scholarly journals Gene Cloning, Expression and Activity Analysis of Manganese Superoxide Dismutase from Two Strains of Gracilaria lemaneiformis (Gracilariaceae, Rhodophyta) under Heat Stress

Molecules ◽  
2012 ◽  
Vol 17 (4) ◽  
pp. 4522-4532 ◽  
Author(s):  
Ning Lu ◽  
Xiaonan Zang ◽  
Xuecheng Zhang ◽  
Hao Chen ◽  
Xiaoting Feng ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Heat Stress ◽  
Gene Cloning ◽  
Manganese Superoxide Dismutase ◽  
Activity Analysis ◽  
Gracilaria Lemaneiformis ◽  
Manganese Superoxide ◽  
Expression And Activity

scholarly journals Enhanced Peroxynitrite Formation Is Associated with Vascular Aging

2000 ◽  
Vol 192 (12) ◽  
pp. 1731-1744 ◽  
Author(s):  
Bernd van der Loo ◽  
Ralf Labugger ◽  
Jeremy N. Skepper ◽  
Markus Bachschmid ◽  
Juliane Kilo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Aging Process ◽  
Manganese Superoxide Dismutase ◽  
No Synthase ◽  
Vascular Aging ◽  
Enos Expression ◽  
Manganese Superoxide ◽  
O2 Production ◽  
Expression And Activity

Vascular aging is mainly characterized by endothelial dysfunction. We found decreased free nitric oxide (NO) levels in aged rat aortas, in conjunction with a sevenfold higher expression and activity of endothelial NO synthase (eNOS). This is shown to be a consequence of age-associated enhanced superoxide (·O2−) production with concomitant quenching of NO by the formation of peroxynitrite leading to nitrotyrosilation of mitochondrial manganese superoxide dismutase (MnSOD), a molecular footprint of increased peroxynitrite levels, which also increased with age. Thus, vascular aging appears to be initiated by augmented ·O2− release, trapping of vasorelaxant NO, and subsequent peroxynitrite formation, followed by the nitration and inhibition of MnSOD. Increased eNOS expression and activity is a compensatory, but eventually futile, mechanism to counter regulate the loss of NO. The ultrastructural distribution of 3-nitrotyrosyl suggests that mitochondrial dysfunction plays a major role in the vascular aging process.

scholarly journals Decreases in manganese superoxide dismutase expression and activity contribute to oxidative stress in persistent pulmonary hypertension of the newborn

2012 ◽  
Vol 303 (10) ◽  
pp. L870-L879 ◽  
Author(s):  
Adeleye J. Afolayan ◽  
Annie Eis ◽  
Ru-Jeng Teng ◽  
Ivane Bakhutashvili ◽  
Sushma Kaul ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Pulmonary Hypertension ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Pulmonary Arteries ◽  
Tyrosine Nitration ◽  
Enos Expression ◽  
Manganese Superoxide ◽  
Expression And Activity

A rapid increase in the synthesis and release of nitric oxide (NO) facilitates the pulmonary vasodilation that occurs during birth-related transition. Alteration of this transition in persistent pulmonary hypertension of the newborn (PPHN) is associated with impaired function of endothelial nitric oxide synthase (eNOS) and an increase in oxidative stress. We investigated the hypothesis that a decrease in expression and activity of mitochondrial localized manganese superoxide dismutase (MnSOD) in pulmonary artery endothelial cells (PAEC) increases oxidative stress and impairs eNOS function in PPHN. We isolated PAEC and pulmonary arteries from fetal lambs with PPHN induced by prenatal ductus arteriosus ligation or sham ligation (control). We investigated MnSOD expression and activity, tyrosine nitration of MnSOD, and mitochondrial O2− levels in PAEC from control and PPHN lambs. We introduced exogenous MnSOD via an adenoviral vector (ad-MnSOD) transduction into PAEC and pulmonary arteries of PPHN lambs. The effect of ad-MnSOD was investigated on: mitochondrial O2− levels, MnSOD and eNOS expression and activity, intracellular hydrogen peroxide (H2O2) levels, and catalase expression in PAEC. MnSOD mRNA and protein levels and activity were decreased and MnSOD tyrosine nitration was increased in PPHN-PAEC. ad-MnSOD transduction of PPHN-PAEC increased its activity two- to threefold, decreased mitochondrial O2− levels, and increased H2O2 levels and catalase expression. ad-MnSOD transduction improved eNOS expression and function and the relaxation response of PPHN pulmonary arteries. Our observations suggest that decreased MnSOD expression and activity contribute to the endothelial dysfunction observed in PPHN.

scholarly journals Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice

2015 ◽  
Vol 13 (9) ◽  
pp. 1251-1263 ◽  
Author(s):  
Takeshi Shiraya ◽  
Taiki Mori ◽  
Tatsuya Maruyama ◽  
Maiko Sasaki ◽  
Takeshi Takamatsu ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Heat Stress ◽  
Stress Tolerance ◽  
Manganese Superoxide Dismutase ◽  
Grain Filling ◽  
Manganese Superoxide ◽  
Heat Stress Tolerance ◽  
Plastid Type

Loss of manganese superoxide dismutase expression and activity in rat esophagus with external esophageal perfusion

Surgery ◽  
2007 ◽  
Vol 141 (3) ◽  
pp. 359-367 ◽  
Author(s):  
Yan Li ◽  
John M. Wo ◽  
Ruifeng R. Su ◽  
Mukunda B. Ray ◽  
Robert C.G. Martin
Keyword(s):  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide ◽  
Expression And Activity
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