scholarly journals Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar

2021 ◽  
Vol 9 (5) ◽  
pp. 904
Author(s):  
Antonia Touloudi ◽  
George Valiakos ◽  
Shaun Cawthraw ◽  
Polychronis Kostoulas ◽  
Christian Gortázar ◽  
...  
Keyword(s):  
Wild Boar ◽  
Mycobacterium Bovis ◽  
Magnetic Beads ◽  
Trichinella Spiralis ◽  
Simultaneous Detection ◽  
Multiplex Assay ◽  
Diagnostic Potential ◽  
Serological Status ◽  
Brucella Suis ◽  
Multiplex Bead

The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar.

Development of a Multiplex Bead-Based Method for the Microquantitation of δ-Catenin

2020 ◽  
Vol 20 (9) ◽  
pp. 5819-5822
Author(s):  
Mijin Lee ◽  
İsa Taş ◽  
Rui Zhou ◽  
Sang-Chul Jung ◽  
Kwonseop Kim ◽  
...  
Keyword(s):  
Cancer Screening ◽  
Western Blot ◽  
Magnetic Beads ◽  
Diagnostic Marker ◽  
Hek293t Cell ◽  
Multiplex Assay ◽  
Cell Lysates ◽  
Ovarian Cancers ◽  
Multiplex Bead ◽  
Single Reaction

δ-Catenin is overexpressed in human cancers, including prostate, breast, lung, and ovarian cancers. Therefore, detection of δ-catenin level in patient specimens can be used as a diagnostic marker for the cancer screening. In laboratories, δ-catenin levels have been analyzed by western blot, which requires multiple procedures and is incapable of multiplex analysis of a target protein from a single reaction. In this study, we aimed to develop δ-catenin antibody-conjugated magnetic beads that can be used for quantitation of δ-catenin by bead-based multiplex assay. δ-catenin level from HEK293T and CWR22Rv-1 cell lysates was quantified to determine whether the antibody-conjugatedmagnetic bead can be used for the detection of trace amounts of δ-catenin. Our results indicate that analysis with 1 μg of CWR22Rv-1 and HEK293T cell lysates showed 918.67±103.8 and 874.33±37.21 MFI values, respectively. In conclusion, the results suggest that the procedure for detection of δ-catenin level through the antibody-conjugated magnetic beads provides a sensitive and costeffective solution for screening from patient specimens.

scholarly journals Whole Genome Sequencing Refines Knowledge on the Population Structure of Mycobacterium bovis from a Multi-Host Tuberculosis System

2021 ◽  
Vol 9 (8) ◽  
pp. 1585
Author(s):  
Ana C. Reis ◽  
Liliana C. M. Salvador ◽  
Suelee Robbe-Austerman ◽  
Rogério Tenreiro ◽  
Ana Botelho ◽  
...  
Keyword(s):  
Population Structure ◽  
Whole Genome Sequencing ◽  
Wild Boar ◽  
Genome Sequencing ◽  
Mycobacterium Bovis ◽  
Red Deer ◽  
Variable Number Tandem Repeat ◽  
Variant Calling ◽  
Whole Genome ◽  
Network Analyses

Classical molecular analyses of Mycobacterium bovis based on spoligotyping and Variable Number Tandem Repeat (MIRU-VNTR) brought the first insights into the epidemiology of animal tuberculosis (TB) in Portugal, showing high genotypic diversity of circulating strains that mostly cluster within the European 2 clonal complex. Previous surveillance provided valuable information on the prevalence and spatial occurrence of TB and highlighted prevalent genotypes in areas where livestock and wild ungulates are sympatric. However, links at the wildlife–livestock interfaces were established mainly via classical genotype associations. Here, we apply whole genome sequencing (WGS) to cattle, red deer and wild boar isolates to reconstruct the M. bovis population structure in a multi-host, multi-region disease system and to explore links at a fine genomic scale between M. bovis from wildlife hosts and cattle. Whole genome sequences of 44 representative M. bovis isolates, obtained between 2003 and 2015 from three TB hotspots, were compared through single nucleotide polymorphism (SNP) variant calling analyses. Consistent with previous results combining classical genotyping with Bayesian population admixture modelling, SNP-based phylogenies support the branching of this M. bovis population into five genetic clades, three with apparent geographic specificities, as well as the establishment of an SNP catalogue specific to each clade, which may be explored in the future as phylogenetic markers. The core genome alignment of SNPs was integrated within a spatiotemporal metadata framework to further structure this M. bovis population by host species and TB hotspots, providing a baseline for network analyses in different epidemiological and disease control contexts. WGS of M. bovis isolates from Portugal is reported for the first time in this pilot study, refining the spatiotemporal context of TB at the wildlife–livestock interface and providing further support to the key role of red deer and wild boar on disease maintenance. The SNP diversity observed within this dataset supports the natural circulation of M. bovis for a long time period, as well as multiple introduction events of the pathogen in this Iberian multi-host system.

