scholarly journals In Vivo Endophytic, Rhizospheric and Epiphytic Colonization of Vitis vinifera by the Plant-Growth Promoting and Antifungal Strain Pseudomonas protegens MP12

2021 ◽  
Vol 9 (2) ◽  
pp. 234
Author(s):  
Marco Andreolli ◽  
Giacomo Zapparoli ◽  
Silvia Lampis ◽  
Chiara Santi ◽  
Elisa Angelini ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Materials ◽  
Detached Leaves ◽  
Young Leaves ◽  
Plant Growth Promoting ◽  
Microscopy Analysis ◽  
Growth Promoting ◽  
The Stability ◽  
Nursery Field

An evaluation was conducted of the colonization of Pseudomonas protegens MP12, a plant-growth promoting and antagonistic strain, inoculated in vine plants during a standard process of grapevine nursery propagation. Three in vivo inoculation protocols (endophytic, rhizospheric, and epiphytic) were implemented and monitored by means of both culture-dependent and independent techniques. Endophytic treatment resulted in the colonization of the bacterium inside the vine cuttings, which spread to young leaves during the forcing period. Microscopy analysis performed on transformed dsRed-tagged P. protegens MP12 cells confirmed the bacterium’s ability to penetrate the inner part of the roots. However, endophytic MP12 strain was no longer detected once the plant materials had been placed in the vine nursery field. The bacterium also displayed an ability to colonize the rhizosphere and, when the plants were uprooted at the end of the vegetative season, its persistence was confirmed. Epiphytic inoculation, performed by foliar spraying of cell suspension, was effective in controlling artificially-induced Botrytis cinerea infection in detached leaves. The success of rhizospheric and leaf colonization in vine plants suggests potential for the future exploitation of P. protegens MP12 as biofertilizer and biopesticide. Further investigation is required into the stability of the bacterium’s colonization of vine plants under real-world conditions in vineyards.

In Vitro and In Vivo Characterization of Plant Growth Promoting Bacillus Strains Isolated from Extreme Environments of Eastern Algeria

2013 ◽  
Vol 172 (4) ◽  
pp. 1735-1746 ◽  
Author(s):  
Asma Ait-Kaki ◽  
Noreddine Kacem-Chaouche ◽  
Marc Ongena ◽  
Mounira Kara-Ali ◽  
Laid Dehimat ◽  
...  
Keyword(s):  
Plant Growth ◽  
Extreme Environments ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Eastern Algeria ◽  
In Vivo Characterization ◽  
Bacillus Strains

Plant growth promoting fluorescent Pseudomonas enhancing growth of sunflower crop

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Sakshi Tewari ◽  
Naveen Kumar Arora
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Phosphate Solubilization ◽  
Dry Weight ◽  
Fluorescent Pseudomonas ◽  
Plant Growth Promoting ◽  
Sunflower Crop ◽  
Growth Promoting ◽  
Semi Arid Region

<p>Ten bacterial isolates were obtained from the rhizosphere of sunflower crop grown in the semi-arid region of west Kanpur.<br />Isolates were further characterized on the basis of morphological, biochemical and physiological characteristics<br />suggesting them to be the member of group fluorescent pseudomonas. Isolates were further monitored for plant growth<br />promoting traits including IAA, phosphate solubilization, siderophore, nitrogen fixation, HCN, chitinase and β-1-3<br />glucanase activity. Amongst all the isolates, PF17 displayed maximum PGP attributes hence it was selected for doing<br />further in vivo pot study taking sunflower as a test crop. Seed treatment with fluorescent pseudomonas PF17 brought<br />enhancement in root length, shoot length, dry weight and seed yield of sunflower crop in comparison to control (untreated<br />seeds). Hence it might be concluded from the study that fluorescent pseudomonas PF17 contains large number of PGP<br />attributes, and its application contributed in enhancement of sunflower growth leading to better yield. In addition, ability<br />to enhance growth of sunflower with the help of biological means appears to be of great ecological and economic<br />importance.</p>

scholarly journals Evaluation of Plant Growth Promoting Ability of <i>Bacillus amyloliquefaciens</i> Bc2 and <i>Trichoderma harzianum</i> TR <i>In Vivo</i>

2020 ◽  
Vol 11 (03) ◽  
pp. 247-259
Author(s):  
Rababe Es-Soufi ◽  
Houda Tahiri ◽  
Aïcha El Oualkadi ◽  
Latifa Azaroual ◽  
Patrick Martin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Trichoderma Harzianum ◽  
Bacillus Amyloliquefaciens ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant growth promoting endophyte Burkholderia contaminans NZ antagonizes phytopathogen Macrophomina phaseolina through melanin synthesis and pyrrolnitrin inhibition

