scholarly journals Bioreactor Co-Cultivation of High Lipid and Carotenoid Producing Yeast Rhodotorula kratochvilovae and Several Microalgae under Stress

2021 ◽  
Vol 9 (6) ◽  
pp. 1160
Author(s):  
Martin Szotkowski ◽  
Jiří Holub ◽  
Samuel Šimanský ◽  
Klára Hubačová ◽  
Pavlína Sikorová ◽  
...  
Keyword(s):  
Positive Impact ◽  
Small Scale ◽  
Biomass Growth ◽  
Media Composition ◽  
Yeast Strains ◽  
High Lipid ◽  
Algae Growth ◽  
Chlorophyll Production ◽  
Lipid Metabolites ◽  
Present Pilot Study

The co-cultivation of red yeasts and microalgae works with the idea of the natural transport of gases. The microalgae produce oxygen, which stimulates yeast growth, while CO2 produced by yeast is beneficial for algae growth. Both microorganisms can then produce lipids. The present pilot study aimed to evaluate the ability of selected microalgae and carotenogenic yeast strains to grow and metabolize in co-culture. The effect of media composition on growth and metabolic activity of red yeast strains was assessed simultaneously with microalgae mixotrophy. Cultivation was transferred from small-scale co-cultivation in Erlenmeyer flasks to aerated bottles with different inoculation ratios and, finally, to a 3L bioreactor. Among red yeasts, the strain R. kratochvilovae CCY 20-2-26 was selected because of the highest biomass production on BBM medium. Glycerol is a more suitable carbon source in the BBM medium and urea was proposed as a compromise. From the tested microalgae, Desmodesmus sp. were found as the most suitable for co-cultivations with R. kratochvilovae. In all co-cultures, linear biomass growth was found (144 h), and the yield was in the range of 8.78–11.12 g/L of dry biomass. Lipids increased to a final value of 29.62–31.61%. The FA profile was quite stable with the UFA portion at about 80%. Around 1.98–2.49 mg/g CDW of carotenoids with torularhodine as the major pigment were produced, ubiquinone production reached 5.41–6.09 mg/g, and ergosterol yield was 6.69 mg/g. Chlorophyll production was very low at 2.11 mg/g. Pilot experiments have confirmed that carotenogenic yeasts and microalgae are capable of symbiotic co-existence with a positive impact om biomass growth and lipid metabolites yields.

scholarly journals Development and Evaluation of an Ultrasonic Humidifier to Control Humidity in a Cold Storage Room for Postharvest Quality Management of Dates

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 949
Author(s):  
Maged Mohammed ◽  
Nashi Alqahtani ◽  
Hamadttu El-Shafie
Keyword(s):  
Quality Management ◽  
Cold Storage ◽  
Cooling System ◽  
Positive Impact ◽  
Droplet Diameter ◽  
Postharvest Quality ◽  
Main Element ◽  
Small Scale ◽  
Storage Room ◽  
Ultrasonic Humidifier

Dates are subjected to postharvest losses in quality and quantity caused by water loss, fermentation, insect infestation, and microbial spoilage during storage. Cold storage is the main element in the postharvest quality management used for fruit preservation. Although cold storage is used for dates, precision control of the relative humidity (RH) using ultrasonic applications is not used thus far, or it is applied to other fruits on a small scale. Therefore, we designed and constructed an ultrasonic humidifier (DUH) for RH control in the cold storage room (CSR) of dates. The optimum air velocity of 3 m s−1 at the outlets of the DUH ducts produced a mist amount of 6.8 kg h-1 with an average droplet diameter of 4.26 ± 1.43 µm at the applied voltage of 48 V and frequency of 2600 kHz of the transducers. The experimental validation was carried out by comparing a CSR controlled with the DUH with two conventional CSRs. The three tested CSRs were similar in dimensions, cooling system, and amount of stored dates. The time required for cooling 800 kg of dates in the controlled CSR from 25 °C to the target temperature of 5 °C was approximately 48 h. The DUH precisely controlled the RH at the maximum RH set point of 80% in the tested CSR at 5 °C. The controlled RH at 80% has a positive impact on the physicochemical characteristics of the stored dates. It significantly reduced the weight loss of the fruits and preserved fruit mass, moisture content, water activity, firmness, and color parameters. However, no significant effect was observed on fruit dimensions, sphericity, and aspect ratio. The microbial loads of mesophilic aerobic bacteria, molds, and yeasts fell within the acceptable limits in all tested CSRs. Both stored date fruits and artificially infested dates showed no signs of insect activity in the controlled CSR at the temperature of 5 °C and RH of 80%. The DUH proved to be a promising technology for postharvest quality management for dates during cold storage.

