scholarly journals Bioreactivity, Guttation and Agents Influencing Surface Tension of Water Emitted by Actively Growing Indoor Mould Isolates

2020 ◽  
Vol 8 (12) ◽  
pp. 1940
Author(s):  
Maria A. Andersson ◽  
Johanna Salo ◽  
Orsolya Kedves ◽  
László Kredics ◽  
Irina Druzhinina ◽  
...  
Keyword(s):  
Indoor Air ◽  
Fungal Growth ◽  
Penicillium Expansum ◽  
Aspergillus Versicolor ◽  
Liquid Droplets ◽  
Mammalian Cell Lines ◽  
Surfactant Activity ◽  
Wet Weight ◽  
Rhizopus Sp ◽  
Indoor Mould

The secretion of metabolites in guttation droplets by indoor moulds is not well documented. This study demonstrates the guttation of metabolites by actively growing common indoor moulds. Old and fresh biomasses of indoor isolates of Aspergillus versicolor, Chaetomium globosum, Penicillium expansum, Trichoderma atroviride, T. trixiae, Rhizopus sp. and Stachybotrys sp. were compared. Metabolic activity indicated by viability staining and guttation of liquid droplets detected in young (<3 weeks old) biomass were absent in old (>6 months old) cultures consisting of dehydrated hyphae and dormant conidia. Fresh (<3 weeks old) biomasses were toxic more than 10 times towards mammalian cell lines (PK-15 and MNA) compared to the old dormant, dry biomasses, when calculated per biomass wet weight and per conidial particle. Surfactant activity was emitted in exudates from fresh biomass of T. atroviride, Rhizopus sp. and Stachybotrys sp. Surfactant activity was also provoked by fresh conidia from T. atroviride and Stachybotrys sp. strains. Water repealing substances were emitted by cultures of P. expansum, T. atroviride and C. globosum strains. The metabolic state of the indoor fungal growth may influence emission of liquid soluble bioreactive metabolites into the indoor air.

scholarly journals New Isolated Metschnikowia pulcherrima Strains from Apples for Postharvest Biocontrol of Penicillium expansum and Patulin Accumulation

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 397
Author(s):  
Laura Settier-Ramírez ◽  
Gracia López-Carballo ◽  
Pilar Hernández-Muñoz ◽  
Angélique Fontana ◽  
Caroline Strub ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Fungal Growth ◽  
Its Region ◽  
Antagonistic Activity ◽  
Penicillium Expansum ◽  
Metschnikowia Pulcherrima ◽  
Potential Activity ◽  
Different Strains ◽  
Main Producer

Wild yeasts isolated from the surface of apples were screened for antagonistic activity against Penicillium expansum, the main producer of the mycotoxin patulin. Three antagonistic yeasts (Y33, Y29 and Y24) from a total of 90 were found to inhibit P. expansum growth. Identification by ITS region sequence and characterization showed that three selected isolates of yeast should be different strains of Metschnikowia pulcherrima. Several concentrations of the selected yeasts were used to study their in vitro antifungal effectivity against P. expansum on Petri dishes (plates with 63.6 cm2 surface) whereas their potential activity on patulin reduction was studied in liquid medium. Finally, the BCA that had the best in vitro antifungal capacity against P. and the best patulin degradation capacity was selected to be assessed directly on apples. All the selected strains demonstrated antifungal activity in vitro but the most efficient was the strain Y29. Isolated strains were able to reduce patulin content in liquid medium, Y29 being the only strain that completely reduced patulin levels within 120 h. The application of Y29 as biocontrol agent on the surface of apples inoculated with P. expansum, inhibited fungal growth and patulin production during storage. Therefore, the results shown that this yeast strain could be used for the reduction of P. expansum and its mycotoxin in apples or apple-based products by adapting the procedure application.

scholarly journals Blistering1 Modulates Penicillium expansum Virulence Via Vesicle-mediated Protein Secretion

2019 ◽  
Vol 19 (2) ◽  
pp. 344-361 ◽  
Author(s):  
Wayne M. Jurick ◽  
Hui Peng ◽  
Hunter S. Beard ◽  
Wesley M. Garrett ◽  
Franz J. Lichtner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Secretion ◽  
Cross Sections ◽  
Fungal Growth ◽  
Single Copy ◽  
Protein Processing ◽  
Culture Broth ◽  
Penicillium Expansum ◽  
Mold Fungus ◽  
Host Infection