scholarly journals A correlation of Mycobacterium bovis SB0134 infection between cattle and a wild boar (Sus Scrofa) in Campania region

2021 ◽  
pp. 100182
Author(s):  
Emanuela Sannino ◽  
Lorena Cardillo ◽  
Rubina Paradiso ◽  
Anna Cerrone ◽  
Paolo Coppa ◽  
...  
Keyword(s):  
Wild Boar ◽  
Mycobacterium Bovis ◽  
Sus Scrofa ◽  
Campania Region

Multiplex bead-array competitive immunoassay for simultaneous detection of three pesticides in vegetables

2013 ◽  
Vol 180 (5-6) ◽  
pp. 387-395 ◽  
Author(s):  
Yirong Guo ◽  
Jie Tian ◽  
Chizhou Liang ◽  
Guonian Zhu ◽  
Wenjun Gui
Keyword(s):  
Simultaneous Detection ◽  
Competitive Immunoassay ◽  
Multiplex Bead

Gene expression changes in spleens of the wildlife reservoir species, Eurasian wild boar (Sus scrofa), naturally infected with Brucella suis biovar 2

2010 ◽  
Vol 37 (11) ◽  
pp. 725-736 ◽  
Author(s):  
Ruth C. Galindo ◽  
Pilar M. Muñoz ◽  
María J. de Miguel ◽  
Clara M. Marin ◽  
Javier Labairu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Reservoir Species ◽  
Brucella Suis ◽  
Wildlife Reservoir

scholarly journals Monitoring results of wild boar (Sus scrofa) in The Netherlands: analyses of serological results and the first identification of Brucella suis biovar 2

2020 ◽  
Vol 10 (1) ◽  
pp. 1794668
Author(s):  
Peter van Tulden ◽  
Jose L. Gonzales ◽  
Michiel Kroese ◽  
Marc Engelsma ◽  
Frido de Zwart ◽  
...  
Keyword(s):  
The Netherlands ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Brucella Suis

scholarly journals Development of an ultrasensitive multiplex assay for simultaneous detection of Aβ1‐42, Aβ1‐40, GFAP and NF‐L in blood

2020 ◽  
Vol 16 (S4) ◽  
Author(s):  
Dandan Shan ◽  
Marcella Holdridge ◽  
Lei Chang ◽  
Erik Stoops ◽  
Inge M.W. Verberk ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Multiplex Assay

Use of the MILLIPLEX® bovine cytokine/chemokine multiplex assay to identify Mycobacterium bovis-infection biomarkers in African buffaloes (Syncerus caffer)

2021 ◽  
Vol 231 ◽  
pp. 110152
Author(s):  
Katrin Smith ◽  
Léanie Kleynhans ◽  
Candice Snyders ◽  
Netanya Bernitz ◽  
David Cooper ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Multiplex Assay ◽  
Syncerus Caffer

scholarly journals Testing Eurasian wild boar piglets for serum antibodies against Mycobacterium bovis

2015 ◽  
Vol 121 (1-2) ◽  
pp. 93-98 ◽  
Author(s):  
A. Che’ Amat ◽  
D. González-Barrio ◽  
J.A. Ortiz ◽  
I. Díez-Delgado ◽  
M. Boadella ◽  
...  
Keyword(s):  
Wild Boar ◽  
Mycobacterium Bovis ◽  
Serum Antibodies
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