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

scholarly journals Agricultural Technical Efficiency of Smallholder Farmers in Ethiopia: A Stochastic Frontier Approach

Land ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 246
Author(s):  
Markose Chekol Zewdie ◽  
Michele Moretti ◽  
Daregot Berihun Tenessa ◽  
Zemen Ayalew Ayele ◽  
Jan Nyssen ◽  
...  
Keyword(s):  
Technical Efficiency ◽  
Crop Production ◽  
Large Scale ◽  
Positive Impact ◽  
Smallholder Farmers ◽  
Stochastic Frontier ◽  
Crop Productivity ◽  
Small Scale ◽  
Stochastic Frontier Approach ◽  
Small Scale Irrigation

In the past decade, to improve crop production and productivity, Ethiopia has embarked on an ambitious irrigation farming expansion program and has introduced new large- and small-scale irrigation initiatives. However, in Ethiopia, poverty remains a challenge, and crop productivity per unit area of land is very low. Literature on the technical efficiency (TE) of large-scale and small-scale irrigation user farmers as compared to the non-user farmers in Ethiopia is also limited. Investigating smallholder farmers’ TE level and its principal determinants is very important to increase crop production and productivity and to improve smallholder farmers’ livelihood and food security. Using 1026 household-level cross-section data, this study adopts a technology flexible stochastic frontier approach to examine agricultural TE of large-scale irrigation users, small-scale irrigation users and non-user farmers in Ethiopia. The results indicate that, due to poor extension services and old-style agronomic practices, the mean TE of farmers is very low (44.33%), implying that there is a wider room for increasing crop production in the study areas through increasing the TE of smallholder farmers without additional investment in novel agricultural technologies. Results also show that large-scale irrigation user farmers (21.05%) are less technically efficient than small-scale irrigation user farmers (60.29%). However, improving irrigation infrastructure shifts the frontier up and has a positive impact on smallholder farmers’ output.

FAKTOR PENANGULANGAN TERJADINYA WASTE PADA PROYEK KONSTRUKSI DI SURABAYA

EXTRAPOLASI ◽  
2020 ◽  
Vol 17 (2) ◽  
pp. 1-10
Author(s):  
Michella Beatrix ◽  
Nurul Rochmah ◽  
Gede Sarya ◽  
Pebru Dwijayanto
Keyword(s):  
Waste Management ◽  
Construction Projects ◽  
Positive Impact ◽  
Scale Construction ◽  
Small Scale ◽  
Construction Costs ◽  
Warehouse Storage ◽  
The Right ◽  
The Impact ◽  
Material Requirements

