scholarly journals Generation of a Non-Transgenic Genetically Improved Yeast Strain for Wine Production from Nitrogen-Deficient Musts

2020 ◽  
Vol 8 (8) ◽  
pp. 1194 ◽  
Author(s):  
Eduardo Kessi-Pérez ◽  
Jennifer Molinet ◽  
Verónica García ◽  
Omayra Aguilera ◽  
Fernanda Cepeda ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
Direct Consequence ◽  
Nitrogen Addition ◽  
Wine Industry ◽  
Economic Losses ◽  
Improvement Program ◽  
Yeast Saccharomyces Cerevisiae ◽  
Wine Production ◽  
Grape Must ◽  
Improved Strain

The yeast Saccharomyces cerevisiae is the main species responsible for the process that involves the transformation of grape must into wine, with the initial nitrogen in the grape must being vital for it. One of the main problems in the wine industry is the deficiency of nitrogen sources in the grape must, leading to stuck or sluggish fermentations, and generating economic losses. In this scenario, an alternative is the isolation or generation of yeast strains with low nitrogen requirements for fermentation. In the present study, we carry out a genetic improvement program using as a base population a group of 70 strains isolated from winemaking environments mainly in Chile and Argentina (F0), making from it a first and second filial generation (F1 and F2, respectively) based in different families and hybrids. It was found that the trait under study has a high heritability, obtaining in the F2 population strains that consume a minor proportion of the nitrogen sources present in the must. Among these improved strains, strain “686” specially showed a marked drop in the nitrogen consumption, without losing fermentative performance, in synthetic grape must at laboratory level. When using this improved strain to produce wine from a natural grape must (supplemented and non-supplemented with ammonium) at pilot scale under wine cellar conditions, a similar fermentative capacity was obtained between this strain and a widely used commercial strain (EC1118). However, when fermented in a non-supplemented must, improved strain “686” showed the presence of a marked floral aroma absent for EC1118 strain, this difference being probably a direct consequence of its different pattern in amino acid consumption. The combination of the capacity of improved strain “686” to ferment without nitrogen addition and produce floral aromas may be of commercial interest for the wine industry.

scholarly journals Disentangling the genetic bases of Saccharomyces cerevisiae nitrogen consumption and adaptation to low nitrogen environments in wine fermentation

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Eduardo I. Kessi-Pérez ◽  
Jennifer Molinet ◽  
Claudio Martínez
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model Organism ◽  
Industrial Applications ◽  
Nitrogen Utilization ◽  
Wine Fermentation ◽  
Wine Industry ◽  
Economic Losses ◽  
Wine Production ◽  
Low Nitrogen ◽  
Genetic Bases

AbstractThe budding yeast Saccharomyces cerevisiae has been considered for more than 20 years as a premier model organism for biological sciences, also being the main microorganism used in wide industrial applications, like alcoholic fermentation in the winemaking process. Grape juice is a challenging environment for S. cerevisiae, with nitrogen deficiencies impairing fermentation rate and yeast biomass production, causing stuck or sluggish fermentations, thus generating sizeable economic losses for wine industry. In the present review, we summarize some recent efforts in the search of causative genes that account for yeast adaptation to low nitrogen environments, specially focused in wine fermentation conditions. We start presenting a brief perspective of yeast nitrogen utilization under wine fermentative conditions, highlighting yeast preference for some nitrogen sources above others. Then, we give an outlook of S. cerevisiae genetic diversity studies, paying special attention to efforts in genome sequencing for population structure determination and presenting QTL mapping as a powerful tool for phenotype–genotype correlations. Finally, we do a recapitulation of S. cerevisiae natural diversity related to low nitrogen adaptation, specially showing how different studies have left in evidence the central role of the TORC1 signalling pathway in nitrogen utilization and positioned wild S. cerevisiae strains as a reservoir of beneficial alleles with potential industrial applications (e.g. improvement of industrial yeasts for wine production). More studies focused in disentangling the genetic bases of S. cerevisiae adaptation in wine fermentation will be key to determine the domestication effects over low nitrogen adaptation, as well as to definitely proof that wild S. cerevisiae strains have potential genetic determinants for better adaptation to low nitrogen conditions.

