scholarly journals Intake of Lactobacillus delbrueckii (pExu:hsp65) Prevents the Inflammation and the Disorganization of the Intestinal Mucosa in a Mouse Model of Mucositis

2021 ◽  
Vol 9 (1) ◽  
pp. 107
Author(s):  
Fernanda Alvarenga Lima Barroso ◽  
Luís Cláudio Lima de Jesus ◽  
Camila Prosperi de Castro ◽  
Viviane Lima Batista ◽  
Ênio Ferreira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Small Bowel ◽  
Mouse Model ◽  
Intestinal Permeability ◽  
Immune Modulation ◽  
Inflammatory Diseases ◽  
Probiotic Strain ◽  
Lactobacillus Delbrueckii ◽  
Preventive Strategy ◽  
Inflammatory Conditions

5-Fluorouracil (5-FU) is an antineoplastic drug that causes, as a side effect, intestinal mucositis, acute inflammation in the small bowel. The Heat Shock Protein (Hsp) are highly expressed in inflammatory conditions, developing an important role in immune modulation. Thus, they are potential candidates for the treatment of inflammatory diseases. In the mucositis mouse model, the present study aimed to evaluate the beneficial effect of oral administration of milk fermented by Lactobacillus delbrueckii CIDCA 133 (pExu:hsp65), a recombinant strain. This approach showed increased levels of sIgA in the intestinal fluid, reducing inflammatory infiltrate and intestinal permeability. Additionally, the histological score was improved. Protection was associated with a reduction in the gene expression of pro-inflammatory cytokines such as Tnf, Il6, Il12, and Il1b, and an increase in Il10, Muc2, and claudin 1 (Cldn1) and 2 (Cldn2) gene expression in ileum tissue. These findings are corroborated with the increased number of goblet cells, the electronic microscopy images, and the reduction of intestinal permeability. The administration of milk fermented by this recombinant probiotic strain was also able to reverse the high levels of gene expression of Tlrs caused by the 5-FU. Thus, the rCIDCA 133:Hsp65 strain was revealed to be a promising preventive strategy for small bowel inflammation.

Immune modulation property of Lactobacillus paracasei NCC2461 (ST11) strain and impact on skin defences

2014 ◽  
Vol 5 (2) ◽  
pp. 129-136 ◽  
Author(s):  
J. Benyacoub ◽  
N. Bosco ◽  
C. Blanchard ◽  
A. Demont ◽  
D. Philippe ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Modulation ◽  
Inflammatory Diseases ◽  
Skin Barrier ◽  
Probiotic Strain ◽  
Skin Barrier Function ◽  
Immune Functions ◽  
Skin Immune System ◽  
Skin Physiology ◽  
Immune Dysfunctions

The gut intestinal tract harbours a complex microbiota. Disturbances in the microbiota composition have been associated with several immune dysfunctions such as inflammatory diseases. Specific strains of probiotics have shown to beneficially influence the composition and/or metabolic activity of the endogenous microbiota. Taking advantage of the plasticity of the immune system, the probiotic strain NCC2461 (i.e. ST11 or CNCM I-2116) supports and/or restores homeostasis in reaction to different physiopathological conditions. The potential of NCC2461 to modulate both mucosal and systemic immune functions led us to test its impact on skin physiology. Even though clear mechanisms explaining gut-skin interaction are still lacking, a set of experimental and clinical data reviewed herein have shown that NCC2461 exerts its effects beyond the gut and confers benefits at the skin level. It contributes to the reinforcement of skin barrier function, decreases skin sensitivity and modulates the skin immune system leading to the preservation of skin homeostasis.

scholarly journals Comparative Transcriptome Analysis of the Expression of Antioxidant and Immunity Genes in the Spleen of a Cyanidin 3-O-Glucoside-Treated Alzheimer’s Mouse Model

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1435
Author(s):  
Varun Jaiswal ◽  
Miey Park ◽  
Hae-Jeung Lee
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Transcriptome Analysis ◽  
Immune Modulation ◽  
Expression Profiles ◽  
Critical Role ◽  
Therapeutic Effects ◽  
Comparative Transcriptome ◽  
Antioxidant Genes ◽  
Comparative Transcriptome Analysis

Cyanidin 3-O-glucoside (C3G) is a well-known antioxidant found as a dietary anthocyanin in different fruits and vegetables. It has protective and therapeutic effects on various diseases. It can reduce neuronal death from amyloid-beta (Aβ)-induced toxicity and promote the inhibition of Aβ fibrillization. Antioxidant and immune modulation might play a critical role in the properties of C3G against Alzheimer’s disease (AD) and other diseases. However, limited studies have been performed on the mechanism involved in the effect of C3G through transcriptome analysis. Thus, the objective of this study was to perform comparative transcriptome analysis of the spleen to determine gene expression profiles of wild-type mice (C57BL/6J Jms), an Alzheimer’s mouse model (APPswe/PS1dE9 mice), and a C3G-treated Alzheimer’s mouse model. Differentially expressed antioxidant, immune-related, and AD pathways genes were identified in the treated group. The validation of gene expression data via RT-PCR studies further supported the current findings. Six important antioxidant genes (S100a8, S100a9, Prdx2, Hp, Mpst, and Prxl2a) and a high number of immune-related genes were found to be upregulated in the treatment groups, suggesting the possible antioxidant and immunomodulatory mechanisms of C3G, respectively. Further studies are strongly recommended to elucidate the precise role of these essential genes and optimize the therapeutic function of C3G in AD and other disease conditions.

scholarly journals Probiogenomics of Lactobacillus delbrueckii subsp. lactis CIDCA 133: In Silico, In Vitro, and In Vivo Approaches

2021 ◽  
Vol 9 (4) ◽  
pp. 829
Author(s):  
Luís Cláudio Lima de Jesus ◽  
Mariana Martins Drumond ◽  
Flávia Figueira Aburjaile ◽  
Thiago de Jesus Sousa ◽  
Nina Dias Coelho-Rocha ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Silico ◽  
Genomic Analysis ◽  
Probiotic Strain ◽  
Lactobacillus Delbrueckii ◽  
Cytoplasmic Proteins ◽  
Genomic Approach ◽  
Lactobacillus Delbrueckii Subsp

Lactobacillus delbrueckii subsp. lactis CIDCA 133 (CIDCA 133) has been reported as a potential probiotic strain, presenting immunomodulatory properties. This study investigated the possible genes and molecular mechanism involved with a probiotic profile of CIDCA 133 through a genomic approach associated with in vitro and in vivo analysis. Genomic analysis corroborates the species identification carried out by the classical microbiological method. Phenotypic assays demonstrated that the CIDCA 133 strain could survive acidic, osmotic, and thermic stresses. In addition, this strain shows antibacterial activity against Salmonella Typhimurium and presents immunostimulatory properties capable of upregulating anti-inflammatory cytokines Il10 and Tgfb1 gene expression through inhibition of Nfkb1 gene expression. These reported effects can be associated with secreted, membrane/exposed to the surface and cytoplasmic proteins, and bacteriocins-encoding genes predicted in silico. Furthermore, our results showed the genes and the possible mechanisms used by CIDCA 133 to produce their beneficial host effects and highlight its use as a probiotic microorganism.

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