http://dx.doi.org/10.5007/2175-7925.2017v30n1p73A vitivinicultura no estado do Rio Grande do Sul tem sofrido infestações significativas de ácaros praga, destacando-se recentemente Panonychus ulmi (Koch). Há relatos sobre a capacidade de controle de ácaros fitófagos com Isaria fumosorosea. O objetivo deste trabalho foi avaliar em laboratório a ação patogênica de I. fumosorosea sobre o ácaro P. ulmi. As criações de P. ulmi foram estabelecidas a partir de coleta realizadas em videiras da Serra Gaúcha. Sobre os ovos de P. ulmi, as aplicações de suspensões de esporos foram feitas em diferentes concentrações. As triplicatas das fêmeas de 12 a 15 dias foram tratadas com suspensão a 108 esporos x mL-1. As testemunhas foram tratadas com água destilada. Após sete dias da aplicação, observaram-se 55,6% de ovos não eclodidos tratados com suspensão 106 esporos x mL-1 e com fêmeas tratadas obteve-se uma mortalidade total entre 85-90% e mortalidade confirmada entre 50-55%. A mortalidade máxima dos controles no tratamento dos ovos e das fêmeas foi, em média, de 12,8 e 15,5%, respectivamente. Conclui-se que o isolado I. fumosorosea possui habilidade para infectar ovos e fêmeas adultas de P. ulmi e portanto mostra-se como uma alternativa viável para ser experimentada no campo.

scholarly journals Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

2010 ◽  
Vol 53 (4) ◽  
pp. 753-761 ◽  
Author(s):  
Gabriel Moura Mascarin ◽  
Sérgio Batista Alves ◽  
Rogério Biaggioni Lopes
Keyword(s):  
Mass Production ◽  
Culture Media ◽  
Fungal Species ◽  
Complete Medium ◽  
Sugarcane Molasses ◽  
Liquid Media ◽  
Isaria Fumosorosea ◽  
Isaria Farinosa ◽  
Selection For ◽  
Biphasic Fermentation

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and whole rice and broken rice for I. fumosorosea. Results demonstrated that the two fungal species could be rapidly produced with higher yield of conidia on agro-industrial resources by using biphasic fermentation techniques.

scholarly journals KLT BIOAUTOGRAFI HASIL PARTISI EKSTRAK ETANOL HERBA BANDOTAN (Ageratum conyzoides L.) TERHADAP Shigella dysentriae

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Surya Sumantri Abdullah ◽  
Natsir Djide ◽  
Sartini Natsir
Keyword(s):  
Ethyl Acetate ◽  
Incubation Time ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Diameter Measurement ◽  
Steroid Compound ◽  
Ageratum Conyzoides ◽  
Bioassay Method

Penelitian uji daya hambat dan analisis KLT bioautografi hasil partisi ekstrak etanol herba bandotan (Ageratum conyzoides L.) terhadap Shigella dysentriae  telah dilakukan. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak tersebut dalam menghambat pertumbuhan S.dysentriae dan membandingkan daya hambat ekstrak dengan tingkat kepolaran yang berbeda yaitu pada ekstrak n-heksana, larut etil asetat, dan tidak larut etil asetat berdasarkan  pengukurandiameter hambatan yang terbentuk. Herba tersebut diekstraksi dengan etanol menggunakan metode maserasi, lalu dipartisi dengan pelarut n-heksana dan etil asetat. Pengukuran dilakukan dengan menggunakan metode difusi pada medium Miller Hinton Agar (MHA) dengan waktu inkubasi 24 jam pada suhu 37oC dan memberikan diameter daerah hambatan terbesar pada ekstrak tidak larut etil asetat herba bandotan yaitu 9,3 dan 10,3 mm. Pemisahan secara KLT pada ekstrak n-heksana, ekstrak etil asetat, dan ekstrak tidak larut etil asetat dengan cairan pengelusi berturut-turut, n-heksana:etil asetat (3:1), n-heksana:etil asetat (1:1), dan etil asetat:etanol (10:1) dengan jumlah bercak noda berturut-turut 4, 5, dan 2. Nilai Rf pada ekstrak n-heksana 0.25, 0.41, 0.52, dan 0.71 sedangkan pada ekstrak etil asetat 0.34, 0.53, 0.65, 0.81, dan 0.92 pada ekstrak tidak larut etil asetat 0.33 dan 0.64. Hasil KLT bioautografi diperoleh komponen antibakteri yang diidentifikasi ekstrak n-heksana adalah golongan steroid dan pada tidak larut etil asetat golongan polifenol ABSTRACTInhibition test research and TLC bioautographic bioassay method of the partition results of the ethanol extract of bandotan (Ageratum conyzoides L.) herb against Shigella dysentriae have been conducted. This study aims to determine the ability of these extracts to inhibit S.dysentriae growth and to compare the inhibition of extracts with different polarity levels, n-hexane soluble, ethyl acetate soluble, and ethyl acetate insoluble extracts based on the diameter measurement of the formed resistance. The herbs were extracted with ethanol using the maceration method, then partitioned with n-hexane and ethyl acetate solvents. Measurements were carried out using the diffusion method on Miller Hinton Agar (MHA) medium with an incubation time of 24 hours at 37oC and gave the largest diameter area of resistance to the ethyl acetate insoluble extract of bandotan herb, value 9.3 and 10.3 mm. Separation by TLC on n-hexane extract, ethyl acetate extract, and ethyl acetate insoluble extract with elusive liquid respectively, n-hexane: ethyl acetate (3: 1), n-hexane: ethyl acetate (1: 1), and ethyl acetate: ethanol (10: 1) with the number of stains 4, 5, and 2, respectively. 0.81, and 0.92 in ethyl acetate insoluble extracts 0.33 and 0.64. The results of the bioautography TLC bioassay method showed that the antibacterial component identified in the n-hexane extract was a steroid compound and ethyl acetate insoluble was a polyphenol compound.

