scholarly journals Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control Xanthomonas campestris pv. campestris on Cabbage Seeds

2020 ◽  
Vol 8 (10) ◽  
pp. 1606
Author(s):  
Akikazu Sakudo ◽  
Makoto Haritani ◽  
Koichi Furusaki ◽  
Rumiko Onishi ◽  
Takashi Onodera
Keyword(s):  
Xanthomonas Campestris ◽  
Water Immersion ◽  
Hot Water ◽  
Stem Length ◽  
Black Rot ◽  
Distilled Water ◽  
Decimal Reduction Time ◽  
Significant Difference ◽  
Hydrogen Carbonate ◽  
Xanthomonas Campestris Pv Campestris

Xanthomonas campestris pv. campestris (Xcc) is an important seed-borne bacterial pathogen that causes black rot in brassica. Current seed disinfection methods for Xcc have disadvantages; chemical treatment has associated environmental risks, hot water immersion reduces germination, and dry heat treatment is protracted. Here, we treated Xcc-contaminated seeds with CAC-717, a recently developed disinfectant produced by applying an electric field and water flow to distilled water containing calcium hydrogen carbonate to produce mesoscopic crystals. The decimal reduction time (D-value) of Xcc suspension (8.22 log10 colony forming units (CFU)/mL) by CAC-717 treatment was 0.319 min. Treatment of Xcc-contaminated cabbage seeds at 25 °C for 30 min with CAC-717 significantly reduced bacterial cell numbers recovered from the seeds (0.36 log10 CFU/mL (SEM (standard error of the mean) = 0.23 log10 CFU/mL)) compared with distilled water treatment (3.52 log10 CFU/mL (SEM = 0.12 log10 CFU/mL)). Moreover, there was a lower incidence of black rot after treatment with CAC-717 (26.67% ± 3.33%) versus distilled water (56.67% ± 8.82%). For non-contaminated seeds, there was no significant difference in germination rate and plant stem length between distilled water and CAC-717 treatment after 5 days of cultivation. In conclusion, CAC-717 is a promising seed disinfectant without deleterious effects on germination or plant growth.

scholarly journals Antagonism of yeasts to Xanthomonas campestris pv. campestris on cabbage phylloplane in field

1999 ◽  
Vol 30 (3) ◽  
pp. 191-195 ◽  
Author(s):  
Sayonara M.P. Assis ◽  
Rosa L.R. Mariano ◽  
Sami J. Michereff ◽  
Gil Silva ◽  
Elizabeth A.A. Maranhão
Keyword(s):  
Disease Severity ◽  
Xanthomonas Campestris ◽  
Antagonistic Activity ◽  
Black Rot ◽  
Significant Difference ◽  
Xanthomonas Campestris Pv Campestris

Twenty yeast isolates, obtained from cabbage phylloplane, were evaluated for antagonistic activity against Xanthomonas campestris pv. campestris, in field. Plants of cabbage cv. Midori were pulverized simultaneously with suspensions of antagonists and pathogen. After 10 days, plants were evaluated through percentage of foliar area with lesions. Percentage of disease severity reduction (DSR%) was also calculated. Yeast isolates LR32, LR42 and LR19 showed, respectively, 72, 75 and 79% of DSR. These antagonists were tested in seven different application periods in relation to pathogen inoculation (T1=4 d before; T2=simultaneously; T3=4 d after; T4=4 d before + simultaneously; T5=4 d after + simultaneously; T6=4 d before + 4 d after; T7=4 d before + simultaneously + 4 d after). The highest DSRs were showed by LR42 (71%), LR42 (67%), LR35 (69%) and LR19 (68%) in the treatments T7, T4, T5 and T6, which significantly differed from the others. The same yeast antagonists were also tested for black rot control using different cabbage cultivars (Fuyutoyo, Master-325, Matsukaze, Midori, Sekai I and Red Winner). The DSRs varied from 58 to 61%, and there was no significant difference among cultivars.

scholarly journals In vitro evaluation for the efficacy of the chemicals/ antibiotics, botanicals and bioagents against black rot (Xanthomonas campestris pv. campestris) of cauliflower in plains of Kerala

