scholarly journals Interaction of Styrylpyridinium Compound with Pathogenic Candida albicans Yeasts and Human Embryonic Kidney HEK-293 Cells

2020 ◽  
Vol 9 (1) ◽  
pp. 48
Author(s):  
Simona Vaitkienė ◽  
Laura Bekere ◽  
Gunars Duburs ◽  
Rimantas Daugelavičius
Keyword(s):  
Candida Albicans ◽  
Mammalian Cells ◽  
Efflux Pump ◽  
Efflux Transporter ◽  
Human Embryonic Kidney ◽  
Abcb1 Gene ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Lipophilic Cation

Candida albicans-caused local and systemic diseases are a serious health issue worldwide, leading to high mycosis-associated morbidity and mortality. Efficient combinations of novel compounds with commonly used antifungals could be an important tool for fighting infections. The aim of this study was to evaluate the interaction of synthesized 4-(4-cyanostyryl)-1-dodecylpyridin-1-ium (CSDP+) bromide alone or in combination with fluconazole with yeast and mammalian cells. We investigated cytotoxicity of the tested agents to mammalian HEK-293 cells and the influence of CSDP+ on the ability of C. albicans wt and a clinical isolate to adhere to HEK-293. Accumulation of lipophilic cation ethidium (Et+) was used to monitor the activity of efflux pumps in HEK-293 cells. The effect of CSDP+ on the expression of the main efflux transporter genes and transcription factors in C.albicans cells as well as HEK-293 efflux pump gene ABCB1 was determined. The study showed that CSDP+ alone and in combination with fluconazole was nontoxic to HEK-293 cells and was able to reduce C.albicans adhesion. The treatment of C.albicans cells with CSDP+ in combination with fluconazole resulted in a considerable overexpression of the MDR1 and MRR1 genes. The findings suggest that these genes could be associated with efflux-related resistance to fluconazole. Measurements of Et+ fluorescence and analysis of ABCB1 gene expression demonstrated that mammalian cells were not sensitive to concentrations of CSDP+ affecting C. albicans.

scholarly journals Depletion of the polyamines spermidine and spermine by overexpression of spermidine/spermine N1-acetyltransferase 1 (SAT1) leads to mitochondria-mediated apoptosis in mammalian cells

2015 ◽  
Vol 468 (3) ◽  
pp. 435-447 ◽  
Author(s):  
Swati Mandal ◽  
Ajeet Mandal ◽  
Myung Hee Park
Keyword(s):  
Mammalian Cells ◽  
Growth Arrest ◽  
Mitochondrial Pathway ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Catabolic Enzyme

Adenoviral overexpression of a polyamine catabolic enzyme spermidine (SSAT1)/spermine N1-acetyltransferase 1 (SAT1) in human embryonic kidney (HEK) 293 cells leads to a rapid depletion of spermidine and spermine, growth arrest and apoptosis through the intrinsic mitochondrial pathway.

Effect of Curcumin on Cisplatin- and Oxaliplatin-Induced Oxidative Stress in Human Embryonic Kidney (HEK) 293 Cells

Renal Failure ◽  
2011 ◽  
Vol 33 (5) ◽  
pp. 518-523 ◽  
Author(s):  
Mostafa I. Waly ◽  
Mansour S. Al Moundhri ◽  
Badreldin H. Ali
Keyword(s):  
Oxidative Stress ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

Endogenous expression of the atypical chemokine receptor CCX-CKR (CCRL1) gene in human embryonic kidney (HEK 293) cells

2015 ◽  
Vol 412 (1-2) ◽  
pp. 229-233 ◽  
Author(s):  
Parvin Salimi ◽  
Abolghasem Esmaeili ◽  
Mohammad Hashemi ◽  
Mohaddeseh Behjati
Keyword(s):  
Chemokine Receptor ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
Endogenous Expression ◽  
293 Cells

scholarly journals Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts

2016 ◽  
Vol 94 ◽  
pp. 195-207 ◽  
Author(s):  
Audrey Desvergne ◽  
Nicolas Ugarte ◽  
Sabrina Radjei ◽  
Monique Gareil ◽  
Isabelle Petropoulos ◽  
...  
Keyword(s):  
Dermal Fibroblasts ◽  
Proteasome Activity ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

scholarly journals Human geneSLC41A1encodes for the Na+/Mg2+exchanger

2012 ◽  
Vol 302 (1) ◽  
pp. C318-C326 ◽  
Author(s):  
Martin Kolisek ◽  
Axel Nestler ◽  
Jürgen Vormann ◽  
Monika Schweigel-Röntgen
Keyword(s):  
Mammalian Cells ◽  
Molecular Level ◽  
Transport Activity ◽  
Human Embryonic Kidney ◽  
Efflux System ◽  
Dependent Protein Kinase ◽  
Complete Replacement ◽  
Hek 293 ◽  
293 Cells ◽  
Dependent Protein

Magnesium (Mg2+), the second most abundant divalent intracellular cation, is involved in the vast majority of intracellular processes, including the synthesis of nucleic acids, proteins, and energy metabolism. The concentration of intracellular free Mg2+([Mg2+]i) in mammalian cells is therefore tightly regulated to its optimum, mainly by an exchange of intracellular Mg2+for extracellular Na+. Despite the importance of this process for cellular Mg2+homeostasis, the gene(s) encoding for the functional Na+/Mg2+exchanger is (are) still unknown. Here, using the fluorescent probe mag-fura 2 to measure [Mg2+]ichanges, we examine Mg2+extrusion from hSLC41A1-overexpressing human embryonic kidney (HEK)-293 cells. A three- to fourfold elevation of [Mg2+]iwas accompanied by a five- to ninefold increase of Mg2+efflux. The latter was strictly dependent on extracellular Na+and reduced by 91% after complete replacement of Na+with N-methyl-d-glucamine. Imipramine and quinidine, known unspecific Na+/Mg2+exchanger inhibitors, led to a strong 88% to 100% inhibition of hSLC41A1-related Mg2+extrusion. In addition, our data show regulation of the transport activity via phosphorylation by cAMP-dependent protein kinase A. As these are the typical characteristics of a Na+/Mg2+exchanger, we conclude that the human SLC41A1 gene encodes for the Na+/Mg2+exchanger, the predominant Mg2+efflux system. Based on this finding, the analysis of Na+/Mg2+exchanger regulation and its involvement in the pathogenesis of diseases such as Parkinson's disease and hypertension at the molecular level should now be possible.

scholarly journals The use of human embryonic kidney (HEK 293) cells to enhance characterization of the LMX1B pathway

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Robert Patrick Stump ◽  
Jennifer M Feenstra ◽  
Michael A Castillo ◽  
Salvador Soriano ◽  
Kerby C Oberg
Keyword(s):  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

Transient expression of recombinant ACKR4 (CCRL1) gene, an atypical chemokine receptor in human embryonic kidney (HEK 293) cells

2016 ◽  
Vol 43 (7) ◽  
pp. 583-589 ◽  
Author(s):  
Bahareh Parsi ◽  
Abolghasem Esmaeili ◽  
Mohammad Hashemi ◽  
Mohaddeseh Behjati
Keyword(s):  
Transient Expression ◽  
Chemokine Receptor ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells
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