scholarly journals Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation

2020 ◽  
Vol 8 (11) ◽  
pp. 1757
Author(s):  
Inés Reigada ◽  
Clara Guarch-Pérez ◽  
Jayendra Z. Patel ◽  
Martijn Riool ◽  
Kirsi Savijoki ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Cells ◽  
Polymorphonuclear Leukocytes ◽  
Implantable Devices ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Endotracheal Tubes ◽  
The One ◽  
Implantable Titanium ◽  
And Staphylococcus Aureus

Nosocomial diseases represent a huge health and economic burden. A significant portion is associated with the use of medical devices, with 80% of these infections being caused by a bacterial biofilm. The insertion of a foreign material usually elicits inflammation, which can result in hampered antimicrobial capacity of the host immunity due to the effort of immune cells being directed to degrade the material. The ineffective clearance by immune cells is a perfect opportunity for bacteria to attach and form a biofilm. In this study, we analyzed the antibiofilm capacity of three naturally derived biofilm inhibitors when combined with immune cells in order to assess their applicability in implantable titanium devices and low-density polyethylene (LDPE) endotracheal tubes. To this end, we used a system based on the coculture of HL-60 cells differentiated into polymorphonuclear leukocytes (PMNs) and Staphylococcus aureus (laboratory and clinical strains) on titanium, as well as LDPE surfaces. Out of the three inhibitors, the one coded DHA1 showed the highest potential to be incorporated into implantable devices, as it displayed a combined activity with the immune cells, preventing bacterial attachment on the titanium and LDPE. The other two inhibitors seemed to also be good candidates for incorporation into LDPE endotracheal tubes.

Interaction of implant infection-related commensal bacteria with mesenchymal stem cells: A comparison between Cutibacterium acnes and Staphylococcus aureus

2021 ◽  
Author(s):  
Taghrid S El-Mahdy ◽  
Céline Mongaret ◽  
Jennifer Varin-Simon ◽  
Fabien Lamret ◽  
Véronique Vernet-Garnier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Staphylococcus Aureus ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Regenerative Process ◽  
Bacterial Biofilm ◽  
Biofilm Production ◽  
Cutibacterium Acnes ◽  
Live Bacteria ◽  
And Staphylococcus Aureus

Abstract Staphylococcus aureus and Cutibacterium acnes are involved in several tissue infections and can encounter mesenchymal stem cells (MSCs) during their role in tissue regenerative process. C. acnes and S. aureus internalization by three types of MSCs derived from bone marrow, dental pulp and Wharton's jelly; and bacterial biofilm production were compared. Internalization rates ranged between 1.7%-6.3% and 0.8%-2.7% for C. acnes and S. aureus, respectively. While C. acnes strains exhibited limited cytotoxic effect on MSCs, S. aureus were more virulent with marked effect starting after only three hours of interaction. Both bacteria were able to produce biofilms with respectively aggregated and monolayered structures for C. acnes and S. aureus. The increase in C. acnes capacity to develop biofilm following MSCs’ internalization was not linked to the significant increase in number of live bacteria, except for bone marrow-MSCs/C. acnes CIP 53.117 with 79% live bacteria compared to the 36% before internalization. On the other hand, internalization of S. aureus had no impact on its ability to form biofilms composed mainly of living bacteria. The present study underlined the complexity of MSCs-bacteria cross-interaction and brought insights into understanding the MSCs behaviour in response to bacterial infection in tissue regeneration context.

scholarly journals Aktivitas Antibakteri Isolat Bakteri Endofit dari Tanaman Miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 1 (1) ◽  
pp. 45-50
Author(s):  
Dwi Endah Kusumawati ◽  
Fachriyan Hasmi Pasaribu ◽  
Maria Bintang
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Bioactive Compound ◽  
Antimicrobial Compounds ◽  
E Coli ◽  
Activity Test ◽  
The One ◽  
And Staphylococcus Aureus

Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolate and test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. against two pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophytic bacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolates of endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus, whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.

scholarly journals Antibacterial potential of marine fungus Aspergillus nomius isolated from green algae Bornetella sp

2021 ◽  
Vol 9 (1) ◽  
pp. 49
Author(s):  
Deiske Adeliene Sumilat ◽  
Rosita A. J. Lintang
Keyword(s):  
Staphylococcus Aureus ◽  
Green Algae ◽  
Marine Fungi ◽  
Marine Fungus ◽  
Diffusion Method ◽  
Antibacterial Compounds ◽  
Aspergillus Nomius ◽  
Planting Method ◽  
The One ◽  
And Staphylococcus Aureus

Isolation of marine fungi symbiont of green algae Bornetella sp as a producer of antibacterial compounds has been carried out. This study aims to obtain symbiont fungi from green algae Bornetella sp which produces antibacterial compounds. The symbiont fungus was isolated using the direct planting method. Screening for the antibacterial activity of pure symbiont fungi isolates against Escheriscia coli and Staphylococcus aureus, and was carried out by the agar diffusion method. The isolation results obtained 4 isolates, but the one with the most potential to inhibit the growth of the tested bacteria was MFALM2. Molecular characterization showed that the MFALM2 isolate was identified as Aspergillus nomius with a 100% closeness level. Keywords: isolation; marine fungi; Aspergillus nomius; green alga; Bornetella sp. AbstrakIsolasi jamur laut yang bersimbion dengan alga hijau Bornetella sp sebagai penghasil senyawa antibakteri telah dilakukan. Penelitian ini bertujuan untuk memperoleh jamur simbion dari alga hijau Bornetella sp yang menghasilkan senyawa antibakteri. Isolasi jamur simbion dilakukan dengan metode direct planting. Skrining aktivitas antibakteri isolat murni jamur simbion terhadap bakteri uji Escheriscia coli dan Staphylococcus aureus dilakukan dengan metode difusi agar.  Hasil isolasi diperoleh 4 isolat, namun yang paling berpotensi menghambat pertumbuhan bakteri uji adalah MFALM2. Karakterisasi molekuler menunjukkan bahwa isolat MFALM2 teridentifikasi sebagai Aspergillus nomius dengan tingkat keeratan sebesar 100%.Kata kunci: isolasi; jamur lau; Aspergillus nomius; alga hijau; Bornetella sp.

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