scholarly journals Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives

Molecules ◽  
2013 ◽  
Vol 18 (8) ◽  
pp. 9477-9487 ◽  
Author(s):  
Diego Bou ◽  
João Lago ◽  
Carlos Figueiredo ◽  
Alisson Matsuo ◽  
Rafael Guadagnin ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Main Compound ◽  
Casearia Sylvestris

Chemical Composition, Antibacterial, Insecticidal and Anti-Oxidant Activities of Three Acantholimon Species (A. atropatanum, A. gilliatii and A. tragacanthium)

2020 ◽  
Vol 10 (3) ◽  
pp. 272-278
Author(s):  
Ardalan Pasdaran ◽  
Satyajit D. Sarker ◽  
Lutfun Nahar ◽  
Azadeh Hamedi
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Main Compound ◽  
Aerial Parts ◽  
Anti Oxidant ◽  
Gas Chromatography Mass Spectroscopy

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.

scholarly journals Chemical Composition of the Essential Oil from Chaerophyllum Temulum (Apiaceae)

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Jelena G. Stamenković ◽  
Gordana S. Stojanović ◽  
Ivana R. Radojković ◽  
Goran M. Petrović ◽  
Bojan K Zlatković
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Flowering Stage ◽  
Main Compound ◽  
Fruiting Stage ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Main Components

The present study reports the chemical composition on the essential oil obtained from fresh roots, stems, inflorescences and fruits of Chaerophyllum temulum. In all samples, except the roots, the most dominant components were sesquiterpene hydrocarbons. ( Z)-Falcarinol was the principal constituent of the root essential oils (61.7% at the flowering stage and 62.3% at the fruiting stage). The blossom oil was dominated by ( Z,E)-α-farnesene (23.4%), ( E)-β-farnesene (9.0%) and germacrene D-4-ol (9%), whereas the oil from the fruit had germacrene D-4-ol (27.6%) as its main compound, accompanied by ( Z,E)-α-farnesene (13.4%). Germacrene D was the most abundant component of the stem essential oil (38.4% at the flowering stage and 32.5% at the fruiting stage). The obtained results show that the qualitative composition of the oil depends on the part of the plant which is analyzed, while the quantitative composition of the main components depends on the growing stage of the plant.

scholarly journals Chemical composition, antimicrobial and antifungal activity of Lippia Thymoide essential oil in oral pathogens

2021 ◽  
Vol 20 ◽  
pp. e210219
Author(s):  
Tabata Resque Beckmann Carvalho ◽  
Erich Brito Tanaka ◽  
Amujacy Tavares Vilhena ◽  
Paula Cristina Rodrigues Frade ◽  
Ricardo Roberto de Souza Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Chemical Analysis ◽  
Essential Oil ◽  
Antimicrobial Agents ◽  
Fusobacterium Nucleatum ◽  
Triphenyltetrazolium Chloride ◽  
Gram Negative ◽  
Main Compound ◽  
Bacteria And Fungi

Aim: This study evaluated the chemical composition of Lippia thymoides (Lt) essential oil and its antimicrobial activity against fungal strains of Candida albicans (Ca) and Gram-negative bacteria Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn). Methods: Lt essential oil was obtained by hydrodistillation apparatus with a modified Clevenger extension. The chemical analysis was analyzed by gas phase chromatography and mass spectrometry on Shimadzu QP 2010 plus. Sample sensitivity evaluation was performed by ABHb-inoculum and culture plates were developed with triphenyltetrazolium chloride, also Fn and Pi samples analysis were in anaerobic environment and Ca sample analysis was performed in aerobic environment. The minimum inhibitory concentration (CIM) was determinated by microdilution in eppendorfs tubes. Results: The chemical analysis showed that Thymol (59,91%) is the main compound found in Lt essential oil, also other antifungal and antimicrobial agents were present γ-terpinene (8.16%), p-cymene (7.29%) and β-caryophyllene (4.49%), Thymol is a central ingredient of many medicinal plants and has a potent fungicidal, bactericidal and antioxidant activity, it has been previously shown to have anti-inflammatory activity against Periodontal Disease (PD) cause can reduces prostanoids, interleukins, leukotrienes levels in periodontium. CIM result Pi was 6.5 μg/mL, Fn was 1.5 μg/mL and Ca was 0.19 μg/mL. Conclusion: The antimicrobial activity of L. thymoides, through the compound Thymol, has been shown promising potential against gram-negative periodontopathogenic bacteria and fungi whose therapeutic arsenal is still very restricted.

