scholarly journals The Effectiveness of Biostimulation, Bioaugmentation and Sorption-Biological Treatment of Soil Contaminated with Petroleum Products in the Russian Subarctic

2021 ◽  
Vol 9 (8) ◽  
pp. 1722
Author(s):  
Vladimir A. Myazin ◽  
Maria V. Korneykova ◽  
Alexandra A. Chaporgina ◽  
Nadezhda V. Fokina ◽  
Galina K. Vasilyeva

The effectiveness of different bioremediation methods (biostimulation, bioaugmentation, the sorption-biological method) for the restoration of soil contaminated with petroleum products in the Russian Subarctic has been studied. The object of the study includes soil contaminated for 20 years with petroleum products. By laboratory experiment, we established five types of microfungi that most intensively decompose petroleum hydrocarbons: Penicillium canescens st. 1, Penicillium simplicissimum st. 1, Penicillum commune, Penicillium ochrochloron, and Penicillium restrictum. One day after the start of the experiment, 6 to 18% of the hydrocarbons decomposed: at 3 days, this was 16 to 49%; at 7 days, 40 to 73%; and at 10 days, 71 to 87%. Penicillium commune exhibited the greatest degrading activity throughout the experiment. For soils of light granulometric composition with a low content of organic matter, a more effective method of bioremediation is sorption-biological treatment using peat or granulated activated carbon: the content of hydrocarbons decreased by an average of 65%, which is 2.5 times more effective than without treatment. The sorbent not only binds hydrocarbons and their toxic metabolites but is also a carrier for hydrocarbon-oxidizing microorganisms and prevents nutrient leaching from the soil. High efficiency was noted due to the biostimulation of the native hydrocarbon-oxidizing microfungi and bacteria by mineral fertilizers and liming. An increase in the number of microfungi, bacteria and dehydrogenase activity indicate the presence of a certain microbial potential of the soil and the ability of the hydrocarbons to produce biochemical oxidation. The use of the considered methods of bioremediation will improve the ecological state of the contaminated area and further the gradual restoration of biodiversity.

Author(s):  
Khalid Sayed ◽  
Lavania Baloo ◽  
Naresh Kumar Sharma

A crude oil spill is a common issue during offshore oil drilling, transport and transfer to onshore. Second, the production of petroleum refinery effluent is known to cause pollution due to its toxic effluent discharge. Sea habitats and onshore soil biota are affected by total petroleum hydrocarbons (TPH) as a pollutant in their natural environment. Crude oil pollution in seawater, estuaries and beaches requires an efficient process of cleaning. To remove crude oil pollutants from seawater, various physicochemical and biological treatment methods have been applied worldwide. A biological treatment method using bacteria, fungi and algae has recently gained a lot of attention due to its efficiency and lower cost. This review introduces various studies related to the bioremediation of crude oil, TPH and related petroleum products by bioaugmentation and biostimulation or both together. Bioremediation studies mentioned in this paper can be used for treatment such as emulsified residual spilled oil in seawater with floating oil spill containment booms as an enclosed basin such as a bioreactor, for petroleum hydrocarbons as a pollutant that will help environmental researchers solve these problems and completely clean-up oil spills in seawater.


2021 ◽  
Vol 37 (1) ◽  
pp. 54-68
Author(s):  
E.S. Lobakova ◽  
G.A. Dolnikova ◽  
E.A. Ivanova ◽  
D.A. Sanjieva ◽  
A.A. Burova ◽  
...  

It has been shown that the studied petroleum products (kerosene and gasoline) contain microflocules of heterogeneous microbial biofilms, the cells of which are integrated into a polymer matrix containing acidic polysaccharides. Thirteen bacterial strains were microbiologically isolated from petroleum products, and their taxonomy was identified by the 16S rRNA sequence. Kerosene was characterized by a diverse bacterial composition including the following genera: Sphingobacterium, Alcaligenes, Rhodococcus and Deinococcus, while gasoline bacterial community included only two genera: Bacillus and Paenibacillus. Representatives of the Deinococcus genera capable of growing on the hydrocarbons were isolated from fuels for the first time. The strains isolated from gasoline (Bacillus safensis Bi13 and Bacillus sp. Bi14) proved to be the most effective biodegraders of all n-alkanes, isoalkanes, cycloalkanes, alkenes and aromatic hydrocarbons, whereas the kerosene strain Rhodococcus erythropolis Bi6 effectively decomposed n-alkanes and trimethylbenzene. Both types of petroleum products contained hydrocarbon-oxidizing communities, some members of which were more active in the biodegradation of hydrocarbons, while others were capable of producing biosurfactants and had either emulsifying activity (Deinococcus sp. Bi7) or cell wall hydrophobicity (Sphingobacterium sp. Bi5 from kerosene; Bacillus pumilus Bi12 from gasoline) significantly higher than the average level. The indicated properties of the studied strains make them promising for use in bioremediation. biodegradation, petroleum products, hydrocarbon-oxidizing bacteria, bio-surfactants The work was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (topic no. 10.5422.2017/8.9.). Investigation of microbial potential in the use hydrocarbons was supported by the Russian Foundation for Basic Research (RFBR), contract no. 18-29-05067. Physicochemical research was performed within the framework of the state assignment to the TIPS RAS


