scholarly journals Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay

2021 ◽  
Vol 9 (10) ◽  
pp. 2165
Author(s):  
María Laura Félix ◽  
Sebastián Muñoz-Leal ◽  
Luis Andrés Carvalho ◽  
Diego Queirolo ◽  
Susana Remesar ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Pathogenic Role ◽  
Free Living ◽  
Epidemiological Factors ◽  
The Third ◽  
Mazama Gouazoubira ◽  
Potential Pathogenicity ◽  
Human Ehrlichiosis ◽  
Brocket Deer

Human ehrlichiosis are scantily documented in Uruguay. The aim of this study was to investigate the presence of Ehrlichia spp. in Haemaphysalis juxtakochi and in a gray brocket deer (Mazama gouazoubira) from Uruguay. The presence of Ehrlichia DNA was investigated in free-living H. juxtakochi in five localities of southeast and northeast Uruguay, as well as blood, spleen, and ticks retrieved from a M. gouazoubira. Ehrlichia spp. DNA was detected in six out of 99 tick pools from vegetation, in the spleen of M. gouazoubira, and in one out of five pools of ticks feeding on this cervid. Bayesian inference analyses for three loci (16S rRNA, dsb, and groEL) revealed the presence of a new rickettsial organism, named herein as “Candidatus Ehrlichia pampeana”. This new detected Ehrlichia is phylogenetically related to those found in ticks from Asia, as well as Ehrlichia ewingii from USA and Cameroon. Although the potential pathogenicity of “Ca. E. pampeana” for humans is currently unknown, some eco-epidemiological factors may be relevant to its possible pathogenic role, namely: (i) the phylogenetic closeness with the zoonotic agent E. ewingii, (ii) the evidence of H. juxtakochi parasitizing humans, and (iii) the importance of cervids as reservoirs for zoonotic Ehrlichia spp. The molecular detection of “Ca. E. pampeana” represents the third Ehrlichia genotype described in Uruguay.

scholarly journals Post-mortem hemoparasite detection in free-living Brazilian brown brocket deer (Mazama gouazoubira, Fischer 1814)

2014 ◽  
Vol 23 (2) ◽  
pp. 206-215 ◽  
Author(s):  
Júlia Angélica Gonçalves da Silveira ◽  
Élida Mara Leite Rabelo ◽  
Paula Cristina Senra Lima ◽  
Bárbara Neves Chaves ◽  
Múcio Flávio Barbosa Ribeiro
Keyword(s):  
Nested Pcr ◽  
Pcr Analysis ◽  
Post Mortem ◽  
Infectious Agents ◽  
Free Living ◽  
Molecular Assays ◽  
Minas Gerais State ◽  
Mazama Gouazoubira ◽  
Nested Pcr Assays ◽  
Brocket Deer

Tick-borne infections can result in serious health problems for wild ruminants, and some of these infectious agents can be considered zoonosis. The aim of the present study was the post-mortem detection of hemoparasites in free-living Mazama gouazoubira from Minas Gerais state, Brazil. The deer samples consisted of free-living M. gouazoubira (n = 9) individuals that died after capture. Necropsy examinations of the carcasses were performed to search for macroscopic alterations. Organ samples were collected for subsequent imprint slides, and nested PCR assays were performed to detect hemoparasite species. Imprint slide assays from four deer showed erythrocytes infected with Piroplasmida small trophozoites, and A. marginale corpuscles were observed in erythrocytes from two animals. A. marginale and trophozoite co-infections occurred in two deer. A nested PCR analysis of the organs showed that six of the nine samples were positive for Theileria sp., five were positive for A. phagocytophilum and three were positive for A. marginale, with co-infection occurring in four deer. The results of the present study demonstrate that post-mortemdiagnostics using imprint slides and molecular assays are an effective method for detecting hemoparasites in organs.

Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer ( Mazama gouazoubira , Fischer, 1814)

2020 ◽  
Author(s):  
Duanny Murinelly de Souza Cunha ◽  
Mírley Barbosa de Souza ◽  
Bruna Farias Brito ◽  
Herlon Victor Rodrigues Silva ◽  
Leandro Rodrigues Ribeiro ◽  
...  
Keyword(s):  
Seminal Parameters ◽  
Sperm Morphometry ◽  
Mazama Gouazoubira ◽  
Brocket Deer

scholarly journals Localization and Functional Characterization of the Alternative Oxidase in Naegleria

2020 ◽  
Author(s):  
Diego Cantoni ◽  
Ashley Osborne ◽  
Najwa Taib ◽  
Gary Thompson ◽  
Eleanna Kazana ◽  
...  
Keyword(s):  
Cell Biology ◽  
Structure Prediction ◽  
Alternative Oxidase ◽  
Functional Characterization ◽  
Krebs Cycle ◽  
Last Common Ancestor ◽  
Free Living ◽  
And Function ◽  
Potential Pathogenicity

ABSTRACTThe Alternative oxidase (AOX) is a protein involved in maintaining the Krebs cycle in instances where the respiratory chain has been inhibited, while allowing for the maintenance of cell growth and necessary metabolic processes for survival. Among eukaryotes, alternative oxidases have disperse distribution and are found in plants, fungi and a few protists, including Naegleria ssp. Naegleria species are free-living unicellular amoeboflagellates, and include the pathogenic species of N. fowleri, the so-called brain eating amoeba. Using a multidisciplinary approach, we aimed to understand the evolution, localization and function of AOX and the role that plays in Naegleria’s biology. Our analyses suggest that the protein was present in last common ancestor of the genus and structure prediction showed that all functional residues are also present in Naegleria species. Using a combination of cellular and biochemical techniques, we also functionally characterize N. gruberi’s AOX in its mitochondria and we demonstrate that its inactivation affects its proliferation. Consequently, we discuss the benefits of the presence of this protein in Naegleria species, along with its potential pathogenicity role in N. fowleri. We predict that our findings will spearhead new explorations to understand the cell biology, metabolism and evolution of Naegleria and other free-living relatives.

Molecular characterization of putative Hepatozoon sp. from the sedge warbler (Acrocephalus schoenobaenus)

Parasitology ◽  
2013 ◽  
Vol 140 (6) ◽  
pp. 695-698 ◽  
Author(s):  
ALEKSANDRA BIEDRZYCKA ◽  
AGNIESZKA KLOCH ◽  
MAGDALENA MIGALSKA ◽  
WOJCIECH BIELAŃSKI
Keyword(s):  
Molecular Characterization ◽  
18S Rdna ◽  
Molecular Detection ◽  
Phylogenetic Analyses ◽  
Systematic Position ◽  
The Third ◽  
Sedge Warbler ◽  
Blood Smears

SUMMARYWe characterized partial sequences of 18S rDNA from sedge warblers infected with a parasite described previously as Hepatozoon kabeeni. Prevalence was 47% in sampled birds. We detected 3 parasite haplotypes in 62 sequenced samples from infected animals. In phylogenetic analyses, 2 of the putative Hepatozoon haplotypes closely resembled Lankesterella minima and L. valsainensis. The third haplotype grouped in a wider clade composed of Caryospora and Eimeria. None of the haplotypes showed resemblance to sequences of Hepatozoon from reptiles and mammals. Molecular detection results were consistent with those from microscopy of stained blood smears, confirming that the primers indeed amplified the parasite sequences. Here we provide evidence that the avian Hepatozoon-like parasites are most likely Lankesterella, supporting the suggestion that the systematic position of avian Hepatozoon-like species needs to be revised.

Gaunilo Parodies Anselm

2014 ◽  
Vol 17 (1) ◽  
pp. 45-71
Author(s):  
Geo Siegwart
Keyword(s):  
Detailed Comparison ◽  
The Third ◽  
Common Structure ◽  
Logical Reconstruction ◽  
Points Of View ◽  
And Function

The main objective is an interpretation of the island parody, in particular a logical reconstruction of the parodying argument that stays close to the text. The parodied reasoning is identified as the proof in the second chapter of the Proslogion, more specifically, this proof as it is represented by Gaunilo in the first chapter of his Liber pro insipiente. The second task is a detailed comparison between parodied and parodying argument as well as an account of their common structure. The third objective is a tentative characterization of the nature and function of parodies of arguments. It seems that parodying does not add new pertinent points of view to the usual criticism of an argument.

Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines

2021 ◽  
Vol 199 ◽  
pp. 106383
Author(s):  
Innina R. Mananguit ◽  
Nest Dale F. Bartolome ◽  
Gabriel Alexis S.P. Tubalinal ◽  
Claro N. Mingala
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
The Philippines
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