scholarly journals Microbial Succession under Freeze–Thaw Events and Its Potential for Hydrocarbon Degradation in Nutrient-Amended Antarctic Soil

2021 ◽  
Vol 9 (3) ◽  
pp. 609
Author(s):  
Hugo Emiliano de Jesus ◽  
Renato S. Carreira ◽  
Simone S. M. Paiva ◽  
Carlos Massone ◽  
Alex Enrich-Prast ◽  
...  

The polar regions have relatively low richness and diversity of plants and animals, and the basis of the entire ecological chain is supported by microbial diversity. In these regions, understanding the microbial response against environmental factors and anthropogenic disturbances is essential to understand patterns better, prevent isolated events, and apply biotechnology strategies. The Antarctic continent has been increasingly affected by anthropogenic contamination, and its constant temperature fluctuations limit the application of clean recovery strategies, such as bioremediation. We evaluated the bacterial response in oil-contaminated soil through a nutrient-amended microcosm experiment using two temperature regimes: (i) 4 °C and (ii) a freeze–thaw cycle (FTC) alternating between −20 and 4 °C. Bacterial taxa, such as Myxococcales, Chitinophagaceae, and Acidimicrobiales, were strongly related to the FTC. Rhodococcus was positively related to contaminated soils and further stimulated under FTC conditions. Additionally, the nutrient-amended treatment under the FTC regime enhanced bacterial groups with known biodegradation potential and was efficient in removing hydrocarbons of diesel oil. The experimental design, rates of bacterial succession, and level of hydrocarbon transformation can be considered as a baseline for further studies aimed at improving bioremediation strategies in environments affected by FTC regimes.

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Qiang Wang ◽  
Jinyang Cui

Cement solidification/stabilization is a commonly used method for the remediation of contaminated soils. The stability characteristics of solidified/stabilized contaminated soils under freeze-thaw cycle are very important. A series of tests, which include unconfined compressive strength tests, freeze-thaw cycle tests, and scanning electron microscopy (SEM) tests, are performed to study the variation law of strength characteristics and microstructure. It aims at revealing the microcosmic mechanism of solidified/stabilized Pb2+ contaminated soils with cement under freeze-thaw cycle. The results show that the unconfined compressive strength of the contaminated soils significantly improved with the increase of the cement content. The unconfined compressive strength of stabilized contaminated soils first increases with the increase of times of freeze-thaw cycle, and after reaching the peak, it decreases with the increase of times of freeze-thaw cycle. The results of the scanning electron microscopy tests are consistent with those of the unconfined compressive strength tests. This paper also reveals the microcosmic mechanism of the changes in engineering of the stabilized contaminated soils under freeze-thaw cycle.


Author(s):  
Zhongping Yang ◽  
Yao Wang ◽  
Denghua Li ◽  
Xuyong Li ◽  
Xinrong Liu

The solidification/stabilization (S/S) method is the usual technique for the remediation of soils polluted by heavy metal in recent years. However, freeze–thaw cycles, an important physical process producing weathering of materials, will affect the long-term stability of engineering characteristics in solidified contaminated soil. In addition, it is still questionable whether using large dosages of binders can enhance the engineering properties of solidified/stabilized contaminated soils. In this study, the three most commonly used binders (i.e., cement, quicklime, and fly ash), alone and mixed in different ratios, were thus added to lead-contaminated soil in various dosages, making a series of cured lead-contaminated soils with different dosages of binders. Afterward, unconfined compression strength tests, direct shear tests, and permeability tests were employed on the resulting samples to find the unconfined compressive strength (UCS), secant modulus ( E 50 ), internal friction angle ( φ ), cohesion ( c ), and permeability coefficient ( k ) of each solidified/stabilized lead-contaminated soil after 0, 3, 7, and 14 days of freeze–thaw cycles. This procedure was aimed at evaluating the influence of freeze–thaw cycle and binder dosage on engineering properties of solidified/stabilized lead-contaminated soils. Results of our experiments showed that cement/quicklime/fly ash could remediate lead-contaminated soils. However, it did not mean that the more the dosage of binder, the better the curing effect. There was a critical dosage. Excessive cementation of contaminated soils caused by too much binder would result in loss of strength and an increase in permeability. Furthermore, it was found that UCS,   E 50 , φ , c , and k values generally decreased with the increase in freeze–thaw cycle time—a deterioration effect on the engineering characteristics of solidified lead-contaminated soils. Avoiding excessive cementation, 2.5% cement or quicklime was favorable for the value of E 50 while a 2.5% fly ash additive was beneficial for the k value. It is also suggested that if the freeze–thaw cycle continues beyond the period supported by excessive cementation, such a cycle will rapidly destroy the original structure of the soil and create large cracks, leading to an increase in permeability. The results also showed that the contaminated soils with a larger dosage of binders exhibited more significant deterioration during freeze–thaw cycles.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Longxiao Chen ◽  
Kesheng Li ◽  
Guilei Song ◽  
Deng Zhang ◽  
Chuanxiao Liu

