scholarly journals Green Synthesis of Silver Nanoparticles using Achillea biebersteinii Flower Extract and Its Anti-Angiogenic Properties in the Rat Aortic Ring Model

Molecules ◽  
2014 ◽  
Vol 19 (4) ◽  
pp. 4624-4634 ◽  
Author(s):  
Javad Baharara ◽  
Farideh Namvar ◽  
Tayebe Ramezani ◽  
Nasrin Hosseini ◽  
Rosfarizan Mohamad
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Aortic Ring ◽  
Flower Extract ◽  
Ring Model ◽  
Achillea Biebersteinii ◽  
Rat Aortic Ring Model

Rat Aortic Ring Model to Assay Angiogenesis ex vivo

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (20) ◽  
Author(s):  
Isabelle Ernens ◽  
B�n�dicte Lenoir ◽  
Yvan Devaux ◽  
Daniel Wagner
Keyword(s):  
Ex Vivo ◽  
Aortic Ring ◽  
Ring Model ◽  
Rat Aortic Ring Model

scholarly journals Purified Anthocyanin Supplementation Improves Endothelial Function via NO-cGMP Activation in Hypercholesterolemic Individuals

2011 ◽  
Vol 57 (11) ◽  
pp. 1524-1533 ◽  
Author(s):  
Yanna Zhu ◽  
Min Xia ◽  
Yan Yang ◽  
Fengqiong Liu ◽  
Zhongxia Li ◽  
...  
Keyword(s):  
Endothelial Function ◽  
Hdl Cholesterol ◽  
Aortic Ring ◽  
Control Group ◽  
Short Term ◽  
Beneficial Effects ◽  
Ring Model ◽  
Vascular Adhesion ◽  
Rat Aortic Ring Model

BACKGROUND Anthocyanins have been shown to improve endothelial function in animal models. However, whether these compounds have similar beneficial effects in humans is largely unknown. METHODS In a short-term crossover study, 12 hypercholesterolemic individuals were given oral anthocyanins (320 mg) isolated from berries or placebo. Brachial artery flow-mediated dilation (FMD) was assessed before and after the intervention. In a long-term intervention trial (12 weeks), 150 hypercholesterolemic individuals were given anthocyanins (320 mg/day, n = 75) or placebo (n = 75), after which we measured FMD, plasma cGMP, and other serum biomarkers. Another short-term intervention was conducted in the presence of NO-cGMP inhibitors in 6 people and in a rat aortic ring model (n = 8). RESULTS Significant increases of FMD from 8.3% (0.6%) at baseline to 11.0% (0.8%) at 1 h and 10.1% (0.9%) at 2 h were observed after short-term anthocyanin consumption, concomitantly with increases of plasma anthocyanin concentrations (P < 0.05). In the study participants who received long-term anthocyanin intervention, compared with the control group, we observed significant increases in the FMD (28.4% vs 2.2%), cGMP (12.6% vs −1.2%), and HDL-cholesterol concentrations, but decreases in the serum soluble vascular adhesion molecule-1 and LDL cholesterol concentrations (P < 0.05). The changes in the cGMP and HDL cholesterol concentrations positively correlated with FMD in the anthocyanin group (P < 0.05). In the presence of NO-cGMP inhibitors, the effects of anthocyanin on endothelial function were abolished in human participants and in a rat aortic ring model. CONCLUSIONS Anthocyanin supplementation improves endothelium-dependent vasodilation in hypercholesterolemic individuals. This effect involves activation of the NO-cGMP signaling pathway, improvements in the serum lipid profile, and decreased inflammation.

scholarly journals B. anthracisedema toxin increases cAMP levels and inhibits phenylephrine-stimulated contraction in a rat aortic ring model

2013 ◽  
Vol 305 (2) ◽  
pp. H238-H250 ◽  
Author(s):  
Yan Li ◽  
Xizhong Cui ◽  
Steven B. Solomon ◽  
Kenneth Remy ◽  
Yvonne Fitz ◽  
...  
Keyword(s):  
Protective Antigen ◽  
Lethal Factor ◽  
Aortic Ring ◽  
Contractile Force ◽  
Host Cells ◽  
Sprague Dawley ◽  
Adenyl Cyclase Activity ◽  
Ring Model ◽  
Arterial Relaxation ◽  
Rat Aortic Ring Model

B. anthracis edema toxin (ET) and lethal toxin (LT) are each composed of protective antigen (PA), necessary for toxin uptake by host cells, and their respective toxic moieties, edema factor (EF) and lethal factor (LF). Although both toxins likely contribute to shock during infection, their mechanisms are unclear. To test whether ET and LT produce arterial relaxation, their effects on phenylephrine (PE)-stimulated contraction in a Sprague-Dawley rat aortic ring model were measured. Rings were prepared and connected to pressure transducers. Their viability was confirmed, and peak contraction with 60 mM KCl was determined. Compared with PA pretreatment (control, 60 min), ET pretreatment at concentrations similar to those noted in vivo decreased the mean (±SE) maximum contractile force (MCF; percent peak contraction) in rings generated during stimulation with increasing PE concentrations (96.2 ± 7.0 vs. 57.3 ± 9.1) and increased the estimated PE concentration producing half the MCF (EC50; 10−7M, 1.1 ± 0.3 vs. 3.7 ± 0.8, P ≤ 0.002). ET inhibition with PA-directed monoclonal antibodies, selective EF inhibition with adefovir, or removal of the ring endothelium inhibited the effects of ET on MCF and EC50( P ≤ 0.02). Consistent with its adenyl cyclase activity, ET increased tissue cAMP in endothelium-intact but not endothelium-denuded rings ( P < 0.0001 and 0.25, respectively). LT pretreatment, even in high concentrations, did not significantly decrease MCF or increase EC50(all P > 0.05). In rings precontracted with PE compared with posttreatment with PA (90 min), ET posttreatment produced progressive reductions in contractile force and increases in relaxation in endothelium-intact rings ( P < 0.0001) but not endothelium-denuded rings ( P = 0.51). Thus, ET may contribute to shock by producing arterial relaxation.

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