scholarly journals Oak (Quercus robur) Associated Endophytic Paenibacillus sp. Promotes Poplar (Populus spp.) Root Growth In Vitro

2021 ◽  
Vol 9 (6) ◽  
pp. 1151
Author(s):  
Dorotėja Vaitiekūnaitė ◽  
Sigutė Kuusienė ◽  
Emilija Beniušytė
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Quercus Robur ◽  
Chemical Fertilizers ◽  
Iaa Production ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Populus Spp

Soil fertilization is necessary for high-demand crop production in agriculture and forestry. Our current dependence on chemical fertilizers has significant harmful side effects. Biofertilization using microorganisms is a sustainable way to limit the need for chemical fertilizers in various enterprises. Most plant endophytic bacteria have thus far been unstudied for their plant growth promoting potential and hence present a novel niche for new biofertilizer strains. We isolated English oak (Quercus robur) endophytic bacteria and tested them for plant growth promoting traits (PGPTs) such as nitrogen fixation, phosphate mineralization/solubilization, siderophore and indole-3-acetic acid (IAA) production. We also investigated the effect the selected isolate had on poplar (Populus spp.) microshoot vegetative growth parameters in vitro. In total 48 bacterial strains were isolated, attributed to Bacillus, Delftia, Paenibacillus, Pantoea and Pseudomonas genera. All the isolates displayed at least three PGPTs, with 39.6% of the isolates displaying all five (all were Pseudomonas spp.) and 18.75% displaying four. Based on relative abundance, Paenibacillus sp. isolate was selected for the poplar microshoot inoculation study. The isolate had a significant positive effect on poplar microshoot root growth and development. Two tested poplar genotypes both had increased lateral root number and density, fresh and dry root biomass. Furthermore, one genotype had increased length and number of adventitious roots as well as a decrease in fresh aboveground biomass. The root enhancement was attributed to IAA production. We propose this isolate for further studies as a potential biofertilizer.

scholarly journals Plant growth‐promoting activity of endophytic bacteria from sweet sorghum (Sorghum bicolor (L.) Moench)

2021 ◽  
Vol 26 (4) ◽  
pp. 190
Author(s):  
Charlie Ester De Fretes ◽  
Donny Widianto ◽  
Yekti Asih Purwestri ◽  
Tri Rini Nuringtyas
Keyword(s):  
Plant Growth ◽  
Sweet Sorghum ◽  
Endophytic Bacteria ◽  
Environmental Damage ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Chemical Fertilizers ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Application of high levels of chemical fertilizers for optimal growth of sweet sorghum causes environmental degradation. Plant growth‐promoting bacteria have biotechnological importance because they can improve the growth and health of important agronomic plants. This study aimed to isolate, characterize, and identify endophytic bacteria associated with sweet sorghum (cv. KCS105), and also to study the inoculation effects of selected isolates on sorghum growth. In this study, 35 isolates were evaluated for their ability to support plant growth. The results showed that seven isolates were diazotrophic, six were capable of dissolving phosphate, six produced IAA and could detect ACC‐deaminase activity, and three inhibited the growth of pathogenic fungi. Nine isolates exhibiting mechanisms for promoting plant growth from the Alphaproteobacteria (Devosia), Firmicutes (Bacillus, Paenibacillus, Staphylococcus), and Actinobacteria (Microbacterium, Brachybacterium) phyla were identified. In addition, the Paenibacillus sp. BB7, Bacillus sp. PIB1B, and Bacillus sp. PLB1B isolates showed increasing effects on plant growth in greenhouse tests. Endophytic bacterial isolates which display plant growth‐promoting features can potentially be employed as biofertilizer agents. They may also address environmental damage problems resulting from the use of chemical fertilizers and pesticides.

scholarly journals A Metabolic Signature of the Beneficial Interaction of the Endophyte Paenibacillus sp. Isolate and In Vitro–Grown Poplar Plants Revealed by Metabolomics

2009 ◽  
Vol 22 (8) ◽  
pp. 1032-1037 ◽  
Author(s):  
Christian Scherling ◽  
Kristina Ulrich ◽  
Dietrich Ewald ◽  
Wolfram Weckwerth
Keyword(s):  
Endophytic Bacteria ◽  
Tricarboxylic Acid Cycle ◽  
Primary Metabolism ◽  
Plant Metabolites ◽  
Metabolic Signature ◽  
Mutualistic Interaction ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Metabolic profiling via gas chromatography coupled to mass spectrometry was used to investigate the influence of endophytic bacteria on shoots of in vitro-grown poplar plants free from culturable endophytic bacteria. The results demonstrate that the occurrence of an endophytic Paenibacillus strain strongly affects the composition of the plant metabolites of in vitro-grown poplars. Eleven metabolites were significantly changed between inoculated and non-inoculated poplar plants as determined by two independent experiments. Detected shifts in the primary metabolism of the poplar plants pointed to a mutualistic interaction between bacteria able to fix nitrogen and the host plant with altered nitrogen assimilation patterns. The corresponding metabolic signature comprises increased asparagine and urea levels as well as depleted sugars and organic acids of the tricarboxylic acid cycle. These observations coincide with the fact that the Paenibacillus sp. strain P22 is able to grow without nitrogen in the medium, indicating nitrogen fixation from the air also known from other Paenibacillus spp. In combination with the detected plant-growth-promoting effects of the endophyte Paenibacillus P22, a novel mutualistic interaction is observed.

