scholarly journals Inhibition of Tapeworm Thioredoxin and Glutathione Pathways by an Oxadiazole N-Oxide Leads to Reduced Mesocestoides vogae Infection Burden in Mice

Molecules ◽  
2015 ◽  
Vol 20 (7) ◽  
pp. 11793-11807 ◽  
Author(s):  
Vivian Pasquet ◽  
Hugo Bisio ◽  
Gloria López ◽  
Laura Romanelli-Cedrez ◽  
Mariana Bonilla ◽  
...  
Keyword(s):  
Mesocestoides Vogae

First description of peritoneal and pleural metacestodosis caused by Mesocestoides vogae in a European wild cat (Felis silvestris silvestris)

2021 ◽  
Author(s):  
Magda Sindičić ◽  
Andrea Gudan Kurilj ◽  
Franjo Martinković ◽  
Miljenko Bujanić ◽  
Maja Lukač ◽  
...  
Keyword(s):  
Felis Silvestris ◽  
Felis Silvestris Silvestris ◽  
Mesocestoides Vogae

scholarly journals Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Terézia Mačak Kubašková ◽  
Dagmar Mudroňová ◽  
Miroslava Vargová ◽  
Katarína Reiterová ◽  
Gabriela Hrčková
Keyword(s):  
T Cells ◽  
Cell Population ◽  
Peritoneal Cavity ◽  
Post Infection ◽  
Initial Increase ◽  
Mhc Ii ◽  
Cell Functions ◽  
Peritoneal Cells ◽  
Immunosuppressive Cell ◽  
Mesocestoides Vogae

Abstract Background Here, Mesocestoides (M.) vogae infection in mice is proposed as a suitable experimental model for studying the immunity in the peritoneal cavity of mice. Methods To investigate the kinetics of immune parameters in M. vogae-infected mice, we detected, using flow cytometry, the expression of selected lymphoid and myeloid markers within the peritoneal cell population at day 0, 3, 6, 10, 14, 19, 25, 30 and 35 post-infection. Then, using ELISA, we analyzed the cytokine IFN-γ, TGF-β, IL-4 and IL-10 responses and the levels of anti-M. vogae IgG and IgM antibodies in the peritoneal lavage fluid. Cells isolated from the peritoneal cavity were subjected to further molecular analysis. To assess cell activation, peritoneal cells were exposed to LPS, and culture supernatants were collected and assayed for the level of cytokines and production of nitrite. Ly6C+ and Ly6G+ cells were isolated using MACS from the peritoneal cells at day 35 post-infection. Both MACS-isolated subsets were co-cultured with preactivated T cells to measure their suppressive capacity. Next, the role of parasite excretory-secretory antigens in induction of CD11b+ myeloid cells with the suppressive phenotype and the production of IL-10 was examined. Results In the peritoneal cavity an initial increase of CD11b+Gr-1+F4/80highMHC IIhigh cells, NK, NKT cells and CD8+ cytotoxic T cells was observed in the first week of infection. At day 14 post-infection, an increase in the number of myeloid CD11b+Gr-1+ cells was detected, and most of this cell population expressed low levels of F4/80 and MHC II in later stages of infection, suggesting the impairment of antigen-presenting cell functions, probably through the excretory-secretory molecules. Moreover, we confirmed that peritoneal Gr1+ cells (Ly6C+ and Ly6G+ population) are phenotypically and functionally consistent with myeloid-derived suppressor cells. Metacestode infection elicited high levels of IL-10 and upregulated STAT-3 in peritoneal cells. A higher level of IgM suggests that this isotype may be predominant and is involved in the host protection. Conclusions Mesocestoides vogae tetrathyridia induced the recruitment of immunosuppressive cell subsets, which may play a key role in the downregulation of immune response in long-term parasitic diseases, and excretory-secretory antigens seem to be the main regulatory factor.

scholarly journals Towards an Understanding of Mesocestoides vogae Fatty Acid Binding Proteins’ Roles

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e111204 ◽  
Author(s):  
Gabriela Alvite ◽  
Natalia Garrido ◽  
Alejandra Kun ◽  
Margot Paulino ◽  
Adriana Esteves
Keyword(s):  
Fatty Acid ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Mesocestoides Vogae

In vitro taurocholate-induced segmentation and clustering of Mesocestoides vogae (syn. corti) tetrathyridia (Cestoda) – inhibition by cestocidal drugs

2001 ◽  
Vol 87 (4) ◽  
pp. 281-286 ◽  
Author(s):  
Jenny Saldaña ◽  
Mónica Marín ◽  
Cecilia Fernández ◽  
Laura Domínguez
Keyword(s):  
Mesocestoides Vogae