scholarly journals Techniques for Mitigating the Effects of Smoke Taint While Maintaining Quality in Wine Production: A Review

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1672
Author(s):  
Ysadora A. Mirabelli-Montan ◽  
Matteo Marangon ◽  
Antonio Graça ◽  
Christine M. Mayr Marangon ◽  
Kerry L. Wilkinson
Keyword(s):  
Global Perspective ◽  
Wine Industry ◽  
Wine Production ◽  
Specific Data ◽  
Main Research ◽  
Initial Severity ◽  
The Subject ◽  
Available Information

Smoke taint has become a prominent issue for the global wine industry as climate change continues to impact the length and extremity of fire seasons around the world. Although the issue has prompted a surge in research on the subject in recent years, no singular solution has yet been identified that is capable of maintaining the quality of wine made from smoke-affected grapes. In this review, we summarize the main research on smoke taint, the key discoveries, as well as the prevailing uncertainties. We also examine methods for mitigating smoke taint in the vineyard, in the winery, and post production. We assess the effectiveness of remediation methods (proposed and actual) based on available research. Our findings are in agreement with previous studies, suggesting that the most viable remedies for smoke taint are still the commercially available activated carbon fining and reverse osmosis treatments, but that the quality of the final treated wines is fundamentally dependent on the initial severity of the taint. In this review, suggestions for future studies are introduced for improving our understanding of methods that have thus far only been preliminarily investigated. We select regions that have already been subjected to severe wildfires, and therefore subjected to smoke taint (particularly Australia and California) as a case study to inform other wine-producing countries that will likely be impacted in the future and suggest specific data collection and policy implementation actions that should be taken, even in countries that have not yet been impacted by smoke taint. Ultimately, we streamline the available information on the topic of smoke taint, apply it to a global perspective that considers the various stakeholders involved, and provide a launching point for further research on the topic.

scholarly journals A SAM-Based Analysis of the Economic Impact of Frauds in the Italian Wine Value Chain

2021 ◽  
Author(s):  
Donato Romano ◽  
Benedetto Rocchi ◽  
Ahmad Sadiddin ◽  
Gianluca Stefani ◽  
Raffaella Zucaro ◽  
...  
Keyword(s):  
Value Chain ◽  
Food System ◽  
Value Added ◽  
Activity Level ◽  
Wine Industry ◽  
Full Time ◽  
Italian Economy ◽  
Wine Production ◽  
Italian Wine ◽  
The Impact

AbstractThe objective of this paper is twofold: firstly, it analyzes the evolution of frauds in the Italian wine value chain over the period 2007–2015, and then, using a properly disaggregated social accounting matrix (SAM) of the Italian economy, it simulates the impact of wine frauds on the national economy in terms of growth, employment, value added and income. The wine industry is the sector most exposed to frauds within the Italian agro-food system accounting for 88% of total value of seized agro-food outputs. Most irregularities (95%) are made by only three agents, specifically individual wineries, bottlers-wholesalers and retailers. We estimated industry-specific SAM multipliers to assess the share of the Italian economy depending on irregular wine production. These activities account for 11.5% of specialized permanent crop farms output and over 25% of wine industry output. This is a sign of vulnerability of the wine industry: should a food scandal/scare determine a drop in consumers’ demand, the negative effect on production activities of these sectors may be large. The SAM was also used to perform an impact analysis adopting a counterfactual approach. Results show a slightly positive increase of value added (6 million euro) along with an overall decrease in the activity level (an output loss of 406 million euro and more than six thousand full time jobs lost). This contractionary effect can be explained with fraud rents. Indeed, the extra-profits from frauds do not activate the economy circular flow as most of them leak out to exogenous accounts such as the public administration and the rest of the world.

