scholarly journals Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1486 ◽  
Author(s):  
Zhiyong Zhan ◽  
Yicun Chen ◽  
Jay Shockey ◽  
Xiaojiao Han ◽  
Yangdong Wang
Keyword(s):  
Proteomic Analysis ◽  
Developmental Stages ◽  
Tung Tree ◽  
Vernicia Fordii

scholarly journals The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

2019 ◽  
Author(s):  
Lin Zhang ◽  
Meilan Liu ◽  
Hongxu Long ◽  
Wei Dong ◽  
Asher Pasha ◽  
...  
Keyword(s):  
Single Molecule ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Developmental Stages ◽  
Synergistic Effects ◽  
Tung Tree ◽  
Sequencing Data ◽  
Tung Oil ◽  
Vernicia Fordii ◽  
Oil Biosynthesis ◽  
Long Reads

AbstractTung tree (Vernicia fordii) is an economically important woody oil plant that produces tung oil containing a high proportion of eleostearic acid (∼80%). Here we report a high-quality, chromosome-scale tung tree genome sequence of 1.12 Gb with 28,422 predicted genes and over 73% repeat sequences. Tung tree genome was assembled by combining Illumina short reads, PacBio single-molecule real-time long reads and Hi-C sequencing data. Insertion time analysis revealed that the repeat-driven tung tree genome expansion might be due to long standing long terminal repeat (LTR) retrotransposon bursts and lack of efficient DNA deletion mechanisms. An electronic fluorescent pictographic (eFP) browser was generated based on genomic and RNA-seq data from 17 various tissues and developmental stages. We identified 88 nucleotide-binding site (NBS)-encoding resistance genes, of which 17 genes may help the tung tree resist the Fusarium wilt shortly after infection. A total of 651 oil-related genes were identified and 88 of them were predicted to be directly involved in tung oil biosynthesis. The fewer phosphoenolpyruvate carboxykinase (PEPC) genes, and synergistic effects between transcription factors and oil biosynthesis-related genes may contribute to high oil content in tung seeds. The tung tree genome should provide valuable resources for molecular breeding and genetic improvement.

scholarly journals Integrated transcriptomic and proteomic analysis reveals the complex molecular mechanisms underlying stone cell formation in Korla pear

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aisajan Mamat ◽  
Kuerban Tusong ◽  
Juan Xu ◽  
Peng Yan ◽  
Chuang Mei ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Cell Formation ◽  
Parenchyma Cell ◽  
Lignin Biosynthesis ◽  
Cell Content ◽  
Stone Cell ◽  
Fruit Samples

AbstractKorla pear (Pyrus sinkiangensis Yü) is a landrace selected from a hybrid pear species in the Xinjiang Autonomous Region in China. In recent years, pericarp roughening has been one of the major factors that adversely affects fruit quality. Compared with regular fruits, rough-skin fruits have a greater stone cell content. Stone cells compose sclerenchyma tissue that is formed by secondary thickening of parenchyma cell walls. In this work, we determined the main components of stone cells by isolating them from the pulp of rough-skin fruits at the ripening stage. Stone cell staining and apoptosis detection were then performed on fruit samples that were collected at three different developmental stages (20, 50 and 80 days after flowering (DAF)) representing the prime, late and stationary stages of stone cell differentiation, respectively. The same batches of samples were used for parallel transcriptomic and proteomic analysis to identify candidate genes and proteins that are related to SCW biogenesis in Korla pear fruits. The results showed that stone cells are mainly composed of cellulose (52%), hemicellulose (23%), lignin (20%) and a small amount of polysaccharides (3%). The periods of stone cell differentiation and cell apoptosis were synchronous and primarily occurred from 0 to 50 DAF. The stone cell components increased abundantly at 20 DAF but then decreased gradually. A total of 24,268 differentially expressed genes (DEGs) and 1011 differentially accumulated proteins (DAPs) were identified from the transcriptomic and proteomic data, respectively. We screened the DEGs and DAPs that were enriched in SCW-related pathways, including those associated with lignin biosynthesis (94 DEGs and 31 DAPs), cellulose and xylan biosynthesis (46 DEGs and 18 DAPs), S-adenosylmethionine (SAM) metabolic processes (10 DEGs and 3 DAPs), apoplastic ROS production (16 DEGs and 2 DAPs), and cell death (14 DEGs and 6 DAPs). Among the identified DEGs and DAPs, 63 significantly changed at both the transcript and protein levels during the experimental periods. In addition, the majority of these identified genes and proteins were expressed the most at the prime stage of stone cell differentiation, but their levels gradually decreased at the later stages.

Development of Microsatellite Markers in Tung Tree (Vernicia fordii) Using Cassava Genomic Sequences

2014 ◽  
Vol 33 (4) ◽  
pp. 893-904 ◽  
Author(s):  
Lingling Zhang ◽  
Mingcheng Luo ◽  
Frank M. You ◽  
Eviatar Nevo ◽  
Shiyou Lu ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genomic Sequences ◽  
Tung Tree ◽  
Vernicia Fordii

Proteomic analysis and immunodetection of antigens from early developmental stages of Trichinella spiralis

2016 ◽  
Vol 231 ◽  
pp. 22-31 ◽  
Author(s):  
Rosa Ma. Bermúdez-Cruz ◽  
R. Fonseca–Liñán ◽  
Lucia Elhy Grijalva-Contreras ◽  
Guillermo Mendoza-Hernández ◽  
M. Guadalupe Ortega-Pierres
Keyword(s):  
Proteomic Analysis ◽  
Developmental Stages ◽  
Trichinella Spiralis ◽  
Early Developmental Stages ◽  
Early Developmental

scholarly journals Proteomic Analysis of Cell Walls of Two Developmental Stages of Alfalfa Stems

2012 ◽  
Vol 3 ◽  
Author(s):  
Julian C. Verdonk ◽  
Ronald D. Hatfield ◽  
Michael L. Sullivan
Keyword(s):  
Proteomic Analysis ◽  
Cell Walls ◽  
Developmental Stages

scholarly journals Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e88409 ◽  
Author(s):  
Heping Cao ◽  
Lin Zhang ◽  
Xiaofeng Tan ◽  
Hongxu Long ◽  
Jay M. Shockey
Keyword(s):  
Differential Expression ◽  
Tung Tree ◽  
Vernicia Fordii

Differential proteomic analysis of developmental stages of Acca sellowiana somatic embryos

2009 ◽  
Vol 31 (3) ◽  
pp. 501-514 ◽  
Author(s):  
Gabriela Claudia Cangahuala-Inocente ◽  
Andrea Villarino ◽  
Daniela Seixas ◽  
Eliane Dumas-Gaudot ◽  
Hernán Terenzi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Somatic Embryos ◽  
Developmental Stages ◽  
Acca Sellowiana
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