scholarly journals Low Operational Stability of Enzymes in Dry Organic Solvents: Changes in the Active Site Might Affect Catalysis

Molecules ◽  
2012 ◽  
Vol 17 (2) ◽  
pp. 1870-1882 ◽  
Author(s):  
Vibha Bansal ◽  
Yamixa Delgado ◽  
Marc D. Legault ◽  
Gabriel Barletta
Keyword(s):  
Organic Solvents ◽  
Active Site ◽  
Operational Stability

scholarly journals Active-Site Motions and Polarity Enhance Catalytic Turnover of Hydrated Subtilisin Dissolved in Organic Solvents

2009 ◽  
Vol 131 (12) ◽  
pp. 4294-4300 ◽  
Author(s):  
Elton P. Hudson ◽  
Ross K. Eppler ◽  
Julianne M. Beaudoin ◽  
Jonathan S. Dordick ◽  
Jeffrey A. Reimer ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Active Site

Enhanced activity and stability in the presence of organic solvents by increased active site polarity and stabilization of a surface loop in a metalloprotease

2010 ◽  
Vol 148 (2) ◽  
pp. 231-238 ◽  
Author(s):  
A. Badoei-Dalfard ◽  
K. Khajeh ◽  
S. M. Asghari ◽  
B. Ranjbar ◽  
H. R. Karbalaei-Heidari
Keyword(s):  
Organic Solvents ◽  
Active Site ◽  
Surface Loop ◽  
Enhanced Activity

Active-site titration of serine proteases in organic solvents

1996 ◽  
Vol 50 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Pramod P. Wangikar ◽  
Douglas Carmichael ◽  
Douglas S. Clark ◽  
Jonathan S. Dordick
Keyword(s):  
Organic Solvents ◽  
Active Site ◽  
Serine Proteases

An Insight into the Active Site of Pseudomonas Fluorecens (P. cepacia) Lipase to Define the Stereochemical Demand for the Transesterification in Organic Solvents

Biocatalysis ◽  
1994 ◽  
Vol 10 (1-4) ◽  
pp. 279-288 ◽  
Author(s):  
Patrizia Ferraboschi ◽  
Silvana Casati ◽  
Simonetta De Grandi ◽  
Paride Grisenti ◽  
Enzo Santaniello
Keyword(s):  
Organic Solvents ◽  
Active Site ◽  
Insight Into

Heterogeneous Biocatalytic Processes of the Low-Temperature Synthesis of Esters: Selection of Organic Solvent

2020 ◽  
Vol 20 (4) ◽  
pp. 313-322
Author(s):  
G. A. Kovalenko ◽  
L. V. Perminova
Keyword(s):  
Low Temperature ◽  
Binary Mixtures ◽  
Organic Solvents ◽  
Diethyl Ether ◽  
Operational Stability ◽  
Thermomyces Lanuginosus ◽  
Esterification Reaction ◽  
Heptanoic Acid ◽  
Tert Butanol ◽  
Heterogeneous Biocatalysts

Processes of the low-temperature enzymatic synthesis of esters in nonaqueous media of organic solvents with participation of heterogeneous biocatalysts synthesized by the adsorption immobilization of a recombinant lipase from Thermomyces lanuginosus (Pichia pastoris producer) on macroporous carbon aerogel were investigated. The esterification reaction was conducted using individual organic solvents of different polarity, such as nonpolar ones (lоgP > 2.5) – hexadecane, hexane and toluene, and polar solvents (lоgP < 1) – diethyl ether, tert-butanol and acetone, as well as their binary mixtures of different composition, for example, a mixture of hexane with diethyl ether having the composition 1 : (1/5÷3, vol. fr.). The effect of the solvent and co-solvent nature on the properties of synthesized biocatalysts, namely the enzymatic activity and operational stability, was studied in the batch esterification of heptanoic acid (C7:0) by alcohols, particularly the polar substrates – ethanol and n-butanol, or nonpolar alcohols – n-octanol and n-hexadecanol. Activity of the synthesized biocatalysts was found to depend to a great extent on polarity of both the solvents used in the reaction and the alcohol substrates. The maximum activity of 400 EA·g–1 was observed in a medium of nonpolar and nonviscous hexane (lоgP = 2.9), whereas the minimum activity of 4 EA·g–1 – in acetone (lоgP = 0.4); esterification of heptanoic acid in the presence of tert-butanol (lоgP = 0.6) virtually did not proceed. The activity of biocatalysts was shown to depend nonmonotonically on the lоgP value of individual solvent. In the binary mixtures of organic solvents, such as hexane/diethyl ether, biocatalytic activity linearly increased with an increase in lоgPmixture; in addition, biocatalysts exhibited the maximum operational stability.

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