scholarly journals Design and Screening of M13 Phage Display cDNA Libraries

Molecules ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 1667-1681 ◽  
Author(s):  
Yuliya Georgieva ◽  
Zoltán Konthur
Keyword(s):  
Phage Display ◽  
Cdna Libraries ◽  
M13 Phage

scholarly journals Selection of Peptides Targeting Helix 31 of Bacterial 16S Ribosomal RNA by Screening M13 Phage-Display Libraries

Molecules ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 1211-1239 ◽  
Author(s):  
Tek N. Lamichhane ◽  
N. Dinuka Abeydeera ◽  
Anne-Cécile E. Duc ◽  
Philip R. Cunningham ◽  
Christine S. Chow
Keyword(s):  
Phage Display ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Phage Display Libraries ◽  
M13 Phage ◽  
Selection Of

scholarly journals Identification of peroxisomal proteins by using M13 phage protein VI phage display: molecular evidence that mammalian peroxisomes contain a 2,4-dienoyl-CoA reductase

1999 ◽  
Vol 340 (2) ◽  
pp. 561-568 ◽  
Author(s):  
Marc FRANSEN ◽  
Paul P. VAN VELDHOVEN ◽  
Suresh SUBRAMANI
Keyword(s):  
Mammalian Cells ◽  
Unsaturated Fatty Acids ◽  
Urate Oxidase ◽  
Cdna Libraries ◽  
Molecular Evidence ◽  
Cdna Clones ◽  
Novel Proteins ◽  
M13 Phage ◽  
Coa Reductase ◽  
Protein Vi

To elucidate unknown mammalian peroxisomal enzymes and functions, we subjected M13 phage expressing fusions between the gene encoding protein VI and a rat liver cDNA library to an immunoaffinity selection process in vitro (biopanning) with the use of antibodies raised against peroxisomal subfractions. In an initial series of biopanning experiments, four different cDNA clones were obtained. These cDNA species encoded two previously identified peroxisomal enzymes, catalase and urate oxidase, and two novel proteins that contained a C-terminal peroxisomal targeting signal (PTS1). A primary structure analysis of these novel proteins revealed that one, ending in the tripeptide AKL, is homologous to the yeast peroxisomal 2,4-dienoyl-CoA reductase (EC 1.3.1.34; DCR), an enzyme required for the degradation of unsaturated fatty acids, and that the other, ending in the tripeptide SRL, is a putative member of the short-chain dehydrogenase/reductase (SDR) family, with three isoforms. Green fluorescent protein (GFP) fusions encoding GFP-DCR-AKL, GFP-DCR, GFP-SDR-SRL and GFP-SDR were expressed in mammalian cells. The analysis of the subcellular location of the recombinant fusion proteins confirmed the peroxisomal localization of GFP-DCR-AKL and GFP-SDR-SRL, as well as the functionality of the PTS1. That the AKL protein is indeed an NADPH-dependent DCR was demonstrated by showing DCR activity of the bacterially expressed protein. These results demonstrate at the molecular level that mammalian peroxisomes do indeed contain a DCR. In addition, the results presented here indicate that the protein VI display system is suitable for the isolation of rare cDNA clones from cDNA libraries and that this technology facilitates the identification of novel peroxisomal proteins.

scholarly journals Oligopeptide M13 Phage Display in Pathogen Research

Viruses ◽  
2013 ◽  
Vol 5 (10) ◽  
pp. 2531-2545 ◽  
Author(s):  
Jonas Kügler ◽  
Jonas Zantow ◽  
Torsten Meyer ◽  
Michael Hust
Keyword(s):  
Phage Display ◽  
M13 Phage

scholarly journals Analysis of peptide‐binding motifs for two disease associated HLA‐DR13 alleles using an M13 phage display library

Immunology ◽  
1996 ◽  
Vol 88 (4) ◽  
pp. 482-486 ◽  
Author(s):  
M. P. DAVENPORT ◽  
C. L. QUINN ◽  
P. VALSASNINI ◽  
F. SINIGAGLIA ◽  
A. V. S. HILL ◽  
...  
Keyword(s):  
Phage Display ◽  
Peptide Binding ◽  
Phage Display Library ◽  
Binding Motifs ◽  
M13 Phage

A target-unrelated peptide in an M13 phage display library traced to an advantageous mutation in the gene II ribosome-binding site

2008 ◽  
Vol 373 (1) ◽  
pp. 88-98 ◽  
Author(s):  
Leighanne A. Brammer ◽  
Benjamin Bolduc ◽  
Jessica L. Kass ◽  
Kristin M. Felice ◽  
Christopher J. Noren ◽  
...  
Keyword(s):  
Phage Display ◽  
Binding Site ◽  
Phage Display Library ◽  
Ribosome Binding Site ◽  
Ribosome Binding ◽  
M13 Phage

Phage Display Selection of P1 Mutants of BPTI Directed against Five Different Serine Proteinases

1999 ◽  
Vol 380 (1) ◽  
Author(s):  
L. Kiczak ◽  
K. Koscielska ◽  
J. Otlewski ◽  
M. Czerwinski ◽  
M. Dadlez
Keyword(s):  
Phage Display ◽  
Trypsin Inhibitor ◽  
Association Constant ◽  
Serine Proteinases ◽  
Wild Type ◽  
Association Energy ◽  
Basic Pancreatic Trypsin Inhibitor ◽  
Pancreatic Trypsin Inhibitor ◽  
M13 Phage ◽  
Selection Of

AbstractThe P1 position of protein inhibitors and oligopeptide substrates determines, to a large extent, association energy with many serine proteinases. To test the agreement of phage display selection with the existing thermodynamic data, a small library of all 20 P1 mutants of basic pancreatic trypsin inhibitor (BPTI) was created, fused to protein III, and displayed on the surface of M13 phage. The wild type of displayed inhibitor monovalently and strongly inhibited trypsin with an association constant of

Identification of a human immunodominant B-cell epitope within the GRA1 antigen of Toxoplasma gondii by phage display of cDNA libraries

2001 ◽  
Vol 31 (14) ◽  
pp. 1659-1668 ◽  
Author(s):  
Elisa Beghetto ◽  
Andrea Pucci ◽  
Olga Minenkova ◽  
Andrea Spadoni ◽  
Luca Bruno ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Phage Display ◽  
B Cell ◽  
Cell Epitope ◽  
Cdna Libraries ◽  
B Cell Epitope
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