The blue mold fungus, Penicillium expansum, is a postharvest apple pathogen that contributes to food waste by rotting fruit and by producing harmful mycotoxins (e.g. patulin). To identify genes controlling pathogen virulence, a random T-DNA insertional library was created from wild-type P. expansum strain R19. One transformant, T625, had reduced virulence in apples, blistered mycelial hyphae, and a T-DNA insertion that abolished transcription of the single copy locus in which it was inserted. The gene, Blistering1, encodes a protein with a DnaJ domain, but otherwise has little homology outside the Aspergillaceae, a family of fungi known for producing antibiotics, mycotoxins, and cheese. Because protein secretion is critical for these processes and for host infection, mass spectrometry was used to monitor proteins secreted into liquid media during fungal growth. T625 failed to secrete a set of enzymes that degrade plant cell walls, along with ones that synthesize the three final biosynthetic steps of patulin. Consequently, the culture broth of T625 had significantly reduced capacity to degrade apple tissue and contained 30 times less patulin. Quantitative mass spectrometry of 3,282 mycelial proteins revealed that T625 had altered cellular networks controlling protein processing in the endoplasmic reticulum, protein export, vesicle-mediated transport, and endocytosis. T625 also had reduced proteins controlling mRNA surveillance and RNA processing. Transmission electron microscopy of hyphal cross sections confirmed that T625 formed abnormally enlarged endosomes or vacuoles. These data reveal that Blistering1 affects internal and external protein processing involving vesicle-mediated transport in a family of fungi with medical, commercial, and agricultural importance.

INTERACTIONS BETWEEN MAIZE WEEVIL (COLEOPTERA: CURCULIONIDAE) INFESTATIONS AND INFECTION BY ASPERGILLUS FLAVUS AND OTHER FUNGI IN STORED CORN

1987 ◽  
Vol 22 (2) ◽  
pp. 108-118 ◽  
Author(s):  
D. E. Dix ◽  
J. N. All
Keyword(s):  
Moisture Content ◽  
Aspergillus Flavus ◽  
Fungal Growth ◽  
Sitophilus Zeamais ◽  
High Moisture ◽  
Maize Weevil ◽  
Grain Moisture ◽  
Unidentified Species ◽  
Wet Weight ◽  
Bright Blue

Thirty adult maize weevils, Sitophilus zeamais Motschulsky, were placed in six glass-fronted wooden grain storage bins filled with 7.2 kg of shelled corn at 14% moisture content. An auger plug containing a heavily sporulating culture of Aspergillus flavus Link was added to the center of three infested bins and three noninfested bins. Another three bins of corn were untreated controls. All of the bins were stored for 16 weeks at 24°C. Weevil-infested corn began significantly heating (2°C) within nine weeks and began losing weight within 12 weeks. The distribution of fungal growth, high moisture grain, corn dust caking, and maize weevils coincided, being initially restricted to the lower edges of the containers and gradually migrating upward. An unidentified species of Penicillium (bright blue) was observed sporulating within 11 weeks in weevil-infested bins. The bins containing S. zeamais lost an average of 2% of their initial wet weight, and increased in grain moisture and temperature by 20% and 5°C, respectively. Pencillium (bright blue) comprised &gt; 95% of the inoculum present on kernels, however A. flavus comprised &gt; 90% of the inoculum recovered from surface sterilized maize weevils. Increased mortality of teneral adults occurred in A. flavus-inoculated bins. Corn from weevil-free bins, whether inoculated with A. flavus or not, failed to support fungal growth, heat, or lose weight.

scholarly journals Purification and Characterization of Novel Antifungal Compounds from the Sourdough Lactobacillus plantarum Strain 21B

2000 ◽  
Vol 66 (9) ◽  
pp. 4084-4090 ◽  
Author(s):  
Paola Lavermicocca ◽  
Francesca Valerio ◽  
Antonio Evidente ◽  
Silvia Lazzaroni ◽  
Aldo Corsetti ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Wheat Flour ◽  
Culture Filtrate ◽  
Bacterial Culture ◽  
Fungal Growth ◽  
Lactobacillus Brevis ◽  
Penicillium Expansum ◽  
Antifungal Compounds ◽  
Phenyllactic Acid