1994 ◽  
Vol 30 (3) ◽  
pp. 21-28 ◽  
Author(s):  
Nyuk-Min Chong

Mono- and Di- hydroxyl amines are used in the desulfuration processes for refined petroleum products. The refinery wastewater treatment plant may be shocked by amine laden wastewater periodically, bringing operation difficulties to the biological treatment units. Data on the treatability, shock load behaviour and on long term system stability of biological treatment of amines are therefore required. Shake-flask test results showed that pure diethanol amine and diisopropanol amines have characteristics of persistent compounds. Each of the two compounds has a prolonged lag time when first inoculated with indigenous activated sludge. Acclimated activated sludge in a continuous flow reactor treated a feed of ethanol amine with a 93 percent COD removal and a 98 percent nitrification, but the system was unstable because amine caused a bulking sludge. By physical retention of the activated sludge, 550 mg/l influent COD of amines was treated to m1 average 50 mg/l effluent COD. Sludge yield was approximately 0.26 mg MLSS per mg COD. The activated sludge system withstood a chm1ge of feed to a real refinery wastewater laden with the; amine. A mean cell residence time above five (5) days should be maintained for safe treatment of; amine.


1987 ◽  
Vol 1987 (1) ◽  
pp. 247-253
Author(s):  
Salah M. Al-Mazidi ◽  
Omar Samhan

ABSTRACT Since the discovery of oil in Kuwait, most oil-related activities have been located along the coastline 50 km south of Kuwait City. Other related industrial activities have been developed in this area apart from oil and petroleum products export in order to diversify the national sources of income. For these reasons, the potential for large oil spills in Kuwait's marine environment is highest along the south coast, where oil refineries and exporting facilities are located. An average of 219 barrels of oil were spilled annually between 1979 and 1985, and 2,100 gallons of dispersants were used in cleanup operations. The majority of incidents involved less than 5 barrels of oil and 500 gallons of dispersants. Incidents involving more than 100 barrels of oil and 5,000 gallons of dispersants were confined to the Sea Island and Mina Al-Ahmadi North and South Piers. This distribution undoubtedly affects the concentration of petroleum residues in various components of the marine environment, resulting in an increase in tar ball density along this coast, reaching a maximum at Ras Az-Zor, and significantly higher levels of vanadium and petroleum hydrocarbons in sediments and oysters collected south of Mina Al-Ahmadi. The objective of this paper is to report on the number, volume, and frequency distribution of oil spill incidents in Kuwait and the usage of dispersants in cleanup operations. Vanadium and petroleum hydrocarbon concentrations also are described as is the sensitivity of the southern coastal environment to oil spills. Recommendations have been made on how to conduct cleanup operations for any future oil spill incidents along the southern shoreline of Kuwait.


2020 ◽  
Vol 2 (10) ◽  
pp. 5-10
Author(s):  
Ishita Agrawal

It is widely known that petroleum hydrocarbons constitute one of the most hazardous pollutants that affect human and environmental health. The ongoing research on bioremediation with petroleum hydrocarbon-degrading bacteria has shown tremendous promise of the technology due to its advantages of high efficiency and eco-friendly nature. To this end, studies have been carried out to identify a large amount of bacterial species with petroleum hydrocarbon-degrading ability for applications in bioremediation. Here, we present a brief perspective of some of the notable advances in oil degrading bacteria and the remedial actions for decontamination of water and soil along with recovering the spilled materials at oil sites.


2022 ◽  
Vol 11 (6) ◽  
pp. 667-675
Author(s):  
Amina-Afaf MOUFFAK

Furfural is one of the petroleum products posing a potential danger to the environment and human health. However, the decontamination of these pollutants released into the environment is primarily governed by biodegra-dation processes. This study is based on biodegradation kinetics at increasing concentrations of furfural by natural mixed culture in order to assess the potential of this process in the elimination of furfural from petrochemical effluents from the ARZEW refinery. This biodegradation was measured through physicochemical parameters such as pH, electrical conductivity, con-centration of hydrocarbons, the chemical oxygen demand (COD), biochemi-cal oxygen demand (BOD5) and the concentration of furfural. The results obtained show at a concentration of 250ppm of injected furfural: a decrease in pH 4.9 and an increase in other parameters (conductivity 3450 μS.cm-1, HC 102 mg / l; furfural 210 ppm, COD 327mg / l, BOD5 98mgO2 / l. The study findings indicated that the injection of these effluents with concentrations greater than 180 ppm leads to values of pH, EC, HC, Furfural, COD, BOD5 which do not comply with direct discharge standards and disrupt biological treatment. The high levels of furfural not only cause a pollution problem but can also disrupt the functioning of bacteria at the biological treatment level. Therefore, dilution with the filtration wash water before switching to biologi-cal treatment is recommended in order to reduce the concentrations below 180 ppm.


