scholarly journals Experimental Infection of the Biomphalaria glabrata Vector Snail by Schistosoma mansoni Parasites Drives Snail Microbiota Dysbiosis

2021 ◽  
Vol 9 (5) ◽  
pp. 1084
Author(s):  
Anaïs Portet ◽  
Eve Toulza ◽  
Ana Lokmer ◽  
Camille Huot ◽  
David Duval ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Control Strategies ◽  
Amplicon Sequencing ◽  
Biomphalaria Glabrata ◽  
Microbiota Composition ◽  
Microbial Composition ◽  
Bacterial Microbiota ◽  
Individual Scale ◽  
The Individual ◽  
Host Parasite

Host-parasite interaction can result in a strong alteration of the host-associated microbiota. This dysbiosis can affect the fitness of the host; can modify pathogen interaction and the outcome of diseases. Biomphalaria glabrata is the snail intermediate host of the trematode Schistosoma mansoni, the agent of human schistosomiasis, causing hundreds of thousands of deaths every year. Here, we present the first study of the snail bacterial microbiota in response to Schistosoma infection. We examined the interplay between B. glabrata, S. mansoni and host microbiota. Snails were infected and the microbiota composition was analysed by 16S rDNA amplicon sequencing approach. We demonstrated that the microbial composition of water did not affect the microbiota composition. Then, we characterised the Biomphalaria bacterial microbiota at the individual scale in both naive and infected snails. Sympatric and allopatric strains of parasites were used for infections and re-infections to analyse the modification or dysbiosis of snail microbiota in different host-parasite co-evolutionary contexts. Concomitantly, using RNAseq, we investigated the link between bacterial microbiota dysbiosis and snail anti-microbial peptide immune response. This work paves the way for a better understanding of snail/schistosome interaction and should have critical consequences in terms of snail control strategies for fighting schistosomiasis disease in the field.

scholarly journals Infection of theBiomphalaria glabrata vector snailbySchistosoma mansoniparasites drives snail microbiota dysbiosis

2018 ◽  
Author(s):  
Anaïs Portet ◽  
Eve Toulza ◽  
Ana Lokmer ◽  
Camille Huot ◽  
David Duval ◽  
...  
Keyword(s):  
Control Strategies ◽  
Amplicon Sequencing ◽  
Biomphalaria Glabrata ◽  
Individual Level ◽  
Host Parasite Interactions ◽  
Bacterial Microbiota ◽  
Rnaseq Data ◽  
16S Rdna Amplicon Sequencing ◽  
The Individual ◽  
Host Parasite

SummaryHost-associated microbiota cari affect the fitness of its host i η a number of ways, including the modification of host-parasite interactions and thus the outcome of disease.Biomphalaria glabratais the vector snail of the trematodeSchistosoma mansoni,the agent of human schistosomiasis, causing hundreds of thousands of deaths every year. Here, we present the first study of the snail bacterial microbiota in response toSchistosomainfection. To examine the interplay betweenB. glabrata, S. mansoniand snail microbiota, snails were infected and the microbiota composition was analysed by massive 16S rDNA amplicon sequencing approach. We characterized theBiomphalariabacterial microbiota at the individual level in both naive and infected snails. Sympatric and allopatric strains of parasites were used for infections and re­infections to analyse the modification or dysbiosis of snail microbiota in different host-parasite co-evolutionary contexts. Concomitantly, using RNAseq data, we investigated the link between bacterial microbiota dysbiosis and snail anti-microbial peptide immune response. This work paves the way for a better understanding of snail/schistosome interaction, and would have critical consequences in terms of snail control strategies for fighting schistosomiasis disease in the field.

Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda)

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 653-664 ◽  
Author(s):  
C. J. Bayne ◽  
E. S. Loker ◽  
Mary A. Yui
Keyword(s):  
Schistosoma Mansoni ◽  
Plasma Proteins ◽  
Biomphalaria Glabrata ◽  
Rabbit Antibody ◽  
Tegumental Surface ◽  
Immunological Interactions ◽  
Resistant Strains ◽  
Host Parasite ◽  
Susceptibility And Resistance

SUMMARYThe tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plasma antigens are present on the surface at near steady state. Though a wide variety of peptides is acquired, there is selection. Furthermore, some differences occur in the peptides acquired from the plasma of susceptible and resistant strains of snail. Acquired antigens are rapidly processed, and are predominantly undetectable in tegumental extracts after a few hours. In contrast, rabbit antibody on sporocysts remains in situ for at least 48 h, so under some conditions there is stable expression of certain tegumental antigenic determinants.These data, obtained using antibodies to snail plasma antigens and to sporocyst tegumental antigens, are discussed in the light of current ideas on the cellular and molecular basis of susceptibility and resistance in this host#parasite system.

