scholarly journals Evaluation of Locally Isolated Entomopathogenic Fungi against Multiple Life Stages of Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae): Laboratory and Field Study

2021 ◽  
Vol 9 (8) ◽  
pp. 1791
Author(s):  
Muhammad Usman ◽  
Waqas Wakil ◽  
Jaime C. Piñero ◽  
Shaohui Wu ◽  
Michael D. Toews ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Larval Mortality ◽  
Horizontal Transmission ◽  
Adult Emergence ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Life Stages ◽  
Field Cage ◽  
Lethal Effects ◽  
Bactrocera Zonata

Fruit flies including Bactrocera zonata and B. dorsalis (Diptera: Tephritidae) are considered major pests of orchard systems in Pakistan. This study evaluated the laboratory virulence, sub-lethal effects, horizontal transmission, greenhouse, and field-cage efficacy of locally isolated entomopathogenic fungi (EPF) against B. zonata and B. dorsalis. In virulence assays against third instars and adults, all 21 EPF isolates (Beauveria bassiana and Metarhizium anisopliae) tested were pathogenic and caused varying levels of mortality to the fruit flies. Based on the initial screening, four isolates (B. bassiana WG-21 and WG-18 and M. anisopliae WG-07 and WG-02) were selected for further study. The isolate WG-18 was the most virulent against larvae and adults of B. zonata and B. dorsalis followed by WG-21, WG-02, and WG-07. In both species, adults were more susceptible than larvae to all isolates, and pupae were the least susceptible. Isolates WG-18 and WG-21 strongly decreased female fecundity and fertility, the highest adult and larval mortality, and longest developmental time of larvae and pupae. Fungal conidia were disseminated passively from infected to healthy adults and induced significant mortality, particularly from infected males to non-infected females. In greenhouse and field-cage experiments, WG-18 and WG-21 were the most effective isolates in reducing adult emergence when applied to larvae and pupae of both fruit fly species. Our results indicate that B. bassiana isolates WG-18 and WG-21 were the most virulent against multiple life stages of B. zonata and B. dorsalis, and also exerted the strongest sub-lethal effects.

Non-host status of papaya cultivars to the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), in relation to the degree of fruit ripeness

2016 ◽  
Vol 37 (01) ◽  
pp. 19-29 ◽  
Author(s):  
Domingos Cugala ◽  
João Jone Jordane ◽  
Sunday Ekesi
Keyword(s):  
Fruit Ripening ◽  
Market Access ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Laboratory Evaluation ◽  
Ripening Stage ◽  
Field Cage ◽  
Major Barrier ◽  
Ripening Stages

AbstractPhytosanitary measures are a major barrier to trade in papaya. We assessed the infestation of tephritid fruit flies on different stages of maturity of papaya, to determine its non-host stage of maturity, for market access. Papaya fruits were collected from Kilifi and Embu counties, Kenya from March 2013 to December 2014, to assess the level of infestation by fruit flies according to the degree of fruit ripening. In all locations, no fruit fly infestation was recorded on papaya when fruits were at the 0, 25 and 50% yellow fruit ripening stage.Bactrocera dorsalis(Hendel) was, however, observed attacking fruits when papaya fruits were at 75 and 100% all yellow (fully ripe fruit ripening stage) with infestations of 0.19−0.51B. dorsalis/kg fruit and 0.24−1.24B. dorsalis/kg fruit, respectively, in all locations. Field cage exposure ofB. dorsalisto fruits of five papaya cultivars—‘Papino’, ‘Neo Essence’, ‘Sunrise Solo’, ‘Tainung No. 1’ and ‘Tainung No. 2’ in Manica Province, Mozambique—showed thatB. dorsalisdid not infest fruits at 0, 25 and 50% yellow ripening stages at the densities of 50 and 100 flies per cage. However, at 75% yellow ripening stage, up to 13.1 pupae/kg of fruits was recorded at a density of 150 flies per cage in Tainung No. 1, and infestation ranged from 4.5 to 136 pupae/kg fruits at 100% yellow ripening stage across all the cultivars and infestation densities. Laboratory evaluation of volatiles emanating from freshly crushed papaya pulp of four cultivars: ‘Sunrise Solo’, ‘Red Lady’, ‘Papayi’ and ‘Apoyo’ on egg viability ofB. dorsalisshowed that at 0, 25 and 50% yellow, egg hatchability was inhibited, suggesting that semiochemical compounds present in green tissues of papaya prevent egg development, although this effect was variable across the four cultivars and ripening stages. Export papaya is harvested at less than 40% yellow ripening stage. Our results, therefore, suggest that quarantine treatment for fruits at this ripening stage is inconsequential, asB. dorsalisdoes not infest papaya fruits at this stage; thus, authorities should permit entry of these papaya cultivars of less than 40% yellow ripening stage to quarantine-sensitive markets.

