scholarly journals The Impact of the Inoculation of Phosphate-Solubilizing Bacteria Pantoea agglomerans on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil

2021 ◽  
Vol 9 (8) ◽  
pp. 1661
Author(s):  
Ilhem Saadouli ◽  
Amor Mosbah ◽  
Raoudha Ferjani ◽  
Panagiota Stathopoulou ◽  
Ioannis Galiatsatos ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Pantoea Agglomerans ◽  
Available P ◽  
Arid Soil ◽  
Phosphate Availability ◽  
Phosphate Solubilizing ◽  
The Impact ◽  
Semi Arid

The bacterial genus Pantoea has been widely evaluated as promising bacteria to increase phosphorus (P) availability in soil. The aim of this study was to characterize the phosphate solubilizing (PS) activity of a Pantoea agglomerans strain and to evaluate the impact of its application in a semi-arid soil on phosphate availability and structure of the bacterial communities as a whole. An incubation experiment under close-to-natural soil environmental conditions was conducted for 15 days at 30 °C. High-throughput sequencing of the bacterial 16S rRNA gene was used to characterize and to compare the bacterial community structure of P. agglomerans-inoculated soil with non-inoculated control. Furthermore, a qPCR-based method was developed for detection and quantification of the functional genes related to the expression of mineral phosphate solubilization (MPS) phenotype in P. agglomerans. The results showed that in vitro solubilization of Ca3(PO4)2 by P. agglomerans strain was very efficient (980 mg/L), and it was associated with a drop in pH due to the secretion of gluconic acid; these changes were concomitant with the detection of gdh and pqqC genes. Moreover, P. agglomerans inoculum application significantly increased the content of available P in semi-arid soil by 69%. Metagenomic analyses showed that P. agglomerans treatment modified the overall edaphic bacterial community, significantly impacting its structure and composition. In particular, during P. agglomerans inoculation the relative abundance of bacteria belonging to Firmicutes (mainly Bacilli class) significantly increased, whereas the abundance of Actinobacteria together with Acidobacteria and Chloroflexi phyla decreased. Furthermore, genera known for their phosphate solubilizing activity, such as Aneurinibacillus, Lysinibacillus, Enterococcus, and Pontibacter, were exclusively detected in P. agglomerans-treated soil. Pearson’s correlation analysis revealed that changes in soil bacterial community composition were closely affected by soil characteristics, such as pH and available P. This study explores the effect of the inoculation of P. agglomerans on the bacterial community structure of a semi-arid soil. The effectiveness in improving the phosphate availability and modification in soil bacterial community suggested that P. agglomerans represent a promising environmental-friendly biofertilizer in arid and semi-arid ecosystems.

Tu1716 Evaluation of the Impact of Different Commercially Available DNA Extraction Kits and Laboratories for Assessing Bacterial Community Structure in Fecal Samples - Implications for IBD Research

2013 ◽  
Vol 144 (5) ◽  
pp. S-829
Author(s):  
Nicholas A. Kennedy ◽  
Alan Walker ◽  
UK IBD Microbiota Consortia ◽  
UK IBD Genetics Consortia ◽  
Susan H. Berry ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Dna Extraction ◽  
Bacterial Community Structure ◽  
Fecal Samples ◽  
The Impact

scholarly journals The impact of host rock geochemistry on bacterial community structure in oligotrophic cave environments

2007 ◽  
Vol 36 (2) ◽  
pp. 93-104 ◽  
Author(s):  
Hazel Barton ◽  
Nicholas Taylor ◽  
Michael Kreate ◽  
Austin Springer ◽  
Stuart Oehrle ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Host Rock ◽  
Bacterial Community Structure ◽  
The Impact

scholarly journals Responses of Soil Organic Carbon Sequestration Potential and Bacterial Community Structure in Moso Bamboo Plantations to Different Management Strategies in Subtropical China

Forests ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 657 ◽  
Author(s):  
Chuanbao Yang ◽  
Zheke Zhong ◽  
Xiaoping Zhang ◽  
Fangyuan Bian ◽  
Xuhua Du
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Management Strategies ◽  
Moso Bamboo ◽  
Mineralization Rate ◽  
Bamboo Forest ◽  
C Mineralization ◽  
Sequestration Potential ◽  
The Impact

Moso bamboo is one of the fastest-growing plants in the world. The objective of this study was to investigate the impact of converting secondary broadleaf evergreen forests (CK) to Moso bamboo plantations, and the impact of different management strategies, including no disturbance (M0), extensive management (M1), and intensive management (M2), on the soil organic carbon (SOC) sequestration potential, and relevant characteristics of the soil bacterial community. Our results showed that, in comparison with CK, M0 and M1 had significantly higher SOC and recalcitrant organic materials (aliphatic and aromatic compounds), and a lower C mineralization rate, whereas M2 had the opposite effects. The conversion from CK to Moso bamboo plantation significantly decreased the relative abundance of Acidobacteria in both the topsoil and subsoil soil layers. Compared with CK, M0 led to the enrichment of bacteria such as Alphaproteobacteria, Chloroflexi, and Bacteroidetes, which are involved in the decomposition of organic matter and the formation of humus and are, therefore, potentially beneficial for increasing the SOC. Furthermore, the ratio of the microbial biomass C (MBC) to total organic C (TOC), C mineralization rate, and bacterial diversity increased from M0 to M2, i.e., with an increase in the disturbance intensity. These findings indicate that the conversion of secondary broadleaf forest to bamboo forest alter the soil bacterial community structure. Reducing disturbance in bamboo forest management strategies should be actively taken up to improve the SOC, and maintain sustainable development in the forest industry.