DEPENDENCE OF THE CONCENTRATION OF AROMATIC COMPONENTS AND ORGANIC ACIDS IN WHITE TABLE WINE MATERIAL ON THE METHOD OF TECHNOLOGICAL PROCESSING

2019 ◽  
Author(s):  
А.А. АЛЕКСЕЕВА ◽  
Н.М. АГЕЕВА ◽  
В.Е. СТРУКОВА ◽  
Ю.Ф. ЯКУБА ◽  
Л.И. СТРИБИЖЕВА
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Organic Acids ◽  
Methyl Acetate ◽  
Soft Mode ◽  
Higher Alcohols ◽  
Yeast Saccharomyces Cerevisiae ◽  
Grape Must ◽  
Aromatic Components ◽  
Technological Processing ◽  
Active Dry Yeast

Рассмотрено влияние способа технологической обработки белого столового виноматериала на состав и концентрацию компонентов ароматобразующего комплекса и органических кислот. В качестве объекта исследований был белый столовый виноматериал Пино Блан урожая 2018 г. Виноматериал получен по следующей схеме: переработка винограда в мягком режиме путем пневмопрессования, отделение сусла первой фракции, осветление сусла с предварительным охлаждением, внесение подкормки для дрожжей АF1 в количестве 0,9 г/дм3, сбраживание сусла активными сухими дрожжами Saccharomyces cerevisiae раса WT1 (Германия). Установлено, что выдержка виноматериала на дрожжевом осадке привела к снижению концентрации винной и янтарной кислот, количество яблочной и лимонной кислот не изменилось. Отмечено увеличение количества молочной, уксусной, валериановой и изовалериановой кислот. В результате обработки бентонитом снизились концентрации валериановой и изовалериановой кислот. В результате брожения виноградного сусла в присутствии сухих дрожжей образуется большое количество эфиров, определяющих специфические оттенки и формирующих аромат виноматериала. Выдержка виноматериала на дрожжевом осадке способствовала повышению концентрации ацетальдегида, метилацетата, ацеталей, высших спиртов, эфира этилвалериата. Количество других эфиров и терпеновых соединений значительно уменьшилось. Обработка виноматериала бентонитом привела к уменьшению количества ароматобразующих компонентов на 8 16. The influence of the method of technological processing of white table wine material on the composition and concentration of components of the aromatic complex and organic acids is considered. White table wine material Pinot Blanc harvest 2018 was as an object of research. Wine material is obtained according to the following scheme: processing of grapes in the soft mode by pneumomassage, the separation of the wort of the first fraction, wort clarification with preliminary cooling to fertilize for yeast AF1 in an amount of 0,9 g/dm3, the fermentation wort active dry yeast Saccharomyces cerevisiae, race, WT1 (Germany). It was found that the aging of wine material on yeast sediment led to a decrease in the concentration of tartaric and succinic acids, the amount of malic and citric acids has not changed. An increase in the amount of lactic, acetic, valerian and isovaleric acids was noted. Concentrations of valerian and isovaleric acids decreased as a result of treatment by bentonite. A large number of esters, which determine the specific shades and form the aroma of wine material, is formed as a result of fermentation of grape must in the presence of dry yeast. The wine material aging on yeast sediment has contributed to increasing concentration in wine acetaldehyde, and methyl acetate, acetals, higher alcohols, ether of ethylmalonate, a number of other esters and terpenic compounds is significantly reduced. Treatment of wine material by bentonite led to a decrease in the number of aromatic components by 8 16.

scholarly journals Robust design of flash evaporators for must concentration applications in wine production

2018 ◽  
Vol 19 (2) ◽  
pp. 207
Author(s):  
Thomas Quirante ◽  
Yann Ledoux ◽  
Patrick Sebastian
Keyword(s):  
Environmental Impact ◽  
Product Quality ◽  
Robust Design ◽  
Volume Fraction ◽  
Wine Industry ◽  
Design Solution ◽  
Wine Production ◽  
Target Values ◽  
Industry Area