Evaluation of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for Control of Sitophilus oryzae

2014 ◽  
Vol 77 (1) ◽  
pp. 87-93 ◽  
Author(s):  
NICKOLAS G. KAVALLIERATOS ◽  
CHRISTOS G. ATHANASSIOU ◽  
MARIA M. AOUNTALA ◽  
DEMETRIUS C. KONTODIMAS
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Sitophilus Oryzae ◽  
Exposure Period ◽  
Isaria Fumosorosea ◽  
Stored Grain ◽  
Stored Grain Pest ◽  
Petri Dishes

The entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea were tested against the stored-grain pest Sitophilus oryzae. The fungi were isolated from the soil (from three locations in Attica, Greece: B. bassiana from Tatoion, M. anisopliae from Marathon, and I. fumosorosea from Aghios Stefanos) using larvae of Galleria mellonella as bait. Suspensions of 2.11 × 107 and 2.11 × 108, 1.77 × 107 and 1.77 × 108, and 1.81 × 107 and 1.81 × 108 conidia per ml of B. bassiana, M. anisopliae, and I. fumosorosea, respectively, were applied by three treatments: (i) sprayed on food and set in petri dishes with adults of S. oryzae, (ii) sprayed on adults of S. oryzae and set in petri dishes without food, and (iii) sprayed on adults of S. oryzae and set in petri dishes with food. The observed mortality of S. oryzae adults during the overall exposure period for the lowest, as well as for the highest, concentrations of B. bassiana, M. anisopliae, and I. fumosorosea ranged from 0 to 100%. Concentration was, in most of the cases tested, a critical parameter that determined the “speed of kill” of the exposed insect species for B. bassiana and M. anisopliae. Conversely, concentration was not that critical for I. fumosorosea, and survival was high in some of the combinations tested, even after 14 days of exposure. Both in the highest and the lowest concentrations of fungi, the mortality of S. oryzae adults was higher when the fungi were applied on adults than when they were applied on food. Higher mortality was observed when food was absent than when food was present, in most of the cases tested. The high efficacy levels recorded in the current study indicate that the tested fungi could be effective biocontrol agents against S. oryzae.

scholarly journals Dissemination of Isaria fumosorosea Spores by Steinernema feltiae and Heterorhabditis bacteriophora

2020 ◽  
Vol 6 (4) ◽  
pp. 359
Author(s):  
Jiří Nermuť ◽  
Jana Konopická ◽  
Rostislav Zemek ◽  
Michal Kopačka ◽  
Andrea Bohatá ◽  
...  
Keyword(s):  
Entomopathogenic Nematodes ◽  
Heterorhabditis Bacteriophora ◽  
Potato Dextrose Agar ◽  
Steinernema Feltiae ◽  
Soil Organisms ◽  
Isaria Fumosorosea ◽  
Second Stage ◽  
Glass Tubes ◽  
First Time ◽  
Globally Distributed

Entomopathogenic nematodes and fungi are globally distributed soil organisms that are frequently used as bioagents in biological control and integrated pest management. Many studies have demonstrated that the combination of biocontrol agents can increase their efficacy against target hosts. In our study, we focused on another potential benefit of the synergy of two species of nematodes, Steinernema feltiae and Heterorhabditis bacteriophora, and the fungus Isaria fumosorosea. According to our hypothesis, these nematodes may be able to disseminate this fungus into the environment. To test this hypothesis, we studied fungal dispersal by the nematodes in different arenas, including potato dextrose agar (PDA) plates, sand heaps, sand barriers, and glass tubes filled with soil. The results of our study showed, for the first time, that the spreading of both conidia and blastospores of I. fumosorosea is significantly enhanced by the presence of entomopathogenic nematodes, but the efficacy of dissemination is negatively influenced by the heterogeneity of the testing arena. We also found that H. bacteriophora spread fungi more effectively than S. feltiae. This phenomenon could be explained by the differences in the presence and persistence of second-stage cuticles or by different foraging behavior. Finally, we observed that blastospores are disseminated more effectively than conidia, which might be due to the different adherence of these spores (conidia are hydrophobic, while blastospores are hydrophilic). The obtained results showed that entomopathogenic nematodes (EPNs) can enhance the efficiency of fungal dispersal.

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