scholarly journals Green Synthesis and Antibacterial Effect of Silver Nanoparticles Using Vitex Negundo L.

Molecules ◽  
2011 ◽  
Vol 16 (8) ◽  
pp. 6667-6676 ◽  
Author(s):  
Mohsen Zargar ◽  
Azizah Abdul Hamid ◽  
Fatima Abu Bakar ◽  
Mariana Nor Shamsudin ◽  
Kamyar Shameli ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Effect ◽  
Vitex Negundo

scholarly journals Bio-molecule functionalized rapid one-pot green synthesis of silver nanoparticles and their efficacy toward the multidrug resistant (MDR) gut bacteria of silkworms (Bombyx mori)

RSC Advances ◽  
2020 ◽  
Vol 10 (38) ◽  
pp. 22742-22757 ◽  
Author(s):  
Sudip Some ◽  
Biraj Sarkar ◽  
Kinkar Biswas ◽  
Tushar K. Jana ◽  
Debjoy Bhattacharjya ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Antibacterial Effect ◽  
Natural Infection ◽  
Multidrug Resistant ◽  
Gut Bacteria ◽  
Silkworm Larvae ◽  
One Pot

We aimed to synthesise bio-molecule functionalized silver nanoparticles using leaf extract from mulberry variety S-1635 (Morus alba L.) and to explore its antibacterial effect on multidrug resistant gut bacteria isolated from natural infection observed from silkworm larvae.

scholarly journals Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

2016 ◽  
Author(s):  
Patcharaporn Tippayawat ◽  
Nutthakritta Phromviyo ◽  
Parichart Boueroy ◽  
Apiwat Chompoosor
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Hydrothermal Method ◽  
Plant Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Diffusion Method

Background: There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. Methods: AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results: The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological and biomedical applications. Discussion: Aloe vera extract was processed using a green and facile method. This was a hydrothermal method to reduce silver nitrate to AgNPs@AV. Varying the hydrothermal temperature provided the fine spherical shaped nanoparticles. The size of the nanomaterial was affected by its thermal preparation. The particle size of AgNPs could be tuned by varying both time and temperature. A process using a pure AG phase could go to completion in 6h at 200 oC, whereas reactions at lower temperatures required longer times. Moreover, the antibacterial effect of this hybrid nanomaterial was sufficient that it could be used to inhibit pathogenic bacteria since silver release was dependent upon its particle size. The high activity of the largest AgNPs might have resulted from a high concentration of aloe vera compounds incorporated into the AgNPs during hydrothermal synthesis.

scholarly journals Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

2016 ◽  
Author(s):  
Patcharaporn Tippayawat ◽  
Nutthakritta Phromviyo ◽  
Parichart Boueroy ◽  
Apiwat Chompoosor
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Hydrothermal Method ◽  
Plant Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Diffusion Method

Background: There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. Methods: AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results: The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological and biomedical applications. Discussion: Aloe vera extract was processed using a green and facile method. This was a hydrothermal method to reduce silver nitrate to AgNPs@AV. Varying the hydrothermal temperature provided the fine spherical shaped nanoparticles. The size of the nanomaterial was affected by its thermal preparation. The particle size of AgNPs could be tuned by varying both time and temperature. A process using a pure AG phase could go to completion in 6h at 200 oC, whereas reactions at lower temperatures required longer times. Moreover, the antibacterial effect of this hybrid nanomaterial was sufficient that it could be used to inhibit pathogenic bacteria since silver release was dependent upon its particle size. The high activity of the largest AgNPs might have resulted from a high concentration of aloe vera compounds incorporated into the AgNPs during hydrothermal synthesis.

scholarly journals Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2589 ◽  
Author(s):  
Patcharaporn Tippayawat ◽  
Nutthakritta Phromviyo ◽  
Parichart Boueroy ◽  
Apiwat Chompoosor
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Hydrothermal Method ◽  
Plant Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Diffusion Method

