scholarly journals Salmonella Bacterin Vaccination Decreases Shedding and Colonization of Salmonella Typhimurium in Pigs

2021 ◽  
Vol 9 (6) ◽  
pp. 1163
Author(s):  
Eduarda Alexandra Gonçalves de Oliveira Moura ◽  
Daniela Gomes da Silva ◽  
Caio Henrique Turco ◽  
Thainara Vitoria Carnevalli Sanches ◽  
Gabriel Yuri Storino ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Blood Count ◽  
Control Strategies ◽  
Control Group ◽  
Blood Samples ◽  
Commercial Vaccine ◽  
Serum Igg ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Since the occurrence of swine salmonellosis has increased over time and control strategies other than biosecurity are highly recommended, the present study aimed to evaluate the efficacy of vaccination with Salmonella Choleraesuis and Salmonella Typhimurium bacterins in pigs. Two experimental groups were formed: G1, animals immunized with two doses of a commercial vaccine (n = 20); G2, control group (n = 20). After vaccination, all pigs were orally challenged (D0) with 108 CFU of Salmonella Typhimurium and evaluated for 40 days. Every 10 days after D0, five piglets from each experimental group were euthanized and submitted to the necroscopic examination, when organ samples were collected. Blood samples and rectal swabs were collected before the first dose of the vaccine (D−42), before the second dose (D−21), before the challenge (D0), and thereafter, every three days until D39. Blood count, serum IgG measurement by ELISA, and the excretion of Salmonella Typhimurium in feces were evaluated. While the results from blood count and serum IgG concentration did not differ, the detection and excretion of Salmonella between G1 and G2 differed (p < 0.05). Therefore, it was observed that this vaccine partially protected the animals against experimental infection with Salmonella Typhimurium, reducing the excretion of bacteria in feces.

Colonization of Broiler Chicks by Salmonella Typhimurium Definitive Phage Type 104

2001 ◽  
Vol 64 (11) ◽  
pp. 1698-1704 ◽  
Author(s):  
PAULA J. FEDORKA-CRAY ◽  
SCOTT R. LADELY ◽  
J. STAN BAILEY ◽  
NORMAN J. STERN
Keyword(s):  
Salmonella Typhimurium ◽  
Broiler Chickens ◽  
Phage Type ◽  
Control Group ◽  
Broiler Chicks ◽  
Control Groups ◽  
Salmonella Spp ◽  
And Control ◽  
Salmonella Senftenberg ◽  
Over Time

The prevalence of an antibiotic-resistant strain of Salmonella Typhimurium definitive phage type 104 (DT104) has increased dramatically in recent years resulting in increased morbidity and mortality in both animals and humans. Colonization and shedding of Salmonella Typhimurium DT104 was studied in broiler chickens in two trials. In trial 1, 180 day-of-hatch chicks (n = 60 per group, n = 30 per replicate) were challenged with 106 CFU DT104 (wild-type isolate from poultry) or were commingled with a seeder chick challenged with 106 CFU DT104. In trial 2, 360 day-of-hatch chicks (n = 120 per treatment, n = 30 per rep) were divided into three groups. Chicks in the susceptible group were commingled with two seeder chicks that were orally challenged with 107 CFU/bird of a pan-sensitive strain of Salmonella Typhimurium DT104. Chicks in the resistant group were commingled with two seeder chicks that were orally challenged with 107 CFU/bird DT104 used in trial 1. For both trials, a control group was not exposed to DT104, composite fecal samples were evaluated twice weekly for levels of Salmonella shedding and 20 chicks per group were necropsied weekly and their cecal contents were cultured. At hatch all groups were colonized with naturally occurring Salmonella Senftenberg and Salmonella Mbandaka (trial 1) or Salmonella Senftenberg and Salmonella Ohio (trial 2) prior to exposure to DT104. Throughout the study, the level of Salmonella spp. shedding in feces (trial 1 means 3.1, 2.9, and 3.0 log10 CFU per g feces for challenged, seeder, and control groups, respectively) or ceca (trial 2 means 2.9, 2.9, and 2.5 log10 CFU per g ceca for resistant, susceptible, and control groups, respectively)did not differ among groups. In trial 1, colonization of DT104 remained constant at higher levels in the challenged group (mean 87%, P &lt; 0.01), increased over time in the seeder group (10 to 50%, P &lt; 0.02) and was not recovered from the control chicks. Salmonella Mbandaka colonization remained steady within each group with challenge and seeder groups maintaining higher levels of colonization than the control group. Salmonella Senftenberg colonization levels tended to decline (P = .058) over time in the challenged group (20 to 0%) and significantly decreased (P &lt; 0.01) over time for both the seeder (80 to 0%) and control chicks (85 to 10%). In trial 2, the percentage of chicks colonized with susceptible DT104 declined (r = 0.90, P &lt; 0.05) over the course of the trial from 45 to 0%, while recovery of the resistant DT104 persisted at a mean percentage of 27%. DT104 was not recovered from the control chicks. Salmonella Ohio colonization levels tended to decline (r = 0.79, P &gt; 0.05) over time in the control group (75 to 20%) and significantly decreased (P &lt; 0.05) over time in both susceptible and resistant groups (40 to 10%, r = 0.82 and 55 to 5%, r = 0.85, respectively). Salmonella Senftenberg was recovered from the control group at low frequency throughout the trial and was not recovered from the other groups. For either trial, no apparent affect on morbidity or mortality was observed. Introduction of DT104 by commingling may induce colonization resulting in persistent high levels of shedding in flocks simultaneously with other Salmonella species.

