scholarly journals Wastewater from the Edible Oil Industry as a Potential Source of Lipase- and Surfactant-Producing Actinobacteria

2021 ◽  
Vol 9 (9) ◽  
pp. 1987
Author(s):  
Pamela Welz ◽  
Gustav Swanepoel ◽  
Shandré Weels ◽  
Marilize Le Roes-Hill
Keyword(s):  
Genome Sequencing ◽  
Crude Protein ◽  
Oxygen Demand ◽  
Gene Clusters ◽  
Lipase Production ◽  
Edible Oil ◽  
Novel Approach ◽  
Cutibacterium Acnes ◽  
Wastewater Samples ◽  
High Chemical Oxygen Demand

Wastewaters generated from various stages of edible oil production in a canola processing facility were collected with the aim of determining the presence of lipase-producing actinobacteria of potential industrial significance. The high chemical oxygen demand (COD) readings (up to 86,700 mg L−1 in some samples) indicated that the wastewater exhibited the nutritional potential to support bacterial growth. A novel approach was developed for the isolation of metagenomic DNA from the oil-rich wastewater samples. Microbiota analysis of the buffer tank and refinery condensate tank wastewater samples showed a dominance of Cutibacterium acnes subsp. defendens, followed by a limited number of other actinobacterial genera, indicating the presence of a highly specialized actinobacterial population. Cultured isolates with typical actinobacterial morphology were analyzed for their ability to produce lipases and biosurfactants. Two strains, designated as BT3 and BT4, exhibited the highest lipase production levels when grown in the presence of tributyrin and olive oil (1.39 U mg−1 crude protein and 0.8 U mg−1 crude protein, respectively) and were subsequently definitively identified by genome sequencing to be related to Streptomyces albidoflavus. Cultivation of the strains in media containing different types of oils did not markedly increase the level of enzyme production, with the exception of strain BT4 (1.0 U mg−1 crude protein in the presence of peanut oil). Genome sequencing of the two strains, BT3 and BT4, revealed the presence of a range of lipase and esterase genes that may be involved in the production of the enzymes detected in this study. The presence of gene clusters involved in the production of biosurfactants were also detected, notably moreso in strain BT3 than BT4.

scholarly journals Membrane bioreactor for mall wastewater treatment

2020 ◽  
Vol 15 (2) ◽  
pp. 1
Author(s):  
Everton Luis Butzen ◽  
Gabriel Capellari Santos ◽  
Sandrini Slongo Fortuna ◽  
Vandré Barbosa Brião
Keyword(s):  
Phosphorus Removal ◽  
Membrane Bioreactor ◽  
Constant Pressure ◽  
Oxygen Demand ◽  
Previous Treatment ◽  
Medium Size ◽  
Permeate Flux ◽  
Urban Wastewater ◽  
Wastewater Samples ◽  
High Chemical Oxygen Demand

 Malls concentrate a large number of people in a relatively small area, and thus generate concentrated urban wastewater. This study evaluated the performance of a membrane bioreactor (MBR) as an alternative for the treatment of mall wastewater. Wastewater samples without any previous treatment were collected from a medium-size mall and showed a high chemical oxygen demand (COD) near 2,000 mg L-1. The MBR operated with a constant pressure of 40 kPa during 60 days with a sludge age of 30 days.  Concentration of biomass was 3,738±930 mg L-1 and average permeate flux was 7.0 L h-1 m-2. The MBR was able to remove 91.2% and 97.2% of color and turbidity, respectively. Furthermore, COD removal was approximately 90% and biochemical oxygen demand (BOD) 88%. In addition, the MBR produced a phosphorus removal near 50%, and for nitrogen, 80%. The MBR system proved to be an efficient process for the removal of the pollutants, remaining stable even with the oscillation of the characteristics of the raw sewage, presenting great potential for application in the treatment of sewage from malls and effluents with high organic loads.

Sewage Treatment by Waste Stabilization Pond Systems

2018 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
J. Kenneth, R. S. Suglo
Keyword(s):  
Management Practices ◽  
Sewage Treatment ◽  
Oxygen Demand ◽  
Wastewater Management ◽  
Stabilization Pond ◽  
Waste Stabilization Pond ◽  
Quality Guidelines ◽  
Waste Stabilization ◽  
High Reduction ◽  
Wastewater Samples

Sewage generated in Ghana is commonly discharged into the environment without any form of treatment to reduce the degree ofcontamination and mitigate potential public health and environmental issues. Although some attempts have been made in someparts of Ghana to utilize the waste stabilization pond (WSP) system to treat domestic sewage, the ponds often fail to achievetheir purpose due to lack of basic maintenance and supervision. To assess the utility of the WSP system for treating sewage,wastewater samples were collected from the raw sewage, anaerobic, facultative and maturation ponds of WSPs at Obuasi inGhana, and analyzed for physicochemical and microbiological contaminants. The results show that the final pond effluent meetsrecommended microbiological and chemical quality guidelines. The waste stabilization pond system demonstrates high removalefficiencies of wastewater contaminants. The biochemical oxygen demand, total suspended solids, nitrate and faecal coliformsreduction efficiencies of 97.3%, 97.6%, 83.3% and 99.94% respectively are highly significant, and compare well with reportedremoval efficiencies in the literature. Additionally, the ponds have high reduction efficiencies for heavy metals and pathogenicmicroorganisms. The wastewater treatment system complies with standard wastewater management practices, and provides auseful method for treating and disposing wastewater in Ghana.