In recent years, flash evaporation processes have received an increased attention in the wine industry for must concentration applications. Specific constraints related to the wine industry area had lead to many improvements of flash evaporators initially designed for seawater desalination. In particular the quality of the vintage, the transportability of the system and the environmental impact are of main interest. Moreover, the preliminary design of such systems must also consider robustness criteria. Obviously, variations of temperatures and flow rates of liquids at the inlet of the process can dramatically impact the quality of the product at the system outlet. In particular, deviations from the target values of temperature and alcoholic volume fraction can lead to a severe degradation of the vintage. As it is common in product design to have multiple performance measures, the robust design problem is addressed using a multi-objective approach. A trade-offs is introduced between two main design objectives: (i) the improvement in overall performance (product quality, transportability, environmental impact and costs) and (ii) the lowering of the sensitivity of the product quality under uncertainty. These main objectives are related to several elementary objectives corresponding to design criteria and a preference aggregation method is used to formulate the two different design objectives. Objectives are linked to weighting parameters values equivalent to priority levels. The selection of the most preferred design solution is discussed according to different trade-off strategies. The generation of the Pareto set is addressed by the non dominated sorting genetic algorithm NGSAII. From computing results, our recommendations concern the compromise between performance and robustness of flash evaporators. In this context, the final alcoholic volume fraction of the wine is the most sensitive parameter, which justify to maintain a high value of evaporative capacity when designing this type of system.

scholarly journals Marketing analysis of the Villány wine region

2014 ◽  
pp. 119-123
Author(s):  
Klára Törökné Kiss
Keyword(s):  
Wine Industry ◽  
Natural Conditions ◽  
Wine Production ◽  
Wine Consumption ◽  
The Future ◽  
Marketing Analysis ◽  
Wine Region

Hungarian wine is not in such a bad position as it is considered. We have outstanding specialists and the country’s natural conditions are also proper. In Hungary, there are wine production and wine consumption has a major traditions. The role of marketing in our wine industry is growing more and more, and this trend is expected to continue in the future. The development of tourism in wine-growing regions and advertising would considerably promote the popularity of Hungarian wine.

scholarly journals The genetic improvement of grapevine cultivars and wine yeast strains: Novel approaches to the ancient art of winemaking

2003 ◽  
Vol 22 (1) ◽  
pp. 31-43
Author(s):  
I. S. Pretorius
Keyword(s):  
Wine Yeast ◽  
Wine Industry ◽  
Market Demand ◽  
Wine Production ◽  
Yeast Strains ◽  
Quantum Leap ◽  
Grape Quality ◽  
The Cost ◽  
Grape Varieties ◽  
Grape Cultivars

The widening gap between wine production and wine consumption, the shift of consumer preferences away from basic commodity wine to top quality wine, and the gruelling competition brought about by economic globalisation call for a total revolution in  the magical world of wine. In the process of transforming the wine industry from a production-driven industry to a market-orientated enterprise, there is an increasing dependence on, amongst others, biotechnological innovation to launch the wine industry with a quantum leap across the formidable market challenges of the 21st century. Market-orientated designer grape cultivars and wine yeast strains are currently being genetically programmed with surgical precision for the cost-competitive production of high quality grapes and wine with relatively minimal resource inputs and a low environmental impact. With regard to Grapevine Biotechnology, this entails the establishment of stress tolerant and disease resistant varieties of Vitis vinifera with increased productivity, efficiency, sustainability and environmental friendliness, especially regarding improved pest and disease control, water use efficiency and grape quality. With regard to Wine Yeast Biotechnology, the emphasis is on the development of Saccharomyces cerevisiae strains with improved fermentation, processing and biopreservation abilities, and capacities for an increase in the wholesomeness and sensory quality of wine. The successful commercialisation of transgenic grape cultivars and wine yeasts depends on a number of scientific, technical, safety, ethical, legal, economic and marketing factors, and it therefore will be unwise to entertain high expectations in the short term. However, in the light of the phenomenal potential advantages of tailor-made grape varieties and yeast strains, it would be equally self-destructive in the long term if this strategically important “life insurance policy” is not taken out by the wine industry. This overview highlights the most important examples of the way in which V. vinifera grape varieties and S. cerevisiae wine yeast strains are currently being designed with surgical precision on the basis of market demand for the cost-effective, sustainable and environmentally friendly production of healthy, top quality grapes and wine.

scholarly journals Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae.