2021 ◽  
Vol 16 (1) ◽  
pp. 59-63
Author(s):  
S. Lakshmi Prasanna ◽  
S. Ravi
Keyword(s):  
Xanthomonas Campestris ◽  
Disease Incidence ◽  
Garlic Extract ◽  
Black Rot ◽  
Copper Oxychloride ◽  
Copper Hydroxide ◽  
Turmeric Extract ◽  
Significant Difference ◽  
Xanthomonas Campestris Pv Campestris

Cauliflower (Brassica oleracea var botrytis) is one of the important cool season vegetable crop grown in India. One of the major disease which causes huge loss (50-70%) is black rot of cauliflower caused by Xanthomonas campestris pv. Campestris. In the present studyin vitro evaluation of chemicals/ antibiotics, botanicals and bio agents were studied and best selected treatments were studied at field level. Two antibiotics-Streptocycline, Tetracycline, (100,200,250ppm); Two chemicals- Copper oxychloride, Copper hydroxide, (0.15%, 0.2%); one combination of antibiotic and chemical Copper oxychloride + Streptocycline (0.15%+100ppm). Four botanicals leaf extract of tea, tea waste decoction, garlic extract, turmeric extract at two different concentrations 5 and 10 per cent and two bioagents Bacillus subtilis and Pseudomonas fluoresces were tested for their efficacy against Xanthomonas campestris pv. Campestris. The results were garlic extract 10 per cent was found to be effective against the pathogen followed by tetracycline 250 ppm. The untreated control recorded the maximum disease incidence, followed by turmeric extract 10 per cent application. But there observed no significant difference among the treatments Tetracycline 250, 200 ppm, P. fluorescens, copper hydroxide 0.2%, garlic extract 5 per cent and turmeric extract 10 per cent are found to be on par.

Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment

2021 ◽  
Vol 27 ◽  
pp. 102284
Author(s):  
Jakub Pečenka ◽  
Zuzana Bytešníková ◽  
Tomáš Kiss ◽  
Eliška Peňázová ◽  
Miroslav Baránek ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Water Treatment ◽  
Xanthomonas Campestris ◽  
Hot Water ◽  
Hot Water Treatment ◽  
Xanthomonas Campestris Pv Campestris

Improved control of black rot of broccoli caused by Xanthomonas campestris pv. campestris using a bacteriophage and a nonpathogenic Xanthomonas sp. strain

2017 ◽  
Vol 83 (6) ◽  
pp. 373-381 ◽  
Author(s):  
Hirofumi Nagai ◽  
Noriyuki Miyake ◽  
Shinro Kato ◽  
Daisuke Maekawa ◽  
Yasuhiro Inoue ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Black Rot ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Characterization of Xanthomonas campestris pv. campestris in Algeria

2021 ◽  
Vol 60 (1) ◽  
pp. 51-62
Author(s):  
Samia LAALA ◽  
Sophie CESBRON ◽  
Mohamed KERKOUD ◽  
Franco VALENTINI ◽  
Zouaoui BOUZNAD ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Housekeeping Genes ◽  
First Record ◽  
Black Rot ◽  
Physiological And Biochemical Characteristics ◽  
Main Production ◽  
Production Areas ◽  
Cruciferous Plants ◽  
Xanthomonas Campestris Pv Campestris

Xanthomonas campestris pv. campestris (Xcc) causes the black rot of cruciferous plants. This seed-borne bacterium is considered as the most destructive disease to cruciferous crops. Although sources of contamination are various, seeds are the main source of transmission. Typical symptoms of black rot were first observed in 2011 on cabbage and cauliflower fields in the main production areas of Algeria. Leaf samples displaying typical symptoms were collected during 2011 to 2014, and 170 strains were isolated from 45 commercial fields. Xcc isolates were very homogeneous in morphological, physiological and biochemical characteristics similar to reference strains, and gave positive pathogenicity and molecular test results (multiplex PCR with specific primers). This is the first record of Xcc in Algeria. Genetic diversity within the isolates was assessed in comparison with strains isolated elsewhere. A multilocus sequence analysis based on two housekeeping genes (gyrB and rpoD) was carried out on 77 strains representative isolates. The isolates grouped into 20 haplotypes defined with 68 polymorphic sites. The phylogenetic tree obtained showed that Xcc is in two groups, and all Algerian strains clustered in group 1 in three subgroups. No relationships were detected between haplotypes and the origins of the seed lots, the varieties of host cabbage, the years of isolation and agroclimatic regions.

