scholarly journals Tailoring the Spacer Arm for Covalent Immobilization of Candida antarctica Lipase B—Thermal Stabilization by Bisepoxide-Activated Aminoalkyl Resins in Continuous-Flow Reactors

Molecules ◽  
2016 ◽  
Vol 21 (6) ◽  
pp. 767 ◽  
Author(s):  
Emese Abaházi ◽  
Dávid Lestál ◽  
Zoltán Boros ◽  
László Poppe
Keyword(s):  
Continuous Flow ◽  
Thermal Stabilization ◽  
Candida Antarctica ◽  
Covalent Immobilization ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Flow Reactors ◽  
Spacer Arm ◽  
Continuous Flow Reactors

Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides

2009 ◽  
Vol 62 (8) ◽  
pp. 799 ◽  
Author(s):  
Nemanja Miletić ◽  
Katja Loos
Keyword(s):  
Thermal Stability ◽  
Kinetic Resolution ◽  
Structural Rearrangement ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Cross Linking ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Chemically Modified ◽  
Spacer Arm

Candida antarctica lipase B, Cal-B, was (i) chemically modified with various epoxides, or (ii) cross-linked with various diepoxides in order to improve enzyme activity and thermal stability. Changed enzyme structure was confirmed by kinetic resolution of p-nitrophenol acetate with methanol and determination of the kinetic parameters. Thermal stability measurements were carried out for each Cal-B derivative, proving that Cal-B modified with 1,2-epoxypropane and 1,2-epoxypentane, and cross-linked with 1,2,7,8-diepoxyoctane and 1,2,9,10-diepoxydecane showed higher stability than soluble enzyme. The influence of the length of the epoxide arm in the modification process, as well as the length of the spacer arm in the cross-linking process, on the final enzyme preparation properties was investigated. The amount of epoxides and diepoxides used for structural rearrangement played an important role in the derivatives’ characteristics. The influence of the precipitant used and the presence of additives, such as surfactant or crown-ether, during modification and cross-linking processes was also revealed.

Pore-expanded SBA-15 for the immobilization of a recombinant Candida antarctica lipase B: Application in esterification and hydrolysis as model reactions

2018 ◽  
Vol 129 ◽  
pp. 12-24 ◽  
Author(s):  
Nathalia Saraiva Rios ◽  
Maisa Pessoa Pinheiro ◽  
Magno Luís Bezerra Lima ◽  
Denise Maria Guimarães Freire ◽  
Ivanildo José da Silva ◽  
...  
Keyword(s):  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Model Reactions
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