scholarly journals Evaluation of stress in laboratory rabbits used for teaching purposes

2019 ◽  
Vol 88 (2) ◽  
pp. 249-255
Author(s):  
Monika Urbanová ◽  
Eva Kramářová ◽  
Jan Chloupek ◽  
Martina Najmanová
Keyword(s):  
Oryctolagus Cuniculus ◽  
Control Group ◽  
Health Examination ◽  
Pharmaceutical Sciences ◽  
Blood Samples ◽  
Habituation Process ◽  
Biochemical Indices ◽  
The University ◽  
And Control ◽  
Experimental Group

This study was intended as a contribution to the argument about possible suffering of animals used for demonstrative purposes during teaching at universities. Pharmacology lectures at the University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic were selected for this experiment. The study focused on stress assessment using haematological and biochemical indices. Blood samples were drawn from 20 rabbits (Oryctolagus cuniculus f. domesticus L.) 10 days after arrival at the institute (before the first practical lesson), and then in the 7th and 12th weeks of the semester with practical lessons. The haematological and biochemical indices were compared between the experimental group (n = 10) used for practical demonstrations and the control group (n = 10) which was kept in its hutch. Practical lessons included manipulation with rabbits, health examination, topical or total application and observation of the drugs’ effects. All the acts were carried out by students. Significant changes were detected in some of haematological (erythrocytes, haemoglobin, haematocrit) and biochemical (glucose, total protein, enzymes aspartate aminotransferase and lactate dehydrogenase) variables compared to the control group. The values obtained from the last blood sampling indicate a possible habituation process in the experimental group between the 8th and 12th week. The results were compared between the experimental and control group and also with the results of other studies with different stressors. In conclusion, the stress the rabbits used for teaching purposes at universities are exposed to, is tolerable.

scholarly journals The Effect of Ginger on Preventing Post-spinal Puncture Headache in Patients Undergoing Cesarean Section