scholarly journals Plant growth-promoting activity in bean plants of endophytic bacteria isolated from Echeveria laui

2021 ◽  
Vol 5 (2) ◽  
pp. 65
Author(s):  
Anderson Emmer ◽  
João Arthur Dos Santos Oliveira ◽  
Andressa Domingos Polli ◽  
Julio Cesar Polonio ◽  
Leonardo Hamamura Alves ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Arid Environment ◽  
Bacterial Isolates ◽  
Phaseolus Vulgaris L ◽  
Bean Plants ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

Echeveria laui (Crassulaceae) is commonly commercialized due to its drought-tolerance capacity and to its rosette-shaped aesthetics. Since endophytes associated with plants from a dry or arid environment have scarcely been analyzed as yet, current research comprises the isolation of leaf endophytic bacteria from E. laui (one five-year-old and one two-year-old plants) investigating plant growth-promoting endophytic bacteria which may solubilize phosphate, fix nitrogen, produce exopolysaccharides/IAA and antagonize phytopathogens. Isolation by the maceration methodology provided a colonization rate of 1.98 x109 CFU g-1 for the two-year-old plant and 1.14 x 1010 CFU g-1 for the five-year-old one. All 40 isolates evaluated showed in vitro plant growth-promoting agent’s abilities, with emphasis on EG04, ELG18, and ELP06.  The capacity of the three best bacterial isolates were evaluated under greenhouse conditions in common and black bean (Phaseolus vulgaris L.) plants. Based on the sequencing of the 16S rRNA region and phylogenetic analysis, the three endophytes were identified as Pantoea sp. (ELG04 and ELG18) and Erwinia sp. (ELP06). Under greenhouse conditions, statistically significant differences were found among the plants treated with the three endophytes when compared to control plants for fresh and dry shoot, root biomass and length.

Plant growth promoting potential of multifarious endophytic Pseudomonas lini strain isolated from Cicer arietinum L.

2021 ◽  
pp. 1-11
Author(s):  
Rajat Maheshwari ◽  
Namita Bhutani ◽  
Pradeep Kumar ◽  
Pooja Suneja
Keyword(s):  
Plant Growth ◽  
Organic Acid ◽  
Cicer Arietinum ◽  
Endophytic Bacteria ◽  
Growth Parameters ◽  
Biotic Stresses ◽  
Iaa Production ◽  
Rdna Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Chickpea (Cicer arietinum), one of the major pulse crops in India, endured extreme reduction in production due to various abiotic and biotic stresses. Endophytic bacteria residing in the nodules and roots of chickpea plants enable host in combating these stresses. Twenty one endophytic bacteria isolated from nodules and roots of chickpea were screened for multiple plant growth promoting traits like ammonia, organic acid, siderophore, hydrogen cyanide (HCN) and phytohormone indole acetic acid (IAA) production. Out of these, 86% isolates produced ammonia, around 50% isolates produced organic acid, HCN and siderophore, 29% isolates produced ACC (1-aminocyclopropane-1-carboxylic acid) deaminase, while only 14% isolates solubilized phosphate. Interestingly, all the isolates were able to produce IAA ranging from 11.6–85.2 μg/ml, isolate CPJN 13 being the maximum IAA producer (85.5±2.33 μg/ml). Isolate CPJN13 was selected for IAA optimization studies. The yield of IAA increased up to 4 fold i.e. 331±4.96 μg/ml at optimized conditions. IAA production was also confirmed by TLC and HPLC analysis of crude IAA extract. The application of CPJN13 on chickpea seedlings resulted in significant increase in plant growth parameters. The 16S rDNA sequencing of CPJN13 revealed its similarity with Pseudomonas lini strain and submitted to NCBI with accession number MF574502. To best of our knowledge, this is the first report of the presence of P. lini as endophyte in chickpea nodules. The results of this study imply that the endophytic P. lini has a potential role to enhance the plant growth.

scholarly journals Isolation and characterization of plant growth promoting endophytic bacteria isolated from Vigna radiata

