scholarly journals Construction of an Immobilized Thermophilic Esterase on Epoxy Support for Poly(ε-caprolactone) Synthesis

Molecules ◽  
2016 ◽  
Vol 21 (6) ◽  
pp. 796 ◽  
Author(s):  
Hui Ren ◽  
Zhen Xing ◽  
Jiebing Yang ◽  
Wei Jiang ◽  
Gang Zhang ◽  
...  
Keyword(s):  
Thermophilic Esterase

scholarly journals Alicyclobacillus acidocaldarius Thermophilic Esterase EST2's Activity in Milk and Cheese Models

2006 ◽  
Vol 72 (5) ◽  
pp. 3191-3197 ◽  
Author(s):  
Luigi Mandrich ◽  
Giuseppe Manco ◽  
Mos� Rossi ◽  
Esther Floris ◽  
Tanja Jansen-van den Bosch ◽  
...  
Keyword(s):  
Short Chain Fatty Acid ◽  
Hydrolytic Activity ◽  
Spectrophotometric Assay ◽  
Fatty Acid Esters ◽  
Optimal Conditions ◽  
Ester Synthesis ◽  
Thermophilic Enzyme ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius ◽  
Acid Esters

ABSTRACT The aim of this work was to investigate the behavior of thermophilic esterase EST2 from Alicyclobacillus acidocaldarius in milk and cheese models. The pure enzyme was used to compare the EST2 hydrolytic activity to the activity of endogenous esterase EstA from Lactococcus lactis. The results indicate that EST2 exhibits 30-fold-higher esterase activity than EstA. As EstA has thioesterase activity, EST2 was assayed for this activity under the optimal conditions determined for EstA (namely, 30�C and pH 7.5). Although it is a thermophilic enzyme, EST2 exhibited eightfold-higher thioesterase activity than EstA with S-methyl thiobutanoate. The abilities of EST2 and EstA to synthesize short-chain fatty acid esters were compared. Two methods were developed to do this. In the first method a spectrophotometric assay was used to monitor the synthesis of esters by the pure enzymes using p-nitrophenol as the alcohol substrate. The synthetic activities were also evaluated under conditions that mimicked those present in milk and/or cheese. The second method involved evaluation of the synthetic abilities of the enzymes when they were directly added to a model cheese matrix. Substantial ester synthesis by EST2 was observed under both conditions. Finally, esterase and thioesterase activities were evaluated in milk using the purified EST2 enzyme and in the model cheese matrix using a strain of L. lactis NZ9000 harboring the EST2 gene and thus overproducing EST2. Both the esterase and thioesterase activities measured in milk and in the cheese matrix were much greater than the activities of the controls.

scholarly journals Biocatalytic Synthesis of Poly(δ-Valerolactone) Using a Thermophilic Esterase from Archaeoglobus fulgidus as Catalyst

2012 ◽  
Vol 13 (12) ◽  
pp. 12232-12241 ◽  
Author(s):  
Hong Cao ◽  
Haobo Han ◽  
Guangquan Li ◽  
Jiebing Yang ◽  
Lingfei Zhang ◽  
...  
Keyword(s):  
Archaeoglobus Fulgidus ◽  
Thermophilic Esterase

Enlarging the substrate portfolio of the thermophilic esterase EST2 from Alicyclobacillus acidocaldarius

Extremophiles ◽  
2015 ◽  
Vol 19 (5) ◽  
pp. 1001-1011 ◽  
Author(s):  
Angela Pennacchio ◽  
Luigi Mandrich ◽  
Giuseppe Manco ◽  
Antonio Trincone
Keyword(s):  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius

scholarly journals The addition of the thermophilic esterase EST2 influences the fatty acids and volatile compound profiles of semi hard cheeses

2019 ◽  
Vol 39 (3) ◽  
pp. 711-720 ◽  
Author(s):  
Valentina DE LUCA ◽  
Maria Cristina PEROTTI ◽  
Irma Veronica WOLF ◽  
Carlos Alberto MEINARDI ◽  
Luigi MANDRICH
Keyword(s):  
Fatty Acids ◽  
Volatile Compound ◽  
Thermophilic Esterase

Irreversible inhibition of the thermophilic esterase EST2 from Alicyclobacillus acidocaldarius

Extremophiles ◽  
2008 ◽  
Vol 12 (5) ◽  
pp. 719-728 ◽  
Author(s):  
Ferdinando Febbraio ◽  
Sandro Esposito D’Andrea ◽  
Luigi Mandrich ◽  
Luigia Merone ◽  
Mosè Rossi ◽  
...  
Keyword(s):  
Irreversible Inhibition ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius

Biochemical characterization and mutational improvement of a thermophilic esterase from Sulfolobus solfataricus P2

2010 ◽  
Vol 32 (8) ◽  
pp. 1151-1157 ◽  
Author(s):  
Yu-Shuan Shang ◽  
Xian-En Zhang ◽  
Xu-De Wang ◽  
Yong-Chao Guo ◽  
Zhi-Ping Zhang ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Sulfolobus Solfataricus ◽  
Thermophilic Esterase

Functional and structural features of the oxyanion hole in a thermophilic esterase from Alicyclobacillus acidocaldarius

2007 ◽  
Vol 71 (4) ◽  
pp. 1721-1731 ◽  
Author(s):  
Luigi Mandrich ◽  
Valeria Menchise ◽  
Vincenzo Alterio ◽  
Giuseppina De Simone ◽  
Carlo Pedone ◽  
...  
Keyword(s):  
Structural Features ◽  
Oxyanion Hole ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius
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