Some Physicochemical Parameters of Wastewater from an Oil Industry at Point of Discharge in Port Harcourt, Rivers State, Nigeria

2020 ◽  
Vol 6 (2) ◽  
pp. 23-28
Author(s):  
O. A. F Wokoma ◽  
◽  
O. S Edori ◽  
Keyword(s):  
Physicochemical Parameters ◽  
Oxygen Demand ◽  
Aquatic Organisms ◽  
Oil Industry ◽  
World Health ◽  
Port Harcourt ◽  
Water Association ◽  
Wastewater Samples ◽  
Health Organization ◽  
Rivers State

Wastewater samples were collected from an oil industry at the point of discharge for a period of two years, from January 2018 – December 2019. The wastewater samples were analyzed for different physicochemical parameters such as temperature, turbidity, total dissolved solids (TDS), total suspended solids (TSS), conductivity, pH, alkalinity, salinity, total hydrocarbon content (THC), biochemical oxygen demand (BOD) and chemical oxygen demand (COD) to examine their conformity to fulfill requirements as recommended by World Health Organization (WHO), Federal Ministry of Environment (FME) and Drinking Water Association (DWA). The results indicated that all the parameters in the discharged wastewater were within acceptable limits of the regulatory bodies. The field data showed that the investigated firm conformed to the law by carrying out proper procedures before discharging the effluents into the public drain and river. Therefore, the release of wastewater from the industry doesn't constitute a danger to the environment as well as aquatic organisms. Keywords: Physicochemical parameter, wastewater discharge, oil industry, environment, contaminants

scholarly journals Global biogeographic sampling of bacterial secondary metabolism

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Zachary Charlop-Powers ◽  
Jeremy G Owen ◽  
Boojala Vijay B Reddy ◽  
Melinda A Ternei ◽  
Denise O Guimarães ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Genome Sequencing ◽  
Geographic Distance ◽  
Gene Clusters ◽  
Natural World ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Road Map ◽  
Two Factors ◽  
Natural Products Discovery

Recent bacterial (meta)genome sequencing efforts suggest the existence of an enormous untapped reservoir of natural-product-encoding biosynthetic gene clusters in the environment. Here we use the pyro-sequencing of PCR amplicons derived from both nonribosomal peptide adenylation domains and polyketide ketosynthase domains to compare biosynthetic diversity in soil microbiomes from around the globe. We see large differences in domain populations from all except the most proximal and biome-similar samples, suggesting that most microbiomes will encode largely distinct collections of bacterial secondary metabolites. Our data indicate a correlation between two factors, geographic distance and biome-type, and the biosynthetic diversity found in soil environments. By assigning reads to known gene clusters we identify hotspots of biomedically relevant biosynthetic diversity. These observations not only provide new insights into the natural world, they also provide a road map for guiding future natural products discovery efforts.

scholarly journals Efficiency and Technological Reliability of Contaminant Removal in Household WWTPs with Activated Sludge

2021 ◽  
Vol 11 (4) ◽  
pp. 1889 ◽  
Author(s):  
Agnieszka Micek ◽  
Krzysztof Jóźwiakowski ◽  
Michał Marzec ◽  
Agnieszka Listosz ◽  
Tadeusz Grabowski
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Oxygen Demand ◽  
Domestic Wastewater ◽  
Pollutant Removal ◽  
Treated Wastewater ◽  
Contaminant Removal ◽  
Nitrogen And Phosphorus ◽  
Wastewater Samples ◽  
Polish Law

