scholarly journals Neospora caninum and/or Toxoplasma gondii Seroprevalence: Vaccination against PCV2 and Muscle Enzyme Activity in Seropositive and Seronegative Pigs

2021 ◽  
Vol 9 (5) ◽  
pp. 1097
Author(s):  
Labrini V. Athanasiou ◽  
Vasileios G. Papatsiros ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Anna Dedousi
Keyword(s):  
Enzyme Activity ◽  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Human Infection ◽  
Porcine Circovirus ◽  
Muscle Enzyme ◽  
Blood Samples ◽  
Zoonotic Pathogen ◽  
Muscle Enzyme Activity ◽  
Immunofluorescence Antibody

Neospora caninum and Toxoplasma gondii affect both humans and animals worldwide. To investigate their seroprevalence and differences in seropositivity between pigs vaccinated and unvaccinated against porcine circovirus 2 (PCV2), as well as differences in muscle enzyme activity between seropositive and seronegative pigs, blood samples were collected from 380 sows. Antibodies against T. gondii and N. caninum were detected by an indirect immunofluorescence antibody (IFA) assay, while the activities of creatine kinase (CK) and aspartate aminotransferase (AST) were biochemically assessed. Out of the 364 sows finally included in the study, 4.4%, 3.5%, and 0.5% were seropositive to T. gondii, N. caninum, or both. A significantly higher percentage of seropositivity against T. gondii and/or N. caninum in PCV2 unvaccinated pigs compared with vaccinated pigs was observed. Increased serum activities of CK and AST were detected in 71.43% and 100% of only against T. gondii (T+) and 63.64% and 90.91% of only against N. caninum (N+) seropositive sows, respectively, and were significantly higher compared to seronegative animals. T. gondii and N. caninum seropositivity, especially in presumed immunocompromised pigs, and the evidence of muscle damage highlight their importance as a zoonotic pathogen and animal model of human infection, respectively.

scholarly journals Occurrences of anti-Toxoplasma gondiiand anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Vivien Midori Morikawa ◽  
Cristina Kraemer Zimpel ◽  
Igor Adolfo Dexheimer Paploski ◽  
Maria do Carmo Custódio de Souza Hunold Lara ◽  
Eliana Monteforte Cassaro Villalobos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Southern Brazil ◽  
Intermediate Hosts ◽  
Blood Samples ◽  
Ammotragus Lervia ◽  
Barbary Sheep ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Barbary sheep (Ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. Blood samples from 17 Barbary sheep at the Curitiba zoo were collected to evaluate occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (IFAT). Anti-T. gondii and anti-N. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. The present study has shown that Barbary sheep at Curitiba zoo were exposed to T. gondii andN. caninum and therefore may act as intermediate hosts, spreading toxoplasmosis and neosporosis within and between species in shared areas.

scholarly journals Survey of Toxoplasma gondii, Neospora caninum and Sarcocystis neurona antibodies in wild red-tailed Amazon parrots (Amazona brasiliensis)

2020 ◽  
Vol 29 (1) ◽  
Author(s):  
Ana Paula Sato ◽  
Frederico Fontanelli Vaz ◽  
Aline Luiza Konell ◽  
Marilia de Oliveira Koch ◽  
Rafaela Furioso Ferreira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Plasma Samples ◽  
Sarcocystis Neurona ◽  
Blood Samples ◽  
Obligate Intracellular ◽  
Intracellular Parasites ◽  
Antibody Tests ◽  
Immunofluorescence Antibody ◽  
Near Threatened

Abstract Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are obligate intracellular parasites within the phylum Apicomplexa. The red-tailed Amazon parrot (Amazona brasiliensis) is a near-threatened species of psittacine that is endemic to the Atlantic Forest of Brazil and has been designated as a bioindicator because of its sensitivity to environmental qualitative status and changes. The aim of this study was to evaluate the presence of antibodies against T. gondii, N. caninum and S. neurona in wild red-tailed Amazon parrot nestlings on Rasa Island, Brazil. Blood samples were collected from 51 parrots and plasma samples were stored at – 20 °C until immunofluorescence antibody tests (IFAT) were performed. Antigen slides were prepared using tachyzoites of T. gondii (RH strain) and, N. caninum (NC-1 strain) and using merozoites of S. neurona (SNR37 strain). Plasma samples were tested at initial dilutions of 1:16 for T. gondii, 1:50 for N. caninum and 1:5 for S. neurona. An anti-chicken antibody conjugated with FITC was used as a secondary antibody at 1:50 dilution. No antibodies for any of these three protozoa were found, thus suggesting that these wild red-tailed Amazon parrot nestlings had not been exposed to these parasites.

