scholarly journals Rapid Identification of Escherichia coli Colistin-Resistant Strains by MALDI-TOF Mass Spectrometry

2021 ◽  
Vol 9 (11) ◽  
pp. 2210
Author(s):  
Adriana Calderaro ◽  
Mirko Buttrini ◽  
Benedetta Farina ◽  
Sara Montecchini ◽  
Monica Martinelli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Rapid Identification ◽  
Global Public Health ◽  
Gold Standard Method ◽  
E Coli ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Lower Ability ◽  
Resistant Strains

Colistin resistance is one of the major threats for global public health, requiring reliable and rapid susceptibility testing methods. The aim of this study was the evaluation of a MALDI-TOF mass spectrometry (MS) peak-based assay to distinguish colistin resistant (colR) from susceptible (colS) Escherichia coli strains. To this end, a classifying algorithm model (CAM) was developed, testing three different algorithms: Genetic Algorithm (GA), Supervised Neural Network (SNN) and Quick Classifier (QC). Among them, the SNN- and GA-based CAMs showed the best performances: recognition capability (RC) of 100% each one, and cross validation (CV) of 97.62% and 100%, respectively. Even if both algorithms shared similar RC and CV values, the SNN-based CAM was the best performing one, correctly identifying 67/71 (94.4%) of the E. coli strains collected: in point of fact, it correctly identified the greatest number of colS strains (42/43; 97.7%), despite its lower ability in identifying the colR strains (15/18; 83.3%). In conclusion, although broth microdilution remains the gold standard method for testing colistin susceptibility, the CAM represents a useful tool to rapidly screen colR and colS strains in clinical practice.

scholarly journals Fast and robust detection of colistin resistance in Escherichia coli using the MALDI Biotyper Sirius mass spectrometry system

2019 ◽  
Author(s):  
R. Christopher D. Furniss ◽  
Laurent Dortet ◽  
William Bolland ◽  
Oliver Drews ◽  
Katrin Sparbier ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Cost Effective ◽  
Colistin Resistance ◽  
Robust Detection ◽  
E Coli ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Maldi Biotyper ◽  
Mass Spectrometry System

ABSTRACTPolymyxin antibiotics are a last-line treatment for multidrug-resistant Gram-negative bacteria. However, the emergence of colistin resistance, including the spread of mobile mcr genes, necessitates the development of improved diagnostics for the detection of colistin-resistant organisms in hospital settings. The recently developed MALDIxin test enables detection of colistin resistance by MALDI-TOF mass spectrometry in less than 15 minutes but is not optimized for the mass spectrometers commonly found in clinical microbiology laboratories. In this study, we adapted the MALDIxin test for the MALDI Biotyper Sirius MALDI-TOF mass spectrometry system (Bruker Daltonics). We optimized the sample preparation protocol using a set of 6 MCR-expressing Escherichia coli clones and validated the assay with a collection of 40 E. coli clinical isolates, including 19 MCR producers, 12 chromosomally-resistant isolates and 9 polymyxin-susceptible isolates. We calculated Polymyxin resistance ratio (PRR) values from the acquired spectra; a PRR value of zero, indicating polymyxin susceptibility, was obtained for all colistin-susceptible E. coli isolates, whereas positive PRR values, indicating resistance to polymyxins, were obtained for all resistant strains independent of the genetic basis of resistance. Thus, we report a preliminary feasibility study showing that an optimized version of the MALDIxin test, adapted for the routine MALDI Biotyper Sirius, provides an unbiased, fast, reliable, cost-effective and high-throughput way of detecting colistin resistance in clinical E. coli isolates.

Catheter-related Mycobacterium fortuitum Bloodstream Infection: Rapid Identification Using MALDI-TOF Mass Spectrometry

2014 ◽  
Vol 226 (02) ◽  
pp. 68-71 ◽  
Author(s):  
M. Artacho-Reinoso ◽  
P. Olbrich ◽  
P. Solano-Paéz ◽  
P. Ybot-Gonzalez ◽  
J. Lepe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bloodstream Infection ◽  
Rapid Identification ◽  
Mycobacterium Fortuitum ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals A rapid diagnostic workflow for cefotaxime-resistant Escherichia coli and Klebsiella pneumoniae detection from blood cultures by MALDI-TOF mass spectrometry

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0185935 ◽  
Author(s):  
Elena De Carolis ◽  
Silvia Paoletti ◽  
Domenico Nagel ◽  
Antonietta Vella ◽  
Enrica Mello ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Blood Cultures ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Erysipelothrix rhusiopathiae bacteremia without endocarditis: rapid identification from positive blood culture by MALDI-TOF mass spectrometry. A case report and literature review

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Luigi Principe ◽  
Silvia Bracco ◽  
Carola Mauri ◽  
Silvia Tonolo ◽  
Beatrice Pini ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Susceptibility Testing ◽  
Bloodstream Infections ◽  
Rapid Identification ◽  
Erysipelothrix Rhusiopathiae ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
High Incidence ◽  
Appropriate Therapy ◽  
Cutaneous Form

<em>Erysipelothrix</em> <em>rhusiopathiae</em> is a Gram-positive bacillus that is infrequently responsible for infections in humans. Three forms have been classified: a localized cutaneous form (erysipeloid) caused by traumatic penetration of <em>E.</em> <em>rhusiopathiae</em>, a generalized cutaneous form and a septicemic form. The latter type of disease has been previously associated with a high incidence of endocarditis. Here we report a case of <em>E. rhusiopathiae</em> bacteremia in a 74- year-old man, probably started from an erysipeloid form, in which endocarditis did not develop. This case presents some particular and uncommon features: i) no correlation with animal source; ii) correlation between bacteremia and erysipeloid lesion; iii) absence of endocarditis. MALDI-TOF mass spectrometry allowed to obtain a rapid identification (within 4 hours from bottle positivity) of <em>E. rhusiopathiae</em>. Together with direct antimicrobial susceptibility testing, this approach could improve the rate of appropriate therapy for bloodstream infections due to this fastidious pathogen.

scholarly journals Use of MALDI-TOF mass spectrometry in rapid identification of Beauveria bassiana and Beauveria pseudobassiana

2018 ◽  
Vol 19 (2) ◽  
pp. 394-407 ◽  
Author(s):  
Nikola HRICÁKOVÁ ◽  
Juraj MEDO ◽  
Lukáš HLEBA ◽  
Marek BARTA ◽  
Jana MAKOVÁ
Keyword(s):  
Mass Spectrometry ◽  
Beauveria Bassiana ◽  
Rapid Identification ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

MALDI-TOF mass spectrometry for rapid identification of clinical fungal isolates based on ribosomal protein biomarkers

2015 ◽  
Vol 109 ◽  
pp. 93-105 ◽  
Author(s):  
Ashutosh Panda ◽  
Anup K. Ghosh ◽  
Bijay R. Mirdha ◽  
Immaculata Xess ◽  
Saikat Paul ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ribosomal Protein ◽  
Rapid Identification ◽  
Protein Biomarkers ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Fungal Isolates

Rapid Identification of Staphylococci from Prosthetic Joint Infections Using MALDI-TOF Mass-Spectrometry

2010 ◽  
Vol 33 (9) ◽  
pp. 568-574 ◽  
Author(s):  
Llinos G. Harris ◽  
Khalid El-Bouri ◽  
Stuart Johnston ◽  
Eugene Rees ◽  
Lars Frommelt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Rapid Identification ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections
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