scholarly journals Candida Species (Volatile) Metabotyping through Advanced Comprehensive Two‐Dimensional Gas Chromatography

2020 ◽  
Vol 8 (12) ◽  
pp. 1911
Author(s):  
Carina Pedrosa Costa ◽  
Ana Rita Bezerra ◽  
Adelaide Almeida ◽  
Sílvia M. Rocha
Keyword(s):  
Gas Chromatography ◽  
Candida Species ◽  
Clinical Diagnostics ◽  
Therapeutic Interventions ◽  
Volatile Fraction ◽  
Sulphur Compounds ◽  
New Approach ◽  
Fatty Acids Metabolism ◽  
Species Distinction ◽  
Comprehensive Mapping

Microbial metabolomics is a challenge strategy that allows a comprehensive analysis of metabolites within a microorganism and may support a new approach in microbial research, including the microbial diagnosis. Thus, the aim of this research was to in-depth explore a metabolomics strategy based on the use of an advanced multidimensional gas chromatography for the comprehensive mapping of cellular metabolites of C. albicans and non-C. albicans (C. glabrata and C. tropicalis) and therefore contributing for the development of a comprehensive platform for fungal detection management and for species distinction in early growth times (6 h). The volatile fraction comprises 126 putatively identified metabolites distributed over several chemical families: acids, alcohols, aldehydes, hydrocarbons, esters, ketones, monoterpenic and sesquiterpenic compounds, norisoprenoids, phenols and sulphur compounds. These metabolites may be related with different metabolic pathways, such as amino acid metabolism and biosynthesis, fatty acids metabolism, aromatic compounds degradation, mono and sesquiterpenoid synthesis and carotenoid cleavage. These results represent an enlargement of ca. 70% of metabolites not previously reported for C. albicans, 91% for C. glabrata and 90% for C. tropicalis. This study represents the most detailed study about Candida species exometabolome, allowing a metabolomic signature of each species, which signifies an improvement towards the construction of a Candida metabolomics platform whose application in clinical diagnostics can be crucial to guide therapeutic interventions.

scholarly journals Authentication of the Botanical and Geographical Origin and Detection of Adulteration of Olive Oil Using Gas Chromatography, Infrared and Raman Spectroscopy Techniques: A Review

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1565
Author(s):  
Eleni Kakouri ◽  
Panagiota-Kyriaki Revelou ◽  
Charalabos Kanakis ◽  
Dimitra Daferera ◽  
Christos S. Pappas ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Raman Spectroscopy ◽  
Olive Oil ◽  
Nutritional Value ◽  
Review Paper ◽  
Geographical Origin ◽  
Analytical Techniques ◽  
Volatile Fraction ◽  
Olive Oils ◽  
Infrared And Raman Spectroscopy

Olive oil is among the most popular supplements of the Mediterranean diet due to its high nutritional value. However, at the same time, because of economical purposes, it is also one of the products most subjected to adulteration. As a result, authenticity is an important issue of concern among authorities. Many analytical techniques, able to detect adulteration of olive oil, to identify its geographical and botanical origin and consequently guarantee its quality and authenticity, have been developed. This review paper discusses the use of infrared and Raman spectroscopy as candidate tools to examine the authenticity of olive oils. It also considers the volatile fraction as a marker to distinguish between different varieties and adulterated olive oils, using SPME combined with gas chromatography technique.

scholarly journals Characterisation of the volatile fraction of aromatic caramel using heart-cutting multidimensional gas chromatography

2015 ◽  
Vol 167 ◽  
pp. 281-289 ◽  
Author(s):  
Laurianne Paravisini ◽  
Aurélie Prot ◽  
Cécile Gouttefangeas ◽  
Cédric Moretton ◽  
Henri Nigay ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Volatile Fraction ◽  
Multidimensional Gas Chromatography

Analysis of Organic Acids in Human Fluids by Capillary Gas Chromatography: A New Approach

2003 ◽  
Vol 3 (4) ◽  
pp. 307-313
Author(s):  
Sanja Grkovic ◽  
Ratko Jankov . ◽  
Milica Cvorkov-Drazi . ◽  
Rajko Nikolic .
Keyword(s):  
Gas Chromatography ◽  
Organic Acids ◽  
Capillary Gas Chromatography ◽  
New Approach ◽  
Human Fluids

scholarly journals Volatile Organic Compound and Fatty Acid Profile of Milk from Cows and Buffaloes Fed Mycorrhizal or Nonmycorrhizal Ensiled Forage

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1616 ◽  
Author(s):  
Alessandro Genovese ◽  
Andrea Marrazzo ◽  
Lucia De Luca ◽  
Raffaele Romano ◽  
Nadia Manzo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Mycorrhizal Fungi ◽  
Solid Phase ◽  
Saturated Fatty Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Fraction ◽  
Forage Crops ◽  
Seed Inoculation

Seed inoculation of forage crops by arbuscular mycorrhizal fungi (AMF) generally results in higher profitability, but also modifies the chemical composition of silage in terms of increased biomass, protein, and dry matter. Raw milk aroma is affected by the type of feed. This work investigated the influence of ensiled forage obtained by seed inoculation with AMF on the volatile fractions and fatty acid composition of milk. Two experiments were carried out: in the first, buffaloes were fed maize silage, and in the second, cows were fed sorghum silage. The volatile fractions of milk were quantified by headspace solid-phase micro-extraction (HS-SPME), combined with gas chromatography–mass spectrometry (GC-MS), and fatty acids by gas chromatography (GC). The ensiled forage obtained with AMF increased saturated fatty acids (SFAs), and decreased monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) in both experiments. The volatile fraction in milk samples obtained from bovines fed mycorrhizal ensiled forage showed an increase of free fatty acids and ketones, responsible for cheesy and fruity odors. Aldehydes, responsible for green and grassy notes, increased only in the milk from buffaloes fed ensiled maize. Our results suggest that inoculation of maize and sorghum seed with AMF, combined with a low rate of fertilizers, leads to ensiled forage that could slightly affect the FA profile and odor quality of milk.

Hold-up time in gas chromatography I. New approach to its estimation

1997 ◽  
Vol 760 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Rosa Lebrón-Aguilar ◽  
Jesús Eduardo Quintanilla-López ◽  
JoséAntonio García-Domínquez
Keyword(s):  
Gas Chromatography ◽  
New Approach
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