scholarly journals Re-Evaluation of the Order Sordariales: Delimitation of Lasiosphaeriaceae s. str., and Introduction of the New Families Diplogelasinosporaceae, Naviculisporaceae, and Schizotheciaceae

2020 ◽  
Vol 8 (9) ◽  
pp. 1430
Author(s):  
Yasmina Marin-Felix ◽  
Andrew N. Miller ◽  
José F. Cano-Lira ◽  
Josep Guarro ◽  
D. García ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Apical Cell ◽  
New Genera ◽  
Nuclear Rdna ◽  
Internal Transcribed Spacer Region ◽  
Natural Classification ◽  
Reference Strains

The order Sordariales includes the polyphyletic family Lasiosphaeriaceae, which comprises approximately 30 genera characterized by its paraphysate ascomata, asci with apical apparati, and mostly two-celled ascospores, which have a dark apical cell and a hyaline lower cell, frequently ornamented with mucilaginous appendages. To produce a more natural classification of this family, we carried out a phylogenetic analysis based on sequences of the internal transcribed spacer region (ITS), the nuclear rDNA large subunit (LSU), and fragments of ribosomal polymerase II subunit 2 (rpb2) and β-tubulin (tub2) genes of several isolates from soil and of reference strains of the Sordariales. As a result, Lasiosphaeriaceae s. str. has been circumscribed for the clade including the type species of the genus Lasiosphaeria and, consequently, its description emended. In addition, the new families Diplogelasinosporaceae, Naviculisporaceae, and Schizotheciaceae are introduced to accommodate those taxa located far from the Lasiosphaeriaceae s. str. Moreover, we propose the erection of the new genera Areotheca, Lundqvistomyces, Naviculispora, Pseudoechria, Pseudoschizothecium, and Rhypophila based on morphological and sequence data. New combinations for several species of the genera Cladorrhinum, Jugulospora, Podospora, Schizothecium, and Triangularia are proposed, their descriptions are emended, and dichotomous keys are provided to discriminate among their species.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 401 (3) ◽  
pp. 199
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

A new and intriguing brown-spored Leucocoprinus species

Phytotaxa ◽  
2021 ◽  
Vol 479 (1) ◽  
pp. 44-54
Author(s):  
JUAN M. SUAREZ ◽  
ALEXANDRA M. GOTTLIEB ◽  
BERNARDO E. LECHNER
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Buenos Aires ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nuclear Rdna ◽  
Internal Transcribed Spacer Region ◽  
A New Species

Leucocoprinus brunneosporus sp. nov., collected at the Santa Catalina Reserve (Buenos Aires, Argentina), is proposed as a new species. We performed separate phylogenetic analyses of the nuclear rDNA large subunit (28S) and the complete nuclear rDNA internal transcribed spacer region (ITS) to establish the placement of the new species in the phylogeny of the Agaricaceae. This new species has a macromorphology similar to that of Lc. birnbaumii, but the spore print is light brown. Detailed descriptions and illustrations of the macro- and microscopic characters are provided.

Kamalia indica, a new genus and species belonging to Diaporthales (Ascomycota, Fungi) described based on morphology and molecular phylogeny

Phytotaxa ◽  
2019 ◽  
Vol 425 (4) ◽  
pp. 233-243
Author(s):  
SHIWALI RANA ◽  
SANJAY KUMAR SINGH
Keyword(s):  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
New Genus ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Type Specimen ◽  
Morphological Description ◽  
Fungal Culture ◽  
Internal Transcribed Spacer Region

A fungal taxa isolated from leaf spots of Mallotus philippensis from Kangra district of North-Western Himalayan region of India is established as a new genus based on morphological characters of asexual-morphs, cultural characteristics and phylogenetic analyses of the partial nuclear ribosomal 28S large subunit (LSU) and internal transcribed spacer (ITS) rDNA sequence data. The generic placement of the genus has been determined based on DNA sequences from authenticated isolates. The present taxon has turned out to be distinct, showing nearly 90% identity with other known genera in Diaporthales based on nrDNA internal transcribed spacer region. The morphological description is provided for the new taxa and compared with the similar taxa belonging to the order Diaporthales. The culture was found to show heavy sporulation in all kind of media. The type specimen and ex type culture have been deposited in the Ajrekar Mycological Herbarium (AMH) and National Fungal Culture Collection of India (NFCCI-WDCM 932), respectively.