Bacterial community structure in the rhizosphere of three cactus species from semi-arid highlands in central Mexico

2012 ◽  
Vol 101 (4) ◽  
pp. 891-904 ◽  
Author(s):  
J. Félix Aguirre-Garrido ◽  
Daniel Montiel-Lugo ◽  
César Hernández-Rodríguez ◽  
Gloria Torres-Cortes ◽  
Vicenta Millán ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Central Mexico ◽  
Semi Arid

The influence of season, agricultural management, and soil properties on gross nitrogen transformations and bacterial community structure

Soil Research ◽  
2006 ◽  
Vol 44 (4) ◽  
pp. 453 ◽  
Author(s):  
W. R. Cookson ◽  
P. Marschner ◽  
I. M. Clark ◽  
N. Milton ◽  
M. N. Smirk ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Soil Temperature ◽  
Bacterial Community Structure ◽  
Dominant Factor ◽  
Organic N ◽  
N Fluxes ◽  
C And N ◽  
C And N Pools ◽  
Semi Arid

The aim of this study was to assess the influence of season, farm management (organic, biodynamic, integrated, and conventional), and soil chemical, physical, and biological properties on gross nitrogen (N) fluxes and bacterial community structure in the semi-arid region of Western Australia. Moisture availability was the dominant factor mediating microbial activity and carbon (C) and N cycling under this climate. In general, microbial biomass N, dissolved organic N, and potentially mineralisable N were greater in organic and biodynamic than integrated and conventional soil. Our results indicate that greater silt and clay content in organic and biodynamic soil may also partly explain these differences in soil N pools, rather than management alone. Although plant-available N (NH4+ + NO3–) was greater in conventional soil, this was largely the result of higher NO3– production. Multiple linear modelling indicated that soil temperature, moisture, soil textural classes, pH, electrical conductivity (EC), and C and N pools were important in predicting gross N fluxes. Redundancy analysis revealed that bacterial community structure, assessed by denaturing gradient gel electrophoresis of 16S rDNA, was correlated with C and N pools and fluxes, confirming links between bacterial structure and function. Bacterial community structure was also correlated with soil textural classes and soil temperature but not soil moisture. These results indicate that across this semi-arid landscape, soil bacterial communities are relatively resistant to water stress.

scholarly journals Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms

2017 ◽  
Vol 83 (10) ◽  
Author(s):  
Stephen M. Techtmann ◽  
Mobing Zhuang ◽  
Pablo Campo ◽  
Edith Holder ◽  
Michael Elk ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Microbial Communities ◽  
Bacterial Community Structure ◽  
Oil Spills ◽  
Content Type ◽  
Corexit 9500 ◽  
Oil Biodegradation ◽  
Degrading Bacteria ◽  
The Impact

ABSTRACT To better understand the impacts of Corexit 9500 on the structure and activity levels of hydrocarbon-degrading microbial communities, we analyzed next-generation 16S rRNA gene sequencing libraries of hydrocarbon enrichments grown at 5 and 25°C using both DNA and RNA extracts as the sequencing templates. Oil biodegradation patterns in both 5 and 25°C enrichments were consistent with those reported in the literature (i.e., aliphatics were degraded faster than aromatics). Slight increases in biodegradation were observed in the presence of Corexit at both temperatures. Differences in community structure were observed between treatment conditions in the DNA-based libraries. The 25°C consortia were dominated by Vibrio, Idiomarina, Marinobacter, Alcanivorax, and Thalassospira species, while the 5°C consortia were dominated by several species of the genera Flavobacterium, Alcanivorax, and Oleispira. Most of these genera have been linked to hydrocarbon degradation and have been observed after oil spills. Colwellia and Cycloclasticus, known aromatic degraders, were also found in these enrichments. The addition of Corexit did not have an effect on the active bacterial community structure of the 5°C consortia, while at 25°C, a decrease in the relative abundance of Marinobacter was observed. At 25°C, Thalassospira, Marinobacter, and Idiomarina were present at higher relative abundances in the RNA than DNA libraries, suggesting that they were active in degradation. Similarly, Oleispira was greatly stimulated by the addition of oil at 5°C. IMPORTANCE While dispersants such as Corexit 9500 can be used to treat oil spills, there is still debate on the effectiveness on enhancing oil biodegradation and its potential toxic effect on oil-degrading microbial communities. The results of this study provide some insights on the microbial dynamics of hydrocarbon-degrading bacterial populations in the presence of Corexit 9500. Operational taxonomic unit (OTU) analyses indicated that several OTUs were inhibited by the addition of Corexit. Conversely, a number of OTUs were stimulated by the addition of the dispersant, many of which were identified as known hydrocarbon-degrading bacteria. The results highlight the value of using RNA-based methods to further understand the impact of dispersant on the overall activity of different hydrocarbon-degrading bacterial groups.

scholarly journals Shift of anammox bacterial community structure along the Pearl Estuary and the impact of environmental factors

2015 ◽  
Vol 120 (4) ◽  
pp. 2869-2883 ◽  
Author(s):  
Bingbing Fu ◽  
Jiwen Liu ◽  
Hongmei Yang ◽  
Ting Chang Hsu ◽  
Biyan He ◽  
...  
Keyword(s):  
Community Structure ◽  
Environmental Factors ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
The Impact
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