The results of research on the efficiency and technological reliability of domestic wastewater purification in two household wastewater treatment plants (WWTPs) with activated sludge are presented in this paper. The studied facilities were located in the territory of the Roztocze National Park (Poland). The mean wastewater flow rate in the WWTPs was 1.0 and 1.6 m3/day. In 2017–2019, 20 series of analyses were done, and 40 wastewater samples were taken. On the basis of the received results, the efficiency of basic pollutant removal was determined. The technological reliability of the tested facilities was specified using the Weibull method. The average removal efficiencies for the biochemical oxygen demand in 5 days (BOD5) and chemical oxygen demand (COD) were 66–83% and 62–65%, respectively. Much lower effects were obtained for total suspended solids (TSS) and amounted to 17–48%, while the efficiency of total phosphorus (TP) and total nitrogen (TN) removal did not exceed 34%. The analyzed systems were characterized by the reliability of TSS, BOD5, and COD removal at the level of 76–96%. However, the reliability of TN and TP elimination was less than 5%. Thus, in the case of biogenic compounds, the analyzed systems did not guarantee that the quality of treated wastewater would meet the requirements of the Polish law during any period of operation. This disqualifies the discussed technological solution in terms of its wide application in protected areas and near lakes, where the requirements for nitrogen and phosphorus removal are high.

scholarly journals Whole-genome sequencing of the endemic Antarctic fungus Antarctomyces pellizariae reveals an ice-binding protein, a scarce set of secondary metabolites gene clusters and provides insights on Thelebolales phylogeny

Genomics ◽  
2020 ◽  
Vol 112 (5) ◽  
pp. 2915-2921 ◽  
Author(s):  
Thiago Mafra Batista ◽  
Heron Oliveira Hilario ◽  
Gabriel Antônio Mendes de Brito ◽  
Rennan Garcias Moreira ◽  
Carolina Furtado ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Binding Protein ◽  
Protein A ◽  
Gene Clusters ◽  
Whole Genome ◽  
Ice Binding ◽  
Ice Binding Protein

scholarly journals Treatment performances of French constructed wetlands: results from a database collected over the last 30 years

2015 ◽  
Vol 71 (9) ◽  
pp. 1333-1339 ◽  
Author(s):  
A. Morvannou ◽  
N. Forquet ◽  
S. Michel ◽  
S. Troesch ◽  
P. Molle
Keyword(s):  
Constructed Wetlands ◽  
Oxygen Demand ◽  
Pollutant Removal ◽  
Vertical Flow ◽  
Removal Rates ◽  
Waste Stabilization Ponds ◽  
Small Communities ◽  
Technical Department ◽  
In Series ◽  
High Chemical Oxygen Demand

Approximately 3,500 constructed wetlands (CWs) provide raw wastewater treatment in France for small communities (<5,000 people equivalent). Built during the past 30 years, most consist of two vertical flow constructed wetlands (VFCWs) in series (stages). Many configurations exist, with systems associated with horizontal flow filters or waste stabilization ponds, vertical flow with recirculation, partially saturated systems, etc. A database analyzed 10 years earlier on the classical French system summarized the global performances data. This paper provides a similar analysis of performance data from 415 full-scale two-stage VFCWs from an improved database expanded by monitoring data available from Irstea and the French technical department. Trends presented in the first study are confirmed, exhibiting high chemical oxygen demand (COD), total suspended solids (TSS) and total Kjeldahl nitrogen (TKN) removal rates (87%, 93% and 84%, respectively). Typical concentrations at the second-stage outlet are 74 mgCOD L−1, 17 mgTSS L−1 and 11 mgTKN L−1. Pollutant removal performances are summarized in relation to the loads applied at the first treatment stage. While COD and TSS removal rates remain stable over the range of applied loads, the spreading of TKN removal rates increases as applied loads increase.

Characterization of slowly-biodegradable organic compounds and hydrolysis kinetics in tropical wastewater for biological nitrogen removal

2020 ◽  
Vol 81 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Seow Wah How ◽  
Jia Huey Sin ◽  
Sharon Ying Ying Wong ◽  
Pek Boon Lim ◽  
Alijah Mohd Aris ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Uptake Rate ◽  
Wastewater Treatment Plants ◽  
Oxygen Demand ◽  
Hydrolysis Rate ◽  
Biological Nitrogen Removal ◽  
Tropical Region ◽  
Kinetic Coefficients ◽  
Wastewater Samples ◽  
Biological Nitrogen

Abstract Many developing countries, mostly situated in the tropical region, have incorporated a biological nitrogen removal process into their wastewater treatment plants (WWTPs). Existing wastewater characteristic data suggested that the soluble chemical oxygen demand (COD) in tropical wastewater is not sufficient for denitrification. Warm wastewater temperature (30 °C) in the tropical region may accelerate the hydrolysis of particulate settleable solids (PSS) to provide slowly-biodegradable COD (sbCOD) for denitrification. This study aimed to characterize the different fractions of COD in several sources of low COD-to-nitrogen (COD/N) tropical wastewater. We characterized the wastewater samples from six WWTPs in Malaysia for 22 months. We determined the fractions of COD in the wastewater by nitrate uptake rate experiments. The PSS hydrolysis kinetic coefficients were determined at tropical temperature using an oxygen uptake rate experiment. The wastewater samples were low in readily-biodegradable COD (rbCOD), which made up 3–40% of total COD (TCOD). Most of the biodegradable organics were in the form of sbCOD (15–60% of TCOD), which was sufficient for complete denitrification. The PSS hydrolysis rate was two times higher than that at 20 °C. The high PSS hydrolysis rate may provide sufficient sbCOD to achieve effective biological nitrogen removal at WWTPs in the tropical region.