scholarly journals Seroprevalence of Toxoplasma gondii in dogs of riverside communities of Mato Grosso Pantanal, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 531-535 ◽  
Author(s):  
Juliana Yuki Rodrigues ◽  
Arleana do Bom Parto Ferreira de Almeida ◽  
Eveline da Cruz Boa Sorte ◽  
Naiani Domingos Gasparetto ◽  
Felipe Augusto Constantino Seabra da Cruz ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Antibody Test ◽  
Human Infection ◽  
Mato Grosso ◽  
Exact Test ◽  
Worldwide Distribution ◽  
Significant Difference ◽  
Chi Squared ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract Toxoplasma gondii is an intracellular protozoan with worldwide distribution and dogs act as sentinels of human infection. This search aimed to determine the occurrence of antibodies against T. gondii in dogs of the communities on the Cuiabá River, Mato Grosso and variables associated with infection. The dogs of the riverside communities in Cuiabá River, which includes Barranco Alto, Praia do Poço, Engenho Velho, Varginha, Bom Sucesso, Passagem da Conceição and São Gonçalo Beira Rio, were evaluated for the presence of T. gondii antibodies by indirect immunofluorescence antibody test (IFAT). The prevalence and factors associated with infection were calculated by chi-squared test (χ2) or Fisher’s exact test, and univariate and multiple analysis. Of the 248 dogs surveyed, 107 (43.1%) were seropositive for T. gondii. The seroprevalence ranged from 25.6% to 64.3%. There was no statistically significant difference between the communities studied (p > 0.05). As for the associated factors, the only statistically significant factor was that of dogs living with cats (p = 0.02), with approximately twice the risk of acquiring infection. In conclusion, the seroprevalence in dogs of riverside communities in the Baixada Cuiabana demonstrated that high rates of infection, being the factor associated with infection, contact with domestic cats.

Effects of iron deficiency on endurance and muscle enzyme activity in man

1986 ◽  
Vol 18 (2) ◽  
pp. 156???161 ◽  
Author(s):  
FREDRIK CELSING ◽  
EVA BLOMSTRAND ◽  
BJ??RN WERNER ◽  
PETER PIHLSTEDT ◽  
BJ??RN EKBLOM
Keyword(s):  
Enzyme Activity ◽  
Iron Deficiency ◽  
Muscle Enzyme ◽  
Muscle Enzyme Activity

scholarly journals Increased muscle enzyme activity after yoga breathing during an exacerbation of asthma.

Thorax ◽  
1988 ◽  
Vol 43 (9) ◽  
pp. 731-732 ◽  
Author(s):  
F M Tamarin ◽  
R Conetta ◽  
R D Brandstetter ◽  
H Chadow
Keyword(s):  
Enzyme Activity ◽  
Muscle Enzyme ◽  
Muscle Enzyme Activity

scholarly journals Toxoplasma gondii, Neospora caninum and Leishmania spp. serology and Leishmania spp. PCR in dogs from Pirassununga, SP

2015 ◽  
Vol 24 (4) ◽  
pp. 454-458 ◽  
Author(s):  
Nathália Mendonça de Seabra ◽  
Vanessa Figueredo Pereira ◽  
Marcos Vinícius Kuwassaki ◽  
Julia Cristina Benassi ◽  
Trícia Maria Ferreira de Sousa Oliveira
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Seropositivity Rate ◽  
Three Samples ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
The City ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract We examined the presence of antibodies against the parasites Toxoplasma gondii, Neospora caninum, and Leishmania spp., as well the presence of DNA from Leishmania spp., in dogs from Pirassununga - SP. The seropositivity rate was compared with the animals’ originating location. Three hundred seventy-three blood samples from the county’s kennel and local veterinary clinics were collected and analyzed. A total of 300 samples were tested for T. gondii and N. caninum using an indirect immunofluorescence antibody test (IFAT); 45% (135/300) were positive for T. gondii and 24.3% (73/300) were positive for N. caninum. Three hundred seventy-three samples were tested for Leishmania spp. using the IFAT. Of these, 4.6% (17/373) were positive. Additionally, 145 samples were tested using a polymerase chain reaction (PCR); of these samples, 0.7% (1/145) was positive. Considering the results, we conclude that these parasites are present in the city of Pirassununga - SP and that the animals have contact with the protozoan. It is therefore necessary to create methods for disease prevention to maintain both animal and human health in regard to leishmaniasis and toxoplasmosis.

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