scholarly journals Outer Membrane Vesicles Derived from Klebsiella pneumoniae Influence the miRNA Expression Profile in Human Bronchial Epithelial BEAS-2B Cells

2020 ◽  
Vol 8 (12) ◽  
pp. 1985
Author(s):  
Federica Dell’Annunziata ◽  
Concetta Paola Ilisso ◽  
Carmela Dell’Aversana ◽  
Giuseppe Greco ◽  
Alessandra Coppola ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Klebsiella Pneumoniae ◽  
Outer Membrane ◽  
Expression Profile ◽  
Mirna Expression ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

Klebsiella pneumoniae is an opportunistic pathogen that causes nosocomial and community-acquired infections. The spread of resistant strains of K. pneumoniae represents a growing threat to human health, due to the exhaustion of effective treatments. K. pneumoniae releases outer membrane vesicles (OMVs). OMVs are a vehicle for the transport of virulence factors to host cells, causing cell injury. Previous studies have shown changes of gene expression in human bronchial epithelial cells after treatment with K. pneumoniae OMVs. These variations in gene expression could be regulated through microRNAs (miRNAs), which participate in several biological mechanisms. Thereafter, miRNA expression profiles in human bronchial epithelial cells were evaluated during infection with standard and clinical K. pneumoniae strains. Microarray analysis and RT-qPCR identified the dysregulation of miR-223, hsa-miR-21, hsa-miR-25 and hsa-let-7g miRNA sequences. Target gene prediction revealed the essential role of these miRNAs in the regulation of host immune responses involving NF-ĸB (miR-223), TLR4 (hsa-miR-21), cytokine (hsa-miR-25) and IL-6 (hsa-let-7g miRNA) signalling pathways. The current study provides the first large scale expression profile of miRNAs from lung cells and predicted gene targets, following exposure to K. pneumoniae OMVs. Our results suggest the importance of OMVs in the inflammatory response.

GENE EXPRESSION PROFILE BY RNAi-MEDIATED SUPPRESSION OF S100A2 IN HUMAN BRONCHIAL EPITHELIAL CELLS

Toukeibu Gan ◽  
2005 ◽  
Vol 31 (4) ◽  
pp. 481-486
Author(s):  
Nobuhiko ORIDATE ◽  
Takatsugu MIZUMACHI ◽  
Fumiyuki SUZUKI ◽  
Akihiro HOMMA ◽  
Yasushi FURUTA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

scholarly journals Gene Expression Profile and Toxic Effects in Human Bronchial Epithelial Cells Exposed to Zearalenone

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96404 ◽  
Author(s):  
Mei Yu So ◽  
ZhiPeng Tian ◽  
Yong Shian Phoon ◽  
Sha Sha ◽  
Michael N. Antoniou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Bronchial Epithelial Cells ◽  
Toxic Effects ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

scholarly journals Lipidomic Analysis of the Outer Membrane Vesicles from Paired Polymyxin-Susceptible and -Resistant Klebsiella pneumoniae Clinical Isolates

2018 ◽  
Vol 19 (8) ◽  
pp. 2356 ◽  
Author(s):  
Raad Jasim ◽  
Mei-Ling Han ◽  
Yan Zhu ◽  
Xiaohan Hu ◽  
Maytham Hussein ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Average Diameter ◽  
Polymyxin B ◽  
Outer Membrane Vesicles ◽  
Resistant Isolate ◽  
Average Particle ◽  
Lipid Species ◽  
The Impact

Gram-negative bacteria produce outer membrane vesicles (OMVs) as delivery vehicles for nefarious bacterial cargo such as virulence factors, which are antibiotic resistance determinants. This study aimed to investigate the impact of polymyxin B treatment on the OMV lipidome from paired polymyxin-susceptible and -resistant Klebsiella pneumoniae isolates. K. pneumoniae ATCC 700721 was employed as a reference strain in addition to two clinical strains, K. pneumoniae FADDI-KP069 and K. pneumoniae BM3. Polymyxin B treatment of the polymyxin-susceptible strains resulted in a marked reduction in the glycerophospholipid, fatty acid, lysoglycerophosphate and sphingolipid content of their OMVs. Conversely, the polymyxin-resistant strains expressed OMVs richer in all of these lipid species, both intrinsically and increasingly under polymyxin treatment. The average diameter of the OMVs derived from the K. pneumoniae ATCC 700721 polymyxin-susceptible isolate, measured by dynamic light scattering measurements, was ~90.6 nm, whereas the average diameter of the OMVs isolated from the paired polymyxin-resistant isolate was ~141 nm. Polymyxin B treatment (2 mg/L) of the K. pneumoniae ATCC 700721 cells resulted in the production of OMVs with a larger average particle size in both the susceptible (average diameter ~124 nm) and resistant (average diameter ~154 nm) strains. In light of the above, we hypothesize that outer membrane remodelling associated with polymyxin resistance in K. pneumoniae may involve fortifying the membrane structure with increased glycerophospholipids, fatty acids, lysoglycerophosphates and sphingolipids. Putatively, these changes serve to make the outer membrane and OMVs more impervious to polymyxin attack.

Microarray-assisted size-effect study of amorphous silica nanoparticles on human bronchial epithelial cells

Nanoscale ◽  
2019 ◽  
Vol 11 (47) ◽  
pp. 22907-22923 ◽  
Author(s):  
Yang Li ◽  
Junchao Duan ◽  
Xiangyuan Chai ◽  
Man Yang ◽  
Ji Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Size Effect ◽  
Toxic Effect ◽  
Silica Nanoparticles ◽  
Amorphous Silica ◽  
Effect Study ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Size Dependent

Silica nanoparticles produced size-dependent toxic effect on the gene expression profile of BEAS-2B cells.

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