Transient non-susceptibility toSchistosoma mansoniassociated with atrial amoebocytic accumulations in the snail hostBiomphalaria glabrata

Parasitology ◽  
1987 ◽  
Vol 95 (3) ◽  
pp. 499-505 ◽  
Author(s):  
C. S. Richards ◽  
D. J. Minchella
Keyword(s):  
Population Dynamics ◽  
Schistosoma Mansoni ◽  
Genetic Control ◽  
Old Age ◽  
Biomphalaria Glabrata ◽  
Egg Laying ◽  
Snail Population ◽  
Host Parasite ◽  
Variable Susceptibility

SUMMARYIn someBiomphalaria glabrata–Schistosoma mansonicombinations snails are susceptible to infection as juveniles, but have variable susceptibility as adults. These snails become non-susceptible at the onset of egg-laying and typically revert to susceptibility in old age. Certain stocks ofB. glabratahave the capacity to form amoebocytic accumulations in the atrium, and this ability is under genetic control. The atrial amoebocytic accumulations are transitory, typically appearing at onset of egg-laying and disappearing after a few months. A snail stock which has genetic tendencies for both adult variable susceptibility and atrial amoebocytic accumulations was studied. An association between the time of occurrence of adult non-susceptibility and atrial accumulation is revealed as snails never became infected withS. mansoniwhen amoebocytic accumulations were present. Developing parasites, however, were not necessarily encapsulated and destroyed by amoebocytes. Some sporocysts were able to delay development until the amoebocytic accumulations disappeared. The timing of atrial amoebocytic accumulations and resulting transient non-susceptibility in this host-parasite combination could influence snail population dynamics.

scholarly journals Supplementation of a lacto-fermented rapeseed-seaweed blend promotes gut microbial- and gut immune-modulation in weaner piglets

2020 ◽  
Author(s):  
Yan Hui ◽  
Paulina Tamez-Hidalgo ◽  
Tomasz Cieplak ◽  
Gizaw Dabessa Satessa ◽  
Witold Kot ◽  
...  
Keyword(s):  
Basal Diet ◽  
Amplicon Sequencing ◽  
Saccharina Latissima ◽  
Blood Levels ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Colon Mucosa ◽  
Gut Health ◽  
Microbial Composition ◽  
Final Body Weight

AbstractThe direct use of medical zinc oxide (ZnO) in feed will be abandoned after 2022 in Europe, leaving an urgent need for substitutes to prevent post-weaning disorders. This study assessed whether rapeseed meal added two brown macroalagae species (Saccharina latissima and Ascophylum nodosum) and fermented using lactic acid bacteria (FRS) could improve piglet performance and gut health. The weaned piglets were fed one of three different feeding regimens (n = 230 each): basal diet, 2.5% and 5% FRS from day 28 of life to day 85. The piglets fed with 2.5% FRS presented superior phenotype with alleviated intraepithelial and stromal lymphocytes infiltration in the gut, enhanced colon mucosa barrier as well as numerically improvements of final body weight. Colon microbiota composition was determined using amplicon sequencing of the V3 and V1 – V8 region of the 16S rRNA gene using Illumina Nextseq and Oxford Nanopore MinION sequencing, respectively. The two amplicon sequencing strategies showed high consistence between the detected bacteria. Both sequencing technologies showed that the FRS fed piglets had a distinctly different microbial composition relative to the basal diet. Compared with piglets fed the basal diet, Prevotella stercorea was verified by both technologies to be more abundant in the FRS piglets, and positively correlated with colon mucosa thickness and negatively correlated with blood levels of leucocytes and IgG. In conclusion, FRS supplementation improved gut health of weaner piglets, and altered their gut microbiota composition. Increasing the dietary inclusion of FRS from 2.5% to 5% did not cause further improvements.

Geographical variations in infectivity and susceptibility in the host-parasite system Schistosoma mansoni/Biomphalaria glabrata: no evidence for local adaptation

Parasitology ◽  
2006 ◽  
Vol 133 (3) ◽  
pp. 313-319 ◽  
Author(s):  
F. PRUGNOLLE ◽  
T. DE MEEÛS ◽  
J.P. POINTIER ◽  
P. DURAND ◽  
A. ROGNON ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Local Adaptation ◽  
Genetic Factors ◽  
Biomphalaria Glabrata ◽  
Metapopulation Dynamics ◽  
Geographical Variations ◽  
Transplantation Experiment ◽  
Host Parasite ◽  
Parasite Populations ◽  
Transplantation Experiments