2018 ◽  
Author(s):  
Pooja Suneja ◽  
Rajat Maheshwari ◽  
Namita Bhutani
Keyword(s):  
Plant Growth ◽  
Vigna Radiata ◽  
Endophytic Bacteria ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Restriction Analysis ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

A total of 22 endophytic bacteria were isolated from roots and nodules of Vigna radiata (mungbean) obtained from Jind district, Haryana. These were characterized on the basis of plant growth promoting traits. Almost all the endophytic bacteria produced IAA with maximum production of 81.63µg/ml by isolate MJiR8. Among these, 100% root isolates and 84.6% nodule isolates resulted in in vitro root growth promotion of mungbean seedlings. All the isolates produced ammonia; eighteen (all root and nine nodule isolates) produced organic acid while only four root isolates were positive for siderophore production. The four isolates produced hydrogen cyanide and out of these only MJiR9 inhibited the growth of fungal pathogens Fusarium oxysporium and Aspergillus niger. All the endophytes were used to determine molecular diversity by ARDRA (Amplified Ribosomal DNA Restriction Analysis) Results revealed that the nodule isolates were more diverse, being present in separate clusters, in comparison to root isolates which were grouped together in cluster III.

scholarly journals Potency of Endophytic Bacteria from Brotowali (Tinospora crispa) as Biocontrol of Sclerotium rolfsii and Plant Growth Promoting

2021 ◽  
Vol 16 (3) ◽  
pp. 95-104
Author(s):  
Abdul Munif ◽  
Kholil Ma’ruf
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Sclerotium Rolfsii ◽  
Plant Growth Promoting ◽  
Growth Promoting

Sclerotium rolfsii merupakan salah satu patogen penting pada kacang tanah yang dapat menyebabkan penyakit busuk pangkal batang. S. rolfsii dapat bertahan hidup di dalam tanah dan membentuk struktur dorman. Penelitian ini bertujuan mengevaluasi bakteri endofit dari tanaman brotowali yang berpotensi sebagai agens hayati yang efektif untuk mengendalikan S. rolfsii dan mengetahui pengaruhnya pada pertumbuhan tanaman kacang tanah. Bakteri endofit diisolasi dari akar dan batang tanaman brotowali dengan metode sterilisasi permukaan. Bakteri endofit yang berhasil diisolasi dari tanaman brotowali ialah 415 isolat dan 153 isolat lolos uji keamanan hayati. Sebanyak 7 isolat bakteri endofit, yaitu BBT25, BBT90, BBT102, BBT106, BBT110, BBT130, dan BSK18 berpotensi menekan S. rolfsii. Isolat BBT106 mampu menekan pertumbuhan S. rolfsii sebesar 73% secara in vitro. Isolat BBT110 dan BSK18 mampu menekan kejadian penyakit busuk pangkal batang sebesar 58% dan 67% pada penelitian di rumah kaca. Ketujuh isolat bakteri endofit brotowali mampu meningkatkan pertumbuhan tanaman kacang tanah pada percobaan di rumah kaca.

scholarly journals Isolation, Identification and Evaluation of Indigenous Plant Growth Promoting Bacterium Klebsiella pneumoniae PNE1

2021 ◽  
Vol 8 (6) ◽  
pp. 47-56
Author(s):  
Pooja Gupta ◽  
Minal Trivedi ◽  
Harsha Soni
Keyword(s):  
Klebsiella Pneumoniae ◽  
Plant Growth ◽  
Klebsiella Pneumonia ◽  
Plant Growth Promoting Bacteria ◽  
Chemical Fertilizers ◽  
Pgp Traits ◽  
Iaa Production ◽  
Antibiotic Susceptibility Test ◽  
Plant Growth Promoting ◽  
Growth Promoting