scholarly journals The effect of temperature change on fluoride uptake from a mouthrinse by enamel specimens

2012 ◽  
Vol 06 (04) ◽  
pp. 361-369 ◽  
Author(s):  
Serdar Baglar ◽  
Adil Nalcaci ◽  
Mustafa Tastekin
Keyword(s):  
Body Temperature ◽  
Room Temperature ◽  
Hot Water ◽  
Effect Of Temperature ◽  
Distilled Water ◽  
Fluoride Solution ◽  
Fluoride Uptake ◽  
Significant Difference ◽  
Human Body Temperature ◽  
Different Temperatures

ABSTRACTObjective: The aim of this study was to examine the effect of temperature on fluoride uptake by enamel specimens from a 0.05% NaF-fluoridated mouthrinse (Oral-B Advantage; Oral-B Laboratories, Newbridge, UK).Methods: Enamel specimens were prepared from extracted human maxillary central incisors. A fluoride-specific ion electrode was used to measure the uptake from a 2 ppm fluoride solution containing 50.0 mL of distilled water, total ion strength adjustment buffer, and fluoridated rinse at 3 different temperatures (room temperature, 25°C; human body temperature, 37°C; hyper-fever temperature, 43°C). One-way analysis of variance and least significant difference were used to assess intragroup and intergroup differences (P<.05).Results: The study found that both the amount and the rate of fluoride uptake increased significantly with increase in temperature. This effect was particularly noticeable at 43°C.Conclusions: The temperature of the NaF mouthrinse may easily and safely be increased beyond room temperature by placing a container of the NaF mouthrinse in a bowl of hot water, allowing greater fluoride penetration into the enamel from the mouthrinse when used at home as a routine prophylactic agent. (Eur J Dent 2012;6:361-369)

Involvement of a novel peroxidase isozyme and lignification in hydathodes in resistance to black rot disease in cabbage

2000 ◽  
Vol 78 (9) ◽  
pp. 1144-1149 ◽  
Author(s):  
P A Gay ◽  
S Tuzun
Keyword(s):  
Xanthomonas Campestris ◽  
Virulent Strain ◽  
Black Rot ◽  
Peroxidase Isozyme ◽  
Anionic Peroxidase ◽  
Peroxidase Isozymes ◽  
Resistant Varieties ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris ◽  
Lignin Deposition

The physiological mechanisms associated with resistance of cabbage to black rot disease seem to be associated with the hydathodes. To investigate the role of hydathodes in disease resistance, total peroxidase activities, anionic peroxidase isozyme expression, and lignin deposition were determined in hydathodes of resistant (Hancock and Green Cup), partially resistant (Cheers), and susceptible (Strukton and Perfect Ball) cabbage varieties (Brassica oleracea L.) during pathogenesis with Xanthomonas campestris pv. campestris. Four-week-old plants were petiole-inoculated with a highly virulent strain of X. campestris pv. campestris (FD91L). Hydathodal fluids were collected daily over a 14-day period from infected plants as well as noninfected, mock-inoculated control plants. Hydathodal fluids of resistant varieties had greater peroxidase activity when compared to susceptible ones, with infected plants having higher peroxidase levels than noninfected plants. Isoelectric focusing revealed the presence of four anionic peroxidase isozymes in hydathodal fluids, with the most anionic one (pI of 3.6) accumulating only upon infection. Lignin deposition in and around the hydathodes was associated with the accumulation of this particular isozyme in hydathodal fluids. The evidence suggests that a rapid, systemic response is associated with resistance to the bacterial pathogen.Key words: peroxidases, hydathodes, isozymes, black rot disease, cabbage, Xanthomonas campestris pv. campestris.

Sign in / Sign up

Export Citation Format

Share Document