AbstractLarge and small scale construction projects have waste that cannot be predicted in advance, even the amount cannot be predicted directly, whether it is in large or small amounts. The existence of waste can have a significant impact that can affect construction costs. Waste can have both negative and positive impacts. Good waste management will have a positive impact on the company in terms of cost, even time, and quality, but if the waste that occurs cannot be handled or managed properly it will harm the company in terms of cost, time, and even in terms of quality. In this case, the party that always gets the impact of the waste is the contractor.This study focuses on mitigating the occurrence of waste that is how to minimize it. Thisstudy uses the distribution of questionnaires to the contractor in Surabaya. The results of this study are 5 item indicators on how to minimize the highest ranking. The 5 items are Updating material requirements, Mixing, transporting, and placing concrete at the right time, Increasing the competence and expertise of labor, Provision of good and adequate material/warehouse storage facilities, and accurate material measurement.  Abstrak Proyek konstruksi dalam skala besar maupun kecil, memiliki waste yang tidak dapat diprediksi sebelumnya, bahkan jumlahnya pun tidak dapat diprediksi secara langsung, apakah itu dalam jumlah besar ataupun jumlah yang kecil. Adanya waste dapat memberikan dampak yang signifikan yang dapat mempengaruhi biaya konstruksi. Pada dasarnya waste dapat memberikan dampak negatif maupun positif. Pengelolaan waste yang baik akan memberikan dampak positif bagi perusahaan dalam segi biaya, bahkan waktu dan mutu, namun apabila waste yang terjadi tidak dapat diatasi atau dikelola dengan baik maka akan memberikan dampak negatif bagi perusahaan dalam segi biaya, waktu bahkan dalam hal mutu. Dalam hal ini pihak yang selalu mendapatkan dampak dari adanya waste adalah pihak kontraktorPenelitian ini memfokuskan pada faktor penanggulangan terjadinya waste yaitu cara meminimalisirnya. Penelitian ini menggunakan penyebaran kuisioner kepada pihak kontraktor di Surabaya. Hasil dari penelitian ini adalah 5 item indicator cara meminimalisir yang memiliki ranking tertinggi. 5 item tersebut adalah Updating kebutuhan material, Mencampur, mengangkut dan menempatkan beton pada waktu yang tepat, Meningkatkan kompetensi dan keahlian tenaga kerja, Penyediaan fasilitas penyimpanan material/gudang yang baik dan memadai, dan Pengukuran bahan yang akurat.

Lipids as early and minimally invasive biomarkers for Alzheimer disease

2021 ◽  
Vol 19 ◽  
Author(s):  
Esther Casas-Fernández ◽  
Carmen Peña-Bautista ◽  
Miguel Baquero ◽  
Consuelo Cháfer-Pericás
Keyword(s):  
Fatty Acids ◽  
Minimally Invasive ◽  
Neurodegenerative Disorder ◽  
Late Onset ◽  
Blood Lipid ◽  
Positron Emission ◽  
High Lipid ◽  
Lipid Metabolites ◽  
Invasive Techniques

: Alzheimer's disease (AD) is the most common neurodegenerative disorder worldwide. Specifically, typical late-onset AD is a sporadic form with a complex etiology that affects over 90% of patients. The current gold standard for AD diagnosis is based on the determination of amyloid status by the analysis of cerebrospinal fluid samples or by brain positron emission tomography. These procedures have some disadvantages to become widely used (expensive, invasive). As alternative, blood metabolites have recently emerged as promising AD biomarkers. Small molecules that cross the compromised AD blood-brain barrier, could be determined in plasma to improve clinical AD diagnosis at early stages through minimally invasive techniques. Specifically, lipids could play an important role in AD since brain has a high lipid content and they are present ubiquitously inside amyloid plaques. Therefore, a systematic review was performed with the aim of identifying blood lipid metabolites as potential early AD biomarkers. In conclusion, some lipid families (fatty acids, glycerolipids, glycerophospholipids, sphingolipids, lipid peroxidation compounds) showed impaired levels at early AD stages. Ceramide levels were significantly higher in AD subjects and polyunsaturated fatty acids levels were significantly lower in AD. Also, high arachidonic acid levels were found in AD patients in contrast to low sphingomyelin levels. Consequently, these lipid biomarkers could be used for minimally invasive and early AD clinical diagnosis.

