scholarly journals Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

Molecules ◽  
2013 ◽  
Vol 18 (8) ◽  
pp. 9999-10013 ◽  
Author(s):  
Eszter Molnár ◽  
Soujanya Kuntam ◽  
Pradeep Cingaram ◽  
Begüm Peksel ◽  
Bhavyashree Suresh ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Small Molecule ◽  
Fluorescent Dye ◽  
Live Cell ◽  
Cell Staining ◽  
Microscopy Techniques

scholarly journals Live-Cell Imaging of Endogenous mRNAs with a Small Molecule

2014 ◽  
Vol 127 (6) ◽  
pp. 1875-1878 ◽  
Author(s):  
Shin-ichi Sato ◽  
Mizuki Watanabe ◽  
Yousuke Katsuda ◽  
Asako Murata ◽  
Dan Ohtan Wang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Customizable live-cell imaging chambers for multimodal and multiplex fluorescence microscopy

2020 ◽  
Vol 98 (5) ◽  
pp. 612-623
Author(s):  
Adam Tepperman ◽  
David Jiao Zheng ◽  
Maria Abou Taka ◽  
Angela Vrieze ◽  
Austin Le Lam ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Imaging Modalities ◽  
Experimental Conditions ◽  
Microscopy Imaging ◽  
Glass Coverslip ◽  
Multiple Imaging ◽  
Microscopy Techniques

Using multiple imaging modalities while performing independent experiments in parallel can greatly enhance the throughput of microscopy-based research, but requires the provision of appropriate experimental conditions in a format that meets the optical requirements of the microscope. Although customized imaging chambers can meet these challenges, the difficulty of manufacturing custom chambers and the relatively high cost and design inflexibility of commercial chambers has limited the adoption of this approach. Herein, we demonstrate the use of 3D printing to produce inexpensive, customized, live-cell imaging chambers that are compatible with a range of imaging modalities, including super-resolution microscopy. In this approach, biocompatible plastics are used to print imaging chambers designed to meet the specific needs of an experiment, followed by adhesion of the printed chamber to a glass coverslip, producing a chamber that is impermeant to liquids and that supports the growth and imaging of cells over multiple days. This approach can also be used to produce moulds for casting microfluidic devices made of polydimethylsiloxane. The utility of these chambers is demonstrated using designs for multiplex microscopy, imaging under shear, chemotaxis, and general cellular imaging. Together, this approach represents an inexpensive yet highly customizable approach for producing imaging chambers that are compatible with modern microscopy techniques.

scholarly journals Dibenzopyridoimidazocinnolinium cations: a new family of light-up fluorescent DNA probes

2018 ◽  
Vol 5 (12) ◽  
pp. 1916-1927 ◽  
Author(s):  
Pedro Bosch ◽  
David Sucunza ◽  
Francisco Mendicuti ◽  
Alberto Domingo ◽  
Juan J. Vaquero
Keyword(s):  
Dna Binding ◽  
Fluorescence Intensity ◽  
Living Cells ◽  
Dna Probes ◽  
Live Cell ◽  
Binding Ability ◽  
Active Uptake ◽  
New Family ◽  
Cell Staining ◽  
New Compounds

A new family of weakly fluorescent azonia cations with DNA-binding ability by intercalation whose fluorescence intensity increases significantly upon DNA addition is reported. A live-cell staining cells analysis showed the capacity of these new compounds for active uptake and accumulation by living cells.

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

2018 ◽  
Vol 141 (7) ◽  
pp. 2770-2781 ◽  
Author(s):  
Lu Wang ◽  
Michelle S. Frei ◽  
Aleksandar Salim ◽  
Kai Johnsson
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy

Imaging the invisible: resolving cellular microcompartments by superresolution microscopy techniques

2013 ◽  
Vol 394 (9) ◽  
pp. 1097-1113 ◽  
Author(s):  
Michael Hensel ◽  
Jürgen Klingauf ◽  
Jacob Piehler
Keyword(s):  
Imaging Techniques ◽  
Live Cell ◽  
Temporal Organization ◽  
Membrane Microdomains ◽  
Superresolution Microscopy ◽  
Neuronal Synapses ◽  
Spatio Temporal ◽  
Microscopy Techniques ◽  
Plasma Membrane Microdomains ◽  
Diffraction Barrier

Abstract Unraveling the spatio-temporal organization of dynamic cellular microcompartments requires live cell imaging techniques capable of resolving submicroscopic structures. While the resolution of traditional far-field fluorescence imaging techniques is limited by the diffraction barrier, several fluorescence-based microscopy techniques providing sub-100 nm resolution have become available during the past decade. Here, we briefly introduce the optical principles of these techniques and compare their capabilities and limitations with respect to spatial and temporal resolution as well as live cell capabilities. Moreover, we summarize how these techniques contributed to a better understanding of plasma membrane microdomains, the dynamic nanoscale organization of neuronal synapses and the sub-compartmentation of microorganisms. Based on these applications, we highlight complementarity of these techniques and their potential to address specific challenges in the context of dynamic cellular microcompartments, as well as the perspectives to overcome current limitations of these methods.

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