scholarly journals Physicochemistry and Heavy Metal Characteristics of Waste Products from Abattoir Activities in Port Harcourt, Nigeria

2019 ◽  
pp. 1-12
Author(s):  
Azuonwu, Testimonies Chikanka ◽  
David N. Ogbonna ◽  
John Onwuteaka
Keyword(s):  
Water Samples ◽  
Oxygen Demand ◽  
Aquatic Organisms ◽  
Aquatic Life ◽  
Waste Products ◽  
Faecal Matter ◽  
Port Harcourt ◽  
Service Water ◽  
Significant Difference ◽  
Wastewater Samples

The upsurge of abattoir operations as a result of the rise in demand for meat protein has led to a corresponding increase in waste generation. These wastes are often channelled into nearby streams with little or no treatment which exposes aquatic organisms to the resultant consequences of this waste deposition. This study was thus aimed at determining the physicochemical quality of some abattoir samples in Port Harcourt city. Soil, faecal matter, wastewater, waste blood and service water samples from the Iwofe, Rumuodomaya and Trans-Amadi abattoirs were collected within a  period of one year and the pH, chemical oxygen demand (COD), biochemical oxygen demand (BOD5), sulphate, chloride, temperature, nitrate, ammonia and heavy metals quantities including chromium, lead, zinc, cobalt, copper and cadmium were determined using standard techniques. The study recorded high BOD5 and COD values from the blood, faecal matter, soil and wastewater samples while service water samples had values within the Nigerian Standard for Drinking Water Quality limits. Seasonal variations in the physicochemical parameters of the samples collected both in the wet and dry seasons were observed. The pH values ranged from 6.2-8.5, BOD5 of blood, faecal matter soil and wastewater ranged from 2124-4349 mg/l while the COD ranged from 2715-8820 mg/l. In Rumuodomaya, the BOD5 and COD ranged from 2276-2727 mg/l and 2583-3245 mg/l respectively while samples from Trans-Amadi abattoir had values for BOD5 and COD ranging from 2253-4330 mg/l and 2931-4597 mg/l, respectively. Nitrate, chromium, cadmium, zinc, cobalt, lead and copper contents in the different locations varied statistically at α-0.05 while no significant difference was observed for pH, ammonia, temperature, COD, BOD5, sulphate and chloride contents at α=0.05. The BOD5 and COD of waste blood, wastewater, soil and faecal matter recorded values that were above permissible limits for service water and therefore raises concern for the aquatic life being threatened by these effluents as the amount of dissolved oxygen available for them will be reduced as a pollution of the immediate environment, if these generated wastes are not treated before disposal.    

scholarly journals Comparative Studies of Oleaginous Fungal Strains (Mucor circinelloides and Trichoderma reesei) for Effective Wastewater Treatment and Bio-Oil Production

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Anshuman Bhanja ◽  
Gauri Minde ◽  
Sandip Magdum ◽  
V. Kalyanraman
Keyword(s):  
Wastewater Treatment ◽  
Trichoderma Reesei ◽  
Oxygen Demand ◽  
Oil Production ◽  
Mucor Circinelloides ◽  
Waste To Energy ◽  
Nitrogenous Compounds ◽  
Fungal Strains ◽  
Bio Oil ◽  
Wastewater Samples

Biological wastewater treatment typically requires the use of bacteria for degradation of carbonaceous and nitrogenous compounds present in wastewater. The high lipid containing biomass can be used to extract oil and the contents can be termed as bio-oil (or biodiesel or myco-diesel after transesterification). The separate experiments were conducted on actual wastewater samples with 5% v/v inoculum of Mucor circinelloides MTCC1297 and Trichoderma reesei NCIM992 strains. The observed reductions in chemical oxygen demand (COD) were 88.72% and 86.75% in 96 hrs and the observed substrate based biomass yields were 0.21 mg VSS/mg COD and 0.22 mg VSS/mg COD for M. circinelloides reactor and for T. reesei reactor, respectively. The resulted bio-oil production from wastewater treatment by M. circinelloides and T. reesei reactors was 142.2 mg/L and 74.1 mg/L, whereas biomass containing bio-oil contents (%w/w) were 22.11% and 9.82%, respectively. In this experiment, the fungal wastewater treatment was also compared with conventional bacterial process with respect to specific growth rate, biomass production, and oil content. This study suggests that wastewater can be used as a potential feedstock for bio-oil production with the use of oleaginous fungal strains and which could be a possible route of waste to energy.

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