Essential oil chemical composition, antimicrobial, anticancer, and antioxidant effects of Thymus convolutus Klokov in Turkey

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Selma Celen Yuceturk ◽  
Sumeyye Aydogan Turkoglu ◽  
Feray Kockar ◽  
F. Zehra Kucukbay ◽  
A. Dilek Azaz
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Umbilical Vein ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enterobacter Aerogenes ◽  
Gas Chromatography Mass Spectrometry ◽  
Human Hepatoma Cells ◽  
Main Compound ◽  
Main Component

Abstract In this study, the chemical composition, antimicrobial, antioxidant, and anticancer effects of Thymus convolutus Klokov oil and its main compound camphor were investigated. The oil was isolated from T. convolutus using hydrodistillation method, analyzed by gas chromatography/mass spectrometry (GC–MS), and 66 compounds were identified. The main component was determined as camphor at 16.6%. The antioxidant properties were identified with the DPPH (2,2′-diphenyl-1-picrylhydrazyl) radical-scavenging method and, 33.39 ± 0.25% DPPH was scavenging in 1000 μg/mL of essential oil. The strong antimicrobial activity was observed against Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, and Pseudomonas aeruginosa with MIC values of 125 μg/mL. Aspergillus flavus was more sensitive (28%) against T. convolutus essential oil than other fungi. The cytotoxic effect of oil was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Camphor was effective on human hepatoma cells (Hep3B) at concentrations of 1 mg/mL, 500, 250, and 125 μg/mL, while essential oil of T. convolutus was found to be effective at concentrations of 250 and 125 μg/mL. A reduction in cell proliferation was observed in colon carcinoma cells (HT-29) treated with 500 μg/mL camphor for 48 h. No statistically significant effect was found in Umbilical Vein Endothelial Cells (HUVEC) treated with essential oil and camphor.

scholarly journals Geographical chemical variability and processing oxidation of volatile compounds of Casearia sylvestris leaves

2021 ◽  
Vol 46 (3) ◽  
pp. 42-48
Author(s):  
Flavio Alexandre Carvalho ◽  
Fernando Bombarda Oda ◽  
Ant�nio Eduardo Miller Crotti ◽  
Eduardo Jos� Crevelin ◽  
Andr� Gonzaga Dos Santos
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Volatile Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Caryophyllene Oxide ◽  
Volatile Chemicals ◽  
Leaf Essential Oil ◽  
Casearia Sylvestris ◽  
Main Components

The Casearia sylvestris Sw. dried leaf essential oil (EO) contains sesquiterpenes as the main components. However, the volatile components in the in natura leaves remain unknown. This study compares the volatile chemicals in the in natura leaves and dried leaf EO of two C. sylvestris populations from Atlantic Forest and Cerrado. The volatile compounds were directly analysed by thermal desorption (TD) coupled to gas chromatography mass spectrometry (GC-MS); the dried leaf EO composition was determined by GC-MS. All the identified compounds were sesquiterpenes, and the major components were (E)-caryophyllene, bicyclogermacrene, ?-elemene, spathulenol, and caryophyllene oxide. In both populations, the sesquiterpene hydrocarbon content and the oxygenated sesquiterpene content respectively decreased and increased on going from the in natura leaves to the dried leaf essential oil, indicating that drying and/or hydrodistillation modified the volatile chemical composition by generating oxidation artifacts. Results suggested that (E)-caryophyllene and bicyclogermacrene may be oxidized during the process to yield caryophyllene oxide and spathulenol, respectively. The two C. sylvestris populations also differed in terms of volatile chemical composition.

