scholarly journals Glycerophosphodiester Phosphodiesterase Identified as Non-Reliable Serological Marker for Borrelia miyamotoi Disease

2020 ◽  
Vol 8 (12) ◽  
pp. 1846
Author(s):  
Michael Reiter ◽  
Theresa Stelzer ◽  
Anna M. Schötta ◽  
Mateusz Markowicz ◽  
Michael Leschnik ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Blood Donors ◽  
Diagnostic Marker ◽  
Red Cross ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Borrelia Miyamotoi ◽  
High Concentrations ◽  
Pathogen Diagnosis ◽  
Diagnosis Of Infection ◽  
Glycerophosphodiester Phosphodiesterase

The relapsing fever group Borrelia miyamotoi is an emerging tick-borne pathogen. Diagnosis of infection is currently mainly based on serological methods detecting antibodies against B. miyamotoi glycerophosphodiester phosphodiesterase (GlpQ). Here, we scrutinized the reliability of GlpQ as a diagnostic marker and compared the seroprevalence in different study populations and by applying various immunoblotting methods. Antibodies were detected in the sera of 7/53 hunters and in 1/11 sera of Lyme neuroborreliosis patients. Furthermore, 17/74 sera of persons with high concentrations of anti-Borrelia burgdorferi sensu lato (α-Bbsl) antibodies reacted strongly with B. miyamotoi GlpQ in immunoblots. The B. miyamotoi GlpQ seroprevalence was 7/50 in α-Bbsl negative persons. In healthy blood donors from commercial suppliers and from the Austrian Red Cross, seroprevalences were 5/14 and 10/35, respectively. Strikingly, two B. miyamotoi PCR-positive cases from Austria had negative GlpQ serology, indicating poor sensitivity. Finally, when we analyzed sera of dogs, we found α-B. miyamotoi GlpQ antibody seroprevalence in tick-free dogs (n = 10) and in tick-exposed dogs (n = 19) with 2/10 and 8/19, respectively. Thus, our results indicate that GlpQ-based B. miyamotoi serology holds neither specificity nor sensitivity.

scholarly journals Surveillance of Ticks and Tick-Borne Pathogens in Suburban Natural Habitats of Central Maryland

2021 ◽  
Author(s):  
Matthew T Milholland ◽  
Lars Eisen ◽  
Robyn M Nadolny ◽  
Andrias Hojgaard ◽  
Erika T Machtinger ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Spotted Fever ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Spotted Fever Group ◽  
Borrelia Miyamotoi ◽  
Natural Habitats ◽  
Questing Ticks ◽  
Tick Vectors

Abstract Lyme and other tick-borne diseases are increasing in the eastern United States and there is a lack of research on integrated strategies to control tick vectors. Here we present results of a study on tick-borne pathogens detected from tick vectors and rodent reservoirs from an ongoing 5-yr tick suppression study in the Lyme disease-endemic state of Maryland, where human-biting tick species, including Ixodes scapularis Say (Acari: Ixodidae) (the primary vector of Lyme disease spirochetes), are abundant. During the 2017 tick season, we collected 207 questing ticks and 602 ticks recovered from 327 mice (Peromyscus spp. (Rodentia: Cricetidae)), together with blood and ear tissue from the mice, at seven suburban parks in Howard County. Ticks were selectively tested for the presence of the causative agents of Lyme disease (Borrelia burgdorferi sensu lato [s.l.]), anaplasmosis (Anaplasma phagocytophilum), babesiosis (Babesia microti), ehrlichiosis (Ehrlichia ewingii, Ehrlichia chaffeensis, and ‘Panola Mountain’ Ehrlichia) and spotted fever group rickettsiosis (Rickettsia spp.). Peromyscus ear tissue and blood samples were tested for Bo. burgdorferi sensu stricto (s.s), A. phagocytophilum, Ba. microti, and Borrelia miyamotoi. We found 13.6% (15/110) of questing I. scapularis nymphs to be Bo. burgdorferi s.l. positive and 1.8% (2/110) were A. phagocytophilum positive among all sites. Borrelia burgdorferi s.s. was found in 71.1% (54/76) of I. scapularis nymphs removed from mice and 58.8% (194/330) of captured mice. Results from study on tick abundance and pathogen infection status in questing ticks, rodent reservoirs, and ticks feeding on Peromyscus spp. will aid efficacy evaluation of the integrated tick management measures being implemented.