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257863
Author(s):  
Nazia R. Zaman ◽  
Umar F. Chowdhury ◽  
Rifath N. Reza ◽  
Farhana T. Chowdhury ◽  
Mrinmoy Sarker ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Genome Mining ◽  
Gene Clusters ◽  
Macrophomina Phaseolina ◽  
Melanin Synthesis ◽  
Burkholderia Contaminans ◽  
Plant Growth Promoting ◽  
Growth Promoting

The endophytic bacterium Burkholderia contaminans NZ was isolated from jute, which is an important fiber-producing plant. This bacterium exhibits significant growth promotion activity in in vivo pot experiments, and like other plant growth-promoting (PGP) bacteria fixes nitrogen, produces indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. B. contaminans NZ is considered to exert a promising growth inhibitory effect on Macrophomina phaseolina, a phytopathogen responsible for infecting hundreds of crops worldwide. This study aimed to identify the possibility of B. contaminans NZ as a safe biocontrol agent and assess its effectiveness in suppressing phytopathogenic fungi, especially M. phaseolina. Co-culture of M. phaseolina with B. contaminans NZ on both solid and liquid media revealed appreciable growth suppression of M. phaseolina and its chromogenic aberration in liquid culture. Genome mining of B. contaminans NZ using NaPDoS and antiSMASH revealed gene clusters that displayed 100% similarity for cytotoxic and antifungal substances, such as pyrrolnitrin. GC-MS analysis of B. contaminans NZ culture extracts revealed various bioactive compounds, including catechol; 9,10-dihydro-12’-hydroxy-2’-methyl-5’-(phenylmethyl)- ergotaman 3’,6’,18-trione; 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one; 1-(1,6-Dioxooctadecyl)- pyrrolidine; 9-Octadecenamide; and 2- methoxy- phenol. These compounds reportedly exhibit tyrosinase inhibitory, antifungal, and antibiotic activities. Using a more targeted approach, an RP-HPLC purified fraction was analyzed by LC-MS, confirming the existence of pyrrolnitrin in the B. contaminans NZ extract. Secondary metabolites, such as catechol and ergotaman, have been predicted to inhibit melanin synthesis in M. phaseolina. Thus, B. contaminans NZ appears to inhibit phytopathogens by apparently impairing melanin synthesis and other potential biochemical pathways, exhibiting considerable fungistatic activity.

Enhancement of verticillium wilt resistance in tomato transplants by in vitro co-culture of seedlings with a plant growth promoting rhizobacterium (Pseudomonas sp. strain PsJN)

1998 ◽  
Vol 44 (6) ◽  
pp. 528-536 ◽  
Author(s):  
V K Sharma ◽  
J Nowak
Keyword(s):  
Plant Growth ◽  
Verticillium Wilt ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Pseudomonas Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

The potential utilization of a plant growth promoting rhizobacterium, Pseudomonas sp. strain PsJN, to enhance the resistance of tomato transplants to verticillium wilt was investigated. Plant growth and disease development were tested on the disease-susceptible cultivar Bonny Best after Verticillium dahliae infection of tissue culture plantlets bacterized in vitro (by co-culturing with the bacterium) and seedlings bacterized in vivo (after 3 weeks growth in the greenhouse). Significant differences in both disease suppression and plant growth were obtained between in vitro bacterized and nonbacterized (control) plants. The degree of protection afforded by in vitro bacterization depended on the inoculum density of V. dahliae; the best and worst protection occurred at the lowest (103 conidia ·mL-1) and highest (106 conidia ·mL-1) levels, respectively. In contrast, the in vivo bacterized tomatoes did not show plant growth promotion when compared to the nonbacterized control plants. When challenged with Verticillium, significant growth differences between in vivo bacterized plants (26.8% for shoot height) and nonbacterized controls were only seen at the 3rd week after inoculation. Compared with the in vitro inoculation, there was no delay in the verticillium wilt symptom expression, even at the lowest concentration of V. dahliae, by in vivo PsJN inoculation. These results suggest that endophytic colonization of tomato tissues is required for the Verticillium-resistance responses. Plant growth promotion preceeds the disease-resistance responses and may depend on the colonization thresholds and subsequent sensitization of hosts.Key words: Pseudomonas sp., plant growth promoting rhizobacterium, Verticillium dahliae, tomato, colonization, plant growth promotion, disease suppression.

