scholarly journals Microbiota of the Therapeutic Euganean Thermal Muds with a Focus on the Main Cyanobacteria Species

2020 ◽  
Vol 8 (10) ◽  
pp. 1590
Author(s):  
Barbara Gris ◽  
Laura Treu ◽  
Raffaella Margherita Zampieri ◽  
Fabrizio Caldara ◽  
Chiara Romualdi ◽  
...  
Keyword(s):  
Nanopore Sequencing ◽  
Maturation Process ◽  
Target Species ◽  
Population Composition ◽  
Chl A ◽  
Natural Clays ◽  
Therapeutic Properties ◽  
Two Parameters ◽  
Inflammatory Molecules

The Euganean Thermal District has been known since Roman times for the therapeutic properties of peloids, obtained from natural clays that have undergone a traditional maturation process. This leads to the growth of a green microbial biofilm with Cyanobacteria and the target species Phormidium sp. ETS-05 as fundamental components for their ability to synthetize anti-inflammatory molecules. Currently, in-depth studies on the microbiota colonizing Euganean peloids, as in general on peloids utilized worldwide, are missing. This is the first characterization of the microbial community of Euganean thermal muds, also investigating the effects of environmental factors on its composition. We analysed 53 muds from 29 sites (Spas) using a polyphasic approach, finding a stable microbiota peculiar to the area. Differences among mud samples mainly depended on two parameters: water temperature and shading of mud maturation plants. In the range 37–47 °C and in the case of irradiance attenuation due to the presence of protective roofs, a statistically significant higher mud Chl a content was detected. Moreover, in these conditions, a characteristic microbial and Cyanobacteria population composition dominated by Phormidium sp. ETS-05 was observed. We also obtained the complete genome sequence of this target species using a mixed sequencing approach based on Illumina and Nanopore sequencing.

Chemical characterization of tequila maturation process and their connection with the physicochemical properties of the cask

2021 ◽  
Vol 98 ◽  
pp. 103804
Author(s):  
Walter M. Warren-Vega ◽  
Rocío Fonseca-Aguiñaga ◽  
Linda V. González-Gutiérrez ◽  
Francisco Carrasco-Marín ◽  
Ana I. Zárate-Guzmán ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Chemical Characterization ◽  
Maturation Process

scholarly journals Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes?

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 396
Author(s):  
Ewa Sajnaga ◽  
Marcin Skowronek ◽  
Agnieszka Kalwasińska ◽  
Waldemar Kazimierczak ◽  
Karolina Ferenc ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Entomopathogenic Nematodes ◽  
Bacterial Species ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Nanopore Sequencing ◽  
Bacterial Phyla ◽  
Melolontha Melolontha

This study focused on the potential relationships between midgut microbiota of the common cockchafer Melolontha melolontha larvae and their resistance to entomopathogenic nematodes (EPN) infection. We investigated the bacterial community associated with control and unsusceptible EPN-exposed insects through nanopore sequencing of the 16S rRNA gene. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the most abundant bacterial phyla within the complex and variable midgut microbiota of the wild M. melolontha larvae. The core microbiota was found to include 82 genera, which accounted for 3.4% of the total number of identified genera. The EPN-resistant larvae differed significantly from the control ones in the abundance of many genera belonging to the Actinomycetales, Rhizobiales, and Clostridiales orders. Additionally, the analysis of the microbiome networks revealed different sets of keystone midgut bacterial genera between these two groups of insects, indicating differences in the mutual interactions between bacteria. Finally, we detected Xenorhabdus and Photorhabdus as gut residents and various bacterial species exhibiting antagonistic activity against these entomopathogens. This study paves the way to further research aimed at unravelling the role of the host gut microbiota on the output of EPN infection, which may contribute to enhancement of the efficiency of nematodes used in eco-friendly pest management.

scholarly journals Singular perturbations of the unicritical polynomials with two parameters

2016 ◽  
Vol 37 (6) ◽  
pp. 1997-2016 ◽  
Author(s):  
YINGQING XIAO ◽  
FEI YANG
Keyword(s):  
Julia Set ◽  
Dynamical Behavior ◽  
Rational Maps ◽  
Topological Properties ◽  
Fatou Set ◽  
The Family ◽  
Image Position ◽  
Two Parameters ◽  
Unicritical Polynomials

In this paper, we study the dynamics of the family of rational maps with two parameters $$\begin{eqnarray}f_{a,b}(z)=z^{n}+\frac{a^{2}}{z^{n}-b}+\frac{a^{2}}{b},\end{eqnarray}$$ where $n\geq 2$ and $a,b\in \mathbb{C}^{\ast }$. We give a characterization of the topological properties of the Julia set and the Fatou set of $f_{a,b}$ according to the dynamical behavior of the orbits of the free critical points.