Author(s):  
Audra Skaisgiriene ◽  
Pertas Vaitiekūnas ◽  
Vytenis Zabukas

The biological treatment method is widely used in Lithuania due to its high efficiency, low cost and suitability for treating various types of wastewater. After technological processes various mineral substances, such as chlorides and sulphates, get into water bodies. These substances are not removed from wastewater by the biological treatment method. The purpose of this study is to determine the concentrations of chlorides and sulphates, investigate the influence of enzyme preparations upon quality and energy consumption and assess energy changes in the trophic food chain during biological treatment. To analyse the mineral salt influence of the biooxidation process a series of laboratory tests was carried out: biochemical oxygen consumption (BOD), activated sludge concentration and other indexes. It is found experimentally that a 400 mg/1 concentration of chlorides disarranges the activity of microorganisms, and at a 700 mg/1 concentration active sludge is no longer suitable for biological treatment. Also, we investigated the effect of a ferment preparation “Roebic” on effluence of sewage treatment. In a tank with an enzyme the total amount of energy after treatment increases by 5–30 %. It is determined by calculating the accumulation of energy in an active‐sludge system that an enzyme preparation increases the energy levels of the system.


Author(s):  
Hasanova Aynur Oruj, Et. al.

The experience of developed countries in the field of agriculture and research results show that high efficiency from mineral fertilizers can be obtained on fertile soils. That is, the soil should contain a large amount of organic substances, microelements, beneficial microorganisms, various enzymes that activate microbiological processes, normalize and optimize the nutritional, thermal and water-physical properties of the soil. This can be achieved by regularly applying crop residues and organic fertilizers to the soil. Siderata - green fertilizers - are of great importance for increasing soil fertility.


Author(s):  
Đurđa Kerkez ◽  
◽  
Milena Bečelić-Tomin ◽  
Gordana Pucar Milidrag ◽  
Vesna Gvoić ◽  
...  

Synthetic dyes are widely used in textile, printing, leather tanning, cosmetic, drug and food processing industries. The printing and dyeing industry is considered as one of the most polluting industrial sectors. The printing process is very versatile and includes printing on paper as well as printing on textile, plastic and other materials. After the printing process is completed, various chemicals such as ethers, alcohols, phenols, aldehydes, ketones, benzene, and esters are used in the cleaning procedure. Resulting wastewater often contains a variety of solvents, surfactants, dyes, and other chemicals, thus greatly increasing the difficulty of wastewater treatment. Improper discharge of printing and dyeing wastewater into water bodies will have several effect, beginning with aesthetical issues followed by destruction of the aqueous ecosystem due to light attenuation, oxygen consumption and toxicity effects. Therefore, it is very important to find out and optimize printing and dying wastewater treatment techniques. Processes for dye removal from wastewater can be physical, chemical, biological and more recently hybrid treatments. Physical processes such as adsorption, based on mass transfer mechanism, are commonly used method mainly due to ease of operation and high efficiency. Chemical processes including coagulation and flocculation, advanced oxidation processes and electrochemical treatment are usually more expensive due t chemicals use, equipment requirements and electrical energy consumption. However, these techniques are destructive and may lead to total mineralization of dye molecules and accompanying pollutants. Biological treatment is a low-cost and environmentally friendly process that produces less sludge. This method has significant advantages but dye molecules are less prone to this kind of treatment as they are made to be stable and reluctant. So, the adjustment and optimization of biological treatment, for dye removal, is an ongoing field of research. In recent studies hybrid processes are gaining more attention, combining different techniques. Integrating treatments, as a cost-saving and time-saving process, can represent optimal solution for printing wastewater treatment.


Author(s):  
Alexis Nzila

Contamination of the environment by petroleum products is a growing concern worldwide, and strategies to remove these contaminants have been evaluated. One of these strategies is biodegradation, which consists of the use of microorganisms. Biodegradation is significantly improved by increasing the temperature of the medium, thus, the use of thermophiles, microbes that thrive in high-temperature environments, will render this process more efficient. For instance, various thermophilic enzymes have been used in industrial biotechnology because of their unique catalytic properties. Biodegradation has been extensively studied in the context of mesophilic microbes, and the mechanisms of biodegradation of aliphatic and aromatic petroleum hydrocarbons have been elucidated. However, in comparison, little work has been carried out on the biodegradation of petroleum hydrocarbons by thermophiles. In this paper, a detailed review of the degradation of petroleum hydrocarbons (both aliphatic and aromatic) by thermophiles was carried out. This work has identified the characteristics of thermophiles, and unraveled specific catabolic pathways of petroleum products that are only found with thermophiles. Gaps that limit our understanding of the activity of these microbes have also been highlighted, and, finally, different strategies that can be used to improve the efficiency of degradation of petroleum hydrocarbons by thermophiles were proposed.


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