Tubaria keralensis, a new species of T. furfuracea complex from tropical India

Phytotaxa ◽  
2016 ◽  
Vol 278 (3) ◽  
pp. 287 ◽  
Author(s):  
K. P. DEEPNA LATHA ◽  
NEERAJA K. Paramban ◽  
PATINJAREVEETTIL MANIMOHAN
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Internal Transcribed Spacer Region ◽  
Comprehensive Description ◽  
Kerala State ◽  
A New Species

Tubaria keralensis sp. nov. is described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive description, photographs, and comparisons with phenetically similar and phylogenetically related species are provided. Tubaria keralensis is characterized by its strongly intervenose lamellae with concolorous edges; a stipe with velar remnants and a white, strigose basal mycelium and broadly ellipsoid to ovoid basidiospores. Nuclear ribosomal internal transcribed spacer region (nrITS) and nuclear ribosomal large subunit (nrLSU) gene region were sequenced and analyzed. Maximum likelihood (ML) analysis of combined nrITS and nrLSU sequence data revealed the novelty of the species as well as its placement in the T. furfuracea complex. The present discovery of T. keralensis forms the second report of a new Tubaria species from Kerala.

scholarly journals Extended phylogeny and a revised generic classification of thePannariaceae(Peltigerales, Ascomycota)

2014 ◽  
Vol 46 (5) ◽  
pp. 627-656 ◽  
Author(s):  
Stefan EKMAN ◽  
Mats WEDIN ◽  
Louise LINDBLOM ◽  
Per M. JØRGENSEN
Keyword(s):  
Data Model ◽  
Internal Transcribed Spacer ◽  
Nucleotide Diversity ◽  
Sequence Data ◽  
Species Level ◽  
Internal Transcribed Spacer Region ◽  
Original Sequence ◽  
Site Heterogeneity ◽  
Previously Treated

AbstractWe estimated phylogeny in the lichen-forming ascomycete familyPannariaceae. We specifically modelled spatial (across-site) heterogeneity in nucleotide frequencies, as models not incorporating this heterogeneity were found to be inadequate for our data. Model adequacy was measured here as the ability of the model to reconstruct nucleotide diversity per site in the original sequence data. A potential non-orthologue in the internal transcribed spacer region (ITS) ofDegelia plumbeawas observed. We propose a revised generic classification for thePannariaceae, accepting 30 genera, based on our phylogeny, previously published phylogenies, as well as available morphological and chemical data. Four genera are established as new:Austroparmeliella(for the ‘Parmeliella’laceratagroup), Nebularia(for the ‘Parmeliella’incrassatagroup),Nevesia(for ‘Fuscopannaria’sampaiana), andPectenia(for the ‘Degelia’plumbeagroup). Two genera are reduced to synonymy,Moelleropsis(included inFuscopannaria) andSantessoniella(non-monophyletic; type included inPsoroma).Lepidocollema, described as monotypic, is expanded to include 23 species, most of which have been treated in the ‘Parmeliella’marianagroup.HomotheciumandLeightoniella, previously treated in theCollemataceae, are here referred to thePannariaceae. We propose 41 new species-level combinations in the newly described and re-circumscribed genera mentioned above, as well as inLeciophysmaandPsoroma.

A morpho-molecular re-appraisal of Polystigma fulvum and P. rubrum (Polystigma, Polystigmataceae)

Phytotaxa ◽  
2019 ◽  
Vol 422 (3) ◽  
pp. 209-224
Author(s):  
DIGVIJAYINI BUNDHUN ◽  
RAJESH JEEWON ◽  
MONIKA C. DAYARATHNE ◽  
TIMUR S. BULGAKOV ◽  
ALEXANDER K. KHRAMTSOV ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Conidiogenous Cell ◽  
Nuclear Rdna ◽  
Asexual Morph ◽  
Dna Sequence Data ◽  
Sexual Morph ◽  
First Time

Collections of eleven Prunus specimens infected with Polystigma species from Belarus and Russia yielded two existing taxa: Polystigma fulvum (sexual morph) and Polystigma rubrum (asexual morph). DNA based phylogenies of large subunit nuclear rDNA (LSU) and nuclear ribosomal internal transcribed spacer (ITS) are provided for the first time for Polystigma fulvum and its placement is confirmed within Polystigmataceae. The concatenated LSU and ITS DNA sequence data for P. rubrum, analyzed to infer its potential relationship to other Polystigma species, also show that strains of P. rubrum are segregated into two subclades with sufficient genetic differences. No significant differences in morphology or morphometry among the strains of P. rubrum between the two subclades, especially vis-à-vis the conidiogenous cell and conidium sizes are observed (P>0.05). Subspecies concept of P. rubrum complex is discussed.

