scholarly journals Optimized MALDI TOF Mass Spectrometry Identification of Francisella tularensis Subsp. holarctica

2020 ◽  
Vol 8 (8) ◽  
pp. 1143
Author(s):  
Sofiane Regoui ◽  
Aurélie Hennebique ◽  
Thomas Girard ◽  
Sandrine Boisset ◽  
Yvan Caspar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Francisella Tularensis ◽  
Healthcare Management ◽  
Accurate Identification ◽  
Tularensis Subsp ◽  
Gram Negative ◽  
Maldi Tof Mass Spectrometry ◽  
Human Samples ◽  
Maldi Tof ◽  
Mass Spectrometry Identification

Francisella tularensis is a tier 1 agent causing the zoonosis tularemia. This highly infectious Gram-negative bacterium is occasionally isolated from human samples (especially blood samples) in routine clinical microbiology laboratories. A rapid and accurate method for identifying this pathogen is needed in order to optimize the infected patient’s healthcare management and prevent contamination of the laboratory personnel. MALDI TOF mass spectrometry has become the gold standard for the rapid identification of most human pathogens. However, F. tularensis identification using such technology and commercially available databases is currently considered unreliable. Real-time PCR-based methods for rapid detection and accurate identification of F. tularensis are not available in many laboratories. As a national reference center for tularemia, we developed a MALDI TOF database allowing accurate identification of the species F. tularensis and its differentiation from the closely related neighbor species F. tularensis subsp. novicida and F. philomiragia. The sensitivity and specificity of this database were validated by testing 71 F. tularensis strains and 165 strains from 63 species not belonging to the Francisella genus. We obtained accurate identification at the species level and differentiation of all the tested bacterial strains. In particular, F. tularensis could be accurately differentiated from other small Gram-negative bacilli occasionally isolated from human samples, including species of the HACEK group and Brucella melitensis.

scholarly journals Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

2016 ◽  
Vol 120 ◽  
pp. 68-71 ◽  
Author(s):  
Gerald Larrouy-Maumus ◽  
Abigail Clements ◽  
Alain Filloux ◽  
Ronan R. McCarthy ◽  
Serge Mostowy
Keyword(s):  
Mass Spectrometry ◽  
Lipid A ◽  
Direct Detection ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1

2021 ◽  
Author(s):  
Pascal Mülner ◽  
Elisa Schwarz ◽  
Kristin Dietel ◽  
Stefanie Herfort ◽  
Jennifer Jähne ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Characteristic Feature ◽  
Fungal Pathogens ◽  
Paenibacillus Polymyxa ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Antifungal Activities ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Volatile Organic

Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnol-ogy. Lipopeptides produced by such organisms play a major role in their inactivation potential. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by Paenibacillus polymyxa strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variabil-ity. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains novel, but not yet completely characterized polymyxin species were detected, which pos-sibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins attain increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC-MS approach using different cultivation media. Production of volatile organic com-pounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an effi-cient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of Paenibacilli is the formation of volatile pyrazine derivatives.

Quick identification and epidemiological characterization of Francisella tularensis by MALDI-TOF mass spectrometry

2020 ◽  
Vol 177 ◽  
pp. 106055
Author(s):  
Irene López-Ramos ◽  
Marta Hernández ◽  
David Rodríguez-Lázaro ◽  
María P. Gutiérrez ◽  
Pilar Zarzosa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Francisella Tularensis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Epidemiological Characterization

Fast and accurate identification of molds and yeast-like fungi directly from positive blood cultures by MALDI-TOF mass spectrometry

2016 ◽  
Vol 26 (2) ◽  
pp. e36-e37
Author(s):  
J.N. De Almeida Júnior ◽  
J. Sztajnbok ◽  
A. Da Silva Júnior ◽  
A. Galastri ◽  
L. Bissoli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Blood Cultures ◽  
Accurate Identification ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

MALDI-TOF mass spectrometry identification of filamentous fungi in the clinical laboratory

Mycoses ◽  
2013 ◽  
Vol 57 (3) ◽  
pp. 135-140 ◽  
Author(s):  
Stéphane Ranque ◽  
Anne-Cécile Normand ◽  
Carole Cassagne ◽  
Jean-Benjamin Murat ◽  
Nathalie Bourgeois ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Filamentous Fungi ◽  
Clinical Laboratory ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Mass Spectrometry Identification

Rapid MALDI-TOF mass spectrometry identification of Leptospira organisms: A reply

2012 ◽  
Vol 159 (3-4) ◽  
pp. 544 ◽  
Author(s):  
Zoheira Djelouadji ◽  
Véronique Roux ◽  
Didier Raoult ◽  
Angeli Kodjo ◽  
Michel Drancourt
Keyword(s):  
Mass Spectrometry ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Mass Spectrometry Identification

scholarly journals MICROBIAL COMPOSITION OF VARIOUS SURFACES OF SKIN DURING DEVELOPMENT OF ATOPIC DERMATITIS BASED ON DATA FROM MALDI-TOF MASS-SPECTROMETRY IDENTIFICATION METHOD

2016 ◽  
pp. 30-36
Author(s):  
Yu. A. Tyurin ◽  
R. S. Fassakhov ◽  
T. V. Grigorieva ◽  
I. G. Mustafin
Keyword(s):  
Mass Spectrometry ◽  
Atopic Dermatitis ◽  
Lower Limb ◽  
High Frequency ◽  
Frequency Of Occurrence ◽  
Healthy Individuals ◽  
Microbial Composition ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Mass Spectrometry Identification

Aim. Study transformation of skin microflora during development of atopic dermatitis. Materials and methods. 45 patients with various forms of atopic dermatitis (AtD) were examined. Control group consisted of 26 healthy individuals. The strains were cultivated on elective nutrient media. Identification of the isolated strains was carried out by MALDI-TOF mass-spectrometry method. Results. A low frequency of occurrence of taxon Staphylococcus epidermidis on face skin and high frequency of occurrence of Staphylococcus aureus on upper and lower limb skin was established for AtD patients compared with healthy individuals. The frequency of occurrence of proteolytically active isolates of S. aureus in AtD patients was 3 times higher than in healthy carriers of this taxon. Taxons of microorganisms not inherent to healthy individuals such as Bacillus mycoides, Pseudomonas putida, Pseudomonas radiobacter were isolated on lower limb and neck skin of AtD patients. A high frequency of occurrence of Cryptococcus satoi, Candida albicans, Malassezia globosa fungi was noted. Conclusion. A decrease of barrier functions of skin during AtD facilitates contamination of patients’ skin with rare bacterial taxons and fungi. One of the possible mechanisms of suppression of immune competent cell functions could be proteolytic enzymes of S. aureus.

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