scholarly journals Effects of Ferulic Acid Esterase-Producing Lactic Acid Bacteria and Storage Temperature on the Fermentation Quality, In Vitro Digestibility and Phenolic Acid Extraction Yields of Sorghum (Sorghum bicolor L.) Silage

2021 ◽  
Vol 9 (1) ◽  
pp. 114
Author(s):  
Yixiao Xie ◽  
Jingui Guo ◽  
Wenqi Li ◽  
Zhe Wu ◽  
Zhu Yu
Keyword(s):  
Lactic Acid ◽  
High Temperature ◽  
Phenolic Acid ◽  
In Vitro Digestibility ◽  
Acid Extraction ◽  
Ferulic Acid Esterase ◽  
Fermentation Quality ◽  
Sorghum Silage ◽  
Sorghum Bicolor L

Two lactic acid bacteria (LAB) strains with different ferulic acid esterase (FAE) activities were isolated: Lactobacillus farciminis (LF18) and Lactobacillus plantarum (LP23). The effects of these strains on the fermentation quality, in vitro digestibility and phenolic acid extraction yields of sorghum (Sorghum bicolor L.) silage were studied at 20, 30 and 40 °C. Sorghum was ensiled with no additive (control), LF18 or LP23 for 45 days. At 40 °C, the lactic acid content decreased, whereas the ammonia nitrogen (NH3-N) content significantly increased (p < 0.05). At all three temperatures, the inoculants significantly improved the lactic acid contents and reduced the NH3-N contents (p < 0.05). Neither LP23 nor LF18 significantly improved the digestibility of sorghum silages (p > 0.05). The LP23 group exhibited higher phenolic acid extraction yields at 30 °C (p < 0.05), and the corresponding yields of the LF18 and control groups were improved at 40 °C (p < 0.05). FAE-producing LABs might partially ameliorate the negative effects of high temperature and improve the fermentation quality of sorghum silage. The screened FAE-producing LABs could be candidate strains for preserving sorghum silage at high temperature, and some further insights into the relationship between FAE-producing LABs and ensiling temperatures were obtained.

scholarly journals Effects of fibrolytic enzymes and lactic acid bacteria on fermentation quality and in vitro digestibility of Napier grass silage

2019 ◽  
Vol 18 (1) ◽  
pp. 1438-1444 ◽  
Author(s):  
Smerjai Bureenok ◽  
Sioudome Langsoumechai ◽  
Nittaya Pitiwittayakul ◽  
Chalermpon Yuangklang ◽  
Kraisit Vasupen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Napier Grass ◽  
In Vitro Digestibility ◽  
Fibrolytic Enzymes ◽  
Grass Silage ◽  
Fermentation Quality

scholarly journals Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Fermentation Quality ◽  
Tetraploid Black Locust

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

scholarly journals Effects of Adding Pre-Fermented Fluid Prepared from Red Clover or Lucerne on Fermentation Quality and in Vitro Digestibility of Red Clover and Lucerne Silages

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 454
Author(s):  
Lin Sun ◽  
Yun Jiang ◽  
Qinyin Ling ◽  
Na Na ◽  
Haiwen Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Red Clover ◽  
Ammonia Nitrogen ◽  
In Vitro Digestibility ◽  
Fermentation Quality ◽  
Fermentation Parameters ◽  
Positive Effect

This study examined the effects of chopping or chopping + blender maceration of red clover or lucerne on pre-fermented juice (PFJ) and determined the effects of PFJs on the quality of red clover silage or lucerne silage. The PFJs from chopping red clover (PFJ-RC) or lucerne (PFJ-LC) had a higher lactic acid bacteria (LAB) count than that from chopping + blender maceration (p < 0.05) and were used as additives. Compared with the Control of both silages, adding PFJ increased LAB, lactic acid (LA), and in vitro digestibility of dry matter (IVDMD) (p < 0.05), while pH, acetic acid (AA), and ammonia nitrogen/total nitrogen (NH3-N/TN) were decreased (p < 0.05). For red clover silages, the PFJ-RC treatment contained the greatest LAB and LA and the lowest pH and NH3-N/TN among treatments (p < 0.05); similar results were observed in PFJ-LC treatment for lucerne silages (p < 0.05). The IVDMD of both silages correlated negatively with pH, AA, and NH3-N/TN and positively with LA (p < 0.05). Overall, chopping alone was a better method for preparing PFJ. Adding PFJ at ensiling increased LA and decreased the pH, AA, and NH3-N/TN of both silages. Ensiling lucerne or red clover with PFJ from the ensiling material had a more positive effect on the fermentation parameters mentioned above. Satisfactory fermentation parameters detected in the present study contributed to improving the IVDMD of both silages.