2018 ◽  
Vol 7 (2) ◽  
pp. 204-210
Author(s):  
Banafsheh Mashak ◽  
Maryam Hashemnejad ◽  
Kourosh Kabir ◽  
Mansoureh Refaei ◽  
Sara Esmaelzadeh Saeieh ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Spinal Anesthesia ◽  
Repeated Measures ◽  
Control Group ◽  
Control Groups ◽  
Spinal Puncture ◽  
Comparison Results ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Objectives: Post-spinal puncture headache (PSPH) has constantly been one of the research priorities, especially in women undergoing cesarean section (C-section) and it is related to physical and psychological problems. The present study aimed to evaluate the effect of ginger on preventing PSPH in patients undergoing C-section. Materials and Methods: This clinical trial was conducted on 160 women undergoing C-section with spinal anesthesia, who were eligible to enter the study in the experimental and control groups. One ginger capsule (250 mg) was prescribed every 8 hours (TDS) to the experimental group 24 hours before the C-section. The prescription of ginger was continued half an hour after transfer to the post-partum ward until the PSPH onset. PSPH intensity was measured by using visual analog scale (VAS) pain scores at 6 timepoints (Time 1=30, Time 2=60, and Time 3=90 minutes vs. Time 4=3, Time 5 =6, and Time 6=12 hours) after C-section. No interventions were performed in the control group. The data were analyzed using the SPSS software by descriptive statistics and analytical tests were applied to determine the changes in PSPH intensity. Results: The comparison results of the mean score of PSPH intensity in the experimental and control groups indicated significant differences over time (P<0.05), except for the sixth time point (12 hours after C-section). In addition, the trend of changes at 6 timepoints based on the results of the repeated-measures test demonstrated that PSPH intensity significantly differed in the two groups over time (P<0.001). Conclusions: Based on the result, the oral prescription of ginger to women undergoing C-section under spinal anesthesia led to effective PSPH prevention at 5 time-points (30, 60, and 90 minutes, along with 3 and 6 hours). Further, the trend of the changes represented that the intensity of PSPH decreased in the experimental group over time. Therefore, ginger is suggested as a noninvasive and efficient method used for preventing PSPH.

scholarly journals Blood calcium and phosphorus concentrations in cows with left displaced abomasum

2009 ◽  
Vol 63 (5-6) ◽  
pp. 331-340
Author(s):  
Horea Samanc ◽  
Danijela Kirovski ◽  
Djordje Savic ◽  
Zeljko Sladojevic ◽  
Ivan Vujanac ◽  
...  
Keyword(s):  
Organic Phosphorus ◽  
High Yield ◽  
Control Group ◽  
Blood Calcium ◽  
Blood Samples ◽  
Average Value ◽  
Calcium And Phosphorus ◽  
And Control ◽  
Experimental Group ◽  
Abomasal Displacement

Abomasal displacement is one of the most important disorders of the digestive tract in high-yield dairy cows. The etiology and pathogenesis of its occurrence has not been fully clarified to this day. The work examines calcaemia and phosphataemia in cows during the antepartal and postpartal periods and their connection with the incidence of abomasal displacement. The experiment covered 30 cows in advanced stages of gravidity. Blood samples were taken two weeks before expected parturition and two weeks after calving. In all blood samples (taken antepartal and postpartal), concentrations of calcium and an organic phosphorus were determined using a commercial test package (Bio-Merieux). Following parturition, left displacement of the abomasums was established in seven (23.33 %) of the 30 cows covered by the experiment (experimental group). The remaining 23 cows in the experiment were clinically healthy, they were placed in a group and represented control animals. The average value of calcaemia for the control group of cows during the antepartal period was 2.72?0.25 mmol/l, and of phosphataemia 2.04?0.25 mmol/l. In the same cows postpartum, average calcaemia values were 2.46?0.22 mmol/l and phosphataemia 1.85?0.29 mmol/l. The average antepartal value for calcaemia in the experimental group of cows was 2.51?0.25 mmol/l, and for phosphataemia 1.73? 0.22 mmol/l. The average values for this group of cows postpartally was 2.13?0.31 mmol/l for calcaemia and 1.43?0.24 mmol/l for phosphataemia. The differences between the concentration values for calcium and phosphorus obtained postpartally between the experimental and control groups were statistically significant. .

scholarly journals THE EFFECT OF INTENSIVE DOGS INVASION BY DIROFILARIASIS ON THE MORPHOLOGICAL PICTURE OF BLOOD

2019 ◽  
pp. 238-241 ◽  
Author(s):  
Kabardiev ◽  
Bittirova ◽  
Bittirov ◽  
Kabardiev ◽  
Begieva ◽  
...  
Keyword(s):  
High Intensity ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Blood Samples ◽  
Hematological Profile ◽  
Morphological Picture ◽  
Morphological Composition ◽  
And Control ◽  
Experimental Group ◽  
Forearm Vein