Compatibility of plant growth promoting rhizobacterial strains with agrichemicals applied to seed

1992 ◽  
Vol 38 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Robert M. Zablotowicz ◽  
Caroline M. Press ◽  
Nicola Lyng ◽  
Gerry L. Brown ◽  
Joseph W. Kloepper
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth Stimulation ◽  
Seed Treatments ◽  
Greenhouse Study ◽  
Select Group ◽  
Plant Growth Promoting ◽  
Growth Promoting

The compatibility of a select group of plant growth promoting rhizobacterial strains with chemicals commonly used as seed treatments was investigated. Strains in several genera (Serratia, Pseudomonas, and coryneform-like bacteria) were found to be tolerant to Vitavax RS (containing lindane, carboxin, and thiram), Epic (iprodione), and (or) captan tested in vitro at commercial rates. Six of 10 strains survived equally, and exhibited similar root colonization, on Vitavax RS treated and nontreated seed. Four of seven strains tested (Serratia spp. and P. fluorescens) were likewise found to be compatible with a captan seed treatment on supersweet corn, using the same criteria. Ability of bacteria to grow on pesticide-amended media did not always indicate compatibility with chemical seed treatments in vivo. A greenhouse study demonstrated that enhanced emergence occurred with the coryneform-like strain 44-9 on Vitavax RS treated canola seed grown under conditions favoring disease due to Rhizoctonia solani. The ability to combine plant growth promoting rhizobacterial strains with current agrichemicals for plant growth stimulation and disease control is indicated. Key words: pesticide compatibility, Pseudomonas, agrichemicals, Serratia, damping-off, plant growth promoting rhizobacteria.

scholarly journals Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum

2021 ◽  
Vol 6 (2) ◽  
pp. 255-263
Author(s):  
Indah Juwita Sari ◽  
Indria Wahyuni ◽  
Rida Oktorida Khastini ◽  
Ewi Awaliyati ◽  
Andriana Susilowati ◽  
...  
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting Bacteria ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.

scholarly journals Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Lamia AOUAR ◽  
INAS BOUKELLOUL ◽  
ABDERRAHMANE BENADJILA
Keyword(s):  
Plant Growth ◽  
Phytopathogenic Fungi ◽  
Growth Promoter ◽  
Bioactive Substances ◽  
Plant Growth Promoting ◽  
Streptomyces Scabiei ◽  
Growth Promoting ◽  
Antagonistic Streptomyces

Abstract. Aouar L, Boukelloul I, Benadjila A. 2020. Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties. Biodiversitas 21: 5672-5683. To produce new bioactive substances of agricultural interest, extreme ecosystems can be a source of unexplored microorganisms. Accordingly, in this study, twenty-two actinobacteria strains were obtained from rhizospheric arid soils of palm groves collected from Biskra and El Oued in the Algerian Sahara. All isolates were examined for the in vitro antifungal potential towards phytopathogenic fungi: Aspergillus flavus, Verticillium dahlia, Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum as well as for their antibacterial property toward phytopathogenic bacteria: Streptomyces scabiei, Pectobacterium carotovorum and Agrobacterium tumefaciens. The three isolates (13%) that inhibited at least five pathogens were then selected, identified and assessed for their attributes to produce indole-3-acetic acid (IAA) and siderophores, to solubilize phosphate, and to antagonize Streptomyces scabiei in vivo. According to phylogenetic analysis performed with 16S rDNA sequence, chemotaxonomy and phenotypic characteristics, the strain SO1, which inhibited all tested pathogens, was assigned to Streptomyces flaveus. While, strains SO2 and SB1 were affiliated to Streptomyces enissocaesilis and Streptomyces albidoflavus, respectively. All strains produced IAA but only SO1 and SB1 were able to elaborate siderophores catecholate-type. Two strains SO1 and SO2 exhibited a capacity to solubilize phosphate and SO1 was able to suppress the pathogenic effect of Streptomyces scabiei on radish seedlings. The findings indicate that SO1 strain may reveal the potential for use as a biocontrol agent and plant growth promoter.

scholarly journals Exploration of Rhizobacteria as Bioagents against Phytophthora Blight and Yield Attributes of Pigeonpea (Cajanus cajan L.): In vitro and In vivo Study

2021 ◽  
pp. 25-33
Author(s):  
Saroj Bala ◽  
Rajni Devi ◽  
Veena Khanna
Keyword(s):  
Plant Growth ◽  
Cajanus Cajan ◽  
Biochemical Characterization ◽  
Growth Parameters ◽  
Pigeon Pea ◽  
Yield Attributes ◽  
Plant Growth Promoting ◽  
Growth Promoting

The latest soil management scenario is occupied by destructive chemical fertilizers, which is a serious risk to both human health as well as to the environment. Advantageous microbes present in soil are used as a biofertilizers for a promising role in sustainable agriculture. Pigeon pea (Cajanus cajan L.) is a primitive protein rich leguminous pulse in India. Thirty-five isolates from rhizospheric soil samples were collected from twelve different locations of Punjab (India). Morphological and biochemical characterization for selection of potential plant growth promoting traits with antifungal properties was undertaken. Most of the inoculated seeds with rhizoisolates evolved a significant increase in growth parameters of pigeon pea as compared to uninoculated seeds, both in vitro and in vivo conditions. Plant growth promoting rhizobacterias (PGPRs) are environmentally safe as they lead to increased production and resistance against diseases of crops.

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