scholarly journals Development of Singlet Oxygen Luminescence Kinetics during the Photodynamic Inactivation of Green Algae

Molecules ◽  
2016 ◽  
Vol 21 (4) ◽  
pp. 485 ◽  
Author(s):  
Tobias Bornhütter ◽  
Judith Pohl ◽  
Christian Fischer ◽  
Irena Saltsman ◽  
Atif Mahammed ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Green Algae ◽  
Photodynamic Inactivation ◽  
Luminescence Kinetics

On the Mechanism of the Acridine Orange Sensitized Photodynamic Inactivation of Lysozyme

1976 ◽  
Vol 31 (1-2) ◽  
pp. 29-39 ◽  
Author(s):  
Peter Rosenkranz ◽  
I. Basic Kinetics ◽  
Hartmut Schmidt
Keyword(s):  
Singlet Oxygen ◽  
Acridine Orange ◽  
Ph Value ◽  
Photodynamic Inactivation ◽  
Rate Process ◽  
First Order ◽  
Quenching Process ◽  
Pseudo First Order ◽  
Kinetics Of ◽  
Excited Oxygen

The kinetics of the photodynamic desactivation of lysozyme in presence of acridine orange as the sensitizer have been investigated in detail varying oxygen, protein, dye concentration, ionic strength and pH value. The kinetics can be approximately described as an over all pseudo-first- order rate process. Changing the solvent from water to D2O or by quenching experiments in pres­ence of azide ions it could be shown that the desactivation of lysozyme is caused exclusively by singlet oxygen. The excited oxygen occurs via the triplet state of the dye with a rate constant considerably lower than that to be expected for a diffusionally controlled reaction. Singlet oxygen reacts chemically (desactivation, k=2.9 × 107 ᴍ-1 sec-1) and physically (quenching process, k = 4.1 × 108 ᴍ-1sec-1) with the enzyme. The kinetical analysis shows that additional chemical reactions between singlet oxygen and lysozyme would have only little influence on the kinetics of the desactivation as long as their products would be enzymatically active and their kinetical constants would be less than about 1 × 108 ᴍ-1 sec-1.

A maltoheptaose-decorated BODIPY photosensitizer for photodynamic inactivation of Gram-positive bacteria

2019 ◽  
Vol 43 (38) ◽  
pp. 15057-15065 ◽  
Author(s):  
Jie Hao ◽  
Zhi Song Lu ◽  
Chang Ming Li ◽  
Li Qun Xu
Keyword(s):  
Singlet Oxygen ◽  
Photodynamic Inactivation ◽  
Gram Positive ◽  
Oxygen Generation ◽  
Gram Positive Bacteria ◽  
Singlet Oxygen Generation

A maltoheptaose-decorated BODIPY with high singlet oxygen generation efficacy was synthesized for photodynamic inactivation of Gram-positive bacteria in planktonic forms and biofilms.

Singlet oxygen production by combining erythrosine and halogen light for photodynamic inactivation of Streptococcus mutans

2016 ◽  
Vol 15 ◽  
pp. 127-132 ◽  
Author(s):  
Camila Fracalossi ◽  
Juliana Yuri Nagata ◽  
Diogo Silva Pellosi ◽  
Raquel Sano Suga Terada ◽  
Noboru Hioka ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Streptococcus Mutans ◽  
Photodynamic Inactivation ◽  
Oxygen Production ◽  
Halogen Light

scholarly journals Singlet oxygen luminescence kinetics in a heterogeneous environment – identification of the photosensitizer localization in small unilamellar vesicles

2015 ◽  
Vol 14 (2) ◽  
pp. 329-334 ◽  
Author(s):  
S. Hackbarth ◽  
B. Röder
Keyword(s):  
Singlet Oxygen ◽  
Heterogeneous Environment ◽  
Unilamellar Vesicles ◽  
Luminescence Kinetics ◽  
Environment Identification ◽  
Small Unilamellar Vesicles

