scholarly journals Aqueous Fraction of Nephelium ramboutan-ake Rind Induces Mitochondrial-Mediated Apoptosis in HT-29 Human Colorectal Adenocarcinoma Cells

Molecules ◽  
2012 ◽  
Vol 17 (6) ◽  
pp. 6633-6657 ◽  
Author(s):  
Chim Kei Chan ◽  
Bey Hing Goh ◽  
Muhamad Noor Alfarizal Kamarudin ◽  
Habsah Abdul Kadir
Keyword(s):  
Colorectal Adenocarcinoma ◽  
Aqueous Fraction ◽  
Adenocarcinoma Cells ◽  
Ht 29

Study on the Toxicity of Calix[4]- and [6]-Arenes and their Sulfated Derivatives in Two- and Three-Dimensional Cell Cultures of Colorectal Adenocarcinoma

2021 ◽  
Vol 37 (1) ◽  
pp. 87-94
Author(s):  
S.V. Nikulin ◽  
B.Ya. Alekseev ◽  
A.N Gorbunov ◽  
I.M. Vatsuro ◽  
V.V. Kovalev ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colorectal Adenocarcinoma ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Russian Science ◽  
Adenocarcinoma Cells ◽  
Therapeutic Molecules ◽  
Derivatives Of ◽  
Ht 29 ◽  
Dimensional Cell

A comparative study of the toxicity of two unsubstituted calixarenes consisting of 4 and 6 phenolic fragments, as well as their p-sulfated derivatives, was carried out on the HT-29 colorectal adenocarcinoma cells cultured in two-dimensional (2D) and three-dimensional (3D) formats. It was shown that both unsubstituted calixarenes decrease the viability of tumor cells; calix[4]arene and calix[6]arene exhibited a cytostatic and a cytotoxic effect, respectively. Sulfated derivatives of calixarenes did not have a pronounced toxic effect on HT-29 cells. However, due to their high hydrophilicity and the ability to form adducts with various therapeutic molecules, they can be used for delivery of anticancer drugs. calixarenes, cytotoxicity, HT-29 cells, 2D cell culture, 3D cell culture The work was financially supported by the Russian Science Foundation (project no. 19-15-00397).

Effects of NVP-BEZ235 on the proliferation, migration, apoptosis and autophagy in HT-29 human colorectal adenocarcinoma cells

2016 ◽  
Vol 49 (1) ◽  
pp. 285-293 ◽  
Author(s):  
YANG YU ◽  
XIAOFENG YU ◽  
JIANXIA MA ◽  
YILI TONG ◽  
JIANFENG YAO
Keyword(s):  
Colorectal Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Ht 29

Cytotoxicity Effect and Morphological Study of Different Duku(Lansium domesticum corr.) Extract towards Human Colorectal Adenocarcinoma Cells Line (HT-29)

2017 ◽  
Vol 9 (6) ◽  
pp. 757-761 ◽  
Author(s):  
Rohin Mohd Adzim Khalili ◽  
Jumli Mimie Noratiqah ◽  
Ridzwan Norhaslinda ◽  
Abd Hadi Norhayati ◽  
Baig Atif Amin ◽  
...  
Keyword(s):  
Morphological Study ◽  
Colorectal Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Lansium Domesticum ◽  
Ht 29

Targeting apoptosis via inactivation of PI3K/Akt/mTOR signaling pathway involving NF-κB by geraniin in HT-29 human colorectal adenocarcinoma cells

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Chim Kei Chan ◽  
Liu Ying Tang ◽  
Bey Hing Goh ◽  
Habsah Abdul Kadir
Keyword(s):  
Colorectal Adenocarcinoma ◽  
Therapeutic Potential ◽  
Human Cancer ◽  
Morphological Changes ◽  
Biological Activities ◽  
Mtor Pathway ◽  
Human Cancer Cell Lines ◽  
Adenocarcinoma Cells ◽  
Underlying Mechanisms ◽  
Ht 29

Dietary phytochemicals possess a variety of biological activities which can be widely discovered in fruits, vegetables and herbs. Geraniin, an ellagitannin is commonly found in fruits and herbs which possesses multitude of health benefits including anti-diabetic, hepatoprotective, anti-inflammatory and anticancer. However, the effects of geraniin on human colorectal adenocarcinoma cells have yet to be evaluated. This study aimed to investigate the apoptotic effects of geraniin against selected human cancer cell lines and elucidate its underlying mechanisms. Geraniin exhibited the strongest cytotoxic effect on HT-29 cells as determined by MTT assay. Events of apoptosis induced by geraniin in HT-29 cells was portrayed by apoptotic morphological changes, externalization of phosphatidylserine, DNA fragmentation and dissipation of mitochondrial membrane potential. Western blot analysis was then used to investigate the mechanisms underlying observed growth inhibition. Geraniin was found to initiate p53 activation further resulting in elevation of Bak/Bcl-xL ratio and caspase-3 activation. This eventually led to HT-29 cell death via apoptosis. Additionally, exposure of geraniin on HT-29 cells found to suppress the PI3K/Akt/mTOR pathway and suppression of NF-kB. Cumulative evidences in this study suggests that geraniin inhibited colorectal adenocarcinoma cell proliferation via apoptosis induction and suppression of PI3K/Akt/mTOR pathway. Thus, these findings provide novel mechanistic insight for the therapeutic potential of geraniin in the treatment of colorectal adenocarcinoma.

scholarly journals TFF3-dependent resistance of human colorectal adenocarcinoma cells HT-29/B6 to apoptosis is mediated by miR-491-5p regulation of lncRNA PRINS

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Carlos Hanisch ◽  
Jutta Sharbati ◽  
Barbara Kutz-Lohroff ◽  
Otmar Huber ◽  
Ralf Einspanier ◽  
...  
Keyword(s):  
Colorectal Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Ht 29
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