scholarly journals Chemical Composition of Essential Oil of Senecio coincyi, an Endemic Species of the Central Iberian Peninsula

2011 ◽  
Vol 6 (1) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Carlos Arrabal ◽  
Felipe Martínez García ◽  
María Paz Arraiza ◽  
Silvia Guerrero García
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Iberian Peninsula ◽  
Endemic Species ◽  
Small Area ◽  
Gas Chromatography Mass Spectrometry ◽  
Unsaturated Hydrocarbons ◽  
Main Compound

The essential oil has been studied of leaves of Senecio coincyi Rouy, an endemic species of Spain restricted to a very small area of the Central Iberian Peninsula. Samples from five locations were obtained by hydrodistillation and extraction and analysed by gas chromatography-mass spectrometry. The main compound was 1-tridecene (28.1 ± 8.5%). The presence of unsaturated hydrocarbons (1-undecene, 1-dodecene and 1-tridecene) seems to indicate a chemotaxonomic relationship between Senecio coincyi and S. congestus.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil from the Seeds of Plectranthus hadiensis

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Raju Sripathi ◽  
Subban Ravi
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antibacterial Agent ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Main Compound ◽  
Ethnobotanical Uses ◽  
Total Oil

Plectranthus is a large and widespread genus of Lamiaceae family with a diversity of ethnobotanical uses. In traditional medicine, the juice of stem and leaves of Plectranthus hadiensis which is mixed with honey is taken as a remedy for diarrhea. The aim of the present study is to determine the chemical composition of the essential oil from the seed of P. hadiensis and to evaluate antimicrobial efficacy of the oil. The essential oil of the seeds from P. hadiensis is obtained by hydro-distillation and analyzed by gas chromatography coupled with mass spectrometry (GC/MS). It results in the identification of 25 compounds representing 99.3%, of the total oil. The main compound is Piperitone oxide (33.33%). Antibacterial activity of the essential oil of P. hadiensis is tested against two Gram-positive and two Gram-negative bacteria, using zone of inhibition method. The essential oils inhibit the organisms and shows the zone of inhibition in the range of 20-35mm. The essential oil can serve as an antibacterial agent.

scholarly journals Essential oil composition in natural population of Lippia origanoides (Verbenaceae) during dry and rainy seasons

2019 ◽  
Vol 67 (1) ◽  
Author(s):  
Luciana Mendes Souza ◽  
Francine Sousa Alves Fonseca ◽  
Julio Cesar Silva ◽  
Amanda Silva ◽  
Julian Silva ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Natural Population ◽  
Essential Oil Composition ◽  
Chemical Diversity ◽  
Oil Composition ◽  
Main Compound ◽  
Time Of Year ◽  
Dry And Rainy Seasons

Lippia origanoides Kunth. is a medicinal shrub native to some countries in South America, Central America and the Caribbean. This study aims to investigate the chemical composition of the essential oil and the genetic diversity of a natural population of Lippia origanoides in two seasons. Leaf samples were collected from 30 individuals in the dry and rainy seasons. The essential oil was extracted into a Clevenger apparatus and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The relative quantification of thymol and carvacrol was performed by gas chromatography coupled to a flame ionization detector (GC-FID). Multivariate analysis was used to evaluate the genetic divergence between plants. Carvacrol was the major compound found in most plants for both seasons. In the rainy season, plant 15 presented thymol as the major compound, and plants 16, 27, 28 and 29 presented camphor as the major compound. In the dry season, thymol remained the main compound of plant 15 and camphor remained the main compound of plants 16, 28 and 29; however, plant 27 presented carvacrol as the main compound in this season. After carvacrol, the compounds with the highest content were γ-terpinene, p-cymene and methyl-ether-thymol. Of the 30 plants studied, only five differed in their chemical composition, showing some degree of stability in relation to the dry and rainy seasons. There was no variation in the main compounds between the two seasons, but there was chemical diversity among the main compounds. Tocher grouping revealed five distinct groups, with group 1 including most of the plants in both dry and rainy seasons, indicating that the time of year evaluated did not significantly interfere with the essential oil composition of most plants.

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