scholarly journals Expression Profiling of Glucosinolate Biosynthetic Genes in Brassica oleracea L. var. capitata Inbred Lines Reveals Their Association with Glucosinolate Content

Molecules ◽  
2016 ◽  
Vol 21 (6) ◽  
pp. 787 ◽  
Author(s):  
Arif Robin ◽  
Go-Eun Yi ◽  
Rawnak Laila ◽  
Kiwoung Yang ◽  
Jong-In Park ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Expression Profiling ◽  
Inbred Lines ◽  
Glucosinolate Content ◽  
Biosynthetic Genes ◽  
Brassica Oleracea L

Quantification of glucosinolates, anthocyanins, free amino acids, and vitamin C in inbred lines of cabbage (Brassica oleracea L.)

2014 ◽  
Vol 145 ◽  
pp. 77-85 ◽  
Author(s):  
Suhyoung Park ◽  
Mariadhas Valan Arasu ◽  
Min-Ki Lee ◽  
Jin-Hyuk Chun ◽  
Jeong Min Seo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Vitamin C ◽  
Brassica Oleracea ◽  
Free Amino Acids ◽  
Free Amino ◽  
Inbred Lines ◽  
Brassica Oleracea L

scholarly journals Growth, yield and shelf-life of isopentenyltransferase (ipt)-gene transformed broccoli

2009 ◽  
Vol 89 (4) ◽  
pp. 701-711 ◽  
Author(s):  
L -F Chan ◽  
L. -F.O. Chen ◽  
H -Y Lu ◽  
C -H Lin ◽  
H -C Huang ◽  
...  
Keyword(s):  
Shelf Life ◽  
Brassica Oleracea ◽  
Inbred Line ◽  
Growth Parameters ◽  
Growth Yield ◽  
Single Copy ◽  
Inbred Lines ◽  
Yield Potential ◽  
Transgenic Lines ◽  
Brassica Oleracea L

Loss of chlorophyll leading to floret yellowing limits the post-harvest lifespan of broccoli (Brassica oleracea L. var. italica Plenck). Cytokinins are known to delay floral yellowing of plants. A transgene construct pSG766A, which results in the expression of isopentenyltransferase (ipt), the key enzyme for cytokinin synthesis, has been developed in broccoli. Expression of the ipt transgene is triggered by the senescence-associated gene promoter (SAG-13). Three selfed T5 lines of ipt transformed broccoli (lines 101, 102 and 103) have been obtained through selection for single copy insertion, acceptable horticultural traits and transgene ipt activity. These three transgenic inbred lines were evaluated in the field during 2004-2007 to determine their growth, yield and shelf-life after harvest, relative to a non-transgenic inbred line (104) and the parental variety Green King. For most of the vegetative growth parameters measured, year-to-year variability exceeded line-to-line variability. Inbreeding had little impact on the appearance or yield potential of the broccoli lines. Head yields of the transgenic inbred lines 102 and 103 were comparable to the parental variety Green King, but were significantly higher than the non-transgenic inbred line 104, as lines 102 and 103 produced more plants with heavier flower heads. Cytokinin content in the form of isopentenyladenosine was relatively higher in the transgenic lines than in the two non-transgenic controls. When flower heads were stored at 25 ± 2°C, the period required to cause 50% floret yellowing was 7.5 and 8.5 d for the transgenic lines 102 and 103, respectively, compared with 5.6 d for the non-transgenic line 104, and 5.1 d for the parental variety Green King. This study showed that the ipt-transformed inbred lines of broccoli combined acceptable appearance and yields with enhanced shelf-life.Key words: Brassica oleracea L. var. italica Plenck, transgenic broccoli, isopentenyltransferase gene, genetic characterization, shelf-life

scholarly journals Analysis and metabolite profiling of glucosinolates, anthocyanins and free amino acids in inbred lines of green and red cabbage (Brassica oleracea L.)