scholarly journals Evaluasi Kualitas, Karakteristik Fermentasi dan Kecernaan In Vitro Silase Campuran Sorgum Stay Green-Gliricidia sepium dengan Penambahan Berbagai Level Asam Laktat

2019 ◽  
Vol 19 (2) ◽  
pp. 99-106
Author(s):  
Widhi Kurniawan ◽  
Syamsuddin Syamsuddin ◽  
Wa Laili Salid ◽  
Purnaning Dhian Isnaini
Keyword(s):  
Lactic Acid ◽  
Sorghum Bicolor ◽  
Crude Protein ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Gliricidia Sepium ◽  
In Vitro Digestibility ◽  
Stay Green ◽  
Sorghum Bicolor L

ABSTRAK. Silase gabungan rumput-leguminosa merupakan inovasi yang dapat diaplikasikan untuk menyediakan pakan dan bisa digunakan sepanjang tahun. Kandungan protein silase campuran tersebut dapat mencapai tingkat optimal sebagai pakan konsentrat hijau (green concentrate). Penelitian ini bertujuan untuk mengevaluasi kualitas, karakter fermentasi dan kecernaan in vitro silase campuran sorgum (Sorghum bicolor (L.) Moench) and gamal (Gliricidia sepium) dengan menambahkan dosis asam laktat yang berbeda. Silase dibuat dengan mencampur bahan penyusun yang terdiri dari sorgum dan daun gamal dengan perbandingan 1:1 dan penambahan asam laktat konsentrasi 20% (v/v). Penelitian terdiri atas 3 perlakuan yaitu; A0: (kontrol) tanpa penambahan asam laktat, A1: penambahan asam laktat sebanyak 2% (b/v), A2: 2,5% (b/v), dan A3: 3% (b/v) dengan 4 ulangan tiap perlakuan. Proses ensilase menggunakan cairan rumen kambing selama 21 hari. Parameter penelitian yang diamati adalah kualitas silase dan kecernaan in vitro. Hasil penelitian menunjukkan bahwa dosis penambahan asam laktat sebanyak 2% menghasilkan silase paling optimal dengan pH (3,94), bahan kering (26,62%), dan nilai Fleigh (100,89), namun tidak berpengaruh terhadap kandungan protein kasar, kecernaan bahan organik, kecernaan bahan kering dan kecernaan protein kasar. Kesimpulan pada penelitian ini adalah penggunaan asam laktat organik dengan dosis 2 hingga 3% (b/v) konsentrasi 20% (v/v) mampu meningkatkan karakteristik, kualitas fermentasi tetapi tidak mempengaruhi nilai KcBK, KcBO dan KcPK silase campuran sorgum stay green (Sorghum bicolor (L.) Moench) and gamal (Gliricidia sepium).  (Quality evaluation, fermentation characteristic and in vitro digestibility of stay green sorghum-Gliricidia sepium mix silage on different level of lactic acid utilization) ABSTRACT. The grass-legume combined silage is an innovation that can be applied to provide feed and can be used throughout the year. The content of the mixed silage protein can reach optimal levels as a green concentrate feed. This study aims to evaluate the quality, fermentation characteristics and digestibility of in vitro silage of a mixture of sorghum (Sorghum bicolor (L.) Moench) and gamal (Gliricidia sepium) by adding different doses of lactic acid. Silage is made by mixing a constituent consisting of sorghum and gamal leaves in a ratio of 1: 1 and the addition of lactic acid concentration of 20% (v / v). The study consisted of 3 treatments namely; A0: (control) without the addition of lactic acid, A1: addition of 2% (w / v) lactic acid, A2: 2.5% (w / v), and A3: 3% (w / v) with 4 replications each treatment. The ensilage process used goat's rumen fluid for 21 days. The research parameters observed were silage quality and in vitro digestibility. The results showed that the addition dose of lactic acid as much as 2% produced the most optimal silage with pH (3.94), dry matter (26.62%), and Fleigh value (100.89), but had no effect on crude protein content, digestibility organic matter, digestibility of dry matter and digestibility of crude protein. The conclusion of this study is the use of organic lactic acid with a dose of 2 to 3% (w/v) concentration of 20% (v/v) can improve the characteristics, quality of fermentation but does not affect the value of KcBK, KcBO and KcPK silage stay sorghum mixture green (Sorghum bicolor (L.) Moench) and gamal (Gliricidia sepium).

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