scholarly journals Soil Bacterial Diversity Is Positively Correlated with Decomposition Rates during Early Phases of Maize Litter Decomposition

2021 ◽  
Vol 9 (2) ◽  
pp. 357
Author(s):  
Akane Chiba ◽  
Yoshitaka Uchida ◽  
Susanne Kublik ◽  
Gisle Vestergaard ◽  
Franz Buegger ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Litter Decomposition ◽  
Community Dynamics ◽  
Rrna Gene ◽  
Soil Bacterial Diversity ◽  
Decomposition Rates ◽  
Oligotrophic Bacteria ◽  
Soil Bacterial ◽  
Early Phases ◽  
Copiotrophic Bacteria

This study aimed to investigate the effects of different levels of soil- and plant-associated bacterial diversity on the rates of litter decomposition, and bacterial community dynamics during its early phases. We performed an incubation experiment where soil bacterial diversity (but not abundance) was manipulated by autoclaving and reinoculation. Natural or autoclaved maize leaves were applied to the soils and incubated for 6 weeks. Bacterial diversity was assessed before and during litter decomposition using 16S rRNA gene metabarcoding. We found a positive correlation between litter decomposition rates and soil bacterial diversity. The soil with the highest bacterial diversity was dominated by oligotrophic bacteria including Acidobacteria, Nitrospiraceae, and Gaiellaceae, and its community composition did not change during the incubation. In the less diverse soils, those taxa were absent but were replaced by copiotrophic bacteria, such as Caulobacteraceae and Beijerinckiaceae, until the end of the incubation period. SourceTracker analysis revealed that litter-associated bacteria, such as Beijerinckiaceae, only became part of the bacterial communities in the less diverse soils. This suggests a pivotal role of oligotrophic bacteria during the early phases of litter decomposition and the predominance of copiotrophic bacteria at low diversity.

scholarly journals Soil Bacterial Diversity Screening Using Single 16S rRNA Gene V Regions Coupled with Multi-Million Read Generating Sequencing Technologies

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e42671 ◽  
Author(s):  
Sotirios Vasileiadis ◽  
Edoardo Puglisi ◽  
Maria Arena ◽  
Fabrizio Cappa ◽  
Pier S. Cocconcelli ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Diversity ◽  
Rrna Gene ◽  
Soil Bacterial Diversity ◽  
Sequencing Technologies ◽  
Soil Bacterial

scholarly journals TRFLP Analysis of the Effect of a Forest-to-Gap Ectone on Soil Microbial Diversity

2014 ◽  
Author(s):  
Ronald Smith
Keyword(s):  
Microbial Diversity ◽  
Bacterial Diversity ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Restriction Enzyme Digestion ◽  
Dna Fragments ◽  
Soil Bacterial Diversity ◽  
Soil Microbial Diversity ◽  
Soil Microbial ◽  
Soil Bacterial

<p>To investigate how soil microbial diversity is influenced by the formation of an experimental edge-creating gap within a southern New England oak-hickory forest, I used a molecular fingerprinting technique known as terminal restriction fragment length polymorphism (TRFLP). Sequence variability in the 16S ribosomal RNA (rRNA) gene in soil bacterial communities is detected by differences in the length and abundance of fragments produced by digesting PCR products amplified from rRNA genes. The different patterns observed are assumed to represent unique phylotypes.</p> <p>How does the forest-gap ecotone influence soil bacterial diversity? Based on other studies, I hypothesized that the edge would contain the most diverse bacterial community, followed by the gap, and as distance from the gap into the forest increased, bacterial diversity would decrease.</p> <p>Soil samples were collected along 40m transects perpendicular to the northern edge of the gap. DNA was extracted from each sample, PCR was used to amplify the 16S rRNA gene, and DNA fragments were cut by restriction enzyme digestion and separated via electrophoresis. The DNA fragments were analyzed by TRFLP.</p> <p>Environmental variables (soil pH, soil temperature and soil gravimetric moisture) were different in the gap compared to all other distances, but not significantly so. Phylotype richness and diversity (Simpson’s index) was greatest at the edge. Canonical Correspondence Analysis (CCA) produced a phylotype-by-distance ordination that supports my hypothesis and shows that the edge is an ecotone (transitional zone) between the gap and forest.</p>

scholarly journals Effects of Simulated Nitrogen Deposition on the Bacterial Community of Urban Green Spaces

2021 ◽  
Vol 11 (3) ◽  
pp. 918
Author(s):  
Lingzi Mo ◽  
Augusto Zanella ◽  
Xiaohua Chen ◽  
Bin Peng ◽  
Jiahui Lin ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Diversity ◽  
Negative Impact ◽  
Bacterial Community Composition ◽  
N Deposition ◽  
Soil Bacterial Community ◽  
Rrna Gene ◽  
Soil Dna ◽  
Urban Green ◽  
Soil Bacterial

Continuing nitrogen (N) deposition has a wide-ranging impact on terrestrial ecosystems. To test the hypothesis that, under N deposition, bacterial communities could suffer a negative impact, and in a relatively short timeframe, an experiment was carried out for a year in an urban area featuring a cover of Bermuda grass (Cynodon dactylon) and simulating environmental N deposition. NH4NO3 was added as external N source, with four dosages (N0 = 0 kg N ha−2 y−1, N1 = 50 kg N ha−2 y−1, N2 = 100 kg N ha−2 y−1, N3 = 150 kg N ha−2 y−1). We analyzed the bacterial community composition after soil DNA extraction through the pyrosequencing of the 16S rRNA gene amplicons. N deposition resulted in soil bacterial community changes at a clear dosage-dependent rate. Soil bacterial diversity and evenness showed a clear trend of time-dependent decline under repeated N application. Ammonium nitrogen enrichment, either directly or in relation to pH decrease, resulted in the main environmental factor related to the shift of taxa proportions within the urban green space soil bacterial community and qualified as a putative important driver of bacterial diversity abatement. Such an impact on soil life induced by N deposition may pose a serious threat to urban soil ecosystem stability and surrounding areas.

The effects of different vegetation restoration patterns on soil bacterial diversity for sandy land in Hulunbeier

2013 ◽  
Vol 33 (4) ◽  
pp. 211-216 ◽  
Author(s):  
Haifang Zhang ◽  
Xiaolong Song ◽  
Cailing Wang ◽  
Hongmei Liu ◽  
Jingni Zhang ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Vegetation Restoration ◽  
Sandy Land ◽  
Soil Bacterial Diversity ◽  
Soil Bacterial

Sheep grazing and soil bacterial diversity in shrublands of the Patagonian Monte, Argentina

2016 ◽  
Vol 125 ◽  
pp. 16-20 ◽  
Author(s):  
Nelda L. Olivera ◽  
Luciano Prieto ◽  
Mónica B. Bertiller ◽  
Marcela A. Ferrero
Keyword(s):  
Bacterial Diversity ◽  
Sheep Grazing ◽  
Soil Bacterial Diversity ◽  
Soil Bacterial
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