ABSTRACT Sourdough lactic acid bacteria were selected for antifungal activity by a conidial germination assay. The 10-fold-concentrated culture filtrate of Lactobacillus plantarum 21B grown in wheat flour hydrolysate almost completely inhibited Eurotium repens IBT18000, Eurotium rubrum FTDC3228,Penicillium corylophilum IBT6978, Penicillium roqueforti IBT18687, Penicillium expansum IDM/FS2,Endomyces fibuliger IBT605 and IDM3812, Aspergillus niger FTDC3227 and IDM1, Aspergillus flavus FTDC3226,Monilia sitophila IDM/FS5, and Fusarium graminearum IDM623. The nonconcentrated culture filtrate ofL. plantarum 21B grown in whole wheat flour hydrolysate had similar inhibitory activity. The activity was fungicidal. Calcium propionate at 3 mg ml−1 was not effective under the same assay conditions, while sodium benzoate caused inhibition similar toL. plantarum 21B. After extraction with ethyl acetate, preparative silica gel thin-layer chromatography, and chromatographic and spectroscopic analyses, novel antifungal compounds such as phenyllactic and 4-hydroxy-phenyllactic acids were identified in the culture filtrate of L. plantarum 21B. Phenyllactic acid was contained at the highest concentration in the bacterial culture filtrate and had the highest activity. It inhibited all the fungi tested at a concentration of 50 mg ml−1 except forP. roqueforti IBT18687 and P. corylophilumIBT6978 (inhibitory concentration, 166 mg ml−1). L. plantarum 20B, which showed high antimold activity, was also selected. Preliminary studies showed that phenyllactic and 4-hydroxy-phenyllactic acids were also contained in the bacterial culture filtrate of strain 20B. Growth of A. niger FTDC3227 occurred after 2 days in breads started with Saccharomyces cerevisiae 141 alone or with S. cerevisiae andLactobacillus brevis 1D, an unselected but acidifying lactic acid bacterium, while the onset of fungal growth was delayed for 7 days in bread started with S. cerevisiae and selectedL. plantarum 21B.

scholarly journals Using Hexanal Vapor to Inhibit Activity of Penicillium expansum in Inoculated Whole Apple Fruit

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 536F-537
Author(s):  
Lihua Fan ◽  
Jun Song ◽  
Randolph Beaudry
Keyword(s):  
Decay Rate ◽  
Fungal Growth ◽  
Apple Fruit ◽  
Penicillium Expansum ◽  
Aroma Volatiles ◽  
Free Air ◽  
Air Control ◽  
And Control ◽  
Cessation Of Treatment ◽  
Treated Apple

Hexanal vapor is a natural, metabolizable fungicide that inhibits fungal activity and enhances the aroma biosynthesis in sliced apple fruit. Whole apple fruit were inoculated at two points per fruit with Penicillium expansum at a concentration of 0.5 × 105 spore/ml and treated with hexanal vapors. Inoculated fruit were exposed to hexanal for 48 hr and kept for another 72 hr in hexanal-free air at 22°C. Treatments included 8.2–12.3 μmol·L–1 (200–300 ppm), 14.5-18.6 μmol·L–1 (350–450 ppm), and 24.8-28.9 μmol·L–1 (600–700 ppm), each with an air control. At a concentration of 200–300 ppm hexanal, there was no fungal growth during treatment, but lesion development was evident on 100% of the treated fruit following cessation of treatment. After 72 hr holding in air, lesion diameter was significantly smaller for treated fruit. When inoculated apple fruit were exposed to 350–450 ppm and 600–700 ppm hexanal vapors, the decay rate was 44.7% and 23.9%, respectively, while the decay rate of inoculated control apple fruit was 100% and 98%, respectively, after 72 hr holding in air. The development of aroma volatiles was investigated for both treated and untreated whole apple fruit. Hexanal was actively converted to aroma volatiles by `Golden Delicious' fruit and there was no detectable hexanal emanations. The amount of hexylacetate, hexylbutanoate, hexylhexanoate, hexylpropionate, butylhexanoate, and hexyl-2-methybutanoate were about 2- to 4-fold higher in treated apple fruit than in untreated apple fruit. `Mutsu' apple fruit were treated with 350–450 ppm hexanal for 48 hr and processed into apple sauce within 4 hr. An informal sensory evaluation for processed `Mutsu' apple revealed no apparent flavor difference between treated and control fruit sauce.

scholarly journals Detection the antifungal effect of zirconium oxide nanoparticles on mold which isolated from domestic's bathroom

2018 ◽  
Vol 29 (1) ◽  
pp. 15
Author(s):  
Dina Yousif Mohamed
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Zirconium Oxide ◽  
Fungal Growth ◽  
Oxide Nanoparticles ◽  
Curvularia Lunata ◽  
Antifungal Effect ◽  
Aspergillus Brasiliensis ◽  
Rhizopus Sp ◽  
Zirconium Oxide Nanoparticles

The aim of this study is to detection the antifungal effect of Zirconium oxide nanoparticles on mold which isolated from domestic's bathroom during April 2017 in Baghdad City. Twenty species were isolated from one hundred samples which were Aspergillus niger, Aspergillus. flavus, , Aspergillus duricaulis, Aspergillus nidulans Aspergillus. parasiticus ,Aspergillus fumigatus, Aspergillus. brasiliensis ,Aspergillus heteromorphus , Curvularia lunata, Penicillium sp., Fusarium oxysporum, , Alternaria alternate, Cladosporium sp. Trichoderma sp., Mucor, Rhizopus sp., Rhodotorula mucilaginosa, , Stachybotrys and yeast. Among the isolated species Aspergillus niger, was the most abundant (14.92%) followed by Aspergillus flavus (10.14%), while less abundant (0.95 & 0.63 %) Cladosporium sp. &, Mucor respectively. The higher demoralization of fungal growth was recorded at 8mg/ml concentration of Zirconium oxide nanoparticles which was (3.8cm) in molds Aspergillus niger, Aspergillus. flavus, , & Aspergillus. brasiliensis

scholarly journals KONDISI KUALITAS UDARA DI DALAM RUANGAN PEMUKIMANAN NON-KUMUH KOTA BANDUNG

