scholarly journals A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae)

Molecules ◽  
2017 ◽  
Vol 22 (1) ◽  
pp. 118 ◽  
Author(s):  
Norma Moreno-Anzúrez ◽  
Silvia Marquina ◽  
Laura Alvarez ◽  
Alejandro Zamilpa ◽  
Patricia Castillo-España ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Transformed Hairy Roots ◽  
Anti Inflammatory

scholarly journals Production of the Anticancer Compound Withaferin A from Genetically Transformed Hairy Root Cultures of Withania Somnifera

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Zeynab Yousefian ◽  
Behnaz Hosseini ◽  
Hassan Rezadoost ◽  
Javier Palazón ◽  
Mohammad Hossein Mirjalili
Keyword(s):  
Hairy Roots ◽  
Withania Somnifera ◽  
Growth Index ◽  
Dry Weight ◽  
Squalene Synthase ◽  
Withaferin A ◽  
Farnesyl Pyrophosphate ◽  
Transformed Hairy Roots ◽  
Transformed Hairy Root ◽  
Anticancer Compound

Withanolides (WTDs), well-known medicinally important compounds of Withania somnifera, including the anticancer compound withaferin A (WFA), are biosynthesized from their dedicated precursor squalene. Condensation of farnesyl pyrophosphate (FPP) molecules to produce squalene is catalyzed by squalene synthase (SQS). In the present study, the Arabidopsis thaliana squalene synthase gene ( AtSQS1) was transformed in W. somnifera by Agrobacterium tumefaciens C58C1 (pRiA4). The AtSQS1 gene was detected in 87.88 % of induced transformed hairy roots (THRs). The results showed that the growth index (GI) of THRs after five weeks of culture was 2-fold higher than that of adventitious hairy roots (AHRs). The biomass doubling time of THRs and AHRs was 18 and 30 days, respectively. Quantitative evaluation of WFA in the studied roots showed that THRs contain 1.51-fold more WFA (330±0.87μg g−1 dry weight (DW)) than AHRs (218±0.17μg g−1 DW). These findings can be used for the production of WFA as a valuable anticancer compound through controlled and scaled up cultures of W. somnifera THRs.

scholarly journals Morpho-histological and bioherbicidal evaluation of wild-type and transformed hairy roots of goosefoot

2015 ◽  
Vol 96 ◽  
pp. 53-61 ◽  
Author(s):  
S. Dmitrović ◽  
N. Mitić ◽  
S. Budimir ◽  
D. Janošević ◽  
S. Živković ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Wild Type ◽  
Transformed Hairy Roots

scholarly journals Anti-inflammatory Compounds Produced in Hairy Roots Culture of Sphaeralcea Angustifolia

2021 ◽  
Author(s):  
Rogelio Reyes-Pérez ◽  
Maribel Herrera-Ruiz ◽  
Irene Perea-Arango ◽  
Fernando Martínez-Morales ◽  
José De Jesús Arellano-García ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Acid Production ◽  
Growth Index ◽  
Joint Inflammation ◽  
Wild Plants ◽  
Nodal Segments ◽  
Gastric Disease ◽  
High Transformation ◽  
Anti Inflammatory

Abstract Sphaeralcea angustifolia (Cav) G. Don is used in traditional Mexican medicine to treat inflammations and gastric disease. Its anti-inflammatory and immunomodulatory activities in mice and rats acute and chronic models have been attributed mainly to scopoletin. Scopoletin reduced joint inflammation, the number of new vessels, production of endogenous angiogenic inducers, and reversed the histopathological alterations in rat adjuvant-induced arthritis. Tomentin and sphaeralcic acid from S. angustifolia cells in suspension proved anti-inflammatory and immunomodulatory activities in mice kaolin/λ-carrageenan-induced arthritis. Transformed roots of S. angustifolia have been proposed as active compounds producers. A high transformation frequency mediated by Agrobacterium rhizogenes ATCC15834/pTDT was obtained from nodal segments (59.5 ± 10.5%, 145 hairy root lines) and leaves (40.0 ± 25, 52 hairy root lines) of 2-month-old plantlets. Among seven lines selected according to their phenotypic characteristics and growth index, the SaTR N7.2 line presented the highest sphaeralcic acid production (17.6 ± 1.72 mg/g DW); this production was 440-fold superior to that reported in S. angustifolia wild plants, and in comparison to cells in the suspension of S. angustifolia in MS medium with nitrate restriction this was 263-fold higher when cultured in flasks and 5-fold higher in a stirred-tank type bioreactor. The SaTR N7.2, SaTR N5.1, SaTR N7.1, and SaTR N15.1 lines excreted sphaeralcic acid into the culture medium at similar levels. Genetic transformation of hairy roots was confirmed by amplifying a 490 bp fragment of the rolC gene. S. angustifolia hairy root cultures producers of scopoletin and sphaeralcic acid can be stressed by nitrate reduction and/or copper increased to stimulate scopoletin and sphaeralcic acid production.

scholarly journals Genetically transformed hairy roots producing agropine induced on Trigonella foenum-graecum L. plant by Agrobacterium rhizogenes 1601.

2013 ◽  
Vol 7 (1) ◽  
pp. 91-98
Author(s):  
M. M. Al-Mahdawe ◽  
M. K. Al-Mallah ◽  
A. O. Al-Attrakchii
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Roots ◽  
Root Formation ◽  
Paper Electrophoresis ◽  
Transformed Roots ◽  
Fresh Weight ◽  
Trigonella Foenum Graecum ◽  
Transformed Hairy Roots ◽  
Long Time ◽  
Trigonella Foenum Graecum L

Inoculation of leaves and stems explants excised from field-grown Trigonella foenum-graecum L. with engineered Agrobacterium rhizogenes 1601 inoculum of optical density 1.90, 2.06 and 1.96 led to the formation of hairy roots on these explants. The highest percent of infection with inoculum of O.D 1.90 was 20% in leaves, and was 53.3% in stems using inoculum of O.D 2.06. Infection percent was 50% in hypocotyl compared with non-inoculated explants. The results showed that inoculation of seedlings lacking roots were slow in hairy root formation and take long time compared with their formation in intact seedlings inoculated with the same inocula. Results of paper electrophoresis of these root proved the incidence of their genetic transformation. Moreover, these transformed roots grow happily in liquid WP medium with an average fresh weight 5.9g after four weeks of culture, whereas fresh weight of normal roots was 1.02g under the same conditions.

Transformed hairy roots of Mesembryantemum crystallinum: gene expression patterns upon salt stress

1994 ◽  
Vol 90 (4) ◽  
pp. 708-714 ◽  
Author(s):  
Peter Andolfatto ◽  
Angela Bornhouser ◽  
Hans J. Bohnert ◽  
John C. Thomas
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Hairy Roots ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Transformed Hairy Roots
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