Field response of three Tephritid fruit flies to three food-based attractants and suppression of Bactrocera zonata (Saunders) using Mazoferm–Spinosad in guava ecosystem in Sudan

2021 ◽  
Author(s):  
Mohammed E. E. Mahmoud ◽  
Mohammed ◽  
Fathya M. Khamis and ◽  
Sunday Ekesi ◽  
Keyword(s):  
Fruits And Vegetables ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Economic Losses ◽  
Horticultural Crops ◽  
Torula Yeast ◽  
Bactrocera Zonata ◽  
Male Annihilation ◽  
Environmentally Friendly Approach ◽  
Tephritid Fruit Flies

Abstract Fruit flies of the genus Bactrocera are the most damaging pests of horticultural crops, leading to severe economic losses hindered exportation. Bactrocera dorsalis (Hendel) and Bactrocera zonata (Saunders) were reported in Sudan in 2005 and 2011 respectively affecting most of the fruits and vegetables in Sudan threatening income of poor farmers. Only Male Annihilation Technique (MAT) is applied in Sudan to manage the two Bactrocera species. A filed experiment was conducted to evaluate the response of B. dorsalis, B. zonata and Zeugodacus cucurbitae to three food-based attractants using McPhail traps in two sites in Gezira state, Sudan. Also, other trial was undertaken to determine the effect of spray of Mazoferm and Spinosad combination to control B. zonata. The results showed that food-based attractants lured both sexes of the above mentioned fruit flies and females represented (55-86%). At the first site, B. zonata responded in high numbers to Mazoferm followed by Torula yeast and GF-120 respectively while it responded equally to the Mazoferm and Torula yeast in the second site. B. dorsalis responded positively to Mazoferm followed by Torula yeast and GF-120 while Z. cucurbitae was attracted to Mazoferm, GF-120 and Torula for each attractant respectively. Spray of Mazoferm combined with Spinosad significantly reduced population of B. zonata (FTD) population and suppressed infestation level of guava fruits (fruit flies/Kg of fruits) when compared to unsprayed orchard. Bait Application Technique is an environmentally friendly approach that reduces infestation levels, lessen contamination and safeguard produce.

scholarly journals Impact of abiotic factors on population dynamics of Bactrocera dorsalis Hendel and Bactrocera zonata (Saunders) at different ecological zones in NW Plains of India

2021 ◽  
Vol 22 (3) ◽  
pp. 250-257
Author(s):  
REENA ◽  
AMANDEEP KAUR ◽  
MAHENDER SINGH ◽  
BHAV KUMAR SINHA ◽  
ANIL KUMAR ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Fruits And Vegetables ◽  
Abiotic Factors ◽  
Economic Loss ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
North West ◽  
Ecological Zones ◽  
Bactrocera Zonata ◽  
Trap Catches

In India, fruit flies have been identified as one of the ten most serious problems of agriculture because of their polyphagous nature and the huge economic loss it causes to the fruits and vegetables (2.5 to 100%). Study was carried to determine the population dynamics of fruit flies (Bactrocera dorsalis and Bactrocera zonata) and establish the correlation with abiotic factors in guava and citrus fruits crops in two agro ecological zones of North West India. Fruit flies population was recorded by installation of pheromone traps in different fruit crop ecosystems during the fruiting season of this zone. The data of trap catches of both the locations was correlated with the significantly influencing abiotic parameters. The peak fruit fly incidence varied between 31st to 34th standard weeks in guava ecosystem at both the locations. In citrus ecosystem, the incidence was highest during 35th and 39th standard weeks coinciding with the ripening of the fruits. At both the locations, trap catches of guava ecosystem were positively correlated with all the weather parameters, while under citrus ecosystem minimum temperature and rainfall showed negative correlation, but relative humidity was positively correlated. Information may be utilized in targeting suitable pest management techniques for respective zones.

scholarly journals Effects of Entomopathogenic Fungi on the Biology of Spodoptera litura (Lepidoptera: Noctuidae) and its Reduviid Predator, Rhynocoris marginatus (Heteroptera: Reduviidae)

2019 ◽  
Vol 11 ◽  
pp. 117954331986711 ◽  
Author(s):  
Muhammad Irfan Ullah ◽  
Nimra Altaf ◽  
Muhammad Afzal ◽  
Muhammad Arshad ◽  
Naunain Mehmood ◽  
...  
Keyword(s):  
Survival Rate ◽  
Entomopathogenic Fungi ◽  
Spodoptera Litura ◽  
Consumption Rate ◽  
Larval Mortality ◽  
Adult Emergence ◽  
Lower Efficiency ◽  
Significant Difference ◽  
Lepidoptera Noctuidae ◽  
Infected Prey