Non-photochemical quenching of chlorophyll a fluorescence: early history and characterization of two xanthophyll-cycle mutants of Chlamydomonas reinhardtii

2002 ◽  
Vol 29 (10) ◽  
pp. 1141 ◽  
Author(s):  
Govindjee ◽  
Manfredo J. Seufferheld
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Oxygen Evolution ◽  
Xanthophyll Cycle ◽  
Photochemical Quenching ◽  
Chl A ◽  
Fluorescence Transient ◽  
Chl A Fluorescence ◽  
Non Photochemical Quenching

This paper deals first with the early, although incomplete, history of photoinhibition, of 'non-QA-related chlorophyll (Chl) a fluorescence changes', and the xanthophyll cycle that preceded the discovery of the correlation between non-photochemical quenching of Chl a fluorescence (NPQ) and conversion of violaxanthin to zeaxanthin. It includes the crucial observation that the fluorescence intensity quenching, when plants are exposed to excess light, is indeed due to a change in the quantum yield of fluorescence. The history ends with a novel turn in the direction of research — isolation and characterization of NPQ xanthophyll-cycle mutants of Chlamydomonas reinhardtii Dangeard and Arabidopsis thaliana (L.) Heynh., blocked in conversion of violaxanthin to zeaxanthin, and zeaxanthin to violaxanthin, respectively. In the second part of the paper, we extend the characterization of two of these mutants (npq1, which accumulates violaxanthin, and npq2, which accumulates zeaxanthin) through parallel measurements on growth, and several assays of PSII function: oxygen evolution, Chl a fluorescence transient (the Kautsky effect), the two-electron gate function of PSII, the back reactions around PSII, and measurements of NPQ by pulse-amplitude modulation (PAM 2000) fluorimeter. We show that, in the npq2 mutant, Chl a fluorescence is quenched both in the absence and presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). However, no differences are observed in functioning of the electron-acceptor side of PSII — both the two-electron gate and the back reactions are unchanged. In addition, the role of protons in fluorescence quenching during the 'P-to-S' fluorescence transient was confirmed by the effect of nigericin in decreasing this quenching effect. Also, the absence of zeaxanthin in the npq1 mutant leads to reduced oxygen evolution at high light intensity, suggesting another protective role of this carotenoid. The available data not only support the current model of NPQ that includes roles for both pH and the xanthophylls, but also are consistent with additional protective roles of zeaxanthin. However, this paper emphasizes that we still lack sufficient understanding of the different parts of NPQ, and that the precise mechanisms of photoprotection in the alga Chlamydomonas may not be the same as those in higher plants.

scholarly journals Characterization of small-scale fisheries in the Camamu-Almada basin, southeast state of Bahia, Brazil

2008 ◽  
Vol 68 (4) ◽  
pp. 711-719 ◽  
Author(s):  
TCM. Souza ◽  
M. Petrere-Jr
Keyword(s):  
Low Income ◽  
Small Scale ◽  
Income Generation ◽  
Target Species ◽  
Market Prices ◽  
Marine Fishery ◽  
Small Scale Fisheries ◽  
Main Factor ◽  
Stock Abundance

In the Camamu-Almada basin, marine fishery is exclusively small-scale, with several structural deficiencies such as boats with low or absent navigational technology, lack of credit and low income. Local fishers complain that shrimp and lobster trawling fishing is the main factor responsible for low stock abundance, but they still persist in these activities as these two species command the highest market prices. So they feel that the target species are already over-fished. We suggest that proper management action, alternative ways of income generation and the payment of job insurance would help to mitigate the problem.

scholarly journals Bending of an Infinite beam on a base with two parameters in the absence of a part of the base

2018 ◽  
Vol 33 ◽  
pp. 02074
Author(s):  
Maxim Aleksandrovskiy ◽  
Lidiya Zaharova
Keyword(s):  
Rapid Development ◽  
Fourier Integral ◽  
Infinite Length ◽  
Degenerate Kernel ◽  
Shift Parameter ◽  
Approximate Methods ◽  
Concentrated Force ◽  
High Rise ◽  
Two Parameters

Currently, in connection with the rapid development of high-rise construction and the improvement of joint operation of high-rise structures and bases models, the questions connected with the use of various calculation methods become topical. The rigor of analytical methods is capable of more detailed and accurate characterization of the structures behavior, which will affect the reliability of objects and can lead to a reduction in their cost. In the article, a model with two parameters is used as a computational model of the base that can effectively take into account the distributive properties of the base by varying the coefficient reflecting the shift parameter. The paper constructs the effective analytical solution of the problem of a beam of infinite length interacting with a two-parameter voided base. Using the Fourier integral equations, the original differential equation is reduced to the Fredholm integral equation of the second kind with a degenerate kernel, and all the integrals are solved analytically and explicitly, which leads to an increase in the accuracy of the computations in comparison with the approximate methods. The paper consider the solution of the problem of a beam loaded with a concentrated force applied at the point of origin with a fixed value of the length of the dip section. The paper gives the analysis of the obtained results values for various parameters of coefficient taking into account cohesion of the ground.

scholarly journals Persistent Liquidity Effects and Long-Run Money Demand

2014 ◽  
Vol 6 (2) ◽  
pp. 71-107 ◽  
Author(s):  
Fernando Alvarez ◽  
Francesco Lippi
Keyword(s):  
Interest Rates ◽  
Money Demand ◽  
Asset Markets ◽  
Propagation Mechanism ◽  
Monetary Model ◽  
Long Run ◽  
Short Run ◽  
The Stability ◽  
Two Parameters