We investigated local adaptation in the spatially structured natural Biomphalaria glabrata/Schistosoma mansoni host-parasite system in the marshy forest focus of Guadeloupe using cross-transplantation experiments. We demonstrated strong and highly significant variations in susceptibility/infectivity of host and parasite populations, respectively, but found no evidence of local adaptation neither for S. mansoni nor for B. glabrata. Environmental as well as genetic factors are discussed to explain susceptibility/infectivity variations between both host and parasite populations. The absence of local adaptation is discussed in relation to the metapopulation dynamics of both host and parasite, in particular their relative rates of dispersal at the scale under scrutiny. Our study constitutes the first cross-transplantation experiment concerning this host-parasite system of which both hosts and parasites came directly from the wild, excluding laboratory generations and experimental host passages.

scholarly journals Physical activity and gut microbiota: a cross-sectional study

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
F Gallè ◽  
F Valeriani ◽  
M Antinozzi ◽  
R Liguori ◽  
G Gianfranceschi ◽  
...  
Keyword(s):  
Physical Activity ◽  
Gut Microbiota ◽  
Amplicon Sequencing ◽  
Cross Sectional Study ◽  
Microbiota Composition ◽  
Sectional Study ◽  
Microbial Composition ◽  
Cross Sectional ◽  
Bacterial Phyla ◽  
Gut Microbiota Composition

Abstract Background The composition of gut microbiota, and in particular the intestinal abundance of the two main bacterial phyla of Firmicutes and Bacteroidetes, are associated with human health and diseases and may be conditioned by host and environmental factors such as age, gender and diet. The role of Physical Activity (PA) in determining gut microbiota composition has not been yet completely clarified. A cross-sectional study involving undergraduates from two Italian cities is ongoing to explore this relationship. Methods Students were invited to provide a fecal sample and to complete the International Physical Activity Questionnaire (IPAQ) in order to define their habitual PA level (inactive, minimally active, health enhancing physical activity -HEPA- active). Demographic and anthropometric information were also collected. DNA from fecal samples was analyzed through the 16S amplicon sequencing. Microbial composition and variability of the samples were evaluated on the light of participants' PA levels. Results A total of 153 students (47.7% males, mean age 22.4±2.9, mean BMI 22.3±2.7) participated to the study so far. Firmicutes and Bacteroidetes were the main represented phyla. An increase in Firmicutes (58.3±16 to 61.4±13.3, p = 0.68) and a reduction in Bacteroidetes (32.6±14.8 to 30.3±11.4, p = 0.51) have been registered with the increase of PA level. A higher variability (expressed as Shannon α-index) has been detected in minimally active (3.39±0.03) and HEPA-active (3.41±0) individuals respect to inactive subjects (3.35±0.07) (p = 0.05). Conclusions Even if they are not significant, these preliminary results suggest a relationship between PA levels and gut microbiota composition. An active lifestyle seems to be associated with a greater microbial diversity in the gut. Further researches are needed to explain these findings. Key messages Physical activity seems to be associated with gut microbiota composition. A greater variability in gut microbiota was found in active people.

scholarly journals Observations on two biological races of Schistosoma mansoni

1981 ◽  
Vol 76 (3) ◽  
pp. 287-291 ◽  
Author(s):  
W. Lobato Paraense ◽  
Lygia R. Corrêa
Keyword(s):  
Comparative Study ◽  
Schistosoma Mansoni ◽  
Biomphalaria Glabrata ◽  
Prepatent Period ◽  
Snail Species ◽  
Infection Rates ◽  
Paulo State ◽  
Minas Gerais State ◽  
Belo Horizonte ◽  
Host Parasite

A comparative study of the BH strain of Schistosoma mansoni from Belo Horizonte, Minas Gerais state, infective to Biomphalaria glabrata from the same locality, and the SJ strain from São José dos Campos, São Paulo state, infective to B. tenagophila from the latter locality, showed the following differences: 1. Length of adult worms and size of eggs significantly larger in the BH strain. 2. Higher infection rates in the B. glabrata-BH strain association than in the B. tenagophila-SJ strain association, following exposure of each snail to 1 or 10 miracidia. 3.Longer prepatent period (from penetration of miracidium to first shedding of cercariae) in the B. tenagophila-SJ strain association. 4. Infection of both Biomphalaria species when exposed to hybrid miracidia from crosses between the two strains, at lower levels than those resulting from exposure of each snail species to miracidia of the pure sympatric strain. (Both Biomphalaria populations are practically refractory to infection with the allopatric strain). These results are interpreted as pointing to a better host-parasite adjustment in the B. glabrata-BH strain association than in the B. tenagophila-SJ association. The interfertility between the two strains, which produced viable hybrids infective to both Biomphalaria species, supports the conclusion that the observed differences are merely intraspecific, and that the two strains may be considered distinct biological races of Schistosoma mansoni.

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