BackgroundThere is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity.MethodsAgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay.ResultsThe synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested onS. epidermidisandP. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100oC for 6 h and 200oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological and biomedical applications.DiscussionAloe vera extract was processed using a green and facile method. This was a hydrothermal method to reduce silver nitrate to AgNPs@AV. Varying the hydrothermal temperature provided the fine spherical shaped nanoparticles. The size of the nanomaterial was affected by its thermal preparation. The particle size of AgNPs could be tuned by varying both time and temperature. A process using a pure AG phase could go to completion in 6 h at 200oC, whereas reactions at lower temperatures required longer times. Moreover, the antibacterial effect of this hybrid nanomaterial was sufficient that it could be used to inhibit pathogenic bacteria since silver release was dependent upon its particle size. The high activity of the largest AgNPs might have resulted from a high concentration of aloe vera compounds incorporated into the AgNPs during hydrothermal synthesis.

scholarly journals Green synthesis and stabilization of silver nanoparticles using Lysimachia foenum-graecum Hance extract and their antibacterial activity

2020 ◽  
Vol 9 (1) ◽  
pp. 107-118 ◽  
Author(s):  
Widsanusan Chartarrayawadee ◽  
Phattaraporn Charoensin ◽  
Juthaporn Saenma ◽  
Thearum Rin ◽  
Phichaya Khamai ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Colloidal Solutions ◽  
Stabilizing Agents ◽  
Long Term Stability ◽  
Gram Positive Bacteria ◽  
Steric Stability

AbstractThe Lysimachia foenumgraecum Hance extract (LHE) was used for silver nanoparticles (AgNPs) synthesis. In this study, the herbal plant of Lysimachia foenumgraecum Hance (LH) was extracted with deionized water and we are the first to successfully use LHE as reducing and stabilizing agents for the green synthesis of AgNPs. The concentration of LHE used in this study was in the range of 0.003 to 1.0 wt%. Aqueous colloidal solutions of AgNPs reduced and stabilized by LHE show long-term stability due to the steric stabilization effect. This can be confirmed by zeta potential measurements which afforded values approximately of 0 mV, indicating the steric stability of AgNPs colloidal solutions synthesized by LHE. Furthermore, the obtained AgNPs colloidal solutions show superior antibacterial effect to gram-positive bacteria (Staphylococcus aureus) comparing to Chloramphenicol (positive control). AgNPs with LHE 0.003 wt% affords the highest antibacterial effect to S. aureus showing an inhibition zone diameter of 19.08 ± 0.67 mm; which is superior to Chloramphenicol.

scholarly journals Green Synthesis of Silver Nanoparticles using Vitex Negundo Extracts and their Application in the Effluent Treatment of Cracker Industries

2021 ◽  
Vol 2070 (1) ◽  
pp. 012034
Author(s):  
G Kanthimathi ◽  
O Senthilkumar ◽  
C Sankar ◽  
B.S. Prathibha ◽  
S.M. Senthil Kumar
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Room Temperature ◽  
Industrial Effluents ◽  
Effluent Treatment ◽  
Poly Vinyl Alcohol ◽  
Vitex Negundo ◽  
X Ray Diffraction ◽  
Inexpensive Method ◽  
X Ray

Abstract Silver nanoparticles were prepared by green synthesis, which is an eco-friendly and inexpensive method. The synthesis was carried out using Vitex Negundo leaf extract at room temperature. The nanoparticles were encapsulated with Poly Vinyl Alcohol (PVA) matrix to avoid agglomeration. The formation of silver nanoparticles was confirmed by X-ray Diffraction (XRD). The morphology of the nanoparticles was investigated by scanning electron microscope (SEM). Energy Dispersive X-ray Spectrum (EDX) confirmed the presence of elemental Ag. Adsorption experiments confirmed the removal of toxic cadmium and chromium present in the industrial effluents as analysed by atomic absorption spectroscopy. The silver nanoparticles showed maximum adsorption efficiency for chromium compared to cadmium.

scholarly journals PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS – ANTIBACTERIAL EFFECT

2020 ◽  
Author(s):  
Branislav RUTTKAY-NEDECKÝ ◽  
Karel SEHNAL ◽  
Dominik BANAS ◽  
Martina STANKOVA ◽  
Marta KEPINSKA ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Effect
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