Singlet oxygen luminescence kinetics in microheterogeneous environment reflect the localization of the photosensitizer.

scholarly journals Potentiation of photoinactivation of Gram-positive and Gram-negative bacteria mediated by six phenothiazinium dyes by addition of azide ion

2014 ◽  
Vol 13 (11) ◽  
pp. 1541-1548 ◽  
Author(s):  
Kamola R. Kasimova ◽  
Magesh Sadasivam ◽  
Giacomo Landi ◽  
Tadeusz Sarna ◽  
Michael R. Hamblin
Keyword(s):  
Singlet Oxygen ◽  
Hydroxyl Radicals ◽  
Gram Negative Bacteria ◽  
Photodynamic Inactivation ◽  
Gram Positive ◽  
Gram Negative ◽  
Azide Ion

Antimicrobial photodynamic inactivation (APDI) using six different phenothiazinium dyes is mediated by singlet oxygen (quenched by azide) and hydroxyl radicals (potentiated by azide) depending on Gram-classification of the bacteria and whether the dye is washed from the cells.

scholarly journals Photodynamic Inactivation of Legionella Pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness

2021 ◽  
Vol 22 (16) ◽  
pp. 9095
Author(s):  
Martina Mušković ◽  
Iva Ćavar ◽  
Andrija Lesar ◽  
Martin Lončarić ◽  
Nela Malatesti ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Legionella Pneumophila ◽  
Biofilm Formation ◽  
Water Hardness ◽  
Antimicrobial Effect ◽  
Growth Stages ◽  
Photodynamic Inactivation ◽  
Waterborne Diseases ◽  
Biofilm Growth ◽  
Biofilm Destruction

The bacterium Legionella pneumophila is still one of the probable causes of waterborne diseases, causing serious respiratory illnesses. In the aquatic systems, L. pneumophila exists inside free-living amoebae or can form biofilms. Currently developed disinfection methods are not sufficient for complete eradication of L. pneumophila biofilms in water systems of interest. Photodynamic inactivation (PDI) is a method that results in an antimicrobial effect by using a combination of light and a photosensitizer (PS). In this work, the effect of PDI in waters of natural origin and of different hardness, as a treatment against L. pneumophila biofilm, was investigated. Three cationic tripyridylporphyrins, which were previously described as efficient agents against L. pneumophila alone, were used as PSs. We studied how differences in water hardness affect the PSs’ stability, the production of singlet oxygen, and the PDI activity on L. pneumophila adhesion and biofilm formation and in biofilm destruction. Amphiphilic porphyrin showed a stronger tendency for aggregation in hard and soft water, but its production of singlet oxygen was higher in comparison to tri- and tetracationic hydrophilic porphyrins that were stable in all water samples. All three studied porphyrins were shown to be effective as PDI agents against the adhesion of the L. pneumophila to polystyrene, against biofilm formation, and in the destruction of the formed biofilm, in their micromolar concentrations. However, a higher number of dissolved ions, i.e., water hardness, generally reduced somewhat the PDI activity of all the porphyrins at all tested biofilm growth stages.

Fluorescent proteins as singlet oxygen photosensitizers: mechanistic studies in photodynamic inactivation of bacteria

2013 ◽  
Author(s):  
Rubén Ruiz-González ◽  
John H. White ◽  
Aitziber L. Cortajarena ◽  
Montserrat Agut ◽  
Santi Nonell ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Fluorescent Proteins ◽  
Photodynamic Inactivation ◽  
Mechanistic Studies ◽  
Inactivation Of Bacteria

scholarly journals The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria

2007 ◽  
Vol 104 (17) ◽  
pp. 7223-7228 ◽  
Author(s):  
T. Maisch ◽  
J. Baier ◽  
B. Franz ◽  
M. Maier ◽  
M. Landthaler ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Oxygen Concentration ◽  
Photodynamic Inactivation ◽  
Inactivation Of Bacteria
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