New data were obtained when studying the effect of intensive dirofilariоsis invasion on the morphological composition of the blood corpus of infected dogs. Hematological studies were performed on 20 dogs infected intensively with microfilaria. Blood from dogs was taken from a forearm vein in test tubes with an anticoagulant. For research, 2 groups were formed: experimental and control. The experimental group (n=10) consisted of dogs that had microfilaria dirofilariasis in blood samples. The control group of dogs (n=10) was formed from agelminthous animals, in the blood of which there were no nematode microfilaria. Results. With intensive invasion of dirofilariоsis in blood samples of dogs, a decrease in the number of erythrocytes is observed by 3.03х1012/l, a decrease in hemoglobin concentration by 70.68 g/l. At the same time, the number of leukocytes in the hematological profile of experienced dogs increased by 14.68х109/l, and that of eosinophils by 497.44%, which indicates profound pathophysiological changes in the hematocrit. Conclusion With a high intensity of dirofilariasis in dogs, erythropenia, hypohemoglobinemia, leukocytosis, and eosinophilia appear strongly on the background of control.

scholarly journals EFFECT OF STAPHYLOCOCCUS AUREUS INFECTION ON THE ACTIVITIES OF THE ALKALINE PHOSPHATASE, ASPARTATE AND ALANINE TRANSFERASES IN THE BLOOD PLASMA OF RABBITS

2019 ◽  
Vol 17 (4) ◽  
pp. 334-339
Author(s):  
T. Mircheva Georgieva ◽  
Iv. Penchev Georgiev ◽  
N. Markov ◽  
I. Markov ◽  
N. Bozakova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Veterinary Medicine ◽  
Initial Level ◽  
Control Group ◽  
Blood Samples ◽  
Aureus Infection ◽  
Biochemical Analyzer ◽  
And Control ◽  
Experimental Group

The aim of this paper is to investigate the dynamics of the activities of alkaline phosphatase (AP), aspartate (ASAT) and alanine (ALAT) transferases in plasma of rabbits that are infected with Staphylococcus aureus (S. aureus). METHODS: The experiment was carried out with 12 male rabbits (White New Zealand), divided into two groups: experimental and control. Blood samples from each rabbit were taken before the euthanasia of v. auricularis externa as follows: at 3 months of age, coinciding with 0 hours before infection, and at 6, 24, 48, 72 hours and on days 7, 14 and 21 after S. aureus infection in sterile heparinized tubes. The AP, ASAT and ALAT were performed on a semi-automated biochemical analyzer, Section in Biochemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria. RESULTS: The results showed significant decreasing (P <0.05) of AP 48h after infection and on day 14 comparing to the control group(P<0,01), significant increasing of ALAT (P<0,01) comparingto the control group and pre-initial level and significant increasing of ASAT on day 14 and day 21 in the experimental group comparing initial level.

The Effect of Applying POSSE (Predict- Organize-Search-Summarize-Evaluate) on the Students’ Reading Comprehension

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Eka Rejeki Maha ◽  
Berlin Sibarani
Keyword(s):  
Reading Comprehension ◽  
Experimental Design ◽  
Null Hypothesis ◽  
Alternative Hypothesis ◽  
Choice Test ◽  
Control Group ◽  
Multiple Choice Test ◽  
Level Of Significance ◽  
And Control ◽  
Experimental Group

This study was aimed at finding out The Effect of Applying POSSE (Predict-Organize-Search-Summarize-Evaluate) Strategy on the Students’ Reading Comprehension. This study used the experimental design. The population of this study was the students of SMA Negeri 2 Medan. There were sixty students taken as the sample of the research. This study was conducted with two randomized groups namely experimental and control group. The experimental group was taught by applying POSSE strategy while control group was taught without applying POSSE strategy. The instrument of collecting the data was multiple choice test which consists of 40 items. To obtain the reliability of the test, the researcher used Kuder-Richardson (KR-21). The calculation shows the reliability was 0.81(high). The data were analyzed by using t-test. The calculation shows that t-observed (4.76) is higher than t-table (2.00) at the level of significance (α) 0.05 with the degree of freedom (df) 58. Therefore, the null hypothesis (Ho) is rejected and alternative hypothesis (Ha) is accepted. It means that there was a significant effect of applying POSSE strategy on the students’ reading comprehension. Keywords: POSSE Strategy, Reading Comprehension.