Excretion of citrate by metacestodes of Mesocestoides vogae

1998 ◽  
Vol 76 (9) ◽  
pp. 1783-1786
Author(s):  
I. Corbin ◽  
J. Payette ◽  
B.J. Blackburn ◽  
M. Novak
Keyword(s):  
Mesocestoides Vogae

Tritiated thymidine ([3H]-TdR) and immunocytochemical tracing of cellular fate within the asexually dividing cestode Mesocestoides vogae (syn. M. corti)

Parasitology ◽  
2000 ◽  
Vol 121 (1) ◽  
pp. 105-110 ◽  
Author(s):  
A. G. SMITH ◽  
G. McKERR
Keyword(s):  
Tissue Repair ◽  
Liquid Scintillation ◽  
Tritiated Thymidine ◽  
Fold Increase ◽  
Spatial Arrangement ◽  
Entire Body ◽  
Recruitment Pattern ◽  
Cell Recruitment ◽  
Organ Systems ◽  
Mesocestoides Vogae

This report documents the presence of an active thymidine kinase (TK) system within Mesocestoides vogae tetrathyridia as quantified by tritiated thymidine ([3H]-TdR) incorporation using liquid scintillation counting. A 100-fold increase in [3H]-TdR incorporation was observed at 37 °C when compared with its incorporation at 0 °C. Thymidine's competitive analogue, BrdU, competed for sites within newly replicated DNA. Immunohistochemical trials performed here using antibodies against BrdU identified cells that have entered and passed through S-phase. Positively stained nuclei were most numerous at the anterior tip of tetrathyridia especially within the ganglia, lesser numbers of these cells occurred along the growing commissure and amongst surface tegumental cytons suggesting that stem cells do not exist in one region but are found throughout the entire body. As M. vogae has no internal organ systems the major sites for cell proliferation are those exhibiting maximal cell recruitment and undergoing tissue repair. These results show that it is possible to monitor changes in the cell recruitment pattern within this cestode. Thus use of BrdU and immunohistochemistry demonstrates how spatial arrangement and cellular reorganization can be successfully traced within M. vogae.

scholarly journals Phagocytosis in Mesocestoides vogae-induced peritoneal monocytes/macrophages via opsonin-dependent or independent pathways

2016 ◽  
Vol 53 (1) ◽  
pp. 3-13 ◽  
Author(s):  
G. Hrčková ◽  
E. Vendelova ◽  
S. Velebný
Keyword(s):  
Phagocytic Activity ◽  
Inflammatory Responses ◽  
Cell Types ◽  
Phagocytic Cells ◽  
Monocytic Cells ◽  
Latex Beads ◽  
Oral Inoculation ◽  
Intraperitoneal Infection ◽  
Mesocestoides Vogae

SummaryIntraperitoneal infection with larvae of cestode Mesocestoides vogae offers the opportunity to study dynamic changes in the proportion and functions of individual cell types under a direct influence of parasites. The phagocytic activity is one of the basic effector functions of professional phagocytes and receptor-mediated uptake is a central in implementation of inflammatory responses. Present study extends information on this issue by exploring several phagocytosis pathways in M. vogae-induced myelo-monocytic cells. In addition, we analyzed proportions of morphologically distinct phenotypes within macrophage compartments after oral inoculation of larvae to mice. In gradually elevated population of peritoneal exudate cells, monocytes/ macrophages and giant cell were dominant cell types from day 21 p.i. Phagocytic activity of these cells had biphasic behaviour for both opsonin-dependent and independent pathways, whereas uptake by multinucleated macrophages was profoundly reduced. Highly elevated proportions of activated phagocytic cells were found from day 7 to 14 p.i., regardless particle type (latex beads, HEMA, liposomes) and opsonisation. Source of opsonins used for coating of liposomes suggested higher expression of complement receptors than Fc receptors on these cells, although the uptake of non-opsonized liposomes had different kinetics and was very high by activated cells early p.i. Present data indicate that early recruited macrophages/monocytes attain pro-inflammatory functions as indicated by highly elevated phagocytosis of immunologically inert particles as well as opsonized liposomes what is down-regulated once larvae start to proliferate in the peritoneal cavity, suggesting the role of parasite-derived molecules in modulation of this key phagocytes function.

Two novel Mesocestoides vogae fatty acid binding proteins - functional and evolutionary implications

FEBS Journal ◽  
2007 ◽  
Vol 275 (1) ◽  
pp. 107-116 ◽  
Author(s):  
Gabriela Alvite ◽  
Lucía Canclini ◽  
Ileana Corvo ◽  
Adriana Esteves
Keyword(s):  
Fatty Acid ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Mesocestoides Vogae
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