scholarly journals A Gnotobiotic Model to Examine Plant and Microbiome Contributions to Survival under Arsenic Stress

2020 ◽  
Vol 9 (1) ◽  
pp. 45
Author(s):  
María del Carmen Molina ◽  
James F. White ◽  
Sara García-Salgado ◽  
M. Ángeles Quijano ◽  
Natalia González-Benítez
Keyword(s):  
Seedling Growth ◽  
Endophytic Bacteria ◽  
Root Hairs ◽  
Resistant Bacteria ◽  
Relative Importance ◽  
Early Stages ◽  
Bacterial Interaction ◽  
Arsenic Stress ◽  
Free Media

So far, the relative importance of the plant and its microbiome in the development of early stages of plant seedling growth under arsenic stress has not been studied. To test the role of endophytic bacteria in increasing plant success under arsenic stress, gnotobiotic seeds of J. montana were inoculated with two endophytic bacteria: Pantoea conspicua MC-K1 (PGPB and As resistant bacteria) and Arthrobacter sp. MC-D3A (non-helper and non-As resistant bacteria) and an endobacteria mixture. In holobiotic seedlings (with seed-vectored microbes intact), neither the capacity of germination nor development of roots and lateral hairs was affected at 125 μM As(V). However, in gnotobiotic seedlings, the plants are negatively impacted by absence of a microbiome and presence of arsenic, resulting in reduced growth of roots and root hairs. The inoculation of a single PGPB (P. conspicua-MCK1) shows a tendency to the recovery of the plant, both in arsenic enriched and arsenic-free media, while the inoculation with Arthrobacter sp. does not help in the recovery of the plants. Inoculation with a bacterial mixture allows recovery of plants in arsenic free media; however, plants did not recover under arsenic stress, probably because of a bacterial interaction in the mixture.

Population Control (Review Article)

1961 ◽  
Vol 1 (3) ◽  
pp. 89-98
Author(s):  
Karol J. Krotki
Keyword(s):  
Population Control ◽  
Review Article ◽  
Small Enterprise ◽  
Opportunity Costs ◽  
Relative Importance ◽  
Factual Information ◽  
Undue Influence ◽  
Clear Cut ◽  
Economic Considerations

Discussions about the role of small enterprise in economic development tend to remain inconclusive partly because of the difficulty of assessing the relative importance of economic and non-economic objectives and partly because of the dearth of factual information on which to base an economic calculus. It is probably true, moreover, that, because of a lack of general agreement as to the economic case for or against small enterprise, non-economic considerations, including some merely romantic attitudes toward smallness and bigness, tend to exert an undue influence on public policies. There may, of course, be no clear-cut economic case. And noneconomic considerations should and will inevitably weigh significantly in policy decisions. If, however, some of the economic questions could be settled by more and better knowledge, these decisions could more accurately reflect the opportunity costs of pursuing non-economic objectives.

Injecting Knowledge

2017 ◽  
Author(s):  
Lucia Dacome
Keyword(s):  
Eighteenth Century ◽  
Blood Vessels ◽  
Comparative Perspective ◽  
Anatomical Models ◽  
Early Stages ◽  
The Creation ◽  
Cabinet Of Curiosities

Chapter 7 furthers the analysis of the role of anatomical models as cultural currencies capable of transferring value. It does so by expanding the investigation of the early stages of anatomical modelling to include a new setting. In particular, it follows the journey of the Palermitan anatomist and modeller Giuseppe Salerno and his anatomical ‘skeleton’—a specimen that represented the body’s complex web of blood vessels and was presented as the result of anatomical injections. Although Salerno was headed towards Bologna, a major centre of anatomical modelling, he ended his journey in Naples after the nobleman Raimondo di Sangro purchased the skeleton for his own cabinet of curiosities. This chapter considers the creation and viewing of an anatomical display in di Sangro’s Neapolitan Palace from a comparative perspective that highlights how geography and locality played an important part in shaping the culture of mid-eighteenth-century anatomical modelling.

scholarly journals Role of propagule pressure in colonization success: disentangling the relative importance of demographic, genetic and habitat effects

2013 ◽  
Vol 26 (8) ◽  
pp. 1691-1699 ◽  
Author(s):  
R. A. Hufbauer ◽  
A. Rutschmann ◽  
B. Serrate ◽  
H. Vermeil de Conchard ◽  
B. Facon
Keyword(s):  
Propagule Pressure ◽  
Relative Importance ◽  
Colonization Success

scholarly journals The role of endophytic bacteria and mycorrhizae fungus as plant growth inducer of white turmeric

2021 ◽  
Vol 759 (1) ◽  
pp. 012025
Author(s):  
R Simarmata ◽  
Nuriyanah ◽  
L Nurjanah ◽  
J R L Sylvia ◽  
T Widowati
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria

scholarly journals 3,4-dehydro-L-proline Induces Programmed Cell Death in the Roots of Brachypodium distachyon

2021 ◽  
Vol 22 (14) ◽  
pp. 7548
Author(s):  
Artur Pinski ◽  
Alexander Betekhtin ◽  
Jolanta Kwasniewska ◽  
Lukasz Chajec ◽  
Elzbieta Wolny ◽  
...  
Keyword(s):  
Cell Death ◽  
Brachypodium Distachyon ◽  
Root Hairs ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Cell Wall Proteins ◽  
Root Epidermis ◽  
Root Hair Development ◽  
Transmission Electron ◽  
Hair Development