AbstractRock deterioration under freeze–thaw cycles is a concern for in-service tunnel in cold regions. Previous studies focused on the change of rock mechanical properties under unidirectional stress, but the natural rock mass is under three dimensional stresses. This paper investigates influences of the number of freeze–thaw cycle on sandstone under low confining pressure. Twelve sandstone samples were tested subjected to triaxial compression. Additionally, the damage characteristics of sandstone internal microstructure were obtained by using acoustic emission (AE) and mercury intrusion porosimetry. Results indicated that the mechanical properties of sandstone were significantly reduced by freeze–thaw effect. Sandstone’ peak strength and elastic modulus were 7.28–37.96% and 6.38–40.87% less than for the control, respectively. The proportion of super-large pore and large pore in sandstone increased by 19.53–81.19%. We attributed the reduced sandstone’ mechanical properties to the degenerated sandstone microstructure, which, in turn, was associated with increased sandstone macropores. The macroscopic failure pattern of sandstone changed from splitting failure to shear failure with an increasing of freeze–thaw cycles. Moreover, the activity of AE signal increased at each stage, and the cumulative ringing count also showed upward trend with the increase of freeze–thaw number.


2021 ◽  
Vol 28 (3) ◽  
pp. 954-967
Author(s):  
Jie-lin Li ◽  
Long-yin Zhu ◽  
Ke-ping Zhou ◽  
Hui Chen ◽  
Le Gao ◽  
...  

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Huren Rong ◽  
Jingyu Gu ◽  
Miren Rong ◽  
Hong Liu ◽  
Jiayao Zhang ◽  
...  

In order to study the damage characteristics of the yellow sandstone containing pores under the freeze-thaw cycle, the uniaxial compression test of saturated water-stained yellow sandstones with different freeze-thaw cycles was carried out by rock servo press, the microstructure was qualitatively analyzed by Zeiss 508 stereo microscope, and the microdamage mechanism was quantitatively studied by using specific surface area and pore size analyzer. The mechanism of weakening mechanical properties of single-hole yellow sandstone was expounded from the perspective of microstructure. The results show the following. (1) The number of freeze-thaw cycles and single-pore diameter have significant effects on the strength and elastic modulus of the yellow sandstone; the more the freeze-thaw cycles and the larger the pore size, the lower the strength of the yellow sandstone. (2) The damage modes of the yellow sandstone containing pores under the freeze-thaw cycle are divided into five types, and the yellow sandstone with pores is divided into two areas: the periphery of the hole and the distance from the hole; as the number of freeze-thaw cycles increases, different regions show different microscopic damage patterns. (3) The damage degree of yellow sandstone is different with freeze-thaw cycle and pore size. Freeze-thaw not only affects the mechanical properties of yellow sandstone but also accelerates the damage process of pores. (4) The damage of the yellow sandstone by freeze-thaw is logarithmic function, and the damage of the yellow sandstone is a power function. The damage equation of the yellow sandstone with pores under the freezing and thawing is a log-power function nonlinear change law and presents a good correlation.


2012 ◽  
Vol 455-456 ◽  
pp. 781-785
Author(s):  
Ping Lu ◽  
Xin Mao Li ◽  
Xue Qiang Ma ◽  
Wei Bo Huang

. This paper mainly studied the properties of PAE polyurea coated concrete under coactions of salt fog and freeze-thaw. After exposed salt fog conditions for 200d, T3, B2, F2 and TM four coated concrete relative dynamic elastic modulus have small changes, but different coated concrete variation amplitude is different. T3 coated concrete after 100 times of freeze-thaw cycle the relative dynamic elastic modulus began to drop, 200 times freeze-thaw cycle ends, relative dynamic elastic modulus variation is the largest, decrease rate is 95%, TM concrete during 200 times freeze-thaw cycle, relative dynamic elastic modulus almost no change, B2 concrete and F2 concrete the extent of change between coating T3 and TM. After 300 times the freeze-thaw cycle coated concrete didn't appear freeze-thaw damage phenomenon. Four kinds of coating concrete relative dynamic elastic modulus variation by large to small order: T3 coated concrete > B2 coated concrete >F2 coated concrete > TM coated concrete, concrete with the same 200d rule. Frost resistance order, by contrast, TM coated concrete > B2 coated concrete > F2 coated concrete > T3 coated concrete.


2005 ◽  
Vol 27 (9) ◽  
pp. 655-660 ◽  
Author(s):  
Dong-Wook Han ◽  
Hak Hee Kim ◽  
Mi Hee Lee ◽  
Hyun Sook Baek ◽  
Kwon-Yong Lee ◽  
...  

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