Entomopathogenic fungi (EPFs), Isaria fumosorosea and Beauveria bassiana, are efficient biological agents in the management of multiple arthropod pests. In this study, the effects of both EPF species on various life stages of Spodoptera litura (F.) (Lepidoptera: Noctuidae) and its natural enemy Rhynocoris marginatus (Fab.) (Hemiptera: Reduviidae) were determined under laboratory conditions. I. fumosorosea significantly ( P < .05) reduced the growth rate of the third and fourth instar larvae of S. litura. For relative consumption rate (RCR), the maximum impact was recorded for I. fumosorosea, which reduced the RCR of the larvae. The larvae of S. litura treated with I. fumosorosea showed significantly lower efficiency of conversion of ingested food (ECI) and the larval mortality rate (58.0%) was also higher compared with B. bassiana (33.3%). Similarly, I. fumosorosea had a significant effect on the pupal formation of S. litura; however, no significant effect was found on adult emergence percentage. To determine the effect of EPF-infected prey on the adult predator, their handling time, predatory rate, consumption rate, and the survival rate were recorded. No significant effect of EPF species on the predation rate was found. Furthermore, no significant difference was found in the survival rate of predators fed on either EPF-infected prey or healthy larvae. The interaction of these EPFs with a reduviid predator suggested that both EPF species, especially I. fumosorosea, could be used together with the predator to boost the biological control of S. litura in commercial crops.

scholarly journals Modified Atmosphere Does Not Reduce the Efficacy of Phytosanitary Irradiation Doses Recommended for Tephritid Fruit Flies

Insects ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 371 ◽  
Author(s):  
Vanessa S. Dias ◽  
Guy J. Hallman ◽  
Olga Y. Martínez-Barrera ◽  
Nick V. Hurtado ◽  
Amanda A. S. Cardoso ◽  
...  
Keyword(s):  
Adult Emergence ◽  
Fruit Fly ◽  
Agricultural Trade ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Modified Atmosphere ◽  
Radiation Doses ◽  
Low Oxygen ◽  
Oxygen Levels ◽  
Tephritid Fruit Flies

Phytosanitary irradiation (PI) has been successfully used to disinfest fresh commodities and facilitate international agricultural trade. Critical aspects that may reduce PI efficacy must be considered to ensure the consistency and effectiveness of approved treatment schedules. One factor that can potentially reduce PI efficacy is irradiation under low oxygen conditions. This factor is particularly important because storage and packaging of horticultural commodities under low oxygen levels constitute practices widely used to preserve their quality and extend their shelf life. Hence, international organizations and regulatory agencies have considered the uncertainties regarding the efficacy of PI doses for insects infesting fresh commodities stored under low oxygen levels as a rationale for restricting PI application under modified atmosphere. Our research examines the extent to which low oxygen treatments can reduce the efficacy of phytosanitary irradiation for tephritids naturally infesting fruits. The effects of normoxia (21% O2), hypoxia (~5% O2), and severe hypoxia (< 0.5% O2) on radiation sensitivity of third instars of Anastrepha fraterculus (sensu lato), A. ludens (Loew), Bactrocera dorsalis (Hendel), and Ceratitis capitata (Wiedemann) were evaluated and compared at several gamma radiation doses. Our findings suggest that, compared to normoxia, hypoxic and severe-hypoxic conditioning before and during irradiation can increase adult emergence and contribute to advancement of larval development of tephritid fruit flies only at low radiation doses that are not used as phytosanitary treatments. With phytosanitary irradiation doses approved internationally for several tephritids, low oxygen treatments applied before and during irradiation did not increase the emergence rates of any fruit fly species evaluated, and all treated insects died as coarctate larvae. Thus, the findings of our research support a re-evaluation of restrictions related to phytosanitary irradiation application under modified atmospheres targeting tephritid fruit flies.

scholarly journals Efficacy of Metarhizium anisopliae and Bacillus thuringiensis against tomato leafminer Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)

2019 ◽  
Vol 7 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Spyridon Mantzoukas ◽  
Nikoleta-Kleio Denaxa ◽  
Ioannis Lagogiannis
Keyword(s):  
Single Dose ◽  
Bacillus Thuringiensis ◽  
Dose Rate ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Larval Mortality ◽  
Adult Emergence ◽  
Biocontrol Agents ◽  
Tuta Absoluta ◽  
Combined Application