scholarly journals Detection of Borrelia burgdorferi Sensu Lato and Relapsing Fever Borrelia in Feeding Ixodes Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of Borrelia yangtzensis and Borrelia miyamotoi

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 846
Author(s):  
Alice C. C. Lau ◽  
Yongjin Qiu ◽  
Mohamed Abdallah Mohamed Moustafa ◽  
Ryo Nakao ◽  
Michito Shimozuru ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Primary Forest ◽  
Borrelia Miyamotoi ◽  
Relapsing Fever ◽  
Ixodes Ticks ◽  
Ixodes Granulatus ◽  
New Geographical Record ◽  
Etiological Agents ◽  
Malaysian Borneo

Members of the Borrelia burgdorferi sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and Borrelia miyamotoi is one of the relapsing fever Borrelia (RFB). Despite the serological evidence of LD in Malaysia, there has been no report from Sarawak, Malaysian Borneo. Thus, this study aimed to detect and characterize Borrelia in rodents and Ixodes ticks from primary forests and an oil palm (OP) plantation in Sarawak. Borrelia yangtzensis (a member of the Bbsl complex) was detected in 43.8% (14/32) of Ixodes granulatus; most of the positive ticks were from the OP plantation (13/14). Out of 56 rodents, B. yangtzensis was detected in four Rattus spp. from the OP plantation and B. miyamotoi was detected in one rodent, Sundamys muelleri, from the primary forest. Further, the positive samples of B. yangtzensis were randomly selected for multilocus sequence analysis (MLSA). The MLSA results of successfully amplified tick samples revealed a clustering with the sequences isolated from Japan and China. This study is the first evidence of B. miyamotoi, a known human pathogen in Malaysia, and B. yangtzensis, which is circulating in ticks and rodents in Sarawak, Malaysian Borneo, and presenting a new geographical record of the Borrelia spp.

scholarly journals Questing Ixodes ricinus ticks (Acari, Ixodidae) as a vector of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in an urban area of north-eastern Poland

2019 ◽  
Vol 78 (1) ◽  
pp. 113-126 ◽  
Author(s):  
Katarzyna Kubiak ◽  
Janina Dziekońska-Rynko ◽  
Hanna Szymańska ◽  
Dariusz Kubiak ◽  
Małgorzata Dmitryjuk ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Area ◽  
Ixodes Ricinus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Borrelia Miyamotoi ◽  
North Eastern ◽  
Ixodes Ricinus Ticks

Neues zu zeckenübertragenen Infektionen

2017 ◽  
Vol 142 (11) ◽  
pp. 805-810
Author(s):  
Johannes Borde ◽  
Gerhard Dobler ◽  
Siegbert Rieg
Keyword(s):  
Borrelia Burgdorferi ◽  
Francisella Tularensis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Borrelia Miyamotoi ◽  
Emerging Pathogen

Was ist neu? Frühsommer-Meningoenzephalitis (FSME) In Europa nimmt die Ausbreitung von FSME in Richtung Westen zu. Erstmalig ist 2016 ein autochthoner humaner FSME-Fall in den Niederlanden dokumentiert worden. Die FSME-Erkrankungsfälle im Landkreis Reutlingen rücken die alimentäre FSME-Transmission in Deutschland wieder in das Bewusstsein. Tularämie (Hasenpest) Francisella tularensis hat ein breites Wirtsspektrum, von Kleinsäugern bis hin zu Arthropoden, wie z. B. Schildzecken. Die Transmission durch Zeqcken wurde bisher wenig beachtet. Vor dem Hintergrund neuerer epidemiologischen Erkenntnisse sollte auch nach Zeckenstichen mit entsprechenden Symptomen eine Francisella tularensis Infektion in Erwägung gezogen werden. Persistierende Beschwerden bei Borreliose (Borrelia-burgdorferi-sensu-lato-Komplex) Eine aktuelle Studie untersuchte den Effekt einer verlängerten antiinfektiven Behandlungsdauer auf persistierende Symptome, die mit einer Borrelieninfektion (Borrelia-burgdorferi-sensu-lato-Komplex) in Verbindung gebracht werden können. Die Therapie über 2 Wochen führte zu einer quantitativ gleichen und ebenso nachhaltigen Zunahme der Lebensqualität wie ein verlängertes Schema. Borrelia miyamotoi ist ein „Emerging Pathogen“ – erstmalig konnte nun auch in Deutschland ein humaner Infektionsfall nachgewiesen werden.