Species relationships between antifungal chitinase and nuclear rDNA (internal transcribed spacer) sequences in the genus Hordeum

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 339-347 ◽  
Author(s):  
Alfredo De Bustos ◽  
Yolanda Loarce ◽  
Nicolás Jouve
Keyword(s):  
Concerted Evolution ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Diploid Species ◽  
Species Relationships ◽  
Nuclear Rdna ◽  
Hordeum Murinum ◽  
Internal Transcribed Spacer Sequences ◽  
Antifungal Chitinase

The sequences of the chitinase gene (Chi-26) and the internal transcribed spacer of 18S – 5.8S – 26S rDNA (ITS1) were determined to analyze the phylogenetic relationships among species representing the four basic genomes of the genus Hordeum. Grouping analysis based on data for Chi-26 gene sequences placed Hordeum secalinum (H genome) near the Hordeum murinum complex (Xu genome), and Hordeum bulbosum distant from the other species that carried the I genome. ITS sequence data showed the expected grouping based on the genome classification of the species studied. Different sequences of ITS were detected even in the genomes of the diploid species. The results are interpreted in terms of defective or unfinished concerted evolution processes in each taxon.Key words: ITS, Hordeum, phylogeny, chitinase, concerted evolution.

Cortinarius rapaceoides, a new record for Turkey

Mycotaxon ◽  
2020 ◽  
Vol 135 (3) ◽  
pp. 559-568
Author(s):  
Meryem Senay Şengul Demirak ◽  
Hakan Işik
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
New Record ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Description ◽  
Molecular Evidence

A macrofungus collected from Akbelen village in Tokat has been identified based on molecular evidence and morphology as Cortinarius rapaceoides, a new record from Turkey. Sequence data generated from the nuclear ribosomal internal transcribed spacer and ribosomal large subunit regions were deposited in GenBank and used to determine phylogenetic relationships within Cortinarius subg. Phlegmacium. A morphological description, illustrations, and phylogeny of the Turkish C. rapaceoides are provided.

Phylogenetic analyses of Nematoctonus and Hohenbuehelia (Pleurotaceae)

2007 ◽  
Vol 85 (8) ◽  
pp. 762-773 ◽  
Author(s):  
Alexandra T.E. Koziak ◽  
Kei Chin Cheng ◽  
R. Greg Thorn
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nuclear Ribosomal Dna ◽  
Monophyletic Clade ◽  
Parasitoid Species ◽  
Internal Transcribed Spacer Region ◽  
Species Limits ◽  
Additional Gene

Hohenbuehelia (Agaricales, Pleurotaceae) and Nematoctonus (Hyphomycetes) are the names for the sexual and asexual stages of a genus of nematode-destroying fungi (Basidiomycota). We obtained partial sequences of nuclear ribosomal DNA, including the internal transcribed spacer region and the 5′ end of the large subunit, of 37 isolates of Hohenbuehelia and Nematoctonus representing 13 of the 16 described species in Nematoctonus. Phylogenetic analyses support Hohenbuehelia–Nematoctonus as a monophyletic clade of the Pleurotaceae, within which the species were placed in five main subclades. Exclusively predatory species ( Nematoctonus brevisporus Thorn & G.L. Barron, Nematoctonus campylosporus Drechsler, Nematoctonus robustus F.R. Jones, and Nematoctonus sp. UAMH 5317) appear to be basal. In these species, adhesive knobs to capture prey are produced on their hyphae but not on their conidia. A single mycelial individual may feed on many nematodes. From these have arisen both exclusively parasitoid species ( Nematoctonus cylindrosporus Thorn & G.L. Barron, Nematoctonus leiosporus Drechsler, Nematoctonus leptosporus Drechsler, Nematoctonus pachysporus Drechsler, Nematoctonus tylosporus Drechsler), and species that we call intermediate predators ( Nematoctonus angustatus Thorn & G.L. Barron, Nematoctonus concurrens Drechsler, Nematoctonus geogenius Thorn & GL. Barron, Nematoctonus hamatus Thorn & G.L. Barron, and Nematoctonus subreniformis Thorn & G.L. Barron). Exclusively parasitoid species have conidia that germinate to form sticky knobs that attach to passing nematodes but lack adhesive knobs on the hyphae. Each mycelial individual feeds on only one nematode. Intermediate predators have adhesive knobs both on hyphae and on germinated conidia and can act in both predatory and parasitoid modes. Most morphospecies are resolved as monophyletic, but sequences of additional gene regions are required to clarify species limits within the N. angustatus – N. geogenius group.

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