We present a monetary model with segmented asset markets that implies a persistent fall in interest rates after a once-and-for-all increase in liquidity. The gradual propagation mechanism produced by our model is novel in the literature. We provide an analytical characterization of this mechanism, showing that the magnitude of the liquidity effect on impact, and its persistence, depend on the ratio of two parameters: the long-run interest rate elasticity of money demand and the intertemporal substitution elasticity. The model simultaneously explains the short-run “instability” of money demand estimates as well as the stability of long-run interest-elastic money demand. (JEL E13, E31, E41, E43, E52, E62)

scholarly journals The risk to fishery performance associated with spatially resolved management of Antarctic krill (Euphausia superba) harvesting

2009 ◽  
Vol 66 (10) ◽  
pp. 2148-2154 ◽  
Author(s):  
Simeon L. Hill ◽  
Philip N. Trathan ◽  
David J. Agnew
Keyword(s):  
Antarctic Krill ◽  
Euphausia Superba ◽  
Ecosystem Approach ◽  
Fishing Effort ◽  
Target Species ◽  
Ecosystem Models ◽  
Spatially Resolved ◽  
Management Actions ◽  
Antarctic Krill Euphausia Superba

Abstract Hill, S. L., Trathan, P. N., and Agnew, D. J. 2009. The risk to fishery performance associated with spatially resolved management of Antarctic krill (Euphausia superba) harvesting. – ICES Journal of Marine Science, 66: 2148–2154. The ecosystem approach to fisheries attempts to define objectives for target species, the wider ecosystem, and critically, the fishery itself. Proposals for implementing the approach often include spatial restrictions on harvesting, so it is important to understand how these will affect fishery performance. One metric of potential performance is the probability of encountering exploitable densities of a target species at the scale of fishing operations. The probability of encountering exploitable densities of Antarctic krill, Euphausia superba, at the scale of 1 nautical mile during an acoustic survey was predicted by bathymetry and the mean krill density at the larger scale at which the fishery is managed. This suggests that the risk to fishery performance will increase if management actions relocate the fishery into deeper water. The results also suggest that ecosystem models resolved to the spatial scale of management units could usefully predict effects at the scale of fishing operations. However, correct parameterization of these models will require better characterization of threshold densities for efficient exploitation. Finally, the distribution of catch and fishing effort over an entire fishing season reflected the distribution of krill density observed during the survey.

scholarly journals Identifying Métiers Using Landings Profiles: An Octopus-Driven Multi-Gear Coastal Fleet

2021 ◽  
Vol 9 (9) ◽  
pp. 1022
Author(s):  
Monika J. Szynaka ◽  
Karim Erzini ◽  
Jorge M. S. Gonçalves ◽  
Aida Campos
Keyword(s):  
Clustering Analysis ◽  
Limited Data ◽  
Target Species ◽  
Fishing Gears ◽  
Initial Characterization ◽  
Management Advice ◽  
South Portugal

The multi-gear coastal vessels in the Algarve (South Portugal) own licenses for various fishing gears. However, it is generally uncertain what gears they use, which is problematic as each individual gear is responsible for unique impacts on the resources and the environment. In this study, landing profiles identified for the multi-gear coastal fleet (2012–2016) were used as support in defining potential métiers using k-mean clustering analysis (CLARA) along with information from past studies on métiers. The results showed that more than 50% of the vessels were engaged in the octopus fishery year-round, using traps, while a small percentage (~13%) were entirely dedicated to clam dredging. In general, gillnets (21%) were used to target monkfish, hake and bastard soles, while trammel nets (6%) were used to target cuttlefish, with some vessels alternating the fishing gears (either seasonally or annually) according to target species. The method for the initial characterization of this fleet’s métiers and its efficiency with limited data is discussed, as well as the utility of this segmentation in support of management advice.

Development of a new empirical method for Tunnel Squeezing Classification (TSC)

2020 ◽  
Vol 53 (4) ◽  
pp. 655-660 ◽  
Author(s):  
Hadi Farhadian ◽  
Arash Nikvar-Hassani
Keyword(s):  
Quality Index ◽  
Empirical Method ◽  
Point Of View ◽  
Geological Hazard ◽  
Case Histories ◽  
Proposed Model ◽  
Two Parameters ◽  
Classification Tool ◽  
Selection Of

The characterization of squeezing phenomena as a geological hazard is of great importance because squeezing has a crucial role in the selection of the route and type of tunnels and in the characteristics of the excavation device. Tunnel squeezing is also the basis for the designation and construction of tunnelling-related structures. We present a new tunnel squeezing classification tool to predict tunnel squeezing based on two parameters: Q, the tunnelling quality index; and H, the depth of the tunnel. We used data collected from published papers to train the model; these data included 225 case histories from different countries, including Andorra, India, Iran, Japan, Nepal, Spain, Turkey and Venezuela. Validation of the model indicated that our tunnel squeezing classification tool is more accurate than the speculative and analytical methods currently in use. The proposed model will help tunnelling experts to classify tunnelling media from the point of view of squeezing hazards.

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