Susceptibility of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) populations to Metarhizium anisopliae and Bacillus thuringiensis (Bt) were evaluated under laboratory conditions. T. absoluta larvae were treated either individually or in combination with a single dose rate of B. thuringiensis (0.5 μL/L) and three conidial suspensions viz. 1x104, 1x106 and 1x108 spores/mL of M. anisopliae. Larval mortality, pupation, adult emergence, mycosis and sporulation varied depending on the application of different quantities of M. anisopliae alone and in combination with B. thuringiensis. Maximum mortality (100%) was achieved in 2nd instar larvae when M. anisopliae (1x108 spores/mL) and B. thuringiensis (0.5µL/L) were applied synergistically,while 4th instar larvae recorded a 95.45% mortality. Compared with the untreated checks, mortality, pupation and adult emergence of both 2nd and 4th instar larvae were significantly reduced with the combined application of M. anisopliae (1x108 spores/mL) and B. thuringiensis (0.5µL/L). Μycosis was most prevalent on 2nd and 4th instar larvae, exceeding 88 and 80% respectively,after exposure to 1×104 conidia/mL. The results indicate that the entomopathogenic fungi and the insecticidal protein produced by B. thuringiensis can be used in combination as biocontrol agents for the management of T. absoluta.

A weekly study on Bactrocera zonata S. and Bactrocera dorsalis H. (Diptera: Tephritidae) against methyl eugenol, raspberry essence and GF-120 in persimmon orchards from Kohat, Pakistan

2017 ◽  
Author(s):  
Uzair Ahmad ◽  
Unab Begum
Keyword(s):  
Population Density ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Methyl Eugenol ◽  
Population Trend ◽  
Bactrocera Dorsalis ◽  
Bactrocera Zonata ◽  
Number Of Males ◽  
Maximum Population Density ◽  
Over Time

A study was conducted to find out the efficacy of the lures viz methyl eugenol, raspberry essence and GF-120 in baited fruit fly traps at persimmon orchards in Kohat district, KPK. Three treatments: T1 (Methyl eugenol), T2 (Raspberry essence) and T3 (Gf-120) with nine replications treatment-1 were installed. Results revealed highest population density for fruit flies in T1 (382), following T3 (197.2) while the least population density was revealed in T2 (23.6). Population trend over time showed maximum population density for fruit flies at week 1, following week 2, week 4, week 3 and week 5. The number of fruit flies were observed with highest average number of Bactrocera zonata (700) in T1 (week 1), whereas Bactrocera dorsalis (250) in T1 (week 1), while the minimum average number of 08 in T2 (week 5) and 01 in T2 (week 5) was recorded for B. zonata and B. dorsalis, respectively. Maximum average numbers of males were recorded 950 in T1 (week 1), whereas the minimum average number of males revealed 09 in T2 (week 5). Average population density of fruit flies (gender) revealed 200.93 (male) while it revealed 0.0 for females.

scholarly journals First Record of an Invasive Fruit Fly Belonging to Bactrocera dorsalis Complex (Diptera: Tephritidae) in Europe

Insects ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 182 ◽  
Author(s):  
Francesco Nugnes ◽  
Elia Russo ◽  
Gennaro Viggiani ◽  
Umberto Bernardo
Keyword(s):  
Fruit Trees ◽  
Mediterranean Area ◽  
First Record ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Integrative Approach ◽  
Morphological Identification ◽  
Campania Region ◽  
Mediterranean Countries ◽  
Bactrocera Zonata

Emerging pests are increasingly threatening fruit orchard health across the Mediterranean area. Tephritidae, representing serious threats for Europe, are numerous, and the fruit flies Bactrocera zonata and those belonging to Bactrocera dorsalis complex are among the most alarming species. These species are highly polyphagous and B. zonata has already spread to some Mediterranean countries. Due to these ongoing threats, in the Campania Region (southern Italy), a survey with traps and infested fruits analysis was performed with the aim of detecting the presence of species of Bactrocera dorsalis complex. In two mixed fruit-trees fields, some adults belonging to a species of Bactrocera were captured in traps baited with the highly attractive male lure (methyl eugenol). They were distinguished from similar-looking Bactrocera spp. by morphological and molecular comparative analyses. Considering the existing morphological keys, specimens were tentatively identified as B. dorsalis but molecular characterization with COI split them into two clades. Some specimens were grouped with B. dorsalis similar to B. kandiensis and B. kandiensis and others in a clade including B. dorsalis and B. invadens (syn. B. dorsalis). ITS1 sequences instead confirmed morphological identification. The integrative approach allowed identifying all the specimens collected as belonging to the B. dorsalis complex. This finding represents the first field interception in Europe of a member of one of the most dangerous groups of fruit flies.

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