scholarly journals Borrelia burgdorferi and Borrelia miyamotoi seroprevalence in California blood donors

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243950
Author(s):  
Sharon I. Brummitt ◽  
Anne M. Kjemtrup ◽  
Danielle J. Harvey ◽  
Jeannine M. Petersen ◽  
Christopher Sexton ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Western Blot ◽  
Sierra Nevada ◽  
Blood Donors ◽  
Test System ◽  
Emerging Diseases ◽  
Borrelia Miyamotoi ◽  
Increased Risk ◽  
Significant Difference

The western blacklegged tick, Ixodes pacificus, an important vector in the western United States of two zoonotic spirochetes: Borrelia burgdorferi (also called Borreliella burgdorferi), causing Lyme disease, and Borrelia miyamotoi, causing a relapsing fever-type illness. Human cases of Lyme disease are well-documented in California, with increased risk in the north coastal areas and western slopes of the Sierra Nevada range. Despite the established presence of B. miyamotoi in the human-biting I. pacificus tick in California, clinical cases with this spirochete have not been well studied. To assess exposure to B. burgdorferi and B. miyamotoi in California, and to address the hypothesis that B. miyamotoi exposure in humans is similar in geographic range to B. burgdorferi, 1,700 blood donor sera from California were tested for antibodies to both pathogens. Sampling was from high endemic and low endemic counties for Lyme disease in California. All sera were screened using the C6 ELISA. All C6 positive and equivocal samples and nine randomly chosen C6 negative samples were further analyzed for B. burgdorferi antibody using IgG western blot and a modified two ELISA test system and for B. miyamotoi antibody using the GlpQ ELISA and B. miyamotoi whole cell sonicate western blot. Of the 1,700 samples tested in series, eight tested positive for antibodies to B. burgdorferi (0.47%, Exact 95% CI: 0.20, 0.93) and two tested positive for antibodies to B. miyamotoi (0.12%, Exact 95% CI: 0.01, 0.42). There was no statistically significant difference in seroprevalence for either pathogen between high and low Lyme disease endemic counties. Our results confirm a low frequency of Lyme disease and an even lower frequency of B. miyamotoi exposure among adult blood donors in California; however, our findings reinforce public health messaging that there is risk of infection by these emerging diseases in the state.

scholarly journals Impact of Strain Heterogeneity on Lyme Disease Serology in Europe: Comparison of Enzyme-Linked Immunosorbent Assays Using Different Species of Borrelia burgdorferi Sensu Lato

1998 ◽  
Vol 36 (2) ◽  
pp. 427-436 ◽  
Author(s):  
Ulrike Hauser ◽  
Heide Krahl ◽  
Helmut Peters ◽  
Volker Fingerle ◽  
Bettina Wilske
Keyword(s):  
Borrelia Burgdorferi ◽  
Blood Donors ◽  
Surface Protein ◽  
Sensu Stricto ◽  
Immunoglobulin M ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Tests ◽  
Western Blots ◽  
Previous Infection ◽  
Immunosorbent Assays

For the standardization of serological tests for Lyme borreliosis (LB) in Europe, the influence of the heterogeneity of Borrelia burgdorferi sensu lato must be assessed in detail. For this study four immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assays (ELISAs) with octyl-β-d-glucopyranoside extracts of strains PKo (Borrelia afzelii), PBi (Borrelia garinii), and PKa2 and B31 (both B. burgdorferi sensu stricto) were compared. Strains PKo, PBi, and PKa2 at the passages used for antigen preparations abundantly expressed outer surface protein C (OspC), whereas strain B31 at the passage used for antigen preparation did not express OspC. Sera (all from Germany) from 222 patients with clinically defined LB of all stages, 133 blood donors, and 458 forest workers were tested. None of the forest workers had symptoms consistent with LB at the time that the samples were collected. For IgM tests, receiver operating characteristic curves demonstrated that discrimination between sera from patients and blood donors was best with strain PKo and worst with strain B31. The discriminatory abilities of the four IgG ELISAs were similar in a diagnostically reasonable specificity range (90 to 100%). More than 20% of the sera from forest workers reacted strongly in the PKo IgG ELISA (optical density value, >1.5; other assays, less than 8%). Western blots of the sera with the most discrepant ELISA results revealed almost exclusive reactivity with p17. This highly immunogenic antigen is only expressed by strain PKo. This observation might be important for the development of assays enabling discrimination between asymptomatic or previous infection and active disease.

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