ENVIRONMENTAL REGULATION IMPROVES THE FIRM PERFORMANCE IN THE PAPER INDUSTRY IN CHINA

2021 ◽  
pp. 1-32
Author(s):  
YONGLIANG YANG ◽  
LILI DING ◽  
YI LI
Keyword(s):  
Firm Performance ◽  
Positive Impact ◽  
Paper Industry ◽  
Average Treatment Effect ◽  
Small Scale ◽  
Positive Role ◽  
Firm Level ◽  
Innovation Ability ◽  
The Relationship

This research develops a difference-in-differences (DID) model to explore the relationship between environmental policy (The Measures for the Administration of Permits for the Discharge of Key Water Pollutants in the Huaihe and Taihu River Basins, MAPD) and the performance of firms involved in the paper and paper products industry (MPP) in China. Cost and innovation are introduced as mediators to explore the mediating effects. A firm-level dataset from 1998 to 2007 is adopted for empirical study. The findings support the positive role of the MAPD, and the average treatment effect is 0.016.The heterogeneity analysis shows that the MAPD exerts a positive impact on non-state-owned and small-scale enterprises, with coefficients of 0.018 and 0.021, respectively. Moreover, MAPD increases enterprise costs harming firm performance. On the other hand, it can promote firm performance by improving innovation ability.

‘We are still here’: The impacts of street music and street art during the 2020 London lockdowns

2021 ◽  
Vol 00 (00) ◽  
pp. 1-12
Author(s):  
Jonathan Barnes
Keyword(s):  
Positive Impact ◽  
Street Art ◽  
Small Scale ◽  
School And Community ◽  
Children’S Voices ◽  
Local Cohesion

This article summarises the impacts of a spontaneous arts initiative involving the residents of eight London streets during the 2020 lockdowns. A local arts organization devised small-scale, informal street music projects that were evaluated by the residents themselves. Responses suggested that such events had a strong positive impact on the feelings of community. Common responses included reaffirming the importance of local cohesion, recognizing music as an accessible means of developing new connections in ‘distanced’ conditions and a new appreciation of family togetherness. Those involved suggested that researchers could learn much about the characteristics of cohesive, supportive communities from similar initiatives. The project confirmed that more research was needed on the role schools could play in bringing communities together and how music can be used to build bridges between school and community. Feedback raised questions about the absence of children’s voices in post-COVID-19 planning for ‘the recovery curriculum’.

scholarly journals Yeast-Mediated Stereoselective Reduction of α-Acetylbutyrolactone

2018 ◽  
Vol 8 (8) ◽  
pp. 1334 ◽  
Author(s):  
Wanda Mączka ◽  
Katarzyna Wińska ◽  
Małgorzata Grabarczyk ◽  
Barbara Żarowska
Keyword(s):  
Organic Solvent ◽  
Yarrowia Lipolytica ◽  
Enantiomeric Excess ◽  
Deep Eutectic Solvent ◽  
Ester Group ◽  
Resting Cells ◽  
Media Composition ◽  
Yeast Strains ◽  
Optically Pure ◽  
Diastereomeric Excess

α’-1’-Hydroxyethyl-γ-butyrolactone—a product of reduction of α-acetylbutyrolactone possesses two stereogenic centres and two reactive functionalities (an alcohol and an ester group). Additionally, this compound has a similar structure to γ-butyrolactone (GBL) which is psychoactive. In the present work, biotransformation using seven yeast strains was used to obtain anti stereoisomers of α’-1’-hydroxyethyl-γ-butyrolactone. The process was carried out in both growing and resting culture. The effect of media composition and organic solvent addition on stereoselectivity and effectiveness of biotransformation was also studied. After one day of transformation, optically pure (3R,1’R)-hydroxylactone was obtained by means of Yarrowia lipolytica P26A in YPG medium (yeast extract (1%), peptone (2%) and glucose (2%)). In turn, the use of resting cells culture of Candida viswanathi AM120 in the presence of 10% DES (deep eutectic solvent) allowed us to obtain a (3S,1’S)-enantiomer with de = 85% (diastereomeric excess) and ee 76% (enantiomeric excess).

Sign in / Sign up

Export Citation Format

Share Document