2020 ◽  
Vol 3 (3) ◽  
pp. 235-245
Author(s):  
R.A Vesitara Kencanasari ◽  
Usep Surahman ◽  
Asep Yudi Permana ◽  
Hari Din Nugraha
Keyword(s):  
Air Quality ◽  
Indoor Air Quality ◽  
Indoor Air ◽  
Sick Building Syndrome ◽  
Fungal Growth ◽  
Cross Sectional ◽  
Normal Limits ◽  
Sick Building ◽  
Temperature Parameter ◽  
Motorized Vehicles

Abstract: This research was initiated by the factors of poor indoor air quality conditions. So that it can cause Sick Building Syndrome (SBS) or sick building syndrome. The purpose of this research is to measure the picture of air quality conditions in non-slum rooms. The sample in this study was Pasteur Village RW6, Sukajadi District which was selected based on criteria, namely including non-slum areas, as well as high community participation. The research method used was cross-sectional. The results showed an overview of indoor air quality in non-slum dwellings, humidity was above the required standard, thus triggering fungal growth rates that exceeded normal limits. The temperature parameter is in the required category, but based on the findings, some people are uncomfortable with the room temperature in the house. The Formadehilda and VOC parameters are within normal limits, this is indicated because most people do not use chemical products, rarely smoke in the house, and rarely start motorized vehicles in the house.Keywords: Indoor Air Quality, Sick Building Sindrome (SBS) Abstrak: Penelitian ini diawali oleh faktor kondisi kualitas udara di dalam ruangan yang buruk. Sehingga dapat menyebabkan terjadinya Sick Building Syndrome (SBS) atau sindrom bangunan sakit. Tujuan peneltian ini adalah untuk mengukur gambaran kondisi kualitas udara di dalam ruangan non kumuh. Sampel pada penelitian ini adalah Kelurahan Pasteur RW6, Kecamatan Sukajadi yang dipilih berdasarkan kriteria yaitu termasuk kawasan non kumuh, serta partisipasi masyarakat yang tinggi. Metode Penelitian yang digunakan adalah cross-sectional. Hasil penelitian menunjukkan gambaran kualitas udara di dalam ruangan pada rumah tinggal non kumuh, kelembaban berada di atas standar yang dipersyaratkan, sehingga memicu tingkat pertumbuhan jamur yang melampaui batas normal. Parameter suhu berada dalam kategori yang dipersyaratkan, namun berdasarkan hasil temuan sebagian masyarakat kurang nyaman dengan suhu ruang pada rumah tinggal. Parameter Formadehilda dan VOC berada pada batas normal, hal tersebut diindikasikan karena sebagian besar masyarakat tidak menggunakan produk-produk kimia, jarang melakukan aktivitas merokok di dalam rumah, serta jarang menghidupkan mesin kendaraan bermotor di dalam rumah. Kata Kunci: kualitas udara di dalam ruangan, Sick Building Sindrome (SBS)

scholarly journals Biological susceptibility of earth plasters: The influence of relative humidity on fungal growth

2019 ◽  
Vol 10 (1) ◽  
pp. 9-14
Author(s):  
Marie Sokolová ◽  
Pavla Ryparová
Keyword(s):  
Relative Humidity ◽  
Indoor Air ◽  
Building Materials ◽  
Construction Materials ◽  
Fungal Growth ◽  
The Earth ◽  
Earthen Construction ◽  
External Conditions ◽  
Microbiological Agents ◽  
Number Of Authors

All building materials can be affected by microbiological agents during their lifecycle. The presence of microorganisms changes the appearance of the surface, degrading it, and they can even cause health problems to the residents. The biological susceptibility is dependent on the content of nutrient based on organic compounds. Thus one of the most susceptible of those materials are earthen construction materials. The degree of fungal growth is influenced by the chemical composition and plant fibres additives as well as the external conditions such as temperature and relative humidity. The earth plastering mortar has started to gain more attention recently as it is considered to have a low environmental impact and to increase the indoor air quality. Mechanical and physical characteristics of earth materials were studied by a number of authors but the knowledge about the biological resistance of the material is scarce. This study intends to look into the issue of the biological colonisation of earth plasters depending on the relative humidity. The samples, made of four types of earth plasters with different plant fibres, were placed to an environment of the relative humidity ranging from 33% to 100%. During a period of 4 weeks the extent of fungal growth was observed.

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