Application of chemical fertilizer is an integral practice to optimize crop productivity, but the dominant use of chemical fertilizers contributes largely to the deterioration of the environment, leads to loss of soil fertility, increases pollution, and causes hazardous diseases. Hence, the chemical fertilizers, pesticides and other supplements are being replaced by the plant growth promoting bacteria (PGPB) due to their improved potency and environment friendly nature. Plant growth-promoting bacteria (PGPB) can enhance plant growth by a wide variety of mechanisms like Phosphate (P) solubilization, Potassium solubilisation, siderophore production, biological nitrogen fixation and Indole acetic acid (IAA) production. The Klebsiella species is also known to exhibit important PGP traits like solubilization of phosphate, phytohormone production and good germination potential. In present study the Klebsiella pneumoniae PNE1 was selected from the isolates obtained from vegetable waste collected from Kadi market. The isolate was selected on the basis of its ability for Nitogen fixation, Phosphate solubilization, Potassium solubilization, IAA production, EPS production and biopolymer degradation. The molecular identification through 16S rRNA gene sequence, confirmed the isolate as Klebsiella pneumonia PNE1. Quantitative analysis of ammonia production revealed that isolate Klebsiella pneumonia PNE1 produced 0.5 µg/ml of ammonia (NH3) on 6th day of incubation and produced 0.09 µg/ml Nitrite after 8th day of incubation. The Phosphate solubilisation Index (SI) of the isolate was 4.16 and the isolate released 177.50 μg/ml Phosphate. The qualitative estimation of Potassium solubilisation by the isolate Klebsiella pneumoniae PNE1 in terms of Potassium solubilisation zone was found to increase gradually from day 1 to 7 days and was maximum at 2nd day with a KSI of 3.6. The isolate Klebsiella pneumoniae PNE1 released 29.94 mg/l Potassium on 21th day of incubation respectively. The IAA production was found to be 94.96 µg/ml. The maximum the EPS yield was 11.3 mg/ml. The Klebsiella pneumonia PNE1 had capacity to degrade Cellulose, Pectin and Xylan i.e. all biopolymers tested. The antibiotic susceptibility test indicated that isolate was sensitive to all 22 antibiotics tested. The Klebsiella pneumonia PNE1 thus, shows important plant growth promoting traits and can be used in a bio-fertilizer formulation for sustainable agriculture.

Screening of cultivable endophytic bacterial isolates for their plant growth promoting activity in rice

2017 ◽  
Vol 51 (05) ◽  
Author(s):  
Jannathul Firdous ◽  
Bhore Subhash J.
Keyword(s):  
Plant Growth ◽  
Root Length ◽  
Endophytic Bacteria ◽  
Oryza Sativa L ◽  
Positive Root ◽  
Bacterial Isolates ◽  
Rice Seedlings ◽  
Chemical Fertilizers ◽  
Plant Growth Promoting ◽  
Growth Promoting

Large amounts of chemical fertilizers are being used to enhance the growth and development of the rice (Oryza sativa L.) crop. Chemical fertilizers are too expensive; hence, endophytic bacteria with growth promoting activity can be considered to promote the crop plant growth to minimize the usage of chemical fertilizers and to have sustainable rice cultivation. This study was undertaken to explore the potential of endophytic bacterial isolates (EBIs) which were isolated previously. The objective of this study was to evaluate 800 EBIs for auxin-like activity and its effect on early seedlings growth of rice. Surface sterilized rice seeds were treated separately with different EBIs and allowed to grow in Petriplates (containing sterile cotton) for 10 days. On the 10th day, the root length of the rice seedlings was measured and recorded to determine the auxin-like activity of EBIs. Results showed that 9 different isolates out of 800 EBIs screened to have significant effect on rice root and shoot growth when compared to control. The increase in root length was in the range of 58.30% to 81.68%, in comparison to root length of seedlings from control category. Based on the results, it was concluded that the positive root growth promoting response of rice seedlings could be due to the production of auxin-like compounds by the EBIs. These research findings could be useful in developing endophytic bacteria based formulation to enhance the growth of rice crop.

scholarly journals Survey of plant growth promoting and antagonistic traits in winter wheat grain endophytic bacteria

2021 ◽  
pp. 66-72
Author(s):  
Alina Pastoshchuk ◽  
Yuliia Yumyna ◽  
Pavlyna Zelena ◽  
Larysa Skivka
Keyword(s):  
Plant Growth ◽  
Winter Wheat ◽  
Endophytic Bacteria ◽  
Wheat Variety ◽  
Wheat Grain ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

The aim of this work was to isolate endophytic bacteria from wheat grains and to evaluate their plant growth promoting traits (PGPT) as well as an inhibitory effect on P. syringae pv. atrofaciens (McCulloch) growth. Endophytic bacteria were isolated by a culture-dependent protocol from the grains of winter wheat variety of Ukrainian selection Podolyanka with high resistance to syringae. Totally 2.7±0.09 CFU/1 g of dry wheat grain were isolated, ten cultivable bacterial isolates were obtained. Spore-forming bacilli predominated in the wheat grain endophytic community. Gram-negative fermenting and non-fermenting rod-shaped bacteria and Gram-positive cocci were also present. Seven out of ten isolates possessed numerous plant growth promoting traits including phosphate solubilization, oligonitrotrophy, and indolic compound producing. Two isolates possessed antagoniscic activity against syringae in vitro along with plant growth promoting features. According to biochemical profiling and mass-spectrophotometric identification, these two isolates were assigned to Paenibacillus and Brevibacillus genera. These endophytic bacteria can be considered as promising objects for agrobiotechnology. However, more research is needed to confirm their biotechnological potential in planta experiments

Sign in / Sign up

Export Citation Format

Share Document