The Effect of Applying Learning Together Method on the Students’ Reading Comprehension

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Haryato Siregar ◽  
Elia Masa Ginting
Keyword(s):  
Reading Comprehension ◽  
Alternative Hypothesis ◽  
Choice Test ◽  
Control Group ◽  
Teaching Reading ◽  
Multiple Choice Test ◽  
Level Of Significance ◽  
And Control ◽  
Experimental Group ◽  
Learning Together

This study was aimed at finding out the effect of applying Learning Together Method on the students’ reading comprehension. This study used the experimental design. The population of this study was the students of SMP Negeri 1 Muara. There were sixty students of eighth class as the sample of the research. This study was conducted with two randomized groups namely experimental and control group. The experimental group was taught by applying Learning Together Method while control group was taught without applying Learning Together Method. The instrument of collecting the data was multiple choice test which consists of 20 items. To obtain the reliability of the test, the researcher used Kuder-Richardson (KR-21) formula. The calculation shows the reliability was 0.82(high). The data were analyzed by using t-test. The calculation shows that t-observed (4.9) is higher than t-table (2.00) at the level of significance (α) 0.05 with the degree of freedom (df) 58. Therefore, the null hypothesis (Ho) is rejected and alternative hypothesis (Ha) is accepted. It means that teaching reading comprehension by applying Learning Together Method significantly affect reading comprehension. Keywords: Learning Together Method, Reading Comprehension

THE EFFECT OF APPLYING ANIMAL CARTOON PICTURES ON STUDENTS’ ACHIEVEMENT IN WRITING NARRATIVE TEXT

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Hasanul Arifin Zul And Masitowarni Siregar
Keyword(s):  
T Test ◽  
Narrative Text ◽  
Control Group ◽  
First Year ◽  
Writing Achievement ◽  
Control Groups ◽  
Post Test ◽  
Written Test ◽  
And Control ◽  
Experimental Group

This thesis is focused on the investigation of the effect of applying animal cartoon pictures on students’ achievement in writing narrative text. This study aims to find whether applying animal cartoon pictures significantly affect the students’ writing achievement or not. The data in this study were obtained by administering a written test. The population was the 2015/2016 first year (grade XI) of SMA Swasta Nusantara Lubuk Pakam and 66 students were taken as the sample by using random sampling. The sample was divided into two groups, experimental and control groups. The experimental group was taught by applying animal cartoon pictures while the control group without animal cartoon pictures (x = lecturing). The data were taken the scores from the pre-test and post-test to both experimental and control groups. These data were analyzed by using t-test. The result of computing the t-test obviously showed that t-observed is higher than t-table (5.21 >1,67) with the degree of freedom 64 (df =N-2) at the level significance 0,05 one tail test. It showed that the application of animal cartoon pictures significantly affected the students of SMA Swasta Nusantara Lubuk Pakam achievement in writing narrative text.

THE EFFECT OF PEER REVIEW STRATEGY ON THE STUDENTS’ ACHIEVEMENT IN WRITING DESCRIPTIVE PARAGRAPH

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Sri Agus Murniasih And I Wayan Dirgeyasa
Keyword(s):  
Peer Review ◽  
Experimental Research ◽  
Research Method ◽  
Alternative Hypothesis ◽  
Control Group ◽  
Post Test ◽  
Class Viii ◽  
And Control ◽  
Essay Test ◽  
Experimental Group

This study was conducted to know the effect of applying Peer Review Strategy on the students’ achievement in writing descriptive paragraph. It was conducted by using experimental research method. This research design was conducted pre-test, post test in experimental and control group. The population of this research was the eight (VIII) grade students of SMP IT IQRA’ Medan. The sample of this research was taken by lottery technique. They were in class VIII-1 by experimental group and the class VIII-2 by control group. The experimental group was taught by using Peer Review Strategy while the control group was given no treatment. The instrument used to collect the data was writing essay test. The data were analyzed by using t-test formula. The result showed that the value of t-observed was higher than the value of t-table. (3,55 > 2,05 (a = 0.05)) with the degree of freedom (df) = 28. It means that there was a significant effect of applying Peer Review Strategy on the students’ achievement in writing descriptive paragraph. So, the alternative hypothesis (Ha) was accepted.

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