As cell wall proteins, the hydroxyproline-rich glycoproteins (HRGPs) take part in plant growth and various developmental processes. To fulfil their functions, HRGPs, extensins (EXTs) in particular, undergo the hydroxylation of proline by the prolyl-4-hydroxylases. The activity of these enzymes can be inhibited with 3,4-dehydro-L-proline (3,4-DHP), which enables its application to reveal the functions of the HRGPs. Thus, to study the involvement of HRGPs in the development of root hairs and roots, we treated seedlings of Brachypodium distachyon with 250 µM, 500 µM, and 750 µM of 3,4-DHP. The histological observations showed that the root epidermis cells and the cortex cells beneath them ruptured. The immunostaining experiments using the JIM20 antibody, which recognizes the EXT epitopes, demonstrated the higher abundance of this epitope in the control compared to the treated samples. The transmission electron microscopy analyses revealed morphological and ultrastructural features that are typical for the vacuolar-type of cell death. Using the TUNEL test (terminal deoxynucleotidyl transferase dUTP nick end labelling), we showed an increase in the number of nuclei with damaged DNA in the roots that had been treated with 3,4-DHP compared to the control. Finally, an analysis of two metacaspases’ gene activity revealed an increase in their expression in the treated roots. Altogether, our results show that inhibiting the prolyl-4-hydroxylases with 3,4-DHP results in a vacuolar-type of cell death in roots, thereby highlighting the important role of HRGPs in root hair development and root growth.

scholarly journals The Arctic as a “Strategic” and “Important” Chinese Foreign Policy Interest: Exploring the Role of Labels and Hierarchies in China’s Arctic Discourses

2021 ◽  
pp. 186810262110186
Author(s):  
Patrik Andersson
Keyword(s):  
Foreign Policy ◽  
International Relations ◽  
Political Discourse ◽  
Chinese Foreign Policy ◽  
The Arctic ◽  
Relative Importance ◽  
Precise Understanding ◽  
New Frontier ◽  
Political Priority

Research confirms that China is becoming more engaged in the Arctic. However, international relations scholarship often extrapolates from relatively few instances of activity to wide-ranging claims about Chinese priorities. Fortunately, Chinese political discourse is organised by labels that allow us to study how the Arctic is classified and ranked along China’s other foreign policy priorities. This article analyses two such classifications – “important maritime interest” and “strategic new frontier,” exploring how they have come about, what they mean, and how they add political priority to the Arctic. It argues that hierarchies are constructed in two ways: by adding gradients and by including/excluding categories of priority. It views categories as performative: they not only convey information about character and relative importance of interests but are also used for achieving different objectives. By focusing on foreign policy classifications, the article contributes to a more nuanced and precise understanding of China’s Arctic interests.

Limited Influence? The Role of the Party of European Socialists in Shaping Social Democracy in Central and Eastern Europe

2011 ◽  
Vol 46 (1) ◽  
pp. 32-55 ◽  
Author(s):  
Michael Holmes ◽  
Simon Lightfoot
Keyword(s):  
Eastern Europe ◽  
Central And Eastern Europe ◽  
Social Democracy ◽  
Social Democratic ◽  
Academic Literature ◽  
Early Stages ◽  
European Norm ◽  
Democratic Parties ◽  
Social Democratic Parties

AbstractThis article looks at the role of the Party of European Socialists (PES) in its attempts to shape social democratic parties in Central and Eastern Europe (CEE) towards a West European norm. It discusses how existing views in the academic literature on the role of transnational parties are inadequate. We argue that the PES did not play a key role in encouraging the establishment and development of parties in the CEE states from the 2004 enlargement in the early stages of accession. We contend that the overall influence of party federations has been limited, and that these limitations were as much in evidence before enlargement took place as they were afterwards.

Study of the role of Chlamydia, Mycoplasma, Ureaplasma and other microaerophilic and aerobic bacteria in uterine infections of mares with reproductive disorders

2003 ◽  
Vol 51 (1) ◽  
pp. 45-52 ◽  
Author(s):  
László Szeredi ◽  
M. Tenk ◽  
I. Schiller ◽  
Keyword(s):  
Pathogenic Bacteria ◽  
Anaerobic Bacteria ◽  
Aerobic Bacteria ◽  
Relative Importance ◽  
Reproductive Disorders ◽  
Taylorella Equigenitalis

In six healthy mares and 24 mares showing reproductive disorders swab samples were taken from the fossa clitoridis to isolate Taylorella equigenitalis, and from the uterus to isolate mycoplasmas, ureaplasmas and other aerobic bacteria. Swab samples were also taken from the uterus for Chlamydiaantigen ELISA and ChlamydiaPCR studies. The uterus of 27 mares was examined cytologically, and biopsy samples were taken from the endometrium for histological examinations and for immunohistochemical examinations aimed at the detection of chlamydiae. T. equigenitalis, mycoplasmas, ureaplasmas and chlamydiae could not be detected from any of the mares examined. Aerobic facultative pathogenic bacteria were isolated from mares with endometritis in four cases. In 18 out of 22 mares with endometritis (82%) no infective agents could be demonstrated. Further studies are needed to elucidate the relative importance of non-infectious causes of endometritis and of anaerobic bacteria often detectable in the uterus in the aetiology of the reproductive disorders observed.

The role of digital artefacts in early stages of distributed innovation processes

2021 ◽  
Vol 50 (10) ◽  
pp. 104349
Author(s):  
Markus C. Becker ◽  
Francesco Rullani ◽  
Francesco Zirpoli
Keyword(s):  
Early Stages ◽  
Innovation Processes
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