LWT ◽  
2014 ◽  
Vol 58 (1) ◽  
pp. 203-213 ◽  
Author(s):  
Suhyoung Park ◽  
Mariadhas Valan Arasu ◽  
Min-Ki Lee ◽  
Jin-Hyuk Chun ◽  
Jeong Min Seo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Brassica Oleracea ◽  
Free Amino Acids ◽  
Metabolite Profiling ◽  
Free Amino ◽  
Inbred Lines ◽  
Red Cabbage ◽  
Brassica Oleracea L

INHERITANCE OF GOITRIN, VOLATILE ISOTHIOCYANATES AND THIOCYANATE ION IN CABBAGE (Brassica oleracea L. ssp. capitata L.)

1987 ◽  
Vol 67 (2) ◽  
pp. 567-574 ◽  
Author(s):  
M. S. CHIANG ◽  
R. CRETE ◽  
CALVIN CHONG ◽  
G. CHEVRIER
Keyword(s):  
Brassica Oleracea ◽  
Maternal Effect ◽  
Inbred Lines ◽  
Broad Sense ◽  
The Other ◽  
Broad Sense Heritability ◽  
Thiocyanate Ion ◽  
Brassica Oleracea L ◽  
Six Genes

The inheritance of the three glucosinolate components, goitrin, volatile isothiocyanates and thiocyanate ion, was studied in cabbage (Brassica oleracea L. ssp. capitata L.) plants obtained from various crosses between two moderate inbred lines, one selected for low, the other for high goitrin content. While all three glucosinolate components showed a strong heterosis towards lower concentrations, maternal effect in inheritance was observed for goitrin only. Inheritance of both goitrin and volatile isothiocyanates was controlled by four to six genes, whereas three to five genes were involved in thiocyanate ion. Estimates of broad-sense heritability were 35% for goitrin, 39% for volatile isothiocyanates and 80% for thiocyanate ion. Evidence suggests that some of the genes controlling inheritance of goitrin and volatile isothiocyanates are linked.Key words: Glucosinolate, cabbage, glucosinolate inheritance, Brassica

scholarly journals Glucosinolate Induction and Resistance to the Cabbage Moth, Mamestra brassicae, Differs among Kale Genotypes with High and Low Content of Sinigrin and Glucobrassicin

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1951
Author(s):  
Francisco Rubén Badenes-Pérez ◽  
María Elena Cartea
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Brassica Oleracea ◽  
Insect Pest ◽  
Glucosinolate Content ◽  
Mamestra Brassicae ◽  
Generalist Insect ◽  
Lepidoptera Noctuidae ◽  
Cabbage Moth ◽  
Brassica Oleracea L

The cabbage moth, Mamestra brassicae L. (Lepidoptera: Noctuidae), is a generalist insect pest of cruciferous crops. We tested glucosinolate induction by jasmonic acid (JA) and salicylic acid (SA), and by these phytohormones combined with feeding by M. brassicae larvae in four genotypes of kale, Brassica oleracea L. var. acephala (Brassicaceae). The genotypes tested had high glucobrassicin (genotype HGBS), low glucobrassicin (genotype LGBS), high sinigrin (genotype HSIN), and low sinigrin content (genotype LSIN). Application of JA increased indolic and total glucosinolate content in all kale genotypes 1, 3, and 9 days after treatment. For SA-treated plants, glucosinolate induction varied depending on the number of days after treatment and the genotype. Overall, herbivory by M. brassicae accentuated and attenuated the effects of JA and SA, respectively, on plant glucosinolate content. Larvae of M. brassicae gained less weight on leaves from plants treated with JA compared to leaves from control plants and plants treated with SA. In bioassays with leaf discs, a significant reduction of defoliation only occurred in JA-treated plants of the HSIN genotype. This research shows that previous herbivory alters the susceptibility of kale to M. brassicae and that induction of glucosinolates varies among kale genotypes differing in their glucosinolate content.

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