1980 ◽  
Vol 85 (1) ◽  
pp. 108-115 ◽  
Author(s):  
C J Rivin ◽  
W L Fangman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cycle Length ◽  
Replication Fork ◽  
Nitrogen Sources ◽  
S Phase ◽  
Phase Duration ◽  
Yeast Saccharomyces Cerevisiae ◽  
Origin Activation ◽  
Cell Cycle Length

When the growth rate of the yeast Saccharomyces cerevisiae is limited with various nitrogen sources, the duration of the S phase is proportional to cell cycle length over a fourfold range of growth rates (C.J. Rivin and W. L. Fangman, 1980, J. Cell Biol. 85:96-107). Molecular parameters of the S phases of these cells were examined by DNA fiber autoradiography. Changes in replication fork rate account completely for the changes in S-phase duration. No changes in origin-to-origin distances were detected. In addition, it was found that while most adjacent replication origins are activated within a few minutes of each other, new activations occur throughout the S phase.

scholarly journals A genetic analysis reveals low prevalence of phytoplasma infection in Hyalesthes obsoletus Signoret, vector of 'bois noir', in SW-Germany

2015 ◽  
Author(s):  
Gabriele Schiro ◽  
Johannes Fahrentrapp ◽  
Florian Hartig ◽  
Bernd Panassiti
Keyword(s):  
Temporal Distribution ◽  
West Germany ◽  
Economic Losses ◽  
Type I ◽  
Wine Production ◽  
Bois Noir ◽  
Vector Population ◽  
Spatio Temporal ◽  
Low Prevalence ◽  
Hyalesthes Obsoletus

Bois Noir is a grapevine disease responsible for severe economic losses in wine production. Bois Noir is caused by Candidatus Phytoplasma solani, cell wall-less bacteria belonging to the taxonomic group 16Sr-XII-A. In Germany, they are known to be vectored from plant to plant by the cixiid Hyalesthes obsoletus, but so far the prevalence of the disease in the vector population, as well as its spatio-temporal distribution is poorly understood. We therefore analyzed infections of H. obsoletus collected in different vineyards in Baden (South-Western Germany) with quantitative real-time PCR. From 125 analyzed individuals, only five were infected with Ca. Phytoplasma solani. All infected individuals were colonized by Ca. Phytoplasma solani type I which is associated with the host plant Urtica dioica (stinging nettle). More research is needed to understand the reasons of this surprisingly low prevalence of Bois Noir in the population of H. obsoletus in South-West Germany.

Climate change impacts on viticulture in Italy

2021 ◽  
Author(s):  
Laura Massano ◽  
Giorgia Fosser ◽  
Marco Gaetani
Keyword(s):  
Climate Change ◽  
Horizontal Resolution ◽  
Wine Industry ◽  
Added Value ◽  
Extreme Weather Events ◽  
Wine Production ◽  
Bioclimatic Indices ◽  
Impact Of Climate Change ◽  
Impact Studies ◽  
The Impact

<p>In Italy the wine industry is an economic asset representing the 8% of the annual turnover of the Food & Beverage sector, according to Unicredit Industry Book 2019. Viticulture is strongly influenced by weather and climate, and winegrowers in Europe have already experienced the impact of climate change in terms of more frequent drought periods, warmer and longer growing seasons and an increased frequency of weather extremes. These changes impact on both yield production and wine quality.</p><p>Our study aims to understand the impact of climate change on wine production, to estimate the risks associated with climate factors and to suggest appropriate adaptation measurement. The weather variables that most influence grape growth are: temperature, precipitation and evapotranspiration. Starting for these variables we calculate a range of bioclimatic indices, selected following the International Organisation of Vine and Wine Guidelines (OIV), and correlate these with wine productivity data. According to the values of different indices it is possible to determine the more suitable areas for wine production, where we expect higher productivity, although the climate is not the only factor influencing yield.</p><p>Using the convection-permitting models (CPMs – 2.2 horizontal resolution) we investigate how the bioclimatic indices changed in the last 20 years, and the impact of this change on grapes productivity. We look at possible climate trends and at the variation in the frequency distribution of extreme weather events. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of surface and orography field, explicitly resolve deep convection and show an improved representation of extremes events. In our study, we compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to evaluate the possible added value of high resolution models for impact studies. To compare models' output to observation the same analysis it carried out using E-OBS dataset.</p><p>Through our impact study, we aim to provide a tool that winegrower and stakeholders involved in the wine business